Restriction map for
pF1KB8585
(Circular) (Six-base) MAPSORT of: pF1KB8585.seq Check: 1247 from: 1 to: 5501
pF1KB8585 5501 bp
With 182 enzymes: SgfI *
November 4, 2009 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1089 1089
Size: 5501
AccI GT'mk_AC
Cuts at: 344 2539 344
Size: 2195 3306
Acc65I G'GTAC_C
Cuts at: 2517 2517
Size: 5501
AclI AA'CG_TT
Cuts at: 816 5341 816
Size: 4525 976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1121 2013 3326 3758 4111 4773 553
Size: 568 892 1313 432 353 662 1281
Fragments arranged by size:
1313 1281 892 662 568 432 353
AflII C'TTAA_G
Cuts at: 1848 1848
Size: 5501
AflIII A'CryG_T
Cuts at: 2853 4653 4934 2853
Size: 1800 281 3420
Fragments arranged by size:
3420 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1398 1398
Size: 5501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2250 2250
Size: 5501
AlwNI CAG_nnn'CTG
Cuts at: 2806 4244 2806
Size: 1438 4063
ApaLI G'TGCA_C
Cuts at: 2126 4339 2126
Size: 2213 3288
ApoI r'AATT_y
Cuts at: 758 774 925 1491 1743 1921 2273 2505 4854
Size: 16 151 566 252 178 352 232 2349
Cuts at: 4854 5110 758
Size: 256 1149
Fragments arranged by size:
2349 1149 566 352 256 252 232 178
151 16
AseI AT'TA_AT
Cuts at: 20 1172 20
Size: 1152 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5501
AvaI C'yCGr_G
Cuts at: 2521 2521
Size: 5501
BamHI G'GATC_C
Cuts at: 2526 2526
Size: 5501
BanI G'GyrC_C
Cuts at: 2517 2931 3179 3214 2517
Size: 414 248 35 4804
Fragments arranged by size:
4804 414 248 35
BanII G_rGCy'C
Cuts at: 2515 3545 2515
Size: 1030 4471
BbeI G_GCGC'C
Cuts at: 2935 3183 2935
Size: 248 5253
BbsI GAAGACnn'nnnn_
Cuts at: 1544 2369 1544
Size: 825 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5467
BciVI GTATCCnnnnn_n'
Cuts at: 1509 3399 4455 5501 1509
Size: 1890 1056 1046 1509
Fragments arranged by size:
1890 1509 1056 1046
BclI T'GATC_A
Cuts at: 440 1820 1997 2100 3021 440
Size: 1380 177 103 921 2920
Fragments arranged by size:
2920 1380 921 177 103
BglI GCCn_nnn'nGGC
Cuts at: 169 1580 169
Size: 1411 4090
BglII A'GATC_T
Cuts at: 384 3016 5053 384
Size: 2632 2037 832
Fragments arranged by size:
2632 2037 832
BlpI GC'TnA_GC
Cuts at: 2613 2613
Size: 5501
Bme1580I G_kGCm'C
Cuts at: 2070 2130 3126 3219 4343 2070
Size: 60 996 93 1124 3228
Fragments arranged by size:
3228 1124 996 93 60
BmrI ACTGGGnnnn_n'
Cuts at: 2713 2794 3129 5273 2713
Size: 81 335 2144 2941
Fragments arranged by size:
2941 2144 335 81
BmtI G_CTAG'C
Cuts at: 1984 1984
Size: 5501
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 3868 2383
Size: 1485 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 2449 919
Size: 1530 3971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 866 1451 2677 3556 4270 4568 558
Size: 308 585 1226 879 714 298 1491
Fragments arranged by size:
1491 1226 879 714 585 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 2491 5205 2491
Size: 2714 2787
BsaAI yAC'GTr
Cuts at: 2854 2854
Size: 5501
BsaBI GATnn'nnATC
Cuts at: 6 3015 5052 6
Size: 3009 2037 455
Fragments arranged by size:
3009 2037 455
BsaHI Gr'CG_yC
Cuts at: 2932 3180 3882 5378 2932
Size: 248 702 1496 3055
Fragments arranged by size:
3055 1496 702 248
BsaWI w'CCGG_w
Cuts at: 266 2690 3211 3889 4300 4447 4721 266
Size: 2424 521 678 411 147 274 1046
Fragments arranged by size:
2424 1046 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2185 2185
Size: 5501
BseYI C'CCAG_C
Cuts at: 1062 1879 2926 3684 4349 4757 1062
Size: 817 1047 758 665 408 1806
Fragments arranged by size:
1806 1047 817 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1304 2278 313
Size: 991 974 3536
Fragments arranged by size:
3536 991 974
BsiEI CG_ry'CG
Cuts at: 104 3043 3089 4319 104
Size: 2939 46 1230 1286
Fragments arranged by size:
2939 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1872 2122 2130 2515 3293 3483 4343 1872
Size: 250 8 385 778 190 860 3030
Fragments arranged by size:
3030 860 778 385 250 190 8
BsmI GAATG_Cn'
Cuts at: 701 701
Size: 5501
Bsp1286I G_dGCh'C
Cuts at: 1872 2070 2122 2130 2515 3126 3219 3293 3483
Size: 198 52 8 385 611 93 74 190
Cuts at: 3483 3545 4343 1872
Size: 62 798 3030
Fragments arranged by size:
3030 798 611 385 198 190 93 74
62 52 8
BspEI T'CCGG_A
Cuts at: 266 266
Size: 5501
BspHI T'CATG_A
Cuts at: 1902 2043 3933 1902
Size: 141 1890 3470
Fragments arranged by size:
3470 1890 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1089 2551 2765 3067 3448 1089
Size: 1462 214 302 381 3142
Fragments arranged by size:
3142 1462 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3793 3847 5331 5496 50
Size: 3743 54 1484 165 55
Fragments arranged by size:
3743 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1004 3413 1004
Size: 2409 3092
BsrFI r'CCGG_y
Cuts at: 3499 3680 3499
Size: 181 5320
BsrGI T'GTAC_A
Cuts at: 399 399
Size: 5501
BssSI C'ACGA_G
Cuts at: 3772 4480 3772
Size: 708 4793
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 2878 1876
Size: 1002 4499
BstBI TT'CG_AA
Cuts at: 555 3862 555
Size: 3307 2194
BstYI r'GATC_y
Cuts at: 1 384 2526 2993 3016 3351 3597 3903 3915
Size: 383 2142 467 23 335 246 306 12
Cuts at: 3915 4001 5053 1
Size: 86 1052 449
Fragments arranged by size:
2142 1052 467 449 383 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 131 131
Size: 5501
BtgI C'CryG_G
Cuts at: 519 644 519
Size: 125 5376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3451 3632 3795 3451
Size: 181 163 5157
Fragments arranged by size:
5157 181 163
BtsI GCAGTG_nn'
Cuts at: 1690 2744 1690
Size: 1054 4447
DraI TTT'AAA
Cuts at: 2336 2500 2336
Size: 164 5337
DrdI GACnn_nn'nnGTC
Cuts at: 3207 4551 3207
Size: 1344 4157
EaeI y'GGCC_r
Cuts at: 311 3086 3260 3651 3678 5023 311
Size: 2775 174 391 27 1345 789
Fragments arranged by size:
2775 1345 789 391 174 27
EagI C'GGCC_G
Cuts at: 3086 3086
Size: 5501
EarI CTCTTCn'nnn_
Cuts at: 193 2030 2140 3524 3734 193
Size: 1837 110 1384 210 1960
Fragments arranged by size:
1960 1837 1384 210 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3709 4451 4597 5311 3709
Size: 742 146 714 3899
Fragments arranged by size:
3899 742 714 146
EcoICRI GAG'CTC
Cuts at: 2513 2513
Size: 5501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 919 1121 2013 2449 3326 3758 4111 4773
Size: 366 202 892 436 877 432 353 662
Cuts at: 4773 553
Size: 1281
Fragments arranged by size:
1281 892 877 662 436 432 366 353
202
EcoNI CCTnn'n_nnAGG
Cuts at: 596 596
Size: 5501
EcoO109I rG'GnC_Cy
Cuts at: 1611 2640 1611
Size: 1029 4472
EcoRI G'AATT_C
Cuts at: 1743 1921 2505 1743
Size: 178 584 4739
Fragments arranged by size:
4739 584 178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 3520 2278
Size: 1242 4259
FspI TGC'GCA
Cuts at: 3282 3282
Size: 5501
HaeII r_GCGC'y
Cuts at: 2935 3183 4413 5184 2935
Size: 248 1230 771 3252
Fragments arranged by size:
3252 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 930 962 1043 1075 1135 1167 1384 1416 1500
Size: 32 81 32 60 32 217 32 84
Cuts at: 1500 1532 3343 3373 3375 3405 930
Size: 32 1811 30 2 30 3026
Fragments arranged by size:
3026 1811 217 84 81 60 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1326 2540 1326
Size: 1214 4287
HindIII A'AGCT_T
Cuts at: 220 220
Size: 5501
KasI G'GCGC_C
Cuts at: 2931 3179 2931
Size: 248 5253
KpnI G_GTAC'C
Cuts at: 2521 2521
Size: 5501
MluI A'CGCG_T
Cuts at: 4934 4934
Size: 5501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 429 776 4260 4444 429
Size: 347 3484 184 1486
Fragments arranged by size:
3484 1486 347 184
MscI TGG'CCA
Cuts at: 313 3262 313
Size: 2949 2552
MslI CAynn'nnrTG
Cuts at: 980 3490 3617 980
Size: 2510 127 2864
Fragments arranged by size:
2864 2510 127
MspA1I CmG'CkG
Cuts at: 1066 1677 1722 2612 2926 3286 4070 4315 1066
Size: 611 45 890 314 360 784 245 2252
Fragments arranged by size:
2252 890 784 611 360 314 245 45
NaeI GCC'GGC
Cuts at: 3682 3682
Size: 5501
NarI GG'CG_CC
Cuts at: 2932 3180 2932
Size: 248 5253
NcoI C'CATG_G
Cuts at: 644 644
Size: 5501
NdeI CA'TA_TG
Cuts at: 1917 1917
Size: 5501
NgoMIV G'CCGG_C
Cuts at: 3680 3680
Size: 5501
NheI G'CTAG_C
Cuts at: 1980 1980
Size: 5501
NspI r_CATG'y
Cuts at: 244 1134 2554 3054 4657 244
Size: 890 1420 500 1603 1088
Fragments arranged by size:
1603 1420 1088 890 500
PciI A'CATG_T
Cuts at: 4653 4653
Size: 5501
PfoI T'CCnGG_A
Cuts at: 481 481
Size: 5501
PmeI GTTT'AAAC
Cuts at: 2500 2500
Size: 5501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 5469
PpuMI rG'GwC_Cy
Cuts at: 1611 1611
Size: 5501
PsiI TTA'TAA
Cuts at: 1448 1448
Size: 5501
PstI C_TGCA'G
Cuts at: 2548 3233 2548
Size: 685 4816
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5501
PvuII CAG'CTG
Cuts at: 1722 2926 3286 1722
Size: 1204 360 3937
Fragments arranged by size:
3937 1204 360
RsrII CG'GwC_CG
Cuts at: 3696 3696
Size: 5501
SacI G_AGCT'C
Cuts at: 2515 2515
Size: 5501
SalI G'TCGA_C
Cuts at: 2538 2538
Size: 5501
SapI GCTCTTCn'nnn_
Cuts at: 3524 3734 3524
Size: 210 5291
SbfI CC_TGCA'GG
Cuts at: 2548 2548
Size: 5501
ScaI AGT'ACT
Cuts at: 1387 1627 5018 1387
Size: 240 3391 1870
Fragments arranged by size:
3391 1870 240
SexAI A'CCwGG_T
Cuts at: 1472 1472
Size: 5501
SfcI C'TryA_G
Cuts at: 33 864 1106 1532 2544 3229 4197 4388 33
Size: 831 242 426 1012 685 968 191 1146
Fragments arranged by size:
1146 1012 968 831 685 426 242 191
SfoI GGC'GCC
Cuts at: 2933 3181 2933
Size: 248 5253
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5501
SmaI CCC'GGG
Cuts at: 2523 2523
Size: 5501
SmlI C'TyrA_G
Cuts at: 537 881 1466 1848 2656 3571 4285 4547 537
Size: 344 585 382 808 915 714 262 1491
Fragments arranged by size:
1491 915 808 714 585 382 344 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5501
SphI G_CATG'C
Cuts at: 2554 3054 2554
Size: 500 5001
SspI AAT'ATT
Cuts at: 1341 1341
Size: 5501
StyI C'CwwG_G
Cuts at: 424 644 2635 2989 424
Size: 220 1991 354 2936
Fragments arranged by size:
2936 1991 354 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 678 1388 3084 3217 3676 3885 678
Size: 710 1696 133 459 209 2294
Fragments arranged by size:
2294 1696 710 459 209 133
TatI w'GTAC_w
Cuts at: 399 654 1385 1625 5016 399
Size: 255 731 240 3391 884
Fragments arranged by size:
3391 884 731 255 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 2049 2081 5018 5050 362
Size: 32 1655 32 2937 32 813
Fragments arranged by size:
2937 1655 813 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3298 3298
Size: 5501
XbaI T'CTAG_A
Cuts at: 1120 1224 2532 1120
Size: 104 1308 4089
Fragments arranged by size:
4089 1308 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1491 2996 1491
Size: 1505 3996
XmaI C'CCGG_G
Cuts at: 2521 2521
Size: 5501
XmnI GAAnn'nnTTC
Cuts at: 2026 2026
Size: 5501
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI Bpu10I BsaXI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI