Restriction map for
pF1KB8566
(Circular) (Six-base) MAPSORT of: pF1KB8566.seq Check: 9896 from: 1 to: 3875
pF1KB8566 3875 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 832 3715 832
Size: 2883 992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2485 3147 1700
Size: 432 353 662 2428
Fragments arranged by size:
2428 662 432 353
AflIII A'CryG_T
Cuts at: 612 1227 3027 3308 612
Size: 615 1800 281 1179
Fragments arranged by size:
1800 1179 615 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 194 194
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 1180 2618 1180
Size: 1438 2437
ApaLI G'TGCA_C
Cuts at: 2713 2713
Size: 3875
ApoI r'AATT_y
Cuts at: 145 879 3228 3484 145
Size: 734 2349 256 536
Fragments arranged by size:
2349 734 536 256
AseI AT'TA_AT
Cuts at: 20 826 20
Size: 806 3069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 342 375 342
Size: 33 3842
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 889 1919 889
Size: 1030 2845
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BbsI GAAGACnn'nnnn_
Cuts at: 671 671
Size: 3875
BbvCI CC'TCA_GC
Cuts at: 139 139
Size: 3875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 589 623 589
Size: 34 3841
BciVI GTATCCnnnnn_n'
Cuts at: 303 580 1773 2829 3875 303
Size: 277 1193 1056 1046 303
Fragments arranged by size:
1193 1056 1046 303 277
BclI T'GATC_A
Cuts at: 194 1395 194
Size: 1201 2674
BglII A'GATC_T
Cuts at: 492 1390 3427 492
Size: 898 2037 940
Fragments arranged by size:
2037 940 898
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2717 1500
Size: 93 1124 2658
Fragments arranged by size:
2658 1124 93
BmgBI CAC'GTC
Cuts at: 613 613
Size: 3875
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
Bpu10I CC'TnA_GC
Cuts at: 139 139
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2644 2942 1051
Size: 879 714 298 1984
Fragments arranged by size:
1984 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2674 2821 3095 1064
Size: 521 678 411 147 274 1844
Fragments arranged by size:
1844 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 3875
BseYI C'CCAG_C
Cuts at: 1300 2058 2723 3131 1300
Size: 758 665 408 2044
Fragments arranged by size:
2044 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2693 104
Size: 1313 46 1230 1286
Fragments arranged by size:
1313 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 889 1667 1857 2717 889
Size: 778 190 860 2047
Fragments arranged by size:
2047 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 334 334
Size: 3875
Bsp1286I G_dGCh'C
Cuts at: 889 1500 1593 1667 1857 1919 2717 889
Size: 611 93 74 190 62 798 2047
Fragments arranged by size:
2047 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2307 2307
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 847 1787 847
Size: 940 2935
BsrFI r'CCGG_y
Cuts at: 1873 2054 1873
Size: 181 3694
BssSI C'ACGA_G
Cuts at: 861 2146 2854 861
Size: 1285 708 1882
Fragments arranged by size:
1882 1285 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 492 900 1367 1390 1725 1971 2277 2289
Size: 491 408 467 23 335 246 306 12
Cuts at: 2289 2375 3427 1
Size: 86 1052 449
Fragments arranged by size:
1052 491 467 449 408 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 695 695
Size: 3875
BtgI C'CryG_G
Cuts at: 113 113
Size: 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 798 1118 798
Size: 320 3555
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2925 1581
Size: 1344 2531
EaeI y'GGCC_r
Cuts at: 1460 1634 2025 2052 3397 1460
Size: 174 391 27 1345 1938
Fragments arranged by size:
1938 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 2083 2825 2971 3685 134
Size: 1949 742 146 714 324
Fragments arranged by size:
1949 742 714 324 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2485 3147 1700
Size: 432 353 662 2428
Fragments arranged by size:
2428 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1014 1014
Size: 3875
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
EcoRV GAT'ATC
Cuts at: 390 390
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 621 1894 621
Size: 1273 2602
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2787 3558 1309
Size: 248 1230 771 1626
Fragments arranged by size:
1626 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 1717 1747 1749 1779 244
Size: 32 1441 30 2 30 2340
Fragments arranged by size:
2340 1441 32 30 30 2
HincII GTy'rAC
Cuts at: 402 914 402
Size: 512 3363
HpaI GTT'AAC
Cuts at: 402 402
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2634 2818 2634
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 115 138 373 986 1300 1660 2444 2689 115
Size: 23 235 613 314 360 784 245 1301
Fragments arranged by size:
1301 784 613 360 314 245 235 23
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 928 1428 3031 928
Size: 500 1603 1772
Fragments arranged by size:
1772 1603 500
PciI A'CATG_T
Cuts at: 3027 3027
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 2321
Size: 32 3843
PstI C_TGCA'G
Cuts at: 372 793 922 1607 372
Size: 421 129 685 2640
Fragments arranged by size:
2640 685 421 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 373 1300 1660 373
Size: 927 360 2588
Fragments arranged by size:
2588 927 360
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SacII CC_GC'GG
Cuts at: 116 116
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 368 374 789 918 1603 2571 2762 33
Size: 335 6 415 129 685 968 191 1146
Fragments arranged by size:
1146 968 685 415 335 191 129 6
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 1030 1945 2659 2921 1030
Size: 915 714 262 1984
Fragments arranged by size:
1984 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
SspI AAT'ATT
Cuts at: 801 801
Size: 3875
StyI C'CwwG_G
Cuts at: 1009 1363 1009
Size: 354 3521
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 272 3390 272
Size: 3118 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
XmnI GAAnn'nnTTC
Cuts at: 814 814
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BfrBI BglI BmtI BpmI BsaXI
BsgI BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI