Restriction map for
pF1KB8584
(Circular) (Six-base) MAPSORT of: pF1KB8584.seq Check: 7818 from: 1 to: 5432
pF1KB8584 5432 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 1463 1463
Size: 5432
AccI GT'mk_AC
Cuts at: 374 2470 374
Size: 2096 3336
Acc65I G'GTAC_C
Cuts at: 2448 2448
Size: 5432
AclI AA'CG_TT
Cuts at: 872 5272 872
Size: 4400 1032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1501 1594 1843 2117 3257 3689 4436 4704
Size: 183 93 249 274 1140 432 747 268
Cuts at: 4704 1318
Size: 2046
Fragments arranged by size:
2046 1140 747 432 274 268 249 183
93
AflIII A'CryG_T
Cuts at: 878 2784 3888 4865 878
Size: 1906 1104 977 1445
Fragments arranged by size:
1906 1445 1104 977
AgeI A'CCGG_T
Cuts at: 3820 3820
Size: 5432
AleI CACnn'nnGTG
Cuts at: 566 566
Size: 5432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1006 1038 1006
Size: 32 5400
AlwNI CAG_nnn'CTG
Cuts at: 648 1227 1551 1806 1821 2737 4304 648
Size: 579 324 255 15 916 1567 1776
Fragments arranged by size:
1776 1567 916 579 324 255 15
ApaI G_GGCC'C
Cuts at: 1063 2051 1063
Size: 988 4444
ApaLI G'TGCA_C
Cuts at: 4202 4202
Size: 5432
ApoI r'AATT_y
Cuts at: 394 427 493 2436 4785 5041 394
Size: 33 66 1943 2349 256 785
Fragments arranged by size:
2349 1943 785 256 66 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
AvaI C'yCGr_G
Cuts at: 2452 2452
Size: 5432
BamHI G'GATC_C
Cuts at: 1122 1131 2457 1122
Size: 9 1326 4097
Fragments arranged by size:
4097 1326 9
BanI G'GyrC_C
Cuts at: 2251 2448 2862 3110 3145 2251
Size: 197 414 248 35 4538
Fragments arranged by size:
4538 414 248 197 35
BanII G_rGCy'C
Cuts at: 734 1063 2051 2446 3476 734
Size: 329 988 395 1030 2690
Fragments arranged by size:
2690 1030 988 395 329
BbeI G_GCGC'C
Cuts at: 2866 3114 2866
Size: 248 5184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 983 949
Size: 34 5398
BciVI GTATCCnnnnn_n'
Cuts at: 921 3330 4091 5432 921
Size: 2409 761 1341 921
Fragments arranged by size:
2409 1341 921 761
BclI T'GATC_A
Cuts at: 266 2952 266
Size: 2686 2746
BfrBI ATG'CAT
Cuts at: 1309 1309
Size: 5432
BglII A'GATC_T
Cuts at: 2368 2947 4984 2368
Size: 579 2037 2816
Fragments arranged by size:
2816 2037 579
BlpI GC'TnA_GC
Cuts at: 1175 2544 1175
Size: 1369 4063
Bme1580I G_kGCm'C
Cuts at: 1063 1400 1895 2051 2254 3057 3150 4206 1063
Size: 337 495 156 203 803 93 1056 2289
Fragments arranged by size:
2289 1056 803 495 337 203 156 93
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1517 2644 2725 3060 5204 1003
Size: 81 433 1127 81 335 2144 1231
Fragments arranged by size:
2144 1231 1127 433 335 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3799 3799
Size: 5432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 929 1039 2176 894
Size: 35 110 1137 4150
Fragments arranged by size:
4150 1137 110 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 2608 3487 3979 4277 728
Size: 1880 879 492 298 1883
Fragments arranged by size:
1883 1880 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2152 5136 2152
Size: 2984 2448
BsaAI yAC'GTr
Cuts at: 1879 2785 1879
Size: 906 4526
BsaBI GATnn'nnATC
Cuts at: 6 2946 4983 6
Size: 2940 2037 455
Fragments arranged by size:
2940 2037 455
BsaHI Gr'CG_yC
Cuts at: 1460 2863 3111 3813 5309 1460
Size: 1403 248 702 1496 1583
Fragments arranged by size:
1583 1496 1403 702 248
BsaWI w'CCGG_w
Cuts at: 2621 3142 3820 4094 4241 4652 2621
Size: 521 678 274 147 411 3401
Fragments arranged by size:
3401 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1798 1801 1798
Size: 3 5429
BseYI C'CCAG_C
Cuts at: 1423 2857 3615 4192 4688 1423
Size: 1434 758 577 496 2167
Fragments arranged by size:
2167 1434 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1576 1654 1876 2242 1576
Size: 78 222 366 4766
Fragments arranged by size:
4766 366 222 78
BsiEI CG_ry'CG
Cuts at: 104 2974 3020 4228 104
Size: 2870 46 1208 1308
Fragments arranged by size:
2870 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 256 734 1176 2446 3224 3414 4206 256
Size: 478 442 1270 778 190 792 1482
Fragments arranged by size:
1482 1270 792 778 478 442 190
BsmI GAATG_Cn'
Cuts at: 629 1311 2111 629
Size: 682 800 3950
Fragments arranged by size:
3950 800 682
Bsp1286I G_dGCh'C
Cuts at: 256 734 1063 1176 1400 1895 2051 2254 2446
Size: 478 329 113 224 495 156 203 192
Cuts at: 2446 3057 3150 3224 3414 3476 4206 256
Size: 611 93 74 190 62 730 1482
Fragments arranged by size:
1482 730 611 495 478 329 224 203
192 190 156 113 93 74 62
BspEI T'CCGG_A
Cuts at: 4652 4652
Size: 5432
BspHI T'CATG_A
Cuts at: 4608 4608
Size: 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2482 2696 2998 3379 2482
Size: 214 302 381 4535
Fragments arranged by size:
4535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1743 3724 3778 5262 5427 50
Size: 1693 1981 54 1484 165 55
Fragments arranged by size:
1981 1693 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3344 3344
Size: 5432
BsrFI r'CCGG_y
Cuts at: 3430 3611 3820 3430
Size: 181 209 5042
Fragments arranged by size:
5042 209 181
BsrGI T'GTAC_A
Cuts at: 995 995
Size: 5432
BssSI C'ACGA_G
Cuts at: 3703 4061 3703
Size: 358 5074
BstAPI GCAn_nnn'nTGC
Cuts at: 1554 1683 1806 2809 1554
Size: 129 123 1003 4177
Fragments arranged by size:
4177 1003 129 123
BstBI TT'CG_AA
Cuts at: 3793 3793
Size: 5432
BstYI r'GATC_y
Cuts at: 1 1122 1131 2368 2457 2924 2947 3282 3528
Size: 1121 9 1237 89 467 23 335 246
Cuts at: 3528 4540 4626 4638 4984 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1237 1121 1012 467 449 346 335 246
89 86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 593 980 2033 593
Size: 387 1053 3992
Fragments arranged by size:
3992 1053 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2255 3382 3563 3726 2255
Size: 1127 181 163 3961
Fragments arranged by size:
3961 1127 181 163
BtsI GCAGTG_nn'
Cuts at: 1400 2675 1400
Size: 1275 4157
DraI TTT'AAA
Cuts at: 443 2431 443
Size: 1988 3444
DrdI GACnn_nn'nnGTC
Cuts at: 3138 3996 3138
Size: 858 4574
EaeI y'GGCC_r
Cuts at: 2262 3017 3191 3582 3609 4954 2262
Size: 755 174 391 27 1345 2740
Fragments arranged by size:
2740 1345 755 391 174 27
EagI C'GGCC_G
Cuts at: 3017 3017
Size: 5432
EarI CTCTTCn'nnn_
Cuts at: 126 297 302 353 1256 3455 3665 126
Size: 171 5 51 903 2199 210 1893
Fragments arranged by size:
2199 1893 903 210 171 51 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3640 3950 4096 5242 3640
Size: 310 146 1146 3830
Fragments arranged by size:
3830 1146 310 146
EcoICRI GAG'CTC
Cuts at: 732 2444 732
Size: 1712 3720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 929 1039 1318 1501 1594 1843 2117 2176
Size: 35 110 279 183 93 249 274 59
Cuts at: 2176 3257 3689 4436 4704 894
Size: 1081 432 747 268 1622
Fragments arranged by size:
1622 1081 747 432 279 274 268 249
183 110 93 59 35
EcoO109I rG'GnC_Cy
Cuts at: 1059 1077 2047 2048 2311 2338 2571 1059
Size: 18 970 1 263 27 233 3920
Fragments arranged by size:
3920 970 263 233 27 18 1
EcoRI G'AATT_C
Cuts at: 2436 2436
Size: 5432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 709 3451 709
Size: 2742 2690
FspI TGC'GCA
Cuts at: 3213 3213
Size: 5432
HaeII r_GCGC'y
Cuts at: 2866 3114 4136 5115 2866
Size: 248 1022 979 3183
Fragments arranged by size:
3183 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 440 472 1311 1343 1366 1398 3274
Size: 32 283 32 839 32 23 32 1876
Cuts at: 3274 3304 3306 3336 125
Size: 30 2 30 2221
Fragments arranged by size:
2221 1876 839 283 32 32 32 32
30 30 23 2
HincII GTy'rAC
Cuts at: 2471 2471
Size: 5432
KasI G'GCGC_C
Cuts at: 2862 3110 2862
Size: 248 5184
KpnI G_GTAC'C
Cuts at: 2452 2452
Size: 5432
MluI A'CGCG_T
Cuts at: 4865 4865
Size: 5432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1082 1514 4103 4287 1082
Size: 432 2589 184 2227
Fragments arranged by size:
2589 2227 432 184
MscI TGG'CCA
Cuts at: 3193 3193
Size: 5432
MslI CAynn'nnrTG
Cuts at: 112 274 566 766 3421 3548 112
Size: 162 292 200 2655 127 1996
Fragments arranged by size:
2655 1996 292 200 162 127
MspA1I CmG'CkG
Cuts at: 316 793 1587 1593 1605 1614 1806 1957 2064
Size: 477 794 6 12 9 192 151 107
Cuts at: 2064 2543 2857 3217 4230 4475 316
Size: 479 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 794 479 477 360 314 245
192 151 107 12 9 6
NaeI GCC'GGC
Cuts at: 3613 3613
Size: 5432
NarI GG'CG_CC
Cuts at: 2863 3111 2863
Size: 248 5184
NdeI CA'TA_TG
Cuts at: 1206 1206
Size: 5432
NgoMIV G'CCGG_C
Cuts at: 3611 3611
Size: 5432
NsiI A_TGCA'T
Cuts at: 1311 1311
Size: 5432
NspI r_CATG'y
Cuts at: 587 882 2485 2985 3892 587
Size: 295 1603 500 907 2127
Fragments arranged by size:
2127 1603 907 500 295
PciI A'CATG_T
Cuts at: 878 3888 878
Size: 3010 2422
PflMI CCAn_nnn'nTGG
Cuts at: 1227 1227
Size: 5432
PfoI T'CCnGG_A
Cuts at: 923 923
Size: 5432
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5432
PmlI CAC'GTG
Cuts at: 1879 1879
Size: 5432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 4571
Size: 32 5400
PpuMI rG'GwC_Cy
Cuts at: 1077 2311 1077
Size: 1234 4198
PspOMI G'GGCC_C
Cuts at: 1059 2047 1059
Size: 988 4444
PstI C_TGCA'G
Cuts at: 653 1673 1706 2400 2479 3164 653
Size: 1020 33 694 79 685 2921
Fragments arranged by size:
2921 1020 694 685 79 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5432
PvuII CAG'CTG
Cuts at: 316 1593 1605 1614 1806 1957 2064 2857 3217
Size: 1277 12 9 192 151 107 793 360
Cuts at: 3217 316
Size: 2531
Fragments arranged by size:
2531 1277 793 360 192 151 107 12
9
RsrII CG'GwC_CG
Cuts at: 3627 3627
Size: 5432
SacI G_AGCT'C
Cuts at: 734 2446 734
Size: 1712 3720
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5432
SapI GCTCTTCn'nnn_
Cuts at: 3455 3665 3455
Size: 210 5222
SbfI CC_TGCA'GG
Cuts at: 2479 2479
Size: 5432
ScaI AGT'ACT
Cuts at: 4949 4949
Size: 5432
SfcI C'TryA_G
Cuts at: 33 220 649 1669 1702 1735 1768 2396 2475
Size: 187 429 1020 33 33 33 628 79
Cuts at: 2475 3160 4153 4344 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1020 993 685 628 429 191 187
79 33 33 33
SfoI GGC'GCC
Cuts at: 2864 3112 2864
Size: 248 5184
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
SmaI CCC'GGG
Cuts at: 2454 2454
Size: 5432
SmlI C'TyrA_G
Cuts at: 743 2587 3502 3994 4256 743
Size: 1844 915 492 262 1919
Fragments arranged by size:
1919 1844 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5432
SphI G_CATG'C
Cuts at: 2485 2985 2485
Size: 500 4932
StuI AGG'CCT
Cuts at: 1115 1115
Size: 5432
StyI C'CwwG_G
Cuts at: 1454 2334 2566 2920 1454
Size: 880 232 354 3966
Fragments arranged by size:
3966 880 354 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2197 3015 3148 3607 3816 2197
Size: 818 133 459 209 3813
Fragments arranged by size:
3813 818 459 209 133
TatI w'GTAC_w
Cuts at: 690 913 995 4947 690
Size: 223 82 3952 1175
Fragments arranged by size:
3952 1175 223 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1450 1482 2315 2347 4949 4981 1450
Size: 32 833 32 2602 32 1901
Fragments arranged by size:
2602 1901 833 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 139 3229 139
Size: 3090 2342
XbaI T'CTAG_A
Cuts at: 2463 2463
Size: 5432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2151 2927 2151
Size: 776 4656
XmaI C'CCGG_G
Cuts at: 2452 2452
Size: 5432
XmnI GAAnn'nnTTC
Cuts at: 872 872
Size: 5432
ZraI GAC'GTC
Cuts at: 1461 1461
Size: 5432
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BglI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI PacI PasI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI