Restriction map for
pF1KB8550
(Circular) (Six-base) MAPSORT of: pF1KB8550.seq Check: 6732 from: 1 to: 5831
pF1KB8550 5831 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 609 1125 609
Size: 516 5315
AccI GT'mk_AC
Cuts at: 545 2869 545
Size: 2324 3507
Acc65I G'GTAC_C
Cuts at: 2847 2847
Size: 5831
AclI AA'CG_TT
Cuts at: 5671 5671
Size: 5831
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1770 2109 3656 4088 4835 5103 460
Size: 1310 339 1547 432 747 268 1188
Fragments arranged by size:
1547 1310 1188 747 432 339 268
AfeI AGC'GCT
Cuts at: 2505 2505
Size: 5831
AflIII A'CryG_T
Cuts at: 3183 4287 5264 3183
Size: 1104 977 3750
Fragments arranged by size:
3750 1104 977
AgeI A'CCGG_T
Cuts at: 136 4219 136
Size: 4083 1748
AleI CACnn'nnGTG
Cuts at: 946 946
Size: 5831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 137 137
Size: 5831
AlwNI CAG_nnn'CTG
Cuts at: 604 681 1026 3136 4703 604
Size: 77 345 2110 1567 1732
Fragments arranged by size:
2110 1732 1567 345 77
ApaI G_GGCC'C
Cuts at: 417 417
Size: 5831
ApaLI G'TGCA_C
Cuts at: 4601 4601
Size: 5831
ApoI r'AATT_y
Cuts at: 538 832 2835 5184 5440 538
Size: 294 2003 2349 256 929
Fragments arranged by size:
2349 2003 929 294 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5831
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
AvaI C'yCGr_G
Cuts at: 932 1505 2851 932
Size: 573 1346 3912
Fragments arranged by size:
3912 1346 573
AvrII C'CTAG_G
Cuts at: 144 144
Size: 5831
BamHI G'GATC_C
Cuts at: 2856 2856
Size: 5831
BanI G'GyrC_C
Cuts at: 160 847 2847 3261 3509 3544 160
Size: 687 2000 414 248 35 2447
Fragments arranged by size:
2447 2000 687 414 248 35
BanII G_rGCy'C
Cuts at: 263 417 1128 1506 2845 3875 263
Size: 154 711 378 1339 1030 2219
Fragments arranged by size:
2219 1339 1030 711 378 154
BbeI G_GCGC'C
Cuts at: 851 3265 3513 851
Size: 2414 248 3169
Fragments arranged by size:
3169 2414 248
BbsI GAAGACnn'nnnn_
Cuts at: 1683 1683
Size: 5831
BbvCI CC'TCA_GC
Cuts at: 1010 2515 1010
Size: 1505 4326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5797
BciVI GTATCCnnnnn_n'
Cuts at: 588 652 3729 4490 5831 588
Size: 64 3077 761 1341 588
Fragments arranged by size:
3077 1341 761 588 64
BclI T'GATC_A
Cuts at: 3351 3351
Size: 5831
BglI GCCn_nnn'nGGC
Cuts at: 2008 2008
Size: 5831
BglII A'GATC_T
Cuts at: 3346 5383 3346
Size: 2037 3794
BlpI GC'TnA_GC
Cuts at: 2943 2943
Size: 5831
Bme1580I G_kGCm'C
Cuts at: 417 3456 3549 4605 417
Size: 3039 93 1056 1643
Fragments arranged by size:
3039 1643 1056 93
BmgBI CAC'GTC
Cuts at: 423 423
Size: 5831
BmrI ACTGGGnnnn_n'
Cuts at: 330 3043 3124 3459 5603 330
Size: 2713 81 335 2144 558
Fragments arranged by size:
2713 2144 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4198 4198
Size: 5831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1495 2281 703
Size: 792 786 4253
Fragments arranged by size:
4253 792 786
Bpu10I CC'TnA_GC
Cuts at: 1010 2515 1010
Size: 1505 4326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 3007 3886 4378 4676 1357
Size: 1650 879 492 298 2512
Fragments arranged by size:
2512 1650 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 929 954 1502 5535 929
Size: 25 548 4033 1225
Fragments arranged by size:
4033 1225 548 25
BsaAI yAC'GTr
Cuts at: 1209 1455 3184 1209
Size: 246 1729 3856
Fragments arranged by size:
3856 1729 246
BsaBI GATnn'nnATC
Cuts at: 6 1114 1651 3345 5382 6
Size: 1108 537 1694 2037 455
Fragments arranged by size:
2037 1694 1108 537 455
BsaHI Gr'CG_yC
Cuts at: 848 1185 1958 3262 3510 4212 5708 848
Size: 337 773 1304 248 702 1496 971
Fragments arranged by size:
1496 1304 971 773 702 337 248
BsaWI w'CCGG_w
Cuts at: 136 662 3020 3541 4219 4493 4640 5051 136
Size: 526 2358 521 678 274 147 411 916
Fragments arranged by size:
2358 916 678 526 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1535 1565 1745 1775 1535
Size: 30 180 30 5591
Fragments arranged by size:
5591 180 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 1980 2224 667
Size: 1313 244 4274
Fragments arranged by size:
4274 1313 244
BseYI C'CCAG_C
Cuts at: 1066 1086 1297 3256 4014 4591 5087 1066
Size: 20 211 1959 758 577 496 1810
Fragments arranged by size:
1959 1810 758 577 496 211 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 1186 2075 117
Size: 1069 889 3873
Fragments arranged by size:
3873 1069 889
BsiEI CG_ry'CG
Cuts at: 104 564 3373 3419 4627 104
Size: 460 2809 46 1208 1308
Fragments arranged by size:
2809 1308 1208 460 46
BsiHKAI G_wGCw'C
Cuts at: 1128 2845 3623 3813 4605 1128
Size: 1717 778 190 792 2354
Fragments arranged by size:
2354 1717 792 778 190
BsmI GAATG_Cn'
Cuts at: 1068 1068
Size: 5831
Bsp1286I G_dGCh'C
Cuts at: 263 417 1128 1506 2845 3456 3549 3623 3813
Size: 154 711 378 1339 611 93 74 190
Cuts at: 3813 3875 4605 263
Size: 62 730 1489
Fragments arranged by size:
1489 1339 730 711 611 378 190 154
93 74 62
BspEI T'CCGG_A
Cuts at: 5051 5051
Size: 5831
BspHI T'CATG_A
Cuts at: 1655 5007 1655
Size: 3352 2479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 609 1125 2881 3095 3397 3778 609
Size: 516 1756 214 302 381 2662
Fragments arranged by size:
2662 1756 516 381 302 214
BsrBI CCG'CTC
Cuts at: 50 699 4123 4177 5661 5826 50
Size: 649 3424 54 1484 165 55
Fragments arranged by size:
3424 1484 649 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1407 2348 2526 3743 1407
Size: 941 178 1217 3495
Fragments arranged by size:
3495 1217 941 178
BsrFI r'CCGG_y
Cuts at: 136 3829 4010 4219 136
Size: 3693 181 209 1748
Fragments arranged by size:
3693 1748 209 181
BssSI C'ACGA_G
Cuts at: 4102 4460 4102
Size: 358 5473
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 1444 3208 1137
Size: 307 1764 3760
Fragments arranged by size:
3760 1764 307
BstBI TT'CG_AA
Cuts at: 4192 4192
Size: 5831
BstXI CCAn_nnnn'nTGG
Cuts at: 1455 2316 2823 1455
Size: 861 507 4463
Fragments arranged by size:
4463 861 507
BstYI r'GATC_y
Cuts at: 1 1356 1646 2107 2856 3323 3346 3681 3927
Size: 1355 290 461 749 467 23 335 246
Cuts at: 3927 4939 5025 5037 5383 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1355 1012 749 467 461 449 346 335
290 246 86 23 12
BtgI C'CryG_G
Cuts at: 2024 2816 2024
Size: 792 5039
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3781 3962 4125 3781
Size: 181 163 5487
Fragments arranged by size:
5487 181 163
BtsI GCAGTG_nn'
Cuts at: 919 2402 2727 3074 919
Size: 1483 325 347 3676
Fragments arranged by size:
3676 1483 347 325
ClaI AT'CG_AT
Cuts at: 2773 2773
Size: 5831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 825 860 825
Size: 35 5796
DraI TTT'AAA
Cuts at: 2830 2830
Size: 5831
DrdI GACnn_nn'nnGTC
Cuts at: 3537 4395 3537
Size: 858 4973
EaeI y'GGCC_r
Cuts at: 896 2009 3416 3590 3981 4008 5353 896
Size: 1113 1407 174 391 27 1345 1374
Fragments arranged by size:
1407 1374 1345 1113 391 174 27
EagI C'GGCC_G
Cuts at: 3416 3416
Size: 5831
EarI CTCTTCn'nnn_
Cuts at: 952 1554 1774 1805 3854 4064 952
Size: 602 220 31 2049 210 2719
Fragments arranged by size:
2719 2049 602 220 210 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4039 4349 4495 5641 4039
Size: 310 146 1146 4229
Fragments arranged by size:
4229 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1126 2843 1126
Size: 1717 4114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 703 1495 1770 2109 2281 3656 4088 4835
Size: 243 792 275 339 172 1375 432 747
Cuts at: 4835 5103 460
Size: 268 1188
Fragments arranged by size:
1375 1188 792 747 432 339 275 268
243 172
EcoO109I rG'GnC_Cy
Cuts at: 413 414 678 1931 2061 2970 413
Size: 1 264 1253 130 909 3274
Fragments arranged by size:
3274 1253 909 264 130 1
EcoRI G'AATT_C
Cuts at: 2835 2835
Size: 5831
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2139 3850 2139
Size: 1711 4120
FspI TGC'GCA
Cuts at: 3612 3612
Size: 5831
HaeII r_GCGC'y
Cuts at: 851 2507 3265 3513 4535 5514 851
Size: 1656 758 248 1022 979 1168
Fragments arranged by size:
1656 1168 1022 979 758 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 1535 1567 2025 2057 2490 2522 3673
Size: 32 797 32 458 32 433 32 1151
Cuts at: 3673 3703 3705 3735 706
Size: 30 2 30 2802
Fragments arranged by size:
2802 1151 797 458 433 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 390 633 2870 390
Size: 243 2237 3351
Fragments arranged by size:
3351 2237 243
KasI G'GCGC_C
Cuts at: 847 3261 3509 847
Size: 2414 248 3169
Fragments arranged by size:
3169 2414 248
KpnI G_GTAC'C
Cuts at: 2851 2851
Size: 5831
MluI A'CGCG_T
Cuts at: 5264 5264
Size: 5831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4502 4686 4502
Size: 184 5647
MscI TGG'CCA
Cuts at: 3592 3592
Size: 5831
MslI CAynn'nnrTG
Cuts at: 946 1753 2314 3820 3947 946
Size: 807 561 1506 127 2830
Fragments arranged by size:
2830 1506 807 561 127
MspA1I CmG'CkG
Cuts at: 480 601 870 1090 2292 2474 2696 2714 2942
Size: 121 269 220 1202 182 222 18 228
Cuts at: 2942 3256 3616 4629 4874 480
Size: 314 360 1013 245 1437
Fragments arranged by size:
1437 1202 1013 360 314 269 245 228
222 220 182 121 18
NaeI GCC'GGC
Cuts at: 4012 4012
Size: 5831
NarI GG'CG_CC
Cuts at: 848 3262 3510 848
Size: 2414 248 3169
Fragments arranged by size:
3169 2414 248
NgoMIV G'CCGG_C
Cuts at: 4010 4010
Size: 5831
NspI r_CATG'y
Cuts at: 226 396 2884 3384 4291 226
Size: 170 2488 500 907 1766
Fragments arranged by size:
2488 1766 907 500 170
PasI CC'CwG_GG
Cuts at: 1097 1890 1097
Size: 793 5038
PciI A'CATG_T
Cuts at: 4287 4287
Size: 5831
PfoI T'CCnGG_A
Cuts at: 590 1601 2258 590
Size: 1011 657 4163
Fragments arranged by size:
4163 1011 657
PmeI GTTT'AAAC
Cuts at: 2830 2830
Size: 5831
PmlI CAC'GTG
Cuts at: 1209 1455 1209
Size: 246 5585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2778 2810 4970 5002 2778
Size: 32 2160 32 3607
Fragments arranged by size:
3607 2160 32 32
PpuMI rG'GwC_Cy
Cuts at: 678 1931 2061 678
Size: 1253 130 4448
Fragments arranged by size:
4448 1253 130
PshAI GACnn'nnGTC
Cuts at: 628 628
Size: 5831
PsiI TTA'TAA
Cuts at: 757 757
Size: 5831
PspOMI G'GGCC_C
Cuts at: 413 413
Size: 5831
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1315 1347 1315
Size: 32 5799
PstI C_TGCA'G
Cuts at: 497 584 1133 1281 2878 3563 497
Size: 87 549 148 1597 685 2765
Fragments arranged by size:
2765 1597 685 549 148 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5831
PvuII CAG'CTG
Cuts at: 480 601 870 1090 3256 3616 480
Size: 121 269 220 2166 360 2695
Fragments arranged by size:
2695 2166 360 269 220 121
RsrII CG'GwC_CG
Cuts at: 4026 4026
Size: 5831
SacI G_AGCT'C
Cuts at: 1128 2845 1128
Size: 1717 4114
SalI G'TCGA_C
Cuts at: 2868 2868
Size: 5831
SapI GCTCTTCn'nnn_
Cuts at: 1554 3854 4064 1554
Size: 2300 210 3321
Fragments arranged by size:
3321 2300 210
SbfI CC_TGCA'GG
Cuts at: 2878 2878
Size: 5831
ScaI AGT'ACT
Cuts at: 5348 5348
Size: 5831
SfcI C'TryA_G
Cuts at: 33 321 493 580 1129 1277 2874 3559 4552
Size: 288 172 87 549 148 1597 685 993
Cuts at: 4552 4743 33
Size: 191 1121
Fragments arranged by size:
1597 1121 993 685 549 288 191 172
148 87
SfoI GGC'GCC
Cuts at: 849 3263 3511 849
Size: 2414 248 3169
Fragments arranged by size:
3169 2414 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
SmaI CCC'GGG
Cuts at: 2853 2853
Size: 5831
SmlI C'TyrA_G
Cuts at: 932 1372 2986 3901 4393 4655 932
Size: 440 1614 915 492 262 2108
Fragments arranged by size:
2108 1614 915 492 440 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5831
SphI G_CATG'C
Cuts at: 2884 3384 2884
Size: 500 5331
StuI AGG'CCT
Cuts at: 1525 1525
Size: 5831
StyI C'CwwG_G
Cuts at: 144 1104 2360 2489 2807 2965 3319 144
Size: 960 1256 129 318 158 354 2656
Fragments arranged by size:
2656 1256 960 354 318 158 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2373 3414 3547 4006 4215 2373
Size: 1041 133 459 209 3989
Fragments arranged by size:
3989 1041 459 209 133
TatI w'GTAC_w
Cuts at: 968 2279 5346 968
Size: 1311 3067 1453
Fragments arranged by size:
3067 1453 1311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 909 941 2286 2318 5348 5380 909
Size: 32 1345 32 3030 32 1360
Fragments arranged by size:
3030 1360 1345 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3628 3628
Size: 5831
XbaI T'CTAG_A
Cuts at: 2862 2862
Size: 5831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1094 3326 1094
Size: 2232 3599
XhoI C'TCGA_G
Cuts at: 932 932
Size: 5831
XmaI C'CCGG_G
Cuts at: 2851 2851
Size: 5831
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BaeI BfrBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI