Restriction map for
pF1KB8561
(Circular) (Six-base) MAPSORT of: pF1KB8561.seq Check: 5225 from: 1 to: 6014
pF1KB8561 6014 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1006 3009 1006
Size: 2003 4011
AatII G_ACGT'C
Cuts at: 1931 1931
Size: 6014
AccI GT'mk_AC
Cuts at: 1436 3052 1436
Size: 1616 4398
Acc65I G'GTAC_C
Cuts at: 192 3030 192
Size: 2838 3176
AclI AA'CG_TT
Cuts at: 5854 5854
Size: 6014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 409 559 1051 1690 3839 4271 5018 5286
Size: 224 150 492 639 2149 432 747 268
Cuts at: 5286 185
Size: 913
Fragments arranged by size:
2149 913 747 639 492 432 268 224
150
AfeI AGC'GCT
Cuts at: 601 601
Size: 6014
AflIII A'CryG_T
Cuts at: 3366 4470 5447 3366
Size: 1104 977 3933
Fragments arranged by size:
3933 1104 977
AgeI A'CCGG_T
Cuts at: 2061 4402 2061
Size: 2341 3673
AhdI GACnn_n'nnGTC
Cuts at: 699 699
Size: 6014
AleI CACnn'nnGTG
Cuts at: 1684 1684
Size: 6014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 649
Size: 6014
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1648 1680 1648
Size: 32 5982
AlwNI CAG_nnn'CTG
Cuts at: 1129 2367 2814 2910 3319 4886 1129
Size: 1238 447 96 409 1567 2257
Fragments arranged by size:
2257 1567 1238 447 409 96
ApaI G_GGCC'C
Cuts at: 353 2546 353
Size: 2193 3821
ApaLI G'TGCA_C
Cuts at: 1744 4784 1744
Size: 3040 2974
ApoI r'AATT_y
Cuts at: 3018 5367 5623 3018
Size: 2349 256 3409
Fragments arranged by size:
3409 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6014
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
AvaI C'yCGr_G
Cuts at: 2559 2689 3034 2559
Size: 130 345 5539
Fragments arranged by size:
5539 345 130
AvrII C'CTAG_G
Cuts at: 1204 1204
Size: 6014
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 207 240 207
Size: 33 5981
BamHI G'GATC_C
Cuts at: 962 3039 962
Size: 2077 3937
BanI G'GyrC_C
Cuts at: 192 1034 1084 2803 3030 3444 3692 3727 192
Size: 842 50 1719 227 414 248 35 2479
Fragments arranged by size:
2479 1719 842 414 248 227 50 35
BanII G_rGCy'C
Cuts at: 128 182 353 656 716 1598 2228 2546 2663
Size: 54 171 303 60 882 630 318 117
Cuts at: 2663 2675 2840 3028 4058 128
Size: 12 165 188 1030 2084
Fragments arranged by size:
2084 1030 882 630 318 303 188 171
165 117 60 54 12
BbeI G_GCGC'C
Cuts at: 3448 3696 3448
Size: 248 5766
BbsI GAAGACnn'nnnn_
Cuts at: 527 527
Size: 6014
BbvCI CC'TCA_GC
Cuts at: 382 382
Size: 6014
BciVI GTATCCnnnnn_n'
Cuts at: 235 1631 3912 4673 6014 235
Size: 1396 2281 761 1341 235
Fragments arranged by size:
2281 1396 1341 761 235
BclI T'GATC_A
Cuts at: 1535 3534 1535
Size: 1999 4015
BglI GCCn_nnn'nGGC
Cuts at: 1093 2562 2989 1093
Size: 1469 427 4118
Fragments arranged by size:
4118 1469 427
BglII A'GATC_T
Cuts at: 3529 5566 3529
Size: 2037 3977
BlpI GC'TnA_GC
Cuts at: 334 3126 334
Size: 2792 3222
Bme1580I G_kGCm'C
Cuts at: 151 353 1089 1748 2546 2806 3639 3732 4788
Size: 202 736 659 798 260 833 93 1056
Cuts at: 4788 151
Size: 1377
Fragments arranged by size:
1377 1056 833 798 736 659 260 202
93
BmgBI CAC'GTC
Cuts at: 219 219
Size: 6014
BmrI ACTGGGnnnn_n'
Cuts at: 2099 3226 3307 3642 5786 2099
Size: 1127 81 335 2144 2327
Fragments arranged by size:
2327 2144 1127 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2213 4381 2213
Size: 2168 3846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1066 1279 1669 1747 2014 2041 2551 2677
Size: 396 213 390 78 267 27 510 126
Cuts at: 2677 670
Size: 4007
Fragments arranged by size:
4007 510 396 390 267 213 126 78
27
Bpu10I CC'TnA_GC
Cuts at: 130 382 655 829 1963 2446 130
Size: 252 273 174 1134 483 3698
Fragments arranged by size:
3698 1134 483 273 252 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 2647 3190 4069 4561 4859 214
Size: 2433 543 879 492 298 1369
Fragments arranged by size:
2433 1369 879 543 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 889 2024 5718 889
Size: 1135 3694 1185
Fragments arranged by size:
3694 1185 1135
BsaAI yAC'GTr
Cuts at: 474 2700 3367 474
Size: 2226 667 3121
Fragments arranged by size:
3121 2226 667
BsaBI GATnn'nnATC
Cuts at: 6 1579 3528 5565 6
Size: 1573 1949 2037 455
Fragments arranged by size:
2037 1949 1573 455
BsaHI Gr'CG_yC
Cuts at: 1928 2506 2939 3445 3693 4395 5891 1928
Size: 578 433 506 248 702 1496 2051
Fragments arranged by size:
2051 1496 702 578 506 433 248
BsaWI w'CCGG_w
Cuts at: 359 581 788 1763 2061 2603 3203 3724 4402
Size: 222 207 975 298 542 600 521 678
Cuts at: 4402 4676 4823 5234 359
Size: 274 147 411 1139
Fragments arranged by size:
1139 975 678 600 542 521 411 298
274 222 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 826 856 1648 1678 2446 2476 826
Size: 30 792 30 768 30 4364
Fragments arranged by size:
4364 792 768 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 850 853 1063 1606 2338 2470 2905 850
Size: 3 210 543 732 132 435 3959
Fragments arranged by size:
3959 732 543 435 210 132 3
BseYI C'CCAG_C
Cuts at: 639 1071 1635 1800 2090 2257 2341 2360 2565
Size: 432 564 165 290 167 84 19 205
Cuts at: 2565 3439 4197 4774 5270 639
Size: 874 758 577 496 1383
Fragments arranged by size:
1383 874 758 577 564 496 432 290
205 167 165 84 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 454 469 1479 2157 406
Size: 48 15 1010 678 4263
Fragments arranged by size:
4263 1010 678 48 15
BsiEI CG_ry'CG
Cuts at: 104 3556 3602 4810 104
Size: 3452 46 1208 1308
Fragments arranged by size:
3452 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 656 716 1181 1679 1748 2663 2675 2840 3028
Size: 60 465 498 69 915 12 165 188
Cuts at: 3028 3806 3996 4788 656
Size: 778 190 792 1882
Fragments arranged by size:
1882 915 792 778 498 465 190 188
165 69 60 12
BsmI GAATG_Cn'
Cuts at: 2199 2199
Size: 6014
BsmBI CGTCTCn'nnnn_
Cuts at: 1870 1870
Size: 6014
Bsp1286I G_dGCh'C
Cuts at: 128 151 182 353 656 716 1089 1181 1598
Size: 23 31 171 303 60 373 92 417
Cuts at: 1598 1679 1748 2228 2546 2663 2675 2806 2840
Size: 81 69 480 318 117 12 131 34
Cuts at: 2840 3028 3639 3732 3806 3996 4058 4788 128
Size: 188 611 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 480 417 373 318 303
190 188 171 131 117 93 92 81
74 69 62 60 34 31 23 12
BspEI T'CCGG_A
Cuts at: 581 788 1763 5234 581
Size: 207 975 3471 1361
Fragments arranged by size:
3471 1361 975 207
BspHI T'CATG_A
Cuts at: 848 1520 1538 2078 5190 848
Size: 672 18 540 3112 1672
Fragments arranged by size:
3112 1672 672 540 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1489 2957 3009 3064 3278 3580 3961 1006
Size: 483 1468 52 55 214 302 381 3059
Fragments arranged by size:
3059 1468 483 381 302 214 55 52
BsrBI CCG'CTC
Cuts at: 50 338 1653 4306 4360 5844 6009 50
Size: 288 1315 2653 54 1484 165 55
Fragments arranged by size:
2653 1484 1315 288 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1364 1754 2171 3926 1364
Size: 390 417 1755 3452
Fragments arranged by size:
3452 1755 417 390
BsrFI r'CCGG_y
Cuts at: 158 871 1163 2033 2061 2111 4012 4193 4402
Size: 713 292 870 28 50 1901 181 209
Cuts at: 4402 158
Size: 1770
Fragments arranged by size:
1901 1770 870 713 292 209 181 50
28
BssSI C'ACGA_G
Cuts at: 1684 4285 4643 1684
Size: 2601 358 3055
Fragments arranged by size:
3055 2601 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1739 3391 1739
Size: 1652 4362
BstBI TT'CG_AA
Cuts at: 4375 4375
Size: 6014
BstXI CCAn_nnnn'nTGG
Cuts at: 189 1002 2220 2298 2979 189
Size: 813 1218 78 681 3224
Fragments arranged by size:
3224 1218 813 681 78
BstYI r'GATC_y
Cuts at: 1 305 410 962 1241 1958 3039 3506 3529
Size: 304 105 552 279 717 1081 467 23
Cuts at: 3529 3864 4110 5122 5208 5220 5566 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1081 1012 717 552 467 449 346 335
304 279 246 105 86 23 12
BtgI C'CryG_G
Cuts at: 182 1715 2177 2474 182
Size: 1533 462 297 3722
Fragments arranged by size:
3722 1533 462 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3964 4145 4308 3964
Size: 181 163 5670
Fragments arranged by size:
5670 181 163
BtsI GCAGTG_nn'
Cuts at: 1538 1696 3257 1538
Size: 158 1561 4295
Fragments arranged by size:
4295 1561 158
DraI TTT'AAA
Cuts at: 3013 3013
Size: 6014
DraIII CAC_nnn'GTG
Cuts at: 1566 1566
Size: 6014
DrdI GACnn_nn'nnGTC
Cuts at: 1759 3720 4578 1759
Size: 1961 858 3195
Fragments arranged by size:
3195 1961 858
EaeI y'GGCC_r
Cuts at: 1004 2981 3599 3773 4164 4191 5536 1004
Size: 1977 618 174 391 27 1345 1482
Fragments arranged by size:
1977 1482 1345 618 391 174 27
EagI C'GGCC_G
Cuts at: 3599 3599
Size: 6014
EarI CTCTTCn'nnn_
Cuts at: 1583 2348 4037 4247 1583
Size: 765 1689 210 3350
Fragments arranged by size:
3350 1689 765 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 357 955 4222 4532 4678 5824 357
Size: 598 3267 310 146 1146 547
Fragments arranged by size:
3267 1146 598 547 310 146
EcoICRI GAG'CTC
Cuts at: 654 714 2661 2673 2838 3026 654
Size: 60 1947 12 165 188 3642
Fragments arranged by size:
3642 1947 188 165 60 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 409 559 670 1051 1066 1279 1669 1690
Size: 224 150 111 381 15 213 390 21
Cuts at: 1690 1747 2014 2041 2551 2677 3839 4271 5018
Size: 57 267 27 510 126 1162 432 747
Cuts at: 5018 5286 185
Size: 268 913
Fragments arranged by size:
1162 913 747 510 432 390 381 268
267 224 213 150 126 111 57 27
21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 541 721 828 1123 2887 541
Size: 180 107 295 1764 3668
Fragments arranged by size:
3668 1764 295 180 107
EcoO109I rG'GnC_Cy
Cuts at: 169 2542 2543 3153 169
Size: 2373 1 610 3030
Fragments arranged by size:
3030 2373 610 1
EcoRI G'AATT_C
Cuts at: 3018 3018
Size: 6014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 255 723 1998 4033 255
Size: 468 1275 2035 2236
Fragments arranged by size:
2236 2035 1275 468
FspI TGC'GCA
Cuts at: 3795 3795
Size: 6014
HaeII r_GCGC'y
Cuts at: 603 3448 3696 4718 5697 603
Size: 2845 248 1022 979 920
Fragments arranged by size:
2845 1022 979 920 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 2446 2478 3856 3886 3888 3918 685
Size: 32 1729 32 1378 30 2 30 2781
Fragments arranged by size:
2781 1729 1378 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3053 3053
Size: 6014
KasI G'GCGC_C
Cuts at: 3444 3692 3444
Size: 248 5766
KpnI G_GTAC'C
Cuts at: 196 3034 196
Size: 2838 3176
MluI A'CGCG_T
Cuts at: 5447 5447
Size: 6014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 728 1929 2361 4685 4869 728
Size: 1201 432 2324 184 1873
Fragments arranged by size:
2324 1873 1201 432 184
MscI TGG'CCA
Cuts at: 2983 3775 2983
Size: 792 5222
MslI CAynn'nnrTG
Cuts at: 187 934 1684 1774 2014 2176 4003 4130 187
Size: 747 750 90 240 162 1827 127 2071
Fragments arranged by size:
2071 1827 750 747 240 162 127 90
MspA1I CmG'CkG
Cuts at: 639 905 972 1044 1071 2087 2367 2682 3125
Size: 266 67 72 27 1016 280 315 443
Cuts at: 3125 3439 3799 4812 5057 639
Size: 314 360 1013 245 1596
Fragments arranged by size:
1596 1016 1013 443 360 315 314 280
266 245 72 67 27
NaeI GCC'GGC
Cuts at: 160 873 1165 4195 160
Size: 713 292 3030 1979
Fragments arranged by size:
3030 1979 713 292
NarI GG'CG_CC
Cuts at: 3445 3693 3445
Size: 248 5766
NcoI C'CATG_G
Cuts at: 182 1715 2177 2474 182
Size: 1533 462 297 3722
Fragments arranged by size:
3722 1533 462 297
NgoMIV G'CCGG_C
Cuts at: 158 871 1163 4193 158
Size: 713 292 3030 1979
Fragments arranged by size:
3030 1979 713 292
NspI r_CATG'y
Cuts at: 597 1953 3067 3567 4474 597
Size: 1356 1114 500 907 2137
Fragments arranged by size:
2137 1356 1114 907 500
PciI A'CATG_T
Cuts at: 4470 4470
Size: 6014
PflMI CCAn_nnn'nTGG
Cuts at: 1360 1647 1877 1360
Size: 287 230 5497
Fragments arranged by size:
5497 287 230
PfoI T'CCnGG_A
Cuts at: 308 2237 2318 2402 308
Size: 1929 81 84 3920
Fragments arranged by size:
3920 1929 84 81
PmeI GTTT'AAAC
Cuts at: 3013 3013
Size: 6014
PmlI CAC'GTG
Cuts at: 474 474
Size: 6014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1648 1680 5153 5185 1648
Size: 32 3473 32 2477
Fragments arranged by size:
3473 2477 32 32
PshAI GACnn'nnGTC
Cuts at: 1811 1811
Size: 6014
PspOMI G'GGCC_C
Cuts at: 349 2542 349
Size: 2193 3821
PstI C_TGCA'G
Cuts at: 177 638 1172 1294 1703 2315 3006 3061 3746
Size: 461 534 122 409 612 691 55 685
Cuts at: 3746 177
Size: 2445
Fragments arranged by size:
2445 691 685 612 534 461 409 122
55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6014
PvuII CAG'CTG
Cuts at: 639 972 1071 2367 2682 3439 3799 639
Size: 333 99 1296 315 757 360 2854
Fragments arranged by size:
2854 1296 757 360 333 315 99
RsrII CG'GwC_CG
Cuts at: 4209 4209
Size: 6014
SacI G_AGCT'C
Cuts at: 656 716 2663 2675 2840 3028 656
Size: 60 1947 12 165 188 3642
Fragments arranged by size:
3642 1947 188 165 60 12
SalI G'TCGA_C
Cuts at: 3051 3051
Size: 6014
SapI GCTCTTCn'nnn_
Cuts at: 4037 4247 4037
Size: 210 5804
SbfI CC_TGCA'GG
Cuts at: 3061 3061
Size: 6014
ScaI AGT'ACT
Cuts at: 1064 5531 1064
Size: 4467 1547
SexAI A'CCwGG_T
Cuts at: 941 2615 941
Size: 1674 4340
SfcI C'TryA_G
Cuts at: 33 173 243 634 1168 1290 1699 2311 3002
Size: 140 70 391 534 122 409 612 691
Cuts at: 3002 3057 3742 4735 4926 33
Size: 55 685 993 191 1121
Fragments arranged by size:
1121 993 691 685 612 534 409 391
191 140 122 70 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 2989 2989
Size: 6014
SfoI GGC'GCC
Cuts at: 3446 3694 3446
Size: 248 5766
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
SmaI CCC'GGG
Cuts at: 3036 3036
Size: 6014
SmlI C'TyrA_G
Cuts at: 229 2662 3169 4084 4576 4838 229
Size: 2433 507 915 492 262 1405
Fragments arranged by size:
2433 1405 915 507 492 262
SpeI A'CTAG_T
Cuts at: 67 976 67
Size: 909 5105
SphI G_CATG'C
Cuts at: 3067 3567 3067
Size: 500 5514
StuI AGG'CCT
Cuts at: 1096 1096
Size: 6014
StyI C'CwwG_G
Cuts at: 182 1182 1204 1715 2177 2474 2972 3148 3502
Size: 1000 22 511 462 297 498 176 354
Cuts at: 3502 182
Size: 2694
Fragments arranged by size:
2694 1000 511 498 462 354 297 176
22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 430 440 2265 2821 3011 3597 3730 4189 4398
Size: 10 1825 556 190 586 133 459 209
Cuts at: 4398 430
Size: 2046
Fragments arranged by size:
2046 1825 586 556 459 209 190 133
10
TatI w'GTAC_w
Cuts at: 1062 1691 2270 5529 1062
Size: 629 579 3259 1547
Fragments arranged by size:
3259 1547 629 579
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 385 417 469 501 826 858 1200 1232 2425
Size: 32 52 32 325 32 342 32 1193
Cuts at: 2425 2457 2564 2596 5531 5563 385
Size: 32 107 32 2935 32 836
Fragments arranged by size:
2935 1193 836 342 325 107 52 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 943 3811 943
Size: 2868 3146
XbaI T'CTAG_A
Cuts at: 3045 3045
Size: 6014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 1254 1722 2595 3509 360
Size: 894 468 873 914 2865
Fragments arranged by size:
2865 914 894 873 468
XmaI C'CCGG_G
Cuts at: 3034 3034
Size: 6014
XmnI GAAnn'nnTTC
Cuts at: 271 856 914 271
Size: 585 58 5371
Fragments arranged by size:
5371 585 58
ZraI GAC'GTC
Cuts at: 1929 1929
Size: 6014
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI BcgI BfrBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PsiI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI SwaI XhoI