Restriction map for
pF1KB8525
(Circular) (Six-base) MAPSORT of: pF1KB8525.seq Check: 5430 from: 1 to: 5996
pF1KB8525 5996 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 2363 2363
Size: 5996
AccI GT'mk_AC
Cuts at: 3034 3034
Size: 5996
Acc65I G'GTAC_C
Cuts at: 233 3012 233
Size: 2779 3217
AclI AA'CG_TT
Cuts at: 5836 5836
Size: 5996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 1235 1717 3821 4253 5000 5268 1189
Size: 46 482 2104 432 747 268 1917
Fragments arranged by size:
2104 1917 747 482 432 268 46
AflIII A'CryG_T
Cuts at: 1959 3348 4452 5429 1959
Size: 1389 1104 977 2526
Fragments arranged by size:
2526 1389 1104 977
AgeI A'CCGG_T
Cuts at: 4384 4384
Size: 5996
AleI CACnn'nnGTG
Cuts at: 862 862
Size: 5996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2568 2568
Size: 5996
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1639 1671 1639
Size: 32 5964
AlwNI CAG_nnn'CTG
Cuts at: 392 1842 3301 4868 392
Size: 1450 1459 1567 1520
Fragments arranged by size:
1567 1520 1459 1450
ApaI G_GGCC'C
Cuts at: 335 335
Size: 5996
ApaLI G'TGCA_C
Cuts at: 4766 4766
Size: 5996
ApoI r'AATT_y
Cuts at: 1441 3000 5349 5605 1441
Size: 1559 2349 256 1832
Fragments arranged by size:
2349 1832 1559 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5996
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5996
AvaI C'yCGr_G
Cuts at: 150 248 482 2693 3016 150
Size: 98 234 2211 323 3130
Fragments arranged by size:
3130 2211 323 234 98
BamHI G'GATC_C
Cuts at: 3021 3021
Size: 5996
BanI G'GyrC_C
Cuts at: 129 233 1506 2882 3012 3426 3674 3709 129
Size: 104 1273 1376 130 414 248 35 2416
Fragments arranged by size:
2416 1376 1273 414 248 130 104 35
BanII G_rGCy'C
Cuts at: 335 483 740 2054 2942 3010 4040 335
Size: 148 257 1314 888 68 1030 2291
Fragments arranged by size:
2291 1314 1030 888 257 148 68
BbeI G_GCGC'C
Cuts at: 133 2886 3430 3678 133
Size: 2753 544 248 2451
Fragments arranged by size:
2753 2451 544 248
BbsI GAAGACnn'nnnn_
Cuts at: 1053 2031 2172 2221 2737 1053
Size: 978 141 49 516 4312
Fragments arranged by size:
4312 978 516 141 49
BbvCI CC'TCA_GC
Cuts at: 869 869
Size: 5996
BciVI GTATCCnnnnn_n'
Cuts at: 3894 4655 5996 3894
Size: 761 1341 3894
Fragments arranged by size:
3894 1341 761
BclI T'GATC_A
Cuts at: 2508 2687 3516 2508
Size: 179 829 4988
Fragments arranged by size:
4988 829 179
BglI GCCn_nnn'nGGC
Cuts at: 152 1531 152
Size: 1379 4617
BglII A'GATC_T
Cuts at: 1313 3511 5548 1313
Size: 2198 2037 1761
Fragments arranged by size:
2198 2037 1761
BlpI GC'TnA_GC
Cuts at: 2953 3108 2953
Size: 155 5841
Bme1580I G_kGCm'C
Cuts at: 335 630 1157 2672 3621 3714 4770 335
Size: 295 527 1515 949 93 1056 1561
Fragments arranged by size:
1561 1515 1056 949 527 295 93
BmgBI CAC'GTC
Cuts at: 702 702
Size: 5996
BmrI ACTGGGnnnn_n'
Cuts at: 3208 3289 3624 5768 3208
Size: 81 335 2144 3436
Fragments arranged by size:
3436 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1686 2234 2850 4363 1686
Size: 548 616 1513 3319
Fragments arranged by size:
3319 1513 616 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 897 1105 1583 1612 1887 2379 2401 2839
Size: 182 208 478 29 275 492 22 438
Cuts at: 2839 715
Size: 3872
Fragments arranged by size:
3872 492 478 438 275 208 182 29
22
Bpu10I CC'TnA_GC
Cuts at: 869 2936 869
Size: 2067 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 3172 4051 4543 4841 724
Size: 2448 879 492 298 1879
Fragments arranged by size:
2448 1879 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2470 5700 2470
Size: 3230 2766
BsaAI yAC'GTr
Cuts at: 3349 3349
Size: 5996
BsaBI GATnn'nnATC
Cuts at: 6 3510 5547 6
Size: 3504 2037 455
Fragments arranged by size:
3504 2037 455
BsaHI Gr'CG_yC
Cuts at: 130 2360 2883 3427 3675 4377 5873 130
Size: 2230 523 544 248 702 1496 253
Fragments arranged by size:
2230 1496 702 544 523 253 248
BsaWI w'CCGG_w
Cuts at: 494 2312 3185 3706 4384 4658 4805 5216 494
Size: 1818 873 521 678 274 147 411 1274
Fragments arranged by size:
1818 1274 873 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 125 155 470 500 525 555 2752 2782 125
Size: 30 315 30 25 30 2197 30 3339
Fragments arranged by size:
3339 2197 315 30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1498 1580 2628 2773 2776 2839 1498
Size: 82 1048 145 3 63 4655
Fragments arranged by size:
4655 1048 145 82 63 3
BseYI C'CCAG_C
Cuts at: 385 465 1827 2375 2454 3421 4179 4756 5252
Size: 80 1362 548 79 967 758 577 496
Cuts at: 5252 385
Size: 1129
Fragments arranged by size:
1362 1129 967 758 577 548 496 80
79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1320 2293 1320
Size: 973 5023
BsiEI CG_ry'CG
Cuts at: 104 1575 2949 3538 3584 4792 104
Size: 1471 1374 589 46 1208 1308
Fragments arranged by size:
1471 1374 1308 1208 589 46
BsiHKAI G_wGCw'C
Cuts at: 562 740 2663 3010 3788 3978 4770 562
Size: 178 1923 347 778 190 792 1788
Fragments arranged by size:
1923 1788 792 778 347 190 178
BsmBI CGTCTCn'nnnn_
Cuts at: 1258 1896 2367 1258
Size: 638 471 4887
Fragments arranged by size:
4887 638 471
Bsp1286I G_dGCh'C
Cuts at: 335 483 562 630 740 1157 2054 2663 2672
Size: 148 79 68 110 417 897 609 9
Cuts at: 2672 2942 3010 3621 3714 3788 3978 4040 4770
Size: 270 68 611 93 74 190 62 730
Cuts at: 4770 335
Size: 1561
Fragments arranged by size:
1561 897 730 611 609 417 270 190
148 110 93 79 74 68 68 62
9
BspEI T'CCGG_A
Cuts at: 5216 5216
Size: 5996
BspHI T'CATG_A
Cuts at: 5172 5172
Size: 5996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3046 3260 3562 3943 3046
Size: 214 302 381 5099
Fragments arranged by size:
5099 381 302 214
BsrBI CCG'CTC
Cuts at: 50 644 2876 4288 4342 5826 5991 50
Size: 594 2232 1412 54 1484 165 55
Fragments arranged by size:
2232 1484 1412 594 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1649 2385 2810 3908 1649
Size: 736 425 1098 3737
Fragments arranged by size:
3737 1098 736 425
BsrFI r'CCGG_y
Cuts at: 935 2948 3994 4175 4384 935
Size: 2013 1046 181 209 2547
Fragments arranged by size:
2547 2013 1046 209 181
BsrGI T'GTAC_A
Cuts at: 617 1241 617
Size: 624 5372
BssHII G'CGCG_C
Cuts at: 609 609
Size: 5996
BssSI C'ACGA_G
Cuts at: 4267 4625 4267
Size: 358 5638
BstAPI GCAn_nnn'nTGC
Cuts at: 2608 3373 2608
Size: 765 5231
BstBI TT'CG_AA
Cuts at: 4357 4357
Size: 5996
BstEII G'GTnAC_C
Cuts at: 2059 2059
Size: 5996
BstXI CCAn_nnnn'nTGG
Cuts at: 864 864
Size: 5996
BstYI r'GATC_y
Cuts at: 1 1143 1313 3021 3488 3511 3846 4092 5104
Size: 1142 170 1708 467 23 335 246 1012
Cuts at: 5104 5190 5202 5548 1
Size: 86 12 346 449
Fragments arranged by size:
1708 1142 1012 467 449 346 335 246
170 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1481 2345 1481
Size: 864 5132
BtgI C'CryG_G
Cuts at: 677 677
Size: 5996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3946 4127 4290 3946
Size: 181 163 5652
Fragments arranged by size:
5652 181 163
BtsI GCAGTG_nn'
Cuts at: 573 620 2561 3239 573
Size: 47 1941 678 3330
Fragments arranged by size:
3330 1941 678 47
DraI TTT'AAA
Cuts at: 2995 2995
Size: 5996
DrdI GACnn_nn'nnGTC
Cuts at: 3702 4560 3702
Size: 858 5138
EaeI y'GGCC_r
Cuts at: 353 652 1572 1674 3581 3755 4146 4173 5518
Size: 299 920 102 1907 174 391 27 1345
Cuts at: 5518 353
Size: 831
Fragments arranged by size:
1907 1345 920 831 391 299 174 102
27
EagI C'GGCC_G
Cuts at: 1572 3581 1572
Size: 2009 3987
EarI CTCTTCn'nnn_
Cuts at: 142 1656 4019 4229 142
Size: 1514 2363 210 1909
Fragments arranged by size:
2363 1909 1514 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 1114 4204 4514 4660 5806 133
Size: 981 3090 310 146 1146 323
Fragments arranged by size:
3090 1146 981 323 310 146
EcoICRI GAG'CTC
Cuts at: 738 3008 738
Size: 2270 3726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 897 1105 1189 1235 1583 1612 1717 1887
Size: 182 208 84 46 348 29 105 170
Cuts at: 1887 2379 2401 2839 3821 4253 5000 5268 715
Size: 492 22 438 982 432 747 268 1443
Fragments arranged by size:
1443 982 747 492 438 432 348 268
208 182 170 105 84 46 29 22
EcoO109I rG'GnC_Cy
Cuts at: 240 331 459 1820 2916 2929 3135 240
Size: 91 128 1361 1096 13 206 3101
Fragments arranged by size:
3101 1361 1096 206 128 91 13
EcoRI G'AATT_C
Cuts at: 3000 3000
Size: 5996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4015 4015
Size: 5996
FspI TGC'GCA
Cuts at: 2680 3777 2680
Size: 1097 4899
HaeII r_GCGC'y
Cuts at: 133 694 1176 2886 3430 3678 4700 5679 133
Size: 561 482 1710 544 248 1022 979 450
Fragments arranged by size:
1710 1022 979 561 544 482 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2493 2525 2752 2784 3838 3868 3870 3900 2493
Size: 32 227 32 1054 30 2 30 4589
Fragments arranged by size:
4589 1054 227 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3035 3035
Size: 5996
KasI G'GCGC_C
Cuts at: 129 2882 3426 3674 129
Size: 2753 544 248 2451
Fragments arranged by size:
2753 2451 544 248
KpnI G_GTAC'C
Cuts at: 237 3016 237
Size: 2779 3217
MfeI C'AATT_G
Cuts at: 1302 1302
Size: 5996
MluI A'CGCG_T
Cuts at: 5429 5429
Size: 5996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1448 2690 2759 4667 4851 1448
Size: 1242 69 1908 184 2593
Fragments arranged by size:
2593 1908 1242 184 69
MscI TGG'CCA
Cuts at: 355 654 1676 3757 355
Size: 299 1022 2081 2594
Fragments arranged by size:
2594 2081 1022 299
MslI CAynn'nnrTG
Cuts at: 832 862 3985 4112 832
Size: 30 3123 127 2716
Fragments arranged by size:
3123 2716 127 30
MspA1I CmG'CkG
Cuts at: 392 679 1150 1512 1697 1831 2379 3107 3421
Size: 287 471 362 185 134 548 728 314
Cuts at: 3421 3781 4794 5039 392
Size: 360 1013 245 1349
Fragments arranged by size:
1349 1013 728 548 471 362 360 314
287 245 185 134
NaeI GCC'GGC
Cuts at: 4177 4177
Size: 5996
NarI GG'CG_CC
Cuts at: 130 2883 3427 3675 130
Size: 2753 544 248 2451
Fragments arranged by size:
2753 2451 544 248
NgoMIV G'CCGG_C
Cuts at: 4175 4175
Size: 5996
NspI r_CATG'y
Cuts at: 1963 3049 3549 4456 1963
Size: 1086 500 907 3503
Fragments arranged by size:
3503 1086 907 500
PasI CC'CwG_GG
Cuts at: 215 2046 2924 215
Size: 1831 878 3287
Fragments arranged by size:
3287 1831 878
PciI A'CATG_T
Cuts at: 1959 4452 1959
Size: 2493 3503
PflMI CCAn_nnn'nTGG
Cuts at: 519 1842 2162 519
Size: 1323 320 4353
Fragments arranged by size:
4353 1323 320
PfoI T'CCnGG_A
Cuts at: 364 529 1499 2395 364
Size: 165 970 896 3965
Fragments arranged by size:
3965 970 896 165
PmeI GTTT'AAAC
Cuts at: 2995 2995
Size: 5996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5135 5167 5135
Size: 32 5964
PpuMI rG'GwC_Cy
Cuts at: 459 1820 2929 459
Size: 1361 1109 3526
Fragments arranged by size:
3526 1361 1109
PshAI GACnn'nnGTC
Cuts at: 1843 1843
Size: 5996
PsiI TTA'TAA
Cuts at: 2987 2987
Size: 5996
PspOMI G'GGCC_C
Cuts at: 331 331
Size: 5996
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1261 1293 1261
Size: 32 5964
PstI C_TGCA'G
Cuts at: 847 3043 3728 847
Size: 2196 685 3115
Fragments arranged by size:
3115 2196 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5996
PvuII CAG'CTG
Cuts at: 1150 1831 2379 3421 3781 1150
Size: 681 548 1042 360 3365
Fragments arranged by size:
3365 1042 681 548 360
RsrII CG'GwC_CG
Cuts at: 4191 4191
Size: 5996
SacI G_AGCT'C
Cuts at: 740 3010 740
Size: 2270 3726
SacII CC_GC'GG
Cuts at: 680 680
Size: 5996
SalI G'TCGA_C
Cuts at: 3033 3033
Size: 5996
SanDI GG'GwC_CC
Cuts at: 1820 2929 1820
Size: 1109 4887
SapI GCTCTTCn'nnn_
Cuts at: 4019 4229 4019
Size: 210 5786
SbfI CC_TGCA'GG
Cuts at: 3043 3043
Size: 5996
ScaI AGT'ACT
Cuts at: 5513 5513
Size: 5996
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 5996
SfcI C'TryA_G
Cuts at: 33 540 843 3039 3724 4717 4908 33
Size: 507 303 2196 685 993 191 1121
Fragments arranged by size:
2196 1121 993 685 507 303 191
SfoI GGC'GCC
Cuts at: 131 2884 3428 3676 131
Size: 2753 544 248 2451
Fragments arranged by size:
2753 2451 544 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5996
SmaI CCC'GGG
Cuts at: 152 484 3018 152
Size: 332 2534 3130
Fragments arranged by size:
3130 2534 332
SmlI C'TyrA_G
Cuts at: 739 3151 4066 4558 4820 739
Size: 2412 915 492 262 1915
Fragments arranged by size:
2412 1915 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5996
SphI G_CATG'C
Cuts at: 3049 3549 3049
Size: 500 5496
StuI AGG'CCT
Cuts at: 667 667
Size: 5996
StyI C'CwwG_G
Cuts at: 335 1535 1775 2333 3130 3484 335
Size: 1200 240 558 797 354 2847
Fragments arranged by size:
2847 1200 797 558 354 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 399 983 1277 2903 3579 3712 4171 4380 399
Size: 584 294 1626 676 133 459 209 2015
Fragments arranged by size:
2015 1626 676 584 459 294 209 133
TatI w'GTAC_w
Cuts at: 617 1241 5511 617
Size: 624 4270 1102
Fragments arranged by size:
4270 1102 624
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 470 481 502 513 5513 5545 125
Size: 32 313 11 21 11 5000 32 576
Fragments arranged by size:
5000 576 313 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 2736 3793 2736
Size: 1057 4939
XbaI T'CTAG_A
Cuts at: 3027 3027
Size: 5996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1533 3491 1533
Size: 1958 4038
XmaI C'CCGG_G
Cuts at: 150 482 3016 150
Size: 332 2534 3130
Fragments arranged by size:
3130 2534 332
XmnI GAAnn'nnTTC
Cuts at: 442 442
Size: 5996
ZraI GAC'GTC
Cuts at: 2361 2361
Size: 5996
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PspXI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI