Restriction map for
pF1KB8575
(Circular) (Six-base) MAPSORT of: pF1KB8575.seq Check: 446 from: 1 to: 4130
pF1KB8575 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 515 1168 515
Size: 653 3477
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1955 2387 2740 3402 949
Size: 1006 432 353 662 1677
Fragments arranged by size:
1677 1006 662 432 353
AflIII A'CryG_T
Cuts at: 1016 1482 3282 3563 1016
Size: 466 1800 281 1583
Fragments arranged by size:
1800 1583 466 281
AlwNI CAG_nnn'CTG
Cuts at: 573 886 1435 2873 573
Size: 313 549 1438 1830
Fragments arranged by size:
1830 1438 549 313
ApaLI G'TGCA_C
Cuts at: 175 496 2968 175
Size: 321 2472 1337
Fragments arranged by size:
2472 1337 321
ApoI r'AATT_y
Cuts at: 1134 3483 3739 1134
Size: 2349 256 1525
Fragments arranged by size:
2349 1525 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 863 1150 863
Size: 287 3843
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 220 1146 1560 1808 1843 220
Size: 926 414 248 35 2507
Fragments arranged by size:
2507 926 414 248 35
BanII G_rGCy'C
Cuts at: 1144 2174 1144
Size: 1030 3100
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbsI GAAGACnn'nnnn_
Cuts at: 682 682
Size: 4130
BciVI GTATCCnnnnn_n'
Cuts at: 2028 3084 4130 2028
Size: 1056 1046 2028
Fragments arranged by size:
2028 1056 1046
BclI T'GATC_A
Cuts at: 531 1650 531
Size: 1119 3011
BglI GCCn_nnn'nGGC
Cuts at: 778 778
Size: 4130
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 1075 1242 1075
Size: 167 3963
Bme1580I G_kGCm'C
Cuts at: 179 225 500 1755 1848 2972 179
Size: 46 275 1255 93 1124 1337
Fragments arranged by size:
1337 1255 1124 275 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 1342 1423 1758 3902 1342
Size: 81 335 2144 1570
Fragments arranged by size:
2144 1570 335 81
BmtI G_CTAG'C
Cuts at: 1066 1066
Size: 4130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 289
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2185 2899 3197 1306
Size: 879 714 298 2239
Fragments arranged by size:
2239 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 545 1644 3681 6
Size: 539 1099 2037 455
Fragments arranged by size:
2037 1099 539 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1319 1840 2518 2929 3076 3350 1319
Size: 521 678 411 147 274 2099
Fragments arranged by size:
2099 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 113 143 562 592 113
Size: 30 419 30 3651
Fragments arranged by size:
3651 419 30 30
BseYI C'CCAG_C
Cuts at: 1555 2313 2978 3386 1555
Size: 758 665 408 2299
Fragments arranged by size:
2299 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 4130
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2948 104
Size: 1568 46 1230 1286
Fragments arranged by size:
1568 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 179 500 1144 1922 2112 2972 179
Size: 321 644 778 190 860 1337
Fragments arranged by size:
1337 860 778 644 321 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1099 1099
Size: 4130
Bsp1286I G_dGCh'C
Cuts at: 179 225 500 1144 1755 1848 1922 2112 2174
Size: 46 275 644 611 93 74 190 62
Cuts at: 2174 2972 179
Size: 798 1337
Fragments arranged by size:
1337 798 644 611 275 190 93 74
62 46
BspHI T'CATG_A
Cuts at: 2562 2562
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 738 2422 2476 3960 4125 50
Size: 688 1684 54 1484 165 55
Fragments arranged by size:
1684 1484 688 165 55 54
BsrDI GCAATG_nn'
Cuts at: 689 971 2042 689
Size: 282 1071 2777
Fragments arranged by size:
2777 1071 282
BsrFI r'CCGG_y
Cuts at: 2128 2309 2128
Size: 181 3949
BssSI C'ACGA_G
Cuts at: 2401 3109 2401
Size: 708 3422
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 464 1155 1622 1645 1980 2226 2532 2544
Size: 463 691 467 23 335 246 306 12
Cuts at: 2544 2630 3682 1
Size: 86 1052 449
Fragments arranged by size:
1052 691 467 463 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 581 107
Size: 474 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 410 498 896 1044 1373 410
Size: 88 398 148 329 3167
Fragments arranged by size:
3167 398 329 148 88
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 3180 1836
Size: 1344 2786
EaeI y'GGCC_r
Cuts at: 1109 1715 1889 2280 2307 3652 1109
Size: 606 174 391 27 1345 1587
Fragments arranged by size:
1587 1345 606 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 169 182 2153 2363 169
Size: 13 1971 210 1936
Fragments arranged by size:
1971 1936 210 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 3080 3226 3940 2338
Size: 742 146 714 2528
Fragments arranged by size:
2528 742 714 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 949 1955 2387 2740 3402 289
Size: 660 1006 432 353 662 1017
Fragments arranged by size:
1017 1006 662 660 432 353
EcoO109I rG'GnC_Cy
Cuts at: 447 570 1269 447
Size: 123 699 3308
Fragments arranged by size:
3308 699 123
EcoRI G'AATT_C
Cuts at: 1134 1134
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1011 1095 2149 1011
Size: 84 1054 2992
Fragments arranged by size:
2992 1054 84
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 751 1564 1812 3042 3813 751
Size: 813 248 1230 771 1068
Fragments arranged by size:
1230 1068 813 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 1972 2002 2004 2034 562
Size: 32 1378 30 2 30 2658
Fragments arranged by size:
2658 1378 32 30 30 2
HincII GTy'rAC
Cuts at: 1169 1169
Size: 4130
HindIII A'AGCT_T
Cuts at: 682 1077 682
Size: 395 3735
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1004 2889 3073 1004
Size: 1885 184 2061
Fragments arranged by size:
2061 1885 184
MscI TGG'CCA
Cuts at: 1891 1891
Size: 4130
MslI CAynn'nnrTG
Cuts at: 901 1021 2119 2246 901
Size: 120 1098 127 2785
Fragments arranged by size:
2785 1098 127 120
MspA1I CmG'CkG
Cuts at: 172 242 954 1241 1555 1915 2699 2944 172
Size: 70 712 287 314 360 784 245 1358
Fragments arranged by size:
1358 784 712 360 314 287 245 70
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NcoI C'CATG_G
Cuts at: 107 581 107
Size: 474 3656
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NheI G'CTAG_C
Cuts at: 1062 1062
Size: 4130
NspI r_CATG'y
Cuts at: 1020 1183 1683 3286 1020
Size: 163 500 1603 1864
Fragments arranged by size:
1864 1603 500 163
PasI CC'CwG_GG
Cuts at: 589 589
Size: 4130
PciI A'CATG_T
Cuts at: 1016 3282 1016
Size: 2266 1864
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 4098
PpuMI rG'GwC_Cy
Cuts at: 570 570
Size: 4130
PstI C_TGCA'G
Cuts at: 493 953 1177 1862 493
Size: 460 224 685 2761
Fragments arranged by size:
2761 685 460 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 172 954 1555 1915 172
Size: 782 601 360 2387
Fragments arranged by size:
2387 782 601 360
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 489 949 1173 1858 2826 3017 33
Size: 456 460 224 685 968 191 1146
Fragments arranged by size:
1146 968 685 460 456 224 191
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 865 1152 865
Size: 287 3843
SmlI C'TyrA_G
Cuts at: 1285 2200 2914 3176 1285
Size: 915 714 262 2239
Fragments arranged by size:
2239 915 714 262
SpeI A'CTAG_T
Cuts at: 67 340 67
Size: 273 3857
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
StyI C'CwwG_G
Cuts at: 107 581 1100 1264 1618 107
Size: 474 519 164 354 2619
Fragments arranged by size:
2619 519 474 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1713 1846 2305 2514 1713
Size: 133 459 209 3329
Fragments arranged by size:
3329 459 209 133
TatI w'GTAC_w
Cuts at: 962 3645 962
Size: 2683 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 113 145 330 362 3647 3679 113
Size: 32 185 32 3285 32 564
Fragments arranged by size:
3285 564 185 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1625 114
Size: 1511 2619
XmaI C'CCGG_G
Cuts at: 863 1150 863
Size: 287 3843
XmnI GAAnn'nnTTC
Cuts at: 484 484
Size: 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BmgBI Bpu10I BseRI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI