Restriction map for
pF1KB8560
(Circular) (Six-base) MAPSORT of: pF1KB8560.seq Check: 2905 from: 1 to: 5843
pF1KB8560 5843 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 713 713
Size: 5843
AccI GT'mk_AC
Cuts at: 2486 2881 2486
Size: 395 5448
Acc65I G'GTAC_C
Cuts at: 2859 2859
Size: 5843
AclI AA'CG_TT
Cuts at: 5683 5683
Size: 5843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 2740 3668 4100 4847 5115 696
Size: 2044 928 432 747 268 1424
Fragments arranged by size:
2044 1424 928 747 432 268
AflIII A'CryG_T
Cuts at: 3195 4299 5276 3195
Size: 1104 977 3762
Fragments arranged by size:
3762 1104 977
AgeI A'CCGG_T
Cuts at: 4231 4231
Size: 5843
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 5811
AlwNI CAG_nnn'CTG
Cuts at: 1817 2344 3148 4715 1817
Size: 527 804 1567 2945
Fragments arranged by size:
2945 1567 804 527
ApaLI G'TGCA_C
Cuts at: 4613 4613
Size: 5843
ApoI r'AATT_y
Cuts at: 144 163 579 619 888 1225 1294 1378 1513
Size: 19 416 40 269 337 69 84 135
Cuts at: 1513 1797 2538 2638 2847 5196 5452 144
Size: 284 741 100 209 2349 256 535
Fragments arranged by size:
2349 741 535 416 337 284 269 256
209 135 100 84 69 40 19
AseI AT'TA_AT
Cuts at: 20 254 1525 20
Size: 234 1271 4338
Fragments arranged by size:
4338 1271 234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
AvaI C'yCGr_G
Cuts at: 2863 2863
Size: 5843
BamHI G'GATC_C
Cuts at: 2868 2868
Size: 5843
BanI G'GyrC_C
Cuts at: 2859 3273 3521 3556 2859
Size: 414 248 35 5146
Fragments arranged by size:
5146 414 248 35
BanII G_rGCy'C
Cuts at: 1869 2857 3887 1869
Size: 988 1030 3825
Fragments arranged by size:
3825 1030 988
BbeI G_GCGC'C
Cuts at: 3277 3525 3277
Size: 248 5595
BbsI GAAGACnn'nnnn_
Cuts at: 1108 1183 1108
Size: 75 5768
BciVI GTATCCnnnnn_n'
Cuts at: 2115 3741 4502 5843 2115
Size: 1626 761 1341 2115
Fragments arranged by size:
2115 1626 1341 761
BclI T'GATC_A
Cuts at: 1272 3363 1272
Size: 2091 3752
BglII A'GATC_T
Cuts at: 2438 3358 5395 2438
Size: 920 2037 2886
Fragments arranged by size:
2886 2037 920
BlpI GC'TnA_GC
Cuts at: 2955 2955
Size: 5843
Bme1580I G_kGCm'C
Cuts at: 3468 3561 4617 3468
Size: 93 1056 4694
Fragments arranged by size:
4694 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3055 3136 3471 5615 3055
Size: 81 335 2144 3283
Fragments arranged by size:
3283 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4210 4210
Size: 5843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 686 2762 158
Size: 528 2076 3239
Fragments arranged by size:
3239 2076 528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 3019 3898 4390 4688 2807
Size: 212 879 492 298 3962
Fragments arranged by size:
3962 879 492 298 212
BsaI GGTCTCn'nnnn_
Cuts at: 5547 5547
Size: 5843
BsaAI yAC'GTr
Cuts at: 3196 3196
Size: 5843
BsaBI GATnn'nnATC
Cuts at: 6 917 2333 3357 5394 6
Size: 911 1416 1024 2037 455
Fragments arranged by size:
2037 1416 1024 911 455
BsaHI Gr'CG_yC
Cuts at: 3274 3522 4224 5720 3274
Size: 248 702 1496 3397
Fragments arranged by size:
3397 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3032 3553 4231 4505 4652 5063 3032
Size: 521 678 274 147 411 3812
Fragments arranged by size:
3812 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1473 1503 2364 2394 2597 2627 1473
Size: 30 861 30 203 30 4689
Fragments arranged by size:
4689 861 203 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 755 755
Size: 5843
BseYI C'CCAG_C
Cuts at: 2714 3268 4026 4603 5099 2714
Size: 554 758 577 496 3458
Fragments arranged by size:
3458 758 577 554 496
BsiEI CG_ry'CG
Cuts at: 104 3385 3431 4639 104
Size: 3281 46 1208 1308
Fragments arranged by size:
3281 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1767 1869 2857 3635 3825 4617 1767
Size: 102 988 778 190 792 2993
Fragments arranged by size:
2993 988 792 778 190 102
BsmI GAATG_Cn'
Cuts at: 762 762
Size: 5843
BsmBI CGTCTCn'nnnn_
Cuts at: 105 105
Size: 5843
Bsp1286I G_dGCh'C
Cuts at: 1767 1869 2857 3468 3561 3635 3825 3887 4617
Size: 102 988 611 93 74 190 62 730
Cuts at: 4617 1767
Size: 2993
Fragments arranged by size:
2993 988 730 611 190 102 93 74
62
BspEI T'CCGG_A
Cuts at: 5063 5063
Size: 5843
BspHI T'CATG_A
Cuts at: 2577 5019 2577
Size: 2442 3401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 713 2893 3107 3409 3790 713
Size: 2180 214 302 381 2766
Fragments arranged by size:
2766 2180 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4135 4189 5673 5838 50
Size: 4085 54 1484 165 55
Fragments arranged by size:
4085 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1105 1117 2363 2731 3755 1105
Size: 12 1246 368 1024 3193
Fragments arranged by size:
3193 1246 1024 368 12
BsrFI r'CCGG_y
Cuts at: 3841 4022 4231 3841
Size: 181 209 5453
Fragments arranged by size:
5453 209 181
BsrGI T'GTAC_A
Cuts at: 2394 2599 2394
Size: 205 5638
BssSI C'ACGA_G
Cuts at: 4114 4472 4114
Size: 358 5485
BstAPI GCAn_nnn'nTGC
Cuts at: 3220 3220
Size: 5843
BstBI TT'CG_AA
Cuts at: 4204 4204
Size: 5843
BstYI r'GATC_y
Cuts at: 1 396 2302 2438 2763 2868 3335 3358 3693
Size: 395 1906 136 325 105 467 23 335
Cuts at: 3693 3939 4951 5037 5049 5395 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1906 1012 467 449 395 346 335 325
246 136 105 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3793 3974 4137 3793
Size: 181 163 5499
Fragments arranged by size:
5499 181 163
BtsI GCAGTG_nn'
Cuts at: 360 443 723 1144 3086 360
Size: 83 280 421 1942 3117
Fragments arranged by size:
3117 1942 421 280 83
DraI TTT'AAA
Cuts at: 302 2753 2842 302
Size: 2451 89 3303
Fragments arranged by size:
3303 2451 89
DrdI GACnn_nn'nnGTC
Cuts at: 3549 4407 3549
Size: 858 4985
EaeI y'GGCC_r
Cuts at: 3428 3602 3993 4020 5365 3428
Size: 174 391 27 1345 3906
Fragments arranged by size:
3906 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3428 3428
Size: 5843
EarI CTCTTCn'nnn_
Cuts at: 197 449 597 638 1644 2244 3866 4076 197
Size: 252 148 41 1006 600 1622 210 1964
Fragments arranged by size:
1964 1622 1006 600 252 210 148 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4051 4361 4507 5653 4051
Size: 310 146 1146 4241
Fragments arranged by size:
4241 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1867 2855 1867
Size: 988 4855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 686 696 2740 2762 3668 4100 4847 5115
Size: 528 10 2044 22 906 432 747 268
Cuts at: 5115 158
Size: 886
Fragments arranged by size:
2044 906 886 747 528 432 268 22
10
EcoO109I rG'GnC_Cy
Cuts at: 1147 2982 1147
Size: 1835 4008
EcoRI G'AATT_C
Cuts at: 144 1294 2847 144
Size: 1150 1553 3140
Fragments arranged by size:
3140 1553 1150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3862 3862
Size: 5843
FspI TGC'GCA
Cuts at: 3624 3624
Size: 5843
HaeII r_GCGC'y
Cuts at: 3277 3525 4547 5526 3277
Size: 248 1022 979 3594
Fragments arranged by size:
3594 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1670 1702 2276 2308 2364 2396 3685 3715 3717
Size: 32 574 32 56 32 1289 30 2
Cuts at: 3717 3747 1670
Size: 30 3766
Fragments arranged by size:
3766 1289 574 56 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2233 2487 2882 2233
Size: 254 395 5194
Fragments arranged by size:
5194 395 254
HindIII A'AGCT_T
Cuts at: 1200 1200
Size: 5843
KasI G'GCGC_C
Cuts at: 3273 3521 3273
Size: 248 5595
KpnI G_GTAC'C
Cuts at: 2863 2863
Size: 5843
MluI A'CGCG_T
Cuts at: 5276 5276
Size: 5843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2132 2612 4514 4698 1814
Size: 318 480 1902 184 2959
Fragments arranged by size:
2959 1902 480 318 184
MscI TGG'CCA
Cuts at: 3604 3604
Size: 5843
MslI CAynn'nnrTG
Cuts at: 1110 2159 2621 3832 3959 1110
Size: 1049 462 1211 127 2994
Fragments arranged by size:
2994 1211 1049 462 127
MspA1I CmG'CkG
Cuts at: 1657 2718 2954 3268 3628 4641 4886 1657
Size: 1061 236 314 360 1013 245 2614
Fragments arranged by size:
2614 1061 1013 360 314 245 236
NaeI GCC'GGC
Cuts at: 4024 4024
Size: 5843
NarI GG'CG_CC
Cuts at: 3274 3522 3274
Size: 248 5595
NgoMIV G'CCGG_C
Cuts at: 4022 4022
Size: 5843
NspI r_CATG'y
Cuts at: 2896 3396 4303 2896
Size: 500 907 4436
Fragments arranged by size:
4436 907 500
PciI A'CATG_T
Cuts at: 4299 4299
Size: 5843
PfoI T'CCnGG_A
Cuts at: 174 1542 174
Size: 1368 4475
PmeI GTTT'AAAC
Cuts at: 2842 2842
Size: 5843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 4982 5014 2597
Size: 32 2353 32 3426
Fragments arranged by size:
3426 2353 32 32
PpuMI rG'GwC_Cy
Cuts at: 1147 1147
Size: 5843
PsiI TTA'TAA
Cuts at: 617 1711 617
Size: 1094 4749
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 807 839 807
Size: 32 5811
PstI C_TGCA'G
Cuts at: 123 1139 2890 3575 123
Size: 1016 1751 685 2391
Fragments arranged by size:
2391 1751 1016 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5843
PvuII CAG'CTG
Cuts at: 1657 2718 3268 3628 1657
Size: 1061 550 360 3872
Fragments arranged by size:
3872 1061 550 360
RsrII CG'GwC_CG
Cuts at: 4038 4038
Size: 5843
SacI G_AGCT'C
Cuts at: 1869 2857 1869
Size: 988 4855
SalI G'TCGA_C
Cuts at: 2485 2880 2485
Size: 395 5448
SanDI GG'GwC_CC
Cuts at: 1147 1147
Size: 5843
SapI GCTCTTCn'nnn_
Cuts at: 1644 3866 4076 1644
Size: 2222 210 3411
Fragments arranged by size:
3411 2222 210
SbfI CC_TGCA'GG
Cuts at: 2890 2890
Size: 5843
ScaI AGT'ACT
Cuts at: 324 750 5360 324
Size: 426 4610 807
Fragments arranged by size:
4610 807 426
SfcI C'TryA_G
Cuts at: 33 119 1135 1439 1768 2038 2417 2886 3571
Size: 86 1016 304 329 270 379 469 685
Cuts at: 3571 4564 4755 33
Size: 993 191 1121
Fragments arranged by size:
1121 1016 993 685 469 379 329 304
270 191 86
SfoI GGC'GCC
Cuts at: 3275 3523 3275
Size: 248 5595
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
SmaI CCC'GGG
Cuts at: 2865 2865
Size: 5843
SmlI C'TyrA_G
Cuts at: 2822 2998 3913 4405 4667 2822
Size: 176 915 492 262 3998
Fragments arranged by size:
3998 915 492 262 176
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5843
SphI G_CATG'C
Cuts at: 2896 3396 2896
Size: 500 5343
SspI AAT'ATT
Cuts at: 364 481 1550 364
Size: 117 1069 4657
Fragments arranged by size:
4657 1069 117
StyI C'CwwG_G
Cuts at: 443 2977 3331 443
Size: 2534 354 2955
Fragments arranged by size:
2955 2534 354
SwaI ATTT'AAAT
Cuts at: 2753 2753
Size: 5843
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 679 3426 3559 4018 4227 679
Size: 2747 133 459 209 2295
Fragments arranged by size:
2747 2295 459 209 133
TatI w'GTAC_w
Cuts at: 322 748 2394 2599 5358 322
Size: 426 1646 205 2759 807
Fragments arranged by size:
2759 1646 807 426 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1190 1222 5360 5392 1190
Size: 32 4138 32 1641
Fragments arranged by size:
4138 1641 32 32
Tth111I GACn'n_nGTC
Cuts at: 3640 3640
Size: 5843
XbaI T'CTAG_A
Cuts at: 2362 2650 2874 2362
Size: 288 224 5331
Fragments arranged by size:
5331 288 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3338 3338
Size: 5843
XmaI C'CCGG_G
Cuts at: 2863 2863
Size: 5843
XmnI GAAnn'nnTTC
Cuts at: 148 787 847 946 2510 2612 148
Size: 639 60 99 1564 102 3379
Fragments arranged by size:
3379 1564 639 102 99 60
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PspOMI PspXI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI XhoI ZraI