Restriction map for
pF1KB8556
(Circular) (Six-base) MAPSORT of: pF1KB8556.seq Check: 2205 from: 1 to: 5843
pF1KB8556 5843 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 1505 1505
Size: 5843
AccI GT'mk_AC
Cuts at: 2881 2881
Size: 5843
Acc65I G'GTAC_C
Cuts at: 2859 2859
Size: 5843
AclI AA'CG_TT
Cuts at: 5683 5683
Size: 5843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1726 2322 2342 3668 4100 4847 5115 979
Size: 747 596 20 1326 432 747 268 1707
Fragments arranged by size:
1707 1326 747 747 596 432 268 20
AflIII A'CryG_T
Cuts at: 3195 4299 5276 3195
Size: 1104 977 3762
Fragments arranged by size:
3762 1104 977
AgeI A'CCGG_T
Cuts at: 4231 4231
Size: 5843
AhdI GACnn_n'nnGTC
Cuts at: 1581 1581
Size: 5843
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 2274
Size: 32 5811
AlwNI CAG_nnn'CTG
Cuts at: 608 2715 3148 4715 608
Size: 2107 433 1567 1736
Fragments arranged by size:
2107 1736 1567 433
ApaI G_GGCC'C
Cuts at: 1062 1062
Size: 5843
ApaLI G'TGCA_C
Cuts at: 353 4613 353
Size: 4260 1583
ApoI r'AATT_y
Cuts at: 153 390 412 496 2847 5196 5452 153
Size: 237 22 84 2351 2349 256 544
Fragments arranged by size:
2351 2349 544 256 237 84 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 5843
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
AvaI C'yCGr_G
Cuts at: 1023 1420 2530 2557 2670 2863 1023
Size: 397 1110 27 113 193 4003
Fragments arranged by size:
4003 1110 397 193 113 27
BamHI G'GATC_C
Cuts at: 1959 2175 2868 1959
Size: 216 693 4934
Fragments arranged by size:
4934 693 216
BanI G'GyrC_C
Cuts at: 114 769 918 1825 2859 3273 3521 3556 114
Size: 655 149 907 1034 414 248 35 2401
Fragments arranged by size:
2401 1034 907 655 414 248 149 35
BanII G_rGCy'C
Cuts at: 690 704 1062 1421 1821 2857 3887 690
Size: 14 358 359 400 1036 1030 2646
Fragments arranged by size:
2646 1036 1030 400 359 358 14
BbeI G_GCGC'C
Cuts at: 118 773 3277 3525 118
Size: 655 2504 248 2436
Fragments arranged by size:
2504 2436 655 248
BbsI GAAGACnn'nnnn_
Cuts at: 760 967 1598 1982 760
Size: 207 631 384 4621
Fragments arranged by size:
4621 631 384 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 628 662 628
Size: 34 5809
BciVI GTATCCnnnnn_n'
Cuts at: 3741 4502 5843 3741
Size: 761 1341 3741
Fragments arranged by size:
3741 1341 761
BclI T'GATC_A
Cuts at: 3363 3363
Size: 5843
BglI GCCn_nnn'nGGC
Cuts at: 112 469 112
Size: 357 5486
BglII A'GATC_T
Cuts at: 1010 3358 5395 1010
Size: 2348 2037 1458
Fragments arranged by size:
2348 2037 1458
BlpI GC'TnA_GC
Cuts at: 1495 2812 2955 1495
Size: 1317 143 4383
Fragments arranged by size:
4383 1317 143
Bme1580I G_kGCm'C
Cuts at: 357 641 710 923 1062 3468 3561 4617 357
Size: 284 69 213 139 2406 93 1056 1583
Fragments arranged by size:
2406 1583 1056 284 213 139 93 69
BmrI ACTGGGnnnn_n'
Cuts at: 477 921 1284 3055 3136 3471 5615 477
Size: 444 363 1771 81 335 2144 705
Fragments arranged by size:
2144 1771 705 444 363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4210 4210
Size: 5843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 712 1672 1810 2232 566
Size: 146 960 138 422 4177
Fragments arranged by size:
4177 960 422 146 138
Bpu10I CC'TnA_GC
Cuts at: 1852 1852
Size: 5843
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 871 1145 1427 3019 3898 4390 4688 399
Size: 472 274 282 1592 879 492 298 1554
Fragments arranged by size:
1592 1554 879 492 472 298 282 274
BsaI GGTCTCn'nnnn_
Cuts at: 5547 5547
Size: 5843
BsaAI yAC'GTr
Cuts at: 987 3196 987
Size: 2209 3634
BsaBI GATnn'nnATC
Cuts at: 6 3357 5394 6
Size: 3351 2037 455
Fragments arranged by size:
3351 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 770 1502 1862 3274 3522 4224 5720 115
Size: 655 732 360 1412 248 702 1496 238
Fragments arranged by size:
1496 1412 732 702 655 360 248 238
BsaWI w'CCGG_w
Cuts at: 1956 3032 3553 4231 4505 4652 5063 1956
Size: 1076 521 678 274 147 411 2736
Fragments arranged by size:
2736 1076 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 539 569 539
Size: 30 5813
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1148 2247 2370 2453 2628 2719 1148
Size: 1099 123 83 175 91 4272
Fragments arranged by size:
4272 1099 175 123 91 83
BseYI C'CCAG_C
Cuts at: 134 1646 1669 1751 2482 3268 4026 4603 5099
Size: 1512 23 82 731 786 758 577 496
Cuts at: 5099 134
Size: 878
Fragments arranged by size:
1512 878 786 758 731 577 496 82
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 1390
Size: 5843
BsiEI CG_ry'CG
Cuts at: 104 3385 3431 4639 104
Size: 3281 46 1208 1308
Fragments arranged by size:
3281 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 357 1421 1811 2857 3635 3825 4617 357
Size: 1064 390 1046 778 190 792 1583
Fragments arranged by size:
1583 1064 1046 792 778 390 190
BsmI GAATG_Cn'
Cuts at: 1661 1661
Size: 5843
BsmBI CGTCTCn'nnnn_
Cuts at: 1869 1869
Size: 5843
Bsp1286I G_dGCh'C
Cuts at: 357 641 690 704 710 923 1062 1421 1811
Size: 284 49 14 6 213 139 359 390
Cuts at: 1811 1821 2857 3468 3561 3635 3825 3887 4617
Size: 10 1036 611 93 74 190 62 730
Cuts at: 4617 357
Size: 1583
Fragments arranged by size:
1583 1036 730 611 390 359 284 213
190 139 93 74 62 49 14 10
6
BspEI T'CCGG_A
Cuts at: 1956 5063 1956
Size: 3107 2736
BspHI T'CATG_A
Cuts at: 569 5019 569
Size: 4450 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 171 2466 2893 3107 3409 3790 171
Size: 2295 427 214 302 381 2224
Fragments arranged by size:
2295 2224 427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 120 818 4135 4189 5673 5838 50
Size: 70 698 3317 54 1484 165 55
Fragments arranged by size:
3317 1484 698 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 2706 3755 2706
Size: 1049 4794
BsrFI r'CCGG_y
Cuts at: 3841 4022 4231 3841
Size: 181 209 5453
Fragments arranged by size:
5453 209 181
BssSI C'ACGA_G
Cuts at: 4114 4472 4114
Size: 358 5485
BstAPI GCAn_nnn'nTGC
Cuts at: 1950 3220 1950
Size: 1270 4573
BstBI TT'CG_AA
Cuts at: 2206 4204 2206
Size: 1998 3845
BstXI CCAn_nnnn'nTGG
Cuts at: 675 675
Size: 5843
BstYI r'GATC_y
Cuts at: 1 1010 1959 2073 2175 2271 2289 2868 3335
Size: 1009 949 114 102 96 18 579 467
Cuts at: 3335 3358 3693 3939 4951 5037 5049 5395 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 1009 949 579 467 449 346 335
246 114 102 96 86 23 18 12
BtgI C'CryG_G
Cuts at: 107 831 923 1821 1895 2115 2467 107
Size: 724 92 898 74 220 352 3483
Fragments arranged by size:
3483 898 724 352 220 92 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2790 3793 3974 4137 2790
Size: 1003 181 163 4496
Fragments arranged by size:
4496 1003 181 163
BtsI GCAGTG_nn'
Cuts at: 3086 3086
Size: 5843
DraI TTT'AAA
Cuts at: 2001 2418 2842 2001
Size: 417 424 5002
Fragments arranged by size:
5002 424 417
DraIII CAC_nnn'GTG
Cuts at: 361 678 361
Size: 317 5526
DrdI GACnn_nn'nnGTC
Cuts at: 2629 3549 4407 2629
Size: 920 858 4065
Fragments arranged by size:
4065 920 858
EaeI y'GGCC_r
Cuts at: 284 363 665 993 1064 1844 2801 3428 3602
Size: 79 302 328 71 780 957 627 174
Cuts at: 3602 3993 4020 5365 284
Size: 391 27 1345 762
Fragments arranged by size:
1345 957 780 762 627 391 328 302
174 79 71 27
EagI C'GGCC_G
Cuts at: 3428 3428
Size: 5843
EarI CTCTTCn'nnn_
Cuts at: 1334 3866 4076 1334
Size: 2532 210 3101
Fragments arranged by size:
3101 2532 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 702 2761 4051 4361 4507 5653 702
Size: 2059 1290 310 146 1146 892
Fragments arranged by size:
2059 1290 1146 892 310 146
EcoICRI GAG'CTC
Cuts at: 1419 2855 1419
Size: 1436 4407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 712 979 1672 1726 1810 2232 2322 2342
Size: 146 267 693 54 84 422 90 20
Cuts at: 2342 3668 4100 4847 5115 566
Size: 1326 432 747 268 1294
Fragments arranged by size:
1326 1294 747 693 432 422 268 267
146 90 84 54 20
EcoNI CCTnn'n_nnAGG
Cuts at: 694 694
Size: 5843
EcoO109I rG'GnC_Cy
Cuts at: 612 1059 2088 2306 2535 2604 2790 2982 612
Size: 447 1029 218 229 69 186 192 3473
Fragments arranged by size:
3473 1029 447 229 218 192 186 69
EcoRI G'AATT_C
Cuts at: 2847 2847
Size: 5843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 244 1029 3862 244
Size: 785 2833 2225
Fragments arranged by size:
2833 2225 785
FspI TGC'GCA
Cuts at: 3624 3624
Size: 5843
HaeII r_GCGC'y
Cuts at: 118 773 3277 3525 4547 5526 118
Size: 655 2504 248 1022 979 435
Fragments arranged by size:
2504 1022 979 655 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 539 571 1567 1599 2674 2706 3685 3715 3717
Size: 32 996 32 1075 32 979 30 2
Cuts at: 3717 3747 539
Size: 30 2635
Fragments arranged by size:
2635 1075 996 979 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1712 2882 1712
Size: 1170 4673
KasI G'GCGC_C
Cuts at: 114 769 3273 3521 114
Size: 655 2504 248 2436
Fragments arranged by size:
2504 2436 655 248
KpnI G_GTAC'C
Cuts at: 2863 2863
Size: 5843
MluI A'CGCG_T
Cuts at: 5276 5276
Size: 5843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1595 4514 4698 1595
Size: 2919 184 2740
Fragments arranged by size:
2919 2740 184
MscI TGG'CCA
Cuts at: 365 667 995 1846 2803 3604 365
Size: 302 328 851 957 801 2604
Fragments arranged by size:
2604 957 851 801 328 302
MslI CAynn'nnrTG
Cuts at: 1053 3832 3959 1053
Size: 2779 127 2937
Fragments arranged by size:
2937 2779 127
MspA1I CmG'CkG
Cuts at: 1650 1683 1755 2486 2570 2715 2954 3268 3628
Size: 33 72 731 84 145 239 314 360
Cuts at: 3628 4641 4886 1650
Size: 1013 245 2607
Fragments arranged by size:
2607 1013 731 360 314 245 239 145
84 72 33
NaeI GCC'GGC
Cuts at: 4024 4024
Size: 5843
NarI GG'CG_CC
Cuts at: 115 770 3274 3522 115
Size: 655 2504 248 2436
Fragments arranged by size:
2504 2436 655 248
NcoI C'CATG_G
Cuts at: 107 1821 1895 107
Size: 1714 74 4055
Fragments arranged by size:
4055 1714 74
NdeI CA'TA_TG
Cuts at: 1054 1054
Size: 5843
NgoMIV G'CCGG_C
Cuts at: 4022 4022
Size: 5843
NspI r_CATG'y
Cuts at: 402 1494 2829 2896 3396 4303 402
Size: 1092 1335 67 500 907 1942
Fragments arranged by size:
1942 1335 1092 907 500 67
PciI A'CATG_T
Cuts at: 4299 4299
Size: 5843
PflMI CCAn_nnn'nTGG
Cuts at: 2116 2365 2116
Size: 249 5594
PmeI GTTT'AAAC
Cuts at: 2842 2842
Size: 5843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 4982
Size: 32 5811
PpuMI rG'GwC_Cy
Cuts at: 612 2088 2306 2604 2790 612
Size: 1476 218 298 186 3665
Fragments arranged by size:
3665 1476 298 218 186
PshAI GACnn'nnGTC
Cuts at: 1274 1274
Size: 5843
PspOMI G'GGCC_C
Cuts at: 1058 1058
Size: 5843
PspXI vC'TCGA_Gb
Cuts at: 1420 1420
Size: 5843
PstI C_TGCA'G
Cuts at: 459 2646 2890 3575 459
Size: 2187 244 685 2727
Fragments arranged by size:
2727 2187 685 244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5843
PvuII CAG'CTG
Cuts at: 1650 1683 1755 2715 3268 3628 1650
Size: 33 72 960 553 360 3865
Fragments arranged by size:
3865 960 553 360 72 33
RsrII CG'GwC_CG
Cuts at: 4038 4038
Size: 5843
SacI G_AGCT'C
Cuts at: 1421 2857 1421
Size: 1436 4407
SalI G'TCGA_C
Cuts at: 2880 2880
Size: 5843
SanDI GG'GwC_CC
Cuts at: 2088 2604 2088
Size: 516 5327
SapI GCTCTTCn'nnn_
Cuts at: 3866 4076 3866
Size: 210 5633
SbfI CC_TGCA'GG
Cuts at: 2890 2890
Size: 5843
ScaI AGT'ACT
Cuts at: 5360 5360
Size: 5843
SexAI A'CCwGG_T
Cuts at: 1802 1802
Size: 5843
SfcI C'TryA_G
Cuts at: 33 455 2642 2886 3571 4564 4755 33
Size: 422 2187 244 685 993 191 1121
Fragments arranged by size:
2187 1121 993 685 422 244 191
SfoI GGC'GCC
Cuts at: 116 771 3275 3523 116
Size: 655 2504 248 2436
Fragments arranged by size:
2504 2436 655 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
SmaI CCC'GGG
Cuts at: 2865 2865
Size: 5843
SmlI C'TyrA_G
Cuts at: 378 886 1160 1420 1442 2998 3913 4405 4667
Size: 508 274 260 22 1556 915 492 262
Cuts at: 4667 378
Size: 1554
Fragments arranged by size:
1556 1554 915 508 492 274 262 260
22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5843
SphI G_CATG'C
Cuts at: 402 2896 3396 402
Size: 2494 500 2849
Fragments arranged by size:
2849 2494 500
SspI AAT'ATT
Cuts at: 1309 1309
Size: 5843
StuI AGG'CCT
Cuts at: 2662 2662
Size: 5843
StyI C'CwwG_G
Cuts at: 107 264 1821 1895 2384 2977 3331 107
Size: 157 1557 74 489 593 354 2619
Fragments arranged by size:
2619 1557 593 489 354 157 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 979 1048 1852 3426 3559 4018 4227 903
Size: 76 69 804 1574 133 459 209 2519
Fragments arranged by size:
2519 1574 804 459 209 133 76 69
TatI w'GTAC_w
Cuts at: 2494 5358 2494
Size: 2864 2979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5360 5392 5360
Size: 32 5811
Tth111I GACn'n_nGTC
Cuts at: 553 1180 3640 553
Size: 627 2460 2756
Fragments arranged by size:
2756 2460 627
XbaI T'CTAG_A
Cuts at: 2874 2874
Size: 5843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 908 1810 3338 908
Size: 902 1528 3413
Fragments arranged by size:
3413 1528 902
XhoI C'TCGA_G
Cuts at: 1420 1420
Size: 5843
XmaI C'CCGG_G
Cuts at: 2863 2863
Size: 5843
ZraI GAC'GTC
Cuts at: 1503 1503
Size: 5843
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PsiI PsrI SacII SfiI SgrAI SnaBI
SrfI SwaI XmnI