Restriction map for
pF1KB8523
(Circular) (Six-base) MAPSORT of: pF1KB8523.seq Check: 3516 from: 1 to: 5924
pF1KB8523 5924 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 884 884
Size: 5924
AatII G_ACGT'C
Cuts at: 2432 2432
Size: 5924
AccI GT'mk_AC
Cuts at: 704 2731 2962 704
Size: 2027 231 3666
Fragments arranged by size:
3666 2027 231
Acc65I G'GTAC_C
Cuts at: 2940 2940
Size: 5924
AclI AA'CG_TT
Cuts at: 5764 5764
Size: 5924
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 2747 3749 4181 4928 5196 2078
Size: 669 1002 432 747 268 2806
Fragments arranged by size:
2806 1002 747 669 432 268
AfeI AGC'GCT
Cuts at: 2863 2863
Size: 5924
AflIII A'CryG_T
Cuts at: 827 1647 1672 1871 2327 3276 4380 5357 827
Size: 820 25 199 456 949 1104 977 1394
Fragments arranged by size:
1394 1104 977 949 820 456 199 25
AgeI A'CCGG_T
Cuts at: 4312 4312
Size: 5924
AhdI GACnn_n'nnGTC
Cuts at: 154 154
Size: 5924
AleI CACnn'nnGTG
Cuts at: 391 974 1097 1232 1712 1847 1970 2380 391
Size: 583 123 135 480 135 123 410 3935
Fragments arranged by size:
3935 583 480 410 135 135 123 123
AlwNI CAG_nnn'CTG
Cuts at: 951 3229 4796 951
Size: 2278 1567 2079
Fragments arranged by size:
2278 2079 1567
ApaI G_GGCC'C
Cuts at: 570 570
Size: 5924
ApaLI G'TGCA_C
Cuts at: 816 1182 1325 1448 4694 816
Size: 366 143 123 3246 2046
Fragments arranged by size:
3246 2046 366 143 123
ApoI r'AATT_y
Cuts at: 1986 2115 2928 5277 5533 1986
Size: 129 813 2349 256 2377
Fragments arranged by size:
2377 2349 813 256 129
AseI AT'TA_AT
Cuts at: 20 20
Size: 5924
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
AvaI C'yCGr_G
Cuts at: 389 1556 2183 2944 389
Size: 1167 627 761 3369
Fragments arranged by size:
3369 1167 761 627
BamHI G'GATC_C
Cuts at: 2092 2949 2092
Size: 857 5067
BanI G'GyrC_C
Cuts at: 505 2382 2940 3354 3602 3637 505
Size: 1877 558 414 248 35 2792
Fragments arranged by size:
2792 1877 558 414 248 35
BanII G_rGCy'C
Cuts at: 570 740 1006 2302 2421 2534 2938 3968 570
Size: 170 266 1296 119 113 404 1030 2526
Fragments arranged by size:
2526 1296 1030 404 266 170 119 113
BbeI G_GCGC'C
Cuts at: 3358 3606 3358
Size: 248 5676
BbsI GAAGACnn'nnnn_
Cuts at: 747 1675 2043 2630 2643 2720 747
Size: 928 368 587 13 77 3951
Fragments arranged by size:
3951 928 587 368 77 13
BbvCI CC'TCA_GC
Cuts at: 1043 1904 2027 1043
Size: 861 123 4940
Fragments arranged by size:
4940 861 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 631 665 631
Size: 34 5890
BciVI GTATCCnnnnn_n'
Cuts at: 3822 4583 5924 3822
Size: 761 1341 3822
Fragments arranged by size:
3822 1341 761
BclI T'GATC_A
Cuts at: 3444 3444
Size: 5924
BglI GCCn_nnn'nGGC
Cuts at: 646 1274 1397 646
Size: 628 123 5173
Fragments arranged by size:
5173 628 123
BglII A'GATC_T
Cuts at: 3439 5476 3439
Size: 2037 3887
BlpI GC'TnA_GC
Cuts at: 3036 3036
Size: 5924
Bme1580I G_kGCm'C
Cuts at: 508 570 820 858 1186 1329 1452 2387 3549
Size: 62 250 38 328 143 123 935 1162
Cuts at: 3549 3642 4698 508
Size: 93 1056 1734
Fragments arranged by size:
1734 1162 1056 935 328 250 143 123
93 62 38
BmgBI CAC'GTC
Cuts at: 1673 1673
Size: 5924
BmrI ACTGGGnnnn_n'
Cuts at: 524 2318 3136 3217 3552 5696 524
Size: 1794 818 81 335 2144 752
Fragments arranged by size:
2144 1794 818 752 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2541 4291 2541
Size: 1750 4174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 2182 2633 268
Size: 1914 451 3559
Fragments arranged by size:
3559 1914 451
Bpu10I CC'TnA_GC
Cuts at: 653 1043 1904 2027 2420 653
Size: 390 861 123 393 4157
Fragments arranged by size:
4157 861 393 390 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 1528 2581 3100 3979 4471 4769 424
Size: 1104 1053 519 879 492 298 1579
Fragments arranged by size:
1579 1104 1053 879 519 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 615 5628 615
Size: 5013 911
BsaAI yAC'GTr
Cuts at: 190 1650 1874 3277 190
Size: 1460 224 1403 2837
Fragments arranged by size:
2837 1460 1403 224
BsaBI GATnn'nnATC
Cuts at: 6 3438 5475 6
Size: 3432 2037 455
Fragments arranged by size:
3432 2037 455
BsaHI Gr'CG_yC
Cuts at: 2429 3355 3603 4305 5801 2429
Size: 926 248 702 1496 2552
Fragments arranged by size:
2552 1496 926 702 248
BsaWI w'CCGG_w
Cuts at: 3113 3634 4312 4586 4733 5144 3113
Size: 521 678 274 147 411 3893
Fragments arranged by size:
3893 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1637 1667 1637
Size: 30 5894
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 1384 2518 151
Size: 1233 1134 3557
Fragments arranged by size:
3557 1233 1134
BseYI C'CCAG_C
Cuts at: 3349 4107 4684 5180 3349
Size: 758 577 496 4093
Fragments arranged by size:
4093 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 852
Size: 5924
BsiEI CG_ry'CG
Cuts at: 104 3466 3512 4720 104
Size: 3362 46 1208 1308
Fragments arranged by size:
3362 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 820 1186 1329 1452 1516 1655 1698 2938 3716
Size: 366 143 123 64 139 43 1240 778
Cuts at: 3716 3906 4698 820
Size: 190 792 2046
Fragments arranged by size:
2046 1240 792 778 366 190 143 139
123 64 43
BsmI GAATG_Cn'
Cuts at: 547 547
Size: 5924
BsmBI CGTCTCn'nnnn_
Cuts at: 778 2436 778
Size: 1658 4266
Bsp1286I G_dGCh'C
Cuts at: 508 570 740 820 858 1006 1186 1329 1452
Size: 62 170 80 38 148 180 143 123
Cuts at: 1452 1516 1655 1698 2302 2387 2421 2534 2938
Size: 64 139 43 604 85 34 113 404
Cuts at: 2938 3549 3642 3716 3906 3968 4698 508
Size: 611 93 74 190 62 730 1734
Fragments arranged by size:
1734 730 611 604 404 190 180 170
148 143 139 123 113 93 85 80
74 64 62 62 43 38 34
BspEI T'CCGG_A
Cuts at: 5144 5144
Size: 5924
BspHI T'CATG_A
Cuts at: 5100 5100
Size: 5924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 884 1312 1435 1558 1804 2974 3188 3490 3871
Size: 428 123 123 246 1170 214 302 381
Cuts at: 3871 884
Size: 2937
Fragments arranged by size:
2937 1170 428 381 302 246 214 123
123
BsrBI CCG'CTC
Cuts at: 50 183 2182 4216 4270 5754 5919 50
Size: 133 1999 2034 54 1484 165 55
Fragments arranged by size:
2034 1999 1484 165 133 55 54
BsrDI GCAATG_nn'
Cuts at: 3836 3836
Size: 5924
BsrFI r'CCGG_y
Cuts at: 3922 4103 4312 3922
Size: 181 209 5534
Fragments arranged by size:
5534 209 181
BssSI C'ACGA_G
Cuts at: 1228 4195 4553 1228
Size: 2967 358 2599
Fragments arranged by size:
2967 2599 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1427 2051 3301 1427
Size: 624 1250 4050
Fragments arranged by size:
4050 1250 624
BstBI TT'CG_AA
Cuts at: 4285 4285
Size: 5924
BstXI CCAn_nnnn'nTGG
Cuts at: 693 693
Size: 5924
BstYI r'GATC_y
Cuts at: 1 948 1618 2092 2949 3416 3439 3774 4020
Size: 947 670 474 857 467 23 335 246
Cuts at: 4020 5032 5118 5130 5476 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 947 857 670 474 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 577 626 1640 577
Size: 49 1014 4861
Fragments arranged by size:
4861 1014 49
BtgI C'CryG_G
Cuts at: 107 500 592 635 959 1092 1395 1461 1584
Size: 393 92 43 324 133 303 66 123
Cuts at: 1584 1707 1830 1953 2270 107
Size: 123 123 123 317 3761
Fragments arranged by size:
3761 393 324 317 303 133 123 123
123 123 92 66 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 528 1676 3874 4055 4218 528
Size: 1148 2198 181 163 2234
Fragments arranged by size:
2234 2198 1148 181 163
BtsI GCAGTG_nn'
Cuts at: 312 1263 1740 3167 312
Size: 951 477 1427 3069
Fragments arranged by size:
3069 1427 951 477
ClaI AT'CG_AT
Cuts at: 2087 2087
Size: 5924
DraI TTT'AAA
Cuts at: 2923 2923
Size: 5924
DrdI GACnn_nn'nnGTC
Cuts at: 3630 4488 3630
Size: 858 5066
EaeI y'GGCC_r
Cuts at: 521 638 764 3509 3683 4074 4101 5446 521
Size: 117 126 2745 174 391 27 1345 999
Fragments arranged by size:
2745 1345 999 391 174 126 117 27
EagI C'GGCC_G
Cuts at: 3509 3509
Size: 5924
EarI CTCTTCn'nnn_
Cuts at: 1048 2105 2554 3947 4157 1048
Size: 1057 449 1393 210 2815
Fragments arranged by size:
2815 1393 1057 449 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4132 4442 4588 5734 4132
Size: 310 146 1146 4322
Fragments arranged by size:
4322 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2936 2936
Size: 5924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 2078 2182 2633 2747 3749 4181 4928 5196
Size: 1810 104 451 114 1002 432 747 268
Cuts at: 5196 268
Size: 996
Fragments arranged by size:
1810 1002 996 747 451 432 268 114
104
EcoNI CCTnn'n_nnAGG
Cuts at: 450 1042 1308 1800 450
Size: 592 266 492 4574
Fragments arranged by size:
4574 592 492 266
EcoO109I rG'GnC_Cy
Cuts at: 566 1539 2366 3063 566
Size: 973 827 697 3427
Fragments arranged by size:
3427 973 827 697
EcoRI G'AATT_C
Cuts at: 1986 2928 1986
Size: 942 4982
EcoRV GAT'ATC
Cuts at: 2701 2701
Size: 5924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2565 2737 3943 2565
Size: 172 1206 4546
Fragments arranged by size:
4546 1206 172
FspI TGC'GCA
Cuts at: 3705 3705
Size: 5924
HaeII r_GCGC'y
Cuts at: 2865 3358 3606 4628 5607 2865
Size: 493 248 1022 979 3182
Fragments arranged by size:
3182 1022 979 493 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 477 509 1637 1669 1883 1915 2416
Size: 32 305 32 1128 32 214 32 501
Cuts at: 2416 2448 3766 3796 3798 3828 140
Size: 32 1318 30 2 30 2236
Fragments arranged by size:
2236 1318 1128 501 305 214 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 312 1239 1362 1608 2963 312
Size: 927 123 246 1355 3273
Fragments arranged by size:
3273 1355 927 246 123
HindIII A'AGCT_T
Cuts at: 2740 2740
Size: 5924
HpaI GTT'AAC
Cuts at: 1239 1239
Size: 5924
KasI G'GCGC_C
Cuts at: 3354 3602 3354
Size: 248 5676
KpnI G_GTAC'C
Cuts at: 2944 2944
Size: 5924
MfeI C'AATT_G
Cuts at: 2066 2066
Size: 5924
MluI A'CGCG_T
Cuts at: 5357 5357
Size: 5924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2700 4595 4779 2700
Size: 1895 184 3845
Fragments arranged by size:
3845 1895 184
MscI TGG'CCA
Cuts at: 523 766 3685 523
Size: 243 2919 2762
Fragments arranged by size:
2919 2762 243
MslI CAynn'nnrTG
Cuts at: 323 391 691 851 974 1097 1109 1232 1466
Size: 68 300 160 123 123 12 123 234
Cuts at: 1466 1589 1712 1724 1835 1847 1849 1872 1970
Size: 123 123 12 111 12 2 23 98
Cuts at: 1970 2380 3913 4040 323
Size: 410 1533 127 2207
Fragments arranged by size:
2207 1533 410 300 234 160 127 123
123 123 123 123 111 98 68 23
12 12 12 2
MspA1I CmG'CkG
Cuts at: 1388 1476 3035 3349 3709 4722 4967 1388
Size: 88 1559 314 360 1013 245 2345
Fragments arranged by size:
2345 1559 1013 360 314 245 88
NaeI GCC'GGC
Cuts at: 4105 4105
Size: 5924
NarI GG'CG_CC
Cuts at: 3355 3603 3355
Size: 248 5676
NcoI C'CATG_G
Cuts at: 107 959 1461 1584 1830 1953 107
Size: 852 502 123 246 123 4078
Fragments arranged by size:
4078 852 502 246 123 123
NgoMIV G'CCGG_C
Cuts at: 4103 4103
Size: 5924
NspI r_CATG'y
Cuts at: 831 2331 2977 3477 4384 831
Size: 1500 646 500 907 2371
Fragments arranged by size:
2371 1500 907 646 500
PciI A'CATG_T
Cuts at: 827 2327 4380 827
Size: 1500 2053 2371
Fragments arranged by size:
2371 2053 1500
PfoI T'CCnGG_A
Cuts at: 944 944
Size: 5924
PmeI GTTT'AAAC
Cuts at: 2923 2923
Size: 5924
PmlI CAC'GTG
Cuts at: 190 1650 1874 190
Size: 1460 224 4240
Fragments arranged by size:
4240 1460 224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 5063
Size: 32 5892
PpuMI rG'GwC_Cy
Cuts at: 1539 1539
Size: 5924
PshAI GACnn'nnGTC
Cuts at: 374 374
Size: 5924
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5924
PspOMI G'GGCC_C
Cuts at: 566 566
Size: 5924
PspXI vC'TCGA_Gb
Cuts at: 2183 2183
Size: 5924
PstI C_TGCA'G
Cuts at: 895 1309 1432 1801 2971 3656 895
Size: 414 123 369 1170 685 3163
Fragments arranged by size:
3163 1170 685 414 369 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5924
PvuII CAG'CTG
Cuts at: 1476 3349 3709 1476
Size: 1873 360 3691
Fragments arranged by size:
3691 1873 360
RsrII CG'GwC_CG
Cuts at: 4119 4119
Size: 5924
SacI G_AGCT'C
Cuts at: 2938 2938
Size: 5924
SalI G'TCGA_C
Cuts at: 2961 2961
Size: 5924
SapI GCTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
SbfI CC_TGCA'GG
Cuts at: 2971 2971
Size: 5924
ScaI AGT'ACT
Cuts at: 5441 5441
Size: 5924
SexAI A'CCwGG_T
Cuts at: 278 2072 278
Size: 1794 4130
SfcI C'TryA_G
Cuts at: 33 891 1023 1305 1428 1797 1823 2967 3652
Size: 858 132 282 123 369 26 1144 685
Cuts at: 3652 4645 4836 33
Size: 993 191 1121
Fragments arranged by size:
1144 1121 993 858 685 369 282 191
132 123 26
SfoI GGC'GCC
Cuts at: 3356 3604 3356
Size: 248 5676
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
SmaI CCC'GGG
Cuts at: 1558 2946 1558
Size: 1388 4536
SmlI C'TyrA_G
Cuts at: 439 1543 2183 2596 3079 3994 4486 4748 439
Size: 1104 640 413 483 915 492 262 1615
Fragments arranged by size:
1615 1104 915 640 492 483 413 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5924
SphI G_CATG'C
Cuts at: 2977 3477 2977
Size: 500 5424
StyI C'CwwG_G
Cuts at: 107 959 1461 1584 1830 1953 2349 2453 3058
Size: 852 502 123 246 123 396 104 605
Cuts at: 3058 3412 107
Size: 354 2619
Fragments arranged by size:
2619 852 605 502 396 354 246 123
123 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 331 3507 3640 4099 4308 331
Size: 3176 133 459 209 1947
Fragments arranged by size:
3176 1947 459 209 133
TatI w'GTAC_w
Cuts at: 5439 5439
Size: 5924
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1044 1076 1659 1691 5441 5473 1044
Size: 32 583 32 3750 32 1495
Fragments arranged by size:
3750 1495 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 751 2074 3721 751
Size: 1323 1647 2954
Fragments arranged by size:
2954 1647 1323
XbaI T'CTAG_A
Cuts at: 2619 2955 2619
Size: 336 5588
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 516 1024 3419 516
Size: 508 2395 3021
Fragments arranged by size:
3021 2395 508
XhoI C'TCGA_G
Cuts at: 2183 2183
Size: 5924
XmaI C'CCGG_G
Cuts at: 1556 2944 1556
Size: 1388 4536
XmnI GAAnn'nnTTC
Cuts at: 430 430
Size: 5924
ZraI GAC'GTC
Cuts at: 2430 2430
Size: 5924
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AlfI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI
FspAI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI