Restriction map for
pF1KB8569
(Circular) (Six-base) MAPSORT of: pF1KB8569.seq Check: 2364 from: 1 to: 5426
pF1KB8569 5426 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2464 2464
Size: 5426
Acc65I G'GTAC_C
Cuts at: 2442 2442
Size: 5426
AclI AA'CG_TT
Cuts at: 5266 5266
Size: 5426
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 501 961 1483 1834 1906 3251 3683 4036
Size: 301 460 522 351 72 1345 432 353
Cuts at: 4036 4698 200
Size: 662 928
Fragments arranged by size:
1345 928 662 522 460 432 353 351
301 72
AfeI AGC'GCT
Cuts at: 1093 1093
Size: 5426
AflII C'TTAA_G
Cuts at: 1891 1891
Size: 5426
AflIII A'CryG_T
Cuts at: 2778 4578 4859 2778
Size: 1800 281 3345
Fragments arranged by size:
3345 1800 281
AgeI A'CCGG_T
Cuts at: 2189 2189
Size: 5426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 787 1427 2135 787
Size: 640 708 4078
Fragments arranged by size:
4078 708 640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1543 1575 1543
Size: 32 5394
AlwNI CAG_nnn'CTG
Cuts at: 139 178 236 401 460 694 967 2011 2731
Size: 39 58 165 59 234 273 1044 720
Cuts at: 2731 4169 139
Size: 1438 1396
Fragments arranged by size:
1438 1396 1044 720 273 234 165 59
58 39
ApaI G_GGCC'C
Cuts at: 672 871 672
Size: 199 5227
ApaLI G'TGCA_C
Cuts at: 4264 4264
Size: 5426
ApoI r'AATT_y
Cuts at: 1729 2430 4779 5035 1729
Size: 701 2349 256 2120
Fragments arranged by size:
2349 2120 701 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5426
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5426
AvaI C'yCGr_G
Cuts at: 490 1078 1176 2446 490
Size: 588 98 1270 3470
Fragments arranged by size:
3470 1270 588 98
BamHI G'GATC_C
Cuts at: 1322 1955 2451 1322
Size: 633 496 4297
Fragments arranged by size:
4297 633 496
BanI G'GyrC_C
Cuts at: 262 562 1025 1926 2442 2856 3104 3139 262
Size: 300 463 901 516 414 248 35 2549
Fragments arranged by size:
2549 901 516 463 414 300 248 35
BanII G_rGCy'C
Cuts at: 672 871 974 2275 2440 3470 672
Size: 199 103 1301 165 1030 2628
Fragments arranged by size:
2628 1301 1030 199 165 103
BbeI G_GCGC'C
Cuts at: 2860 3108 2860
Size: 248 5178
BbsI GAAGACnn'nnnn_
Cuts at: 1471 1678 2347 1471
Size: 207 669 4550
Fragments arranged by size:
4550 669 207
BbvCI CC'TCA_GC
Cuts at: 319 461 470 1525 2362 319
Size: 142 9 1055 837 3383
Fragments arranged by size:
3383 1055 837 142 9
BciVI GTATCCnnnnn_n'
Cuts at: 3324 4380 5426 3324
Size: 1056 1046 3324
Fragments arranged by size:
3324 1056 1046
BclI T'GATC_A
Cuts at: 2946 2946
Size: 5426
BglI GCCn_nnn'nGGC
Cuts at: 866 1852 2305 866
Size: 986 453 3987
Fragments arranged by size:
3987 986 453
BglII A'GATC_T
Cuts at: 2941 4978 2941
Size: 2037 3389
BlpI GC'TnA_GC
Cuts at: 598 973 2538 598
Size: 375 1565 3486
Fragments arranged by size:
3486 1565 375
Bme1580I G_kGCm'C
Cuts at: 251 265 565 672 871 1344 3051 3144 4268
Size: 14 300 107 199 473 1707 93 1124
Cuts at: 4268 251
Size: 1409
Fragments arranged by size:
1707 1409 1124 473 300 199 107 93
14
BmrI ACTGGGnnnn_n'
Cuts at: 561 2143 2417 2638 2719 3054 5198 561
Size: 1582 274 221 81 335 2144 789
Fragments arranged by size:
2144 1582 789 335 274 221 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3793 3793
Size: 5426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 562 771 1926 2305 423
Size: 139 209 1155 379 3544
Fragments arranged by size:
3544 1155 379 209 139
Bpu10I CC'TnA_GC
Cuts at: 319 461 470 683 811 1525 2362 319
Size: 142 9 213 128 714 837 3383
Fragments arranged by size:
3383 837 714 213 142 128 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2602 3481 4195 4493 2602
Size: 879 714 298 3535
Fragments arranged by size:
3535 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2096 2227 5130 2096
Size: 131 2903 2392
Fragments arranged by size:
2903 2392 131
BsaAI yAC'GTr
Cuts at: 2779 2779
Size: 5426
BsaBI GATnn'nnATC
Cuts at: 6 2940 4977 6
Size: 2934 2037 455
Fragments arranged by size:
2934 2037 455
BsaHI Gr'CG_yC
Cuts at: 1658 1702 2857 3105 3807 5303 1658
Size: 44 1155 248 702 1496 1781
Fragments arranged by size:
1781 1496 1155 702 248 44
BsaWI w'CCGG_w
Cuts at: 197 2189 2336 2615 3136 3814 4225 4372 4646
Size: 1992 147 279 521 678 411 147 274
Cuts at: 4646 197
Size: 977
Fragments arranged by size:
1992 977 678 521 411 279 274 147
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2176 2206 2176
Size: 30 5396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 199 910 2092 2197 127
Size: 72 711 1182 105 3356
Fragments arranged by size:
3356 1182 711 105 72
BseYI C'CCAG_C
Cuts at: 267 474 694 759 934 1113 2004 2851 3609
Size: 207 220 65 175 179 891 847 758
Cuts at: 3609 4274 4682 267
Size: 665 408 1011
Fragments arranged by size:
1011 891 847 758 665 408 220 207
179 175 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1025 1351 2040 1025
Size: 326 689 4411
Fragments arranged by size:
4411 689 326
BsiEI CG_ry'CG
Cuts at: 104 2968 3014 4244 104
Size: 2864 46 1230 1286
Fragments arranged by size:
2864 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 974 1200 1745 2204 2440 3218 3408 4268 974
Size: 226 545 459 236 778 190 860 2132
Fragments arranged by size:
2132 860 778 545 459 236 226 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1072 1072
Size: 5426
Bsp1286I G_dGCh'C
Cuts at: 251 265 565 672 871 974 1200 1344 1745
Size: 14 300 107 199 103 226 144 401
Cuts at: 1745 2204 2275 2440 3051 3144 3218 3408 3470
Size: 459 71 165 611 93 74 190 62
Cuts at: 3470 4268 251
Size: 798 1409
Fragments arranged by size:
1409 798 611 459 401 300 226 199
190 165 144 107 103 93 74 71
62 14
BspEI T'CCGG_A
Cuts at: 197 2336 197
Size: 2139 3287
BspHI T'CATG_A
Cuts at: 2094 3858 2094
Size: 1764 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1373 1466 1772 2476 2690 2992 3373 1373
Size: 93 306 704 214 302 381 3426
Fragments arranged by size:
3426 704 381 306 302 214 93
BsrBI CCG'CTC
Cuts at: 50 797 1254 2289 3718 3772 5256 5421 50
Size: 747 457 1035 1429 54 1484 165 55
Fragments arranged by size:
1484 1429 1035 747 457 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1409 3338 1409
Size: 1929 3497
BsrFI r'CCGG_y
Cuts at: 1277 2189 3424 3605 1277
Size: 912 1235 181 3098
Fragments arranged by size:
3098 1235 912 181
BssSI C'ACGA_G
Cuts at: 250 3697 4405 250
Size: 3447 708 1271
Fragments arranged by size:
3447 1271 708
BstAPI GCAn_nnn'nTGC
Cuts at: 236 2803 236
Size: 2567 2859
BstBI TT'CG_AA
Cuts at: 3787 3787
Size: 5426
BstEII G'GTnAC_C
Cuts at: 1807 1807
Size: 5426
BstXI CCAn_nnnn'nTGG
Cuts at: 856 1381 2380 856
Size: 525 999 3902
Fragments arranged by size:
3902 999 525
BstYI r'GATC_y
Cuts at: 1 1322 1955 2451 2918 2941 3276 3522 3828
Size: 1321 633 496 467 23 335 246 306
Cuts at: 3828 3840 3926 4978 1
Size: 12 86 1052 449
Fragments arranged by size:
1321 1052 633 496 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 140 863 1960 140
Size: 723 1097 3606
Fragments arranged by size:
3606 1097 723
BtgI C'CryG_G
Cuts at: 107 312 1707 107
Size: 205 1395 3826
Fragments arranged by size:
3826 1395 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 164 3376 3557 3720 164
Size: 3212 181 163 1870
Fragments arranged by size:
3212 1870 181 163
BtsI GCAGTG_nn'
Cuts at: 140 1745 2669 140
Size: 1605 924 2897
Fragments arranged by size:
2897 1605 924
DraI TTT'AAA
Cuts at: 2425 2425
Size: 5426
DrdI GACnn_nn'nnGTC
Cuts at: 625 3132 4476 625
Size: 2507 1344 1575
Fragments arranged by size:
2507 1575 1344
EaeI y'GGCC_r
Cuts at: 209 830 1016 1145 1371 1437 1844 2129 2306
Size: 621 186 129 226 66 407 285 177
Cuts at: 2306 3011 3185 3576 3603 4948 209
Size: 705 174 391 27 1345 687
Fragments arranged by size:
1345 705 687 621 407 391 285 226
186 177 174 129 66 27
EagI C'GGCC_G
Cuts at: 3011 3011
Size: 5426
EarI CTCTTCn'nnn_
Cuts at: 176 1924 3449 3659 176
Size: 1748 1525 210 1943
Fragments arranged by size:
1943 1748 1525 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3634 4376 4522 5236 3634
Size: 742 146 714 3824
Fragments arranged by size:
3824 742 714 146
EcoICRI GAG'CTC
Cuts at: 972 2438 972
Size: 1466 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 423 501 562 771 961 1483 1834 1906
Size: 223 78 61 209 190 522 351 72
Cuts at: 1906 1926 2305 3251 3683 4036 4698 200
Size: 20 379 946 432 353 662 928
Fragments arranged by size:
946 928 662 522 432 379 353 351
223 209 190 78 72 61 20
EcoNI CCTnn'n_nnAGG
Cuts at: 143 686 1768 1921 2071 2365 143
Size: 543 1082 153 150 294 3204
Fragments arranged by size:
3204 1082 543 294 153 150
EcoO109I rG'GnC_Cy
Cuts at: 219 294 398 465 639 867 1257 1853 2565
Size: 75 104 67 174 228 390 596 712
Cuts at: 2565 219
Size: 3080
Fragments arranged by size:
3080 712 596 390 228 174 104 75
67
EcoRI G'AATT_C
Cuts at: 2430 2430
Size: 5426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 5426
FspI TGC'GCA
Cuts at: 3207 3207
Size: 5426
HaeII r_GCGC'y
Cuts at: 524 780 1095 1633 2860 3108 4338 5109 524
Size: 256 315 538 1227 248 1230 771 841
Fragments arranged by size:
1230 1227 841 771 538 315 256 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 937 949 969 981 1383 1415 3268 3298 3300
Size: 12 20 12 402 32 1853 30 2
Cuts at: 3300 3330 937
Size: 30 3033
Fragments arranged by size:
3033 1853 402 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 2465 2465
Size: 5426
HindIII A'AGCT_T
Cuts at: 1888 1888
Size: 5426
KasI G'GCGC_C
Cuts at: 2856 3104 2856
Size: 248 5178
KpnI G_GTAC'C
Cuts at: 2446 2446
Size: 5426
MluI A'CGCG_T
Cuts at: 4859 4859
Size: 5426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 971 1901 4185 4369 461
Size: 510 930 2284 184 1518
Fragments arranged by size:
2284 1518 930 510 184
MscI TGG'CCA
Cuts at: 211 832 1018 1439 1846 2308 3187 211
Size: 621 186 421 407 462 879 2450
Fragments arranged by size:
2450 879 621 462 421 407 186
MslI CAynn'nnrTG
Cuts at: 832 854 1828 3415 3542 832
Size: 22 974 1587 127 2716
Fragments arranged by size:
2716 1587 974 127 22
MspA1I CmG'CkG
Cuts at: 271 388 474 623 666 742 1113 1227 1428
Size: 117 86 149 43 76 371 114 201
Cuts at: 1428 2011 2115 2537 2851 3211 3995 4240 271
Size: 583 104 422 314 360 784 245 1457
Fragments arranged by size:
1457 784 583 422 371 360 314 245
201 149 117 114 104 86 76 43
NaeI GCC'GGC
Cuts at: 3607 3607
Size: 5426
NarI GG'CG_CC
Cuts at: 2857 3105 2857
Size: 248 5178
NcoI C'CATG_G
Cuts at: 107 312 107
Size: 205 5221
NgoMIV G'CCGG_C
Cuts at: 3605 3605
Size: 5426
NspI r_CATG'y
Cuts at: 2479 2979 4582 2479
Size: 500 1603 3323
Fragments arranged by size:
3323 1603 500
PasI CC'CwG_GG
Cuts at: 244 354 871 244
Size: 110 517 4799
Fragments arranged by size:
4799 517 110
PciI A'CATG_T
Cuts at: 4578 4578
Size: 5426
PflMI CCAn_nnn'nTGG
Cuts at: 401 694 2250 401
Size: 293 1556 3577
Fragments arranged by size:
3577 1556 293
PfoI T'CCnGG_A
Cuts at: 439 1592 1613 1951 439
Size: 1153 21 338 3914
Fragments arranged by size:
3914 1153 338 21
PmeI GTTT'AAAC
Cuts at: 2425 2425
Size: 5426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 5394
PpuMI rG'GwC_Cy
Cuts at: 294 465 1257 294
Size: 171 792 4463
Fragments arranged by size:
4463 792 171
PshAI GACnn'nnGTC
Cuts at: 167 167
Size: 5426
PspOMI G'GGCC_C
Cuts at: 668 867 668
Size: 199 5227
PspXI vC'TCGA_Gb
Cuts at: 1078 1078
Size: 5426
PstI C_TGCA'G
Cuts at: 135 241 1769 2473 3158 135
Size: 106 1528 704 685 2403
Fragments arranged by size:
2403 1528 704 685 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5426
PvuII CAG'CTG
Cuts at: 474 623 742 1113 1428 2115 2851 3211 474
Size: 149 119 371 315 687 736 360 2689
Fragments arranged by size:
2689 736 687 371 360 315 149 119
RsrII CG'GwC_CG
Cuts at: 3621 3621
Size: 5426
SacI G_AGCT'C
Cuts at: 974 2440 974
Size: 1466 3960
SalI G'TCGA_C
Cuts at: 2463 2463
Size: 5426
SapI GCTCTTCn'nnn_
Cuts at: 3449 3659 3449
Size: 210 5216
SbfI CC_TGCA'GG
Cuts at: 2473 2473
Size: 5426
ScaI AGT'ACT
Cuts at: 4943 4943
Size: 5426
SexAI A'CCwGG_T
Cuts at: 1970 1970
Size: 5426
SfcI C'TryA_G
Cuts at: 33 131 237 1765 1863 2469 3154 4122 4313
Size: 98 106 1528 98 606 685 968 191
Cuts at: 4313 33
Size: 1146
Fragments arranged by size:
1528 1146 968 685 606 191 106 98
98
SfiI GGCCn_nnn'nGGCC
Cuts at: 866 1852 2305 866
Size: 986 453 3987
Fragments arranged by size:
3987 986 453
SfoI GGC'GCC
Cuts at: 2858 3106 2858
Size: 248 5178
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5426
SmaI CCC'GGG
Cuts at: 492 2448 492
Size: 1956 3470
SmlI C'TyrA_G
Cuts at: 1078 1891 2581 3496 4210 4472 1078
Size: 813 690 915 714 262 2032
Fragments arranged by size:
2032 915 813 714 690 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5426
SphI G_CATG'C
Cuts at: 2479 2979 2479
Size: 500 4926
StuI AGG'CCT
Cuts at: 1219 1785 1219
Size: 566 4860
StyI C'CwwG_G
Cuts at: 107 312 324 2171 2560 2914 107
Size: 205 12 1847 389 354 2619
Fragments arranged by size:
2619 1847 389 354 205 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 1678 2162 3009 3142 3601 3810 580
Size: 1098 484 847 133 459 209 2196
Fragments arranged by size:
2196 1098 847 484 459 209 133
TatI w'GTAC_w
Cuts at: 569 1778 4941 569
Size: 1209 3163 1054
Fragments arranged by size:
3163 1209 1054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 641 673 1692 1724 4943 4975 198
Size: 32 411 32 1019 32 3219 32 649
Fragments arranged by size:
3219 1019 649 411 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3223 3223
Size: 5426
XbaI T'CTAG_A
Cuts at: 2457 2457
Size: 5426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1432 2921 1432
Size: 1489 3937
XhoI C'TCGA_G
Cuts at: 1078 1078
Size: 5426
XmaI C'CCGG_G
Cuts at: 490 2446 490
Size: 1956 3470
XmnI GAAnn'nnTTC
Cuts at: 1219 1729 2029 1219
Size: 510 300 4616
Fragments arranged by size:
4616 510 300
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI SwaI
ZraI