Restriction map for
pF1KB8522
(Circular) (Six-base) MAPSORT of: pF1KB8522.seq Check: 9211 from: 1 to: 5750
pF1KB8522 5750 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 2257 2788 2257
Size: 531 5219
Acc65I G'GTAC_C
Cuts at: 2766 2766
Size: 5750
AclI AA'CG_TT
Cuts at: 5590 5590
Size: 5750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 3575 4007 4754 5022 290
Size: 3285 432 747 268 1018
Fragments arranged by size:
3285 1018 747 432 268
AflIII A'CryG_T
Cuts at: 400 743 1508 3102 4206 5183 400
Size: 343 765 1594 1104 977 967
Fragments arranged by size:
1594 1104 977 967 765 343
AgeI A'CCGG_T
Cuts at: 4138 4138
Size: 5750
AleI CACnn'nnGTG
Cuts at: 997 2689 997
Size: 1692 4058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2707 2707
Size: 5750
AlwNI CAG_nnn'CTG
Cuts at: 3055 4622 3055
Size: 1567 4183
ApaLI G'TGCA_C
Cuts at: 4520 4520
Size: 5750
ApoI r'AATT_y
Cuts at: 2149 2397 2754 5103 5359 2149
Size: 248 357 2349 256 2540
Fragments arranged by size:
2540 2349 357 256 248
AseI AT'TA_AT
Cuts at: 20 20
Size: 5750
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5750
AvaI C'yCGr_G
Cuts at: 2044 2770 2044
Size: 726 5024
BamHI G'GATC_C
Cuts at: 496 2775 496
Size: 2279 3471
BanI G'GyrC_C
Cuts at: 1063 2245 2766 3180 3428 3463 1063
Size: 1182 521 414 248 35 3350
Fragments arranged by size:
3350 1182 521 414 248 35
BanII G_rGCy'C
Cuts at: 959 2764 3794 959
Size: 1805 1030 2915
Fragments arranged by size:
2915 1805 1030
BbeI G_GCGC'C
Cuts at: 1067 3184 3432 1067
Size: 2117 248 3385
Fragments arranged by size:
3385 2117 248
BbsI GAAGACnn'nnnn_
Cuts at: 299 1832 299
Size: 1533 4217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1627 1661 1931 1965 1627
Size: 34 270 34 5412
Fragments arranged by size:
5412 270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 390 405 3648 4409 5750 390
Size: 15 3243 761 1341 390
Fragments arranged by size:
3243 1341 761 390 15
BclI T'GATC_A
Cuts at: 3270 3270
Size: 5750
BfrBI ATG'CAT
Cuts at: 421 1883 421
Size: 1462 4288
BglI GCCn_nnn'nGGC
Cuts at: 2537 2537
Size: 5750
BglII A'GATC_T
Cuts at: 716 3265 5302 716
Size: 2549 2037 1164
Fragments arranged by size:
2549 2037 1164
BlpI GC'TnA_GC
Cuts at: 2862 2862
Size: 5750
Bme1580I G_kGCm'C
Cuts at: 683 1650 2392 3375 3468 4524 683
Size: 967 742 983 93 1056 1909
Fragments arranged by size:
1909 1056 983 967 742 93
BmrI ACTGGGnnnn_n'
Cuts at: 1572 1623 1840 2156 2663 2962 3043 3378 5522
Size: 51 217 316 507 299 81 335 2144
Cuts at: 5522 1572
Size: 1800
Fragments arranged by size:
2144 1800 507 335 316 299 217 81
51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1101 4117 1101
Size: 3016 2734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1096 1180 2254 457
Size: 639 84 1074 3953
Fragments arranged by size:
3953 1074 639 84
Bpu10I CC'TnA_GC
Cuts at: 141 141
Size: 5750
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2926 3805 4297 4595 2926
Size: 879 492 298 4081
Fragments arranged by size:
4081 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5454 5454
Size: 5750
BsaAI yAC'GTr
Cuts at: 744 2441 2687 3103 744
Size: 1697 246 416 3391
Fragments arranged by size:
3391 1697 416 246
BsaBI GATnn'nnATC
Cuts at: 6 1699 3264 5301 6
Size: 1693 1565 2037 455
Fragments arranged by size:
2037 1693 1565 455
BsaHI Gr'CG_yC
Cuts at: 1064 1829 3181 3429 4131 5627 1064
Size: 765 1352 248 702 1496 1187
Fragments arranged by size:
1496 1352 1187 765 702 248
BsaWI w'CCGG_w
Cuts at: 2939 3460 4138 4412 4559 4970 2939
Size: 521 678 274 147 411 3719
Fragments arranged by size:
3719 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1165 1195 1165
Size: 30 5720
BseYI C'CCAG_C
Cuts at: 1013 1088 2049 3175 3933 4510 5006 1013
Size: 75 961 1126 758 577 496 1757
Fragments arranged by size:
1757 1126 961 758 577 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1209 1653 2521 2608 1209
Size: 444 868 87 4351
Fragments arranged by size:
4351 868 444 87
BsiEI CG_ry'CG
Cuts at: 104 1110 3292 3338 4546 104
Size: 1006 2182 46 1208 1308
Fragments arranged by size:
2182 1308 1208 1006 46
BsiHKAI G_wGCw'C
Cuts at: 959 2764 3542 3732 4524 959
Size: 1805 778 190 792 2185
Fragments arranged by size:
2185 1805 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 683 959 1650 2392 2764 3375 3468 3542 3732
Size: 276 691 742 372 611 93 74 190
Cuts at: 3732 3794 4524 683
Size: 62 730 1909
Fragments arranged by size:
1909 742 730 691 611 372 276 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4970 4970
Size: 5750
BspHI T'CATG_A
Cuts at: 4926 4926
Size: 5750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 593 2800 3014 3316 3697 593
Size: 2207 214 302 381 2646
Fragments arranged by size:
2646 2207 381 302 214
BsrBI CCG'CTC
Cuts at: 50 854 4042 4096 5580 5745 50
Size: 804 3188 54 1484 165 55
Fragments arranged by size:
3188 1484 804 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 959 1853 2003 2032 2102 2304 2712 3662
Size: 429 894 150 29 70 202 408 950
Cuts at: 3662 530
Size: 2618
Fragments arranged by size:
2618 950 894 429 408 202 150 70
29
BsrFI r'CCGG_y
Cuts at: 3748 3929 4138 3748
Size: 181 209 5360
Fragments arranged by size:
5360 209 181
BsrGI T'GTAC_A
Cuts at: 1397 1876 2432 1397
Size: 479 556 4715
Fragments arranged by size:
4715 556 479
BssHII G'CGCG_C
Cuts at: 913 1019 913
Size: 106 5644
BssSI C'ACGA_G
Cuts at: 4021 4379 4021
Size: 358 5392
BstAPI GCAn_nnn'nTGC
Cuts at: 824 1326 3127 824
Size: 502 1801 3447
Fragments arranged by size:
3447 1801 502
BstBI TT'CG_AA
Cuts at: 4111 4111
Size: 5750
BstEII G'GTnAC_C
Cuts at: 217 217
Size: 5750
BstXI CCAn_nnnn'nTGG
Cuts at: 576 708 1074 576
Size: 132 366 5252
Fragments arranged by size:
5252 366 132
BstYI r'GATC_y
Cuts at: 1 496 602 716 1376 2775 3242 3265 3600
Size: 495 106 114 660 1399 467 23 335
Cuts at: 3600 3846 4858 4944 4956 5302 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1399 1012 660 495 467 449 346 335
246 114 106 86 23 12
BstZ17I GTA'TAC
Cuts at: 2258 2258
Size: 5750
Bsu36I CC'TnA_GG
Cuts at: 1805 1805
Size: 5750
BtgI C'CryG_G
Cuts at: 707 1304 707
Size: 597 5153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 813 3700 3881 4044 813
Size: 2887 181 163 2519
Fragments arranged by size:
2887 2519 181 163
BtsI GCAGTG_nn'
Cuts at: 567 1772 2993 567
Size: 1205 1221 3324
Fragments arranged by size:
3324 1221 1205
DraI TTT'AAA
Cuts at: 2749 2749
Size: 5750
DraIII CAC_nnn'GTG
Cuts at: 2441 2441
Size: 5750
DrdI GACnn_nn'nnGTC
Cuts at: 744 3456 4314 744
Size: 2712 858 2180
Fragments arranged by size:
2712 2180 858
EaeI y'GGCC_r
Cuts at: 1001 1238 3335 3509 3900 3927 5272 1001
Size: 237 2097 174 391 27 1345 1479
Fragments arranged by size:
2097 1479 1345 391 237 174 27
EagI C'GGCC_G
Cuts at: 3335 3335
Size: 5750
EarI CTCTTCn'nnn_
Cuts at: 601 934 3773 3983 601
Size: 333 2839 210 2368
Fragments arranged by size:
2839 2368 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 853 1087 3958 4268 4414 5560 853
Size: 234 2871 310 146 1146 1043
Fragments arranged by size:
2871 1146 1043 310 234 146
EcoICRI GAG'CTC
Cuts at: 957 2762 957
Size: 1805 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 457 1096 1180 2254 3575 4007 4754 5022
Size: 167 639 84 1074 1321 432 747 268
Cuts at: 5022 290
Size: 1018
Fragments arranged by size:
1321 1074 1018 747 639 432 268 167
84
EcoNI CCTnn'n_nnAGG
Cuts at: 1804 1804
Size: 5750
EcoO109I rG'GnC_Cy
Cuts at: 234 2238 2889 234
Size: 2004 651 3095
Fragments arranged by size:
3095 2004 651
EcoRI G'AATT_C
Cuts at: 2754 2754
Size: 5750
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 238 1425 3769 238
Size: 1187 2344 2219
Fragments arranged by size:
2344 2219 1187
FspI TGC'GCA
Cuts at: 817 937 3531 817
Size: 120 2594 3036
Fragments arranged by size:
3036 2594 120
HaeII r_GCGC'y
Cuts at: 635 1067 2138 3184 3432 4454 5433 635
Size: 432 1071 1046 248 1022 979 952
Fragments arranged by size:
1071 1046 1022 979 952 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 1252 1284 1576 1608 1741 1773 2612
Size: 32 1068 32 292 32 133 32 839
Cuts at: 2612 2644 3592 3622 3624 3654 152
Size: 32 948 30 2 30 2248
Fragments arranged by size:
2248 1068 948 839 292 133 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 119 1590 2789 119
Size: 1471 1199 3080
Fragments arranged by size:
3080 1471 1199
HindIII A'AGCT_T
Cuts at: 1357 1357
Size: 5750
KasI G'GCGC_C
Cuts at: 1063 3180 3428 1063
Size: 2117 248 3385
Fragments arranged by size:
3385 2117 248
KpnI G_GTAC'C
Cuts at: 2770 2770
Size: 5750
MluI A'CGCG_T
Cuts at: 5183 5183
Size: 5750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 4421 4605 881
Size: 3540 184 2026
Fragments arranged by size:
3540 2026 184
MscI TGG'CCA
Cuts at: 1003 1240 3511 1003
Size: 237 2271 3242
Fragments arranged by size:
3242 2271 237
MslI CAynn'nnrTG
Cuts at: 112 574 997 1282 2689 3739 3866 112
Size: 462 423 285 1407 1050 127 1996
Fragments arranged by size:
1996 1407 1050 462 423 285 127
MspA1I CmG'CkG
Cuts at: 2861 3175 3535 4548 4793 2861
Size: 314 360 1013 245 3818
Fragments arranged by size:
3818 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3931 3931
Size: 5750
NarI GG'CG_CC
Cuts at: 1064 3181 3429 1064
Size: 2117 248 3385
Fragments arranged by size:
3385 2117 248
NcoI C'CATG_G
Cuts at: 707 1304 707
Size: 597 5153
NgoMIV G'CCGG_C
Cuts at: 3929 3929
Size: 5750
NsiI A_TGCA'T
Cuts at: 423 1885 423
Size: 1462 4288
NspI r_CATG'y
Cuts at: 404 1054 1883 2319 2803 3303 4210 404
Size: 650 829 436 484 500 907 1944
Fragments arranged by size:
1944 907 829 650 500 484 436
PciI A'CATG_T
Cuts at: 400 4206 400
Size: 3806 1944
PfoI T'CCnGG_A
Cuts at: 1160 1379 1606 2231 1160
Size: 219 227 625 4679
Fragments arranged by size:
4679 625 227 219
PmeI GTTT'AAAC
Cuts at: 2749 2749
Size: 5750
PmlI CAC'GTG
Cuts at: 2441 2441
Size: 5750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 4889
Size: 32 5718
PstI C_TGCA'G
Cuts at: 1779 2797 3482 1779
Size: 1018 685 4047
Fragments arranged by size:
4047 1018 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5750
PvuII CAG'CTG
Cuts at: 3175 3535 3175
Size: 360 5390
RsrII CG'GwC_CG
Cuts at: 3945 3945
Size: 5750
SacI G_AGCT'C
Cuts at: 959 2764 959
Size: 1805 3945
SalI G'TCGA_C
Cuts at: 2787 2787
Size: 5750
SapI GCTCTTCn'nnn_
Cuts at: 601 3773 3983 601
Size: 3172 210 2368
Fragments arranged by size:
3172 2368 210
SbfI CC_TGCA'GG
Cuts at: 2797 2797
Size: 5750
ScaI AGT'ACT
Cuts at: 5267 5267
Size: 5750
SfcI C'TryA_G
Cuts at: 33 1775 2493 2793 3478 4471 4662 33
Size: 1742 718 300 685 993 191 1121
Fragments arranged by size:
1742 1121 993 718 685 300 191
SfoI GGC'GCC
Cuts at: 1065 3182 3430 1065
Size: 2117 248 3385
Fragments arranged by size:
3385 2117 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5750
SmaI CCC'GGG
Cuts at: 2772 2772
Size: 5750
SmlI C'TyrA_G
Cuts at: 2905 3820 4312 4574 2905
Size: 915 492 262 4081
Fragments arranged by size:
4081 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5750
SphI G_CATG'C
Cuts at: 2803 3303 2803
Size: 500 5250
StuI AGG'CCT
Cuts at: 793 793
Size: 5750
StyI C'CwwG_G
Cuts at: 707 1304 1922 2570 2884 3238 707
Size: 597 618 648 314 354 3219
Fragments arranged by size:
3219 648 618 597 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 311 370 987 2583 3333 3466 3925 4134 311
Size: 59 617 1596 750 133 459 209 1927
Fragments arranged by size:
1927 1596 750 617 459 209 133 59
TatI w'GTAC_w
Cuts at: 1397 1876 2432 5265 1397
Size: 479 556 2833 1882
Fragments arranged by size:
2833 1882 556 479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1326 1358 5267 5299 1326
Size: 32 3909 32 1777
Fragments arranged by size:
3909 1777 32 32
Tth111I GACn'n_nGTC
Cuts at: 3547 3547
Size: 5750
XbaI T'CTAG_A
Cuts at: 2781 2781
Size: 5750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1528 1860 2739 3245 1528
Size: 332 879 506 4033
Fragments arranged by size:
4033 879 506 332
XmaI C'CCGG_G
Cuts at: 2770 2770
Size: 5750
XmnI GAAnn'nnTTC
Cuts at: 1539 1539
Size: 5750
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BmgBI BmtI BseRI BsiWI BsmI
BsmBI ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI