Restriction map for
pF1KB8486
(Circular) (Six-base) MAPSORT of: pF1KB8486.seq Check: 7115 from: 1 to: 5678
pF1KB8486 5678 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1303 2440 1303
Size: 1137 4541
AccI GT'mk_AC
Cuts at: 2716 2716
Size: 5678
Acc65I G'GTAC_C
Cuts at: 2694 2694
Size: 5678
AclI AA'CG_TT
Cuts at: 5518 5518
Size: 5678
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 528 691 1680 3503 3935 4682 4950 499
Size: 29 163 989 1823 432 747 268 1227
Fragments arranged by size:
1823 1227 989 747 432 268 163 29
AflIII A'CryG_T
Cuts at: 3030 4134 5111 3030
Size: 1104 977 3597
Fragments arranged by size:
3597 1104 977
AgeI A'CCGG_T
Cuts at: 4066 4066
Size: 5678
AhdI GACnn_n'nnGTC
Cuts at: 903 903
Size: 5678
AleI CACnn'nnGTG
Cuts at: 691 691
Size: 5678
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 418
Size: 32 5646
AlwNI CAG_nnn'CTG
Cuts at: 600 2983 4550 600
Size: 2383 1567 1728
Fragments arranged by size:
2383 1728 1567
ApaI G_GGCC'C
Cuts at: 126 145 514 849 126
Size: 19 369 335 4955
Fragments arranged by size:
4955 369 335 19
ApaLI G'TGCA_C
Cuts at: 4448 4448
Size: 5678
ApoI r'AATT_y
Cuts at: 405 1255 2682 5031 5287 405
Size: 850 1427 2349 256 796
Fragments arranged by size:
2349 1427 850 796 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5678
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5678
AvaI C'yCGr_G
Cuts at: 261 1467 2698 261
Size: 1206 1231 3241
Fragments arranged by size:
3241 1231 1206
AvrII C'CTAG_G
Cuts at: 1111 1111
Size: 5678
BamHI G'GATC_C
Cuts at: 2703 2703
Size: 5678
BanI G'GyrC_C
Cuts at: 991 1221 1291 2694 3108 3356 3391 991
Size: 230 70 1403 414 248 35 3278
Fragments arranged by size:
3278 1403 414 248 230 70 35
BanII G_rGCy'C
Cuts at: 126 145 514 849 958 968 1277 1559 1841
Size: 19 369 335 109 10 309 282 282
Cuts at: 1841 2692 3722 126
Size: 851 1030 2082
Fragments arranged by size:
2082 1030 851 369 335 309 282 282
109 19 10
BbeI G_GCGC'C
Cuts at: 3112 3360 3112
Size: 248 5430
BbsI GAAGACnn'nnnn_
Cuts at: 366 2313 366
Size: 1947 3731
BbvCI CC'TCA_GC
Cuts at: 2488 2488
Size: 5678
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 543 577 543
Size: 34 5644
BciVI GTATCCnnnnn_n'
Cuts at: 1510 3576 4337 5678 1510
Size: 2066 761 1341 1510
Fragments arranged by size:
2066 1510 1341 761
BclI T'GATC_A
Cuts at: 3198 3198
Size: 5678
BglI GCCn_nnn'nGGC
Cuts at: 2335 2335
Size: 5678
BglII A'GATC_T
Cuts at: 3193 5230 3193
Size: 2037 3641
BlpI GC'TnA_GC
Cuts at: 874 2790 874
Size: 1916 3762
Bme1580I G_kGCm'C
Cuts at: 126 145 514 849 1226 1294 1963 3303 3396
Size: 19 369 335 377 68 669 1340 93
Cuts at: 3396 4452 126
Size: 1056 1352
Fragments arranged by size:
1352 1340 1056 669 377 369 335 93
68 19
BmgBI CAC'GTC
Cuts at: 357 357
Size: 5678
BmrI ACTGGGnnnn_n'
Cuts at: 1344 2361 2675 2890 2971 3306 5450 1344
Size: 1017 314 215 81 335 2144 1572
Fragments arranged by size:
2144 1572 1017 335 314 215 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1961 2295 4045 1961
Size: 334 1750 3594
Fragments arranged by size:
3594 1750 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 1437 2272 2278 1291
Size: 146 835 6 4691
Fragments arranged by size:
4691 835 146 6
Bpu10I CC'TnA_GC
Cuts at: 952 2488 952
Size: 1536 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1866 2173 2854 3733 4225 4523 151
Size: 1715 307 681 879 492 298 1306
Fragments arranged by size:
1715 1306 879 681 492 307 298
BsaI GGTCTCn'nnnn_
Cuts at: 2067 5382 2067
Size: 3315 2363
BsaAI yAC'GTr
Cuts at: 1141 1311 1481 1592 3031 1141
Size: 170 170 111 1439 3788
Fragments arranged by size:
3788 1439 170 170 111
BsaBI GATnn'nnATC
Cuts at: 6 3192 5229 6
Size: 3186 2037 455
Fragments arranged by size:
3186 2037 455
BsaHI Gr'CG_yC
Cuts at: 860 1574 2309 3109 3357 4059 5555 860
Size: 714 735 800 248 702 1496 983
Fragments arranged by size:
1496 983 800 735 714 702 248
BsaWI w'CCGG_w
Cuts at: 1502 2867 3388 4066 4340 4487 4898 1502
Size: 1365 521 678 274 147 411 2282
Fragments arranged by size:
2282 1365 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 412 418 442 448 984 988 1014 1018 1762
Size: 6 24 6 536 4 26 4 744
Cuts at: 1762 1792 412
Size: 30 4298
Fragments arranged by size:
4298 744 536 30 26 24 6 6
4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 622 646 1017 292
Size: 330 24 371 4953
Fragments arranged by size:
4953 371 330 24
BseYI C'CCAG_C
Cuts at: 269 315 1526 2354 2582 3103 3861 4438 4934
Size: 46 1211 828 228 521 758 577 496
Cuts at: 4934 269
Size: 1013
Fragments arranged by size:
1211 1013 828 758 577 521 496 228
46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1000 1188 1521 1695 2194 745
Size: 255 188 333 174 499 4229
Fragments arranged by size:
4229 499 333 255 188 174
BsiEI CG_ry'CG
Cuts at: 104 120 3220 3266 4474 104
Size: 16 3100 46 1208 1308
Fragments arranged by size:
3100 1308 1208 46 16
BsiHKAI G_wGCw'C
Cuts at: 1559 2692 3470 3660 4452 1559
Size: 1133 778 190 792 2785
Fragments arranged by size:
2785 1133 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2582 2582
Size: 5678
Bsp1286I G_dGCh'C
Cuts at: 126 145 514 849 958 968 1226 1277 1294
Size: 19 369 335 109 10 258 51 17
Cuts at: 1294 1559 1841 1963 2692 3303 3396 3470 3660
Size: 265 282 122 729 611 93 74 190
Cuts at: 3660 3722 4452 126
Size: 62 730 1352
Fragments arranged by size:
1352 730 729 611 369 335 282 265
258 190 122 109 93 74 62 51
19 17 10
BspEI T'CCGG_A
Cuts at: 1502 4898 1502
Size: 3396 2282
BspHI T'CATG_A
Cuts at: 4854 4854
Size: 5678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1274 1303 1983 2440 2728 2942 3244 3625 1274
Size: 29 680 457 288 214 302 381 3327
Fragments arranged by size:
3327 680 457 381 302 288 214 29
BsrBI CCG'CTC
Cuts at: 50 826 1071 3970 4024 5508 5673 50
Size: 776 245 2899 54 1484 165 55
Fragments arranged by size:
2899 1484 776 245 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1746 3590 1746
Size: 1844 3834
BsrFI r'CCGG_y
Cuts at: 1420 1576 2265 2329 2569 3676 3857 4066 1420
Size: 156 689 64 240 1107 181 209 3032
Fragments arranged by size:
3032 1107 689 240 209 181 156 64
BsrGI T'GTAC_A
Cuts at: 488 941 2621 488
Size: 453 1680 3545
Fragments arranged by size:
3545 1680 453
BssHII G'CGCG_C
Cuts at: 661 2384 661
Size: 1723 3955
BssSI C'ACGA_G
Cuts at: 184 1935 1946 3949 4307 184
Size: 1751 11 2003 358 1555
Fragments arranged by size:
2003 1751 1555 358 11
BstAPI GCAn_nnn'nTGC
Cuts at: 2474 3055 2474
Size: 581 5097
BstBI TT'CG_AA
Cuts at: 4039 4039
Size: 5678
BstEII G'GTnAC_C
Cuts at: 684 684
Size: 5678
BstXI CCAn_nnnn'nTGG
Cuts at: 2058 2058
Size: 5678
BstYI r'GATC_y
Cuts at: 1 2408 2558 2703 3170 3193 3528 3774 4786
Size: 2407 150 145 467 23 335 246 1012
Cuts at: 4786 4872 4884 5230 1
Size: 86 12 346 449
Fragments arranged by size:
2407 1012 467 449 346 335 246 150
145 86 23 12
BtgI C'CryG_G
Cuts at: 107 655 707 768 959 2282 107
Size: 548 52 61 191 1323 3503
Fragments arranged by size:
3503 1323 548 191 61 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 546 1565 2226 3628 3809 3972 546
Size: 1019 661 1402 181 163 2252
Fragments arranged by size:
2252 1402 1019 661 181 163
BtsI GCAGTG_nn'
Cuts at: 1741 2073 2125 2921 1741
Size: 332 52 796 4498
Fragments arranged by size:
4498 796 332 52
ClaI AT'CG_AT
Cuts at: 2084 2084
Size: 5678
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 426 461 426
Size: 35 5643
DraI TTT'AAA
Cuts at: 2677 2677
Size: 5678
DraIII CAC_nnn'GTG
Cuts at: 603 2120 603
Size: 1517 4161
DrdI GACnn_nn'nnGTC
Cuts at: 361 3384 4242 361
Size: 3023 858 1797
Fragments arranged by size:
3023 1797 858
EaeI y'GGCC_r
Cuts at: 455 626 1578 3263 3437 3828 3855 5200 455
Size: 171 952 1685 174 391 27 1345 933
Fragments arranged by size:
1685 1345 952 933 391 174 171 27
EagI C'GGCC_G
Cuts at: 3263 3263
Size: 5678
EarI CTCTTCn'nnn_
Cuts at: 1624 3701 3911 1624
Size: 2077 210 3391
Fragments arranged by size:
3391 2077 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 149 2563 2571 3886 4196 4342 5488 149
Size: 2414 8 1315 310 146 1146 339
Fragments arranged by size:
2414 1315 1146 339 310 146 8
EcoICRI GAG'CTC
Cuts at: 1557 2690 1557
Size: 1133 4545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 528 691 1291 1437 1680 2272 2278 3503
Size: 29 163 600 146 243 592 6 1225
Cuts at: 3503 3935 4682 4950 499
Size: 432 747 268 1227
Fragments arranged by size:
1227 1225 747 600 592 432 268 243
163 146 29 6
EcoNI CCTnn'n_nnAGG
Cuts at: 604 2398 604
Size: 1794 3884
EcoO109I rG'GnC_Cy
Cuts at: 141 511 731 846 1346 2527 2817 141
Size: 370 220 115 500 1181 290 3002
Fragments arranged by size:
3002 1181 500 370 290 220 115
EcoRI G'AATT_C
Cuts at: 2682 2682
Size: 5678
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1266 1635 3697 1266
Size: 369 2062 3247
Fragments arranged by size:
3247 2062 369
FspI TGC'GCA
Cuts at: 3459 3459
Size: 5678
HaeII r_GCGC'y
Cuts at: 270 2165 3112 3360 4382 5361 270
Size: 1895 947 248 1022 979 587
Fragments arranged by size:
1895 1022 979 947 587 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 850 882 889 921 1762 1794 3520 3550 3552
Size: 32 7 32 841 32 1726 30 2
Cuts at: 3552 3582 850
Size: 30 2946
Fragments arranged by size:
2946 1726 841 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 231 2717 231
Size: 2486 3192
KasI G'GCGC_C
Cuts at: 3108 3356 3108
Size: 248 5430
KpnI G_GTAC'C
Cuts at: 2698 2698
Size: 5678
MluI A'CGCG_T
Cuts at: 5111 5111
Size: 5678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 455 1793 4349 4533 455
Size: 1338 2556 184 1600
Fragments arranged by size:
2556 1600 1338 184
MscI TGG'CCA
Cuts at: 457 3439 457
Size: 2982 2696
MslI CAynn'nnrTG
Cuts at: 691 1219 1550 3667 3794 691
Size: 528 331 2117 127 2575
Fragments arranged by size:
2575 2117 528 331 127
MspA1I CmG'CkG
Cuts at: 494 657 746 770 836 1107 1124 1826 2492
Size: 163 89 24 66 271 17 702 666
Cuts at: 2492 2789 3103 3463 4476 4721 494
Size: 297 314 360 1013 245 1451
Fragments arranged by size:
1451 1013 702 666 360 314 297 271
245 163 89 66 24 17
NaeI GCC'GGC
Cuts at: 1578 2331 3859 1578
Size: 753 1528 3397
Fragments arranged by size:
3397 1528 753
NarI GG'CG_CC
Cuts at: 3109 3357 3109
Size: 248 5430
NcoI C'CATG_G
Cuts at: 107 959 2282 107
Size: 852 1323 3503
Fragments arranged by size:
3503 1323 852
NgoMIV G'CCGG_C
Cuts at: 1576 2329 3857 1576
Size: 753 1528 3397
Fragments arranged by size:
3397 1528 753
NspI r_CATG'y
Cuts at: 1240 2008 2731 3231 4138 1240
Size: 768 723 500 907 2780
Fragments arranged by size:
2780 907 768 723 500
PasI CC'CwG_GG
Cuts at: 1149 2167 1149
Size: 1018 4660
PciI A'CATG_T
Cuts at: 4134 4134
Size: 5678
PflMI CCAn_nnn'nTGG
Cuts at: 975 2120 975
Size: 1145 4533
PmeI GTTT'AAAC
Cuts at: 2677 2677
Size: 5678
PmlI CAC'GTG
Cuts at: 1481 1592 1481
Size: 111 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 418 450 4817 4849 418
Size: 32 4367 32 1247
Fragments arranged by size:
4367 1247 32 32
PpuMI rG'GwC_Cy
Cuts at: 731 1346 2527 731
Size: 615 1181 3882
Fragments arranged by size:
3882 1181 615
PshAI GACnn'nnGTC
Cuts at: 2532 2532
Size: 5678
PspOMI G'GGCC_C
Cuts at: 122 141 510 845 122
Size: 19 369 335 4955
Fragments arranged by size:
4955 369 335 19
PstI C_TGCA'G
Cuts at: 751 1567 2725 3410 751
Size: 816 1158 685 3019
Fragments arranged by size:
3019 1158 816 685
PvuI CG_AT'CG
Cuts at: 104 120 104
Size: 16 5662
PvuII CAG'CTG
Cuts at: 746 1107 1124 3103 3463 746
Size: 361 17 1979 360 2961
Fragments arranged by size:
2961 1979 361 360 17
RsrII CG'GwC_CG
Cuts at: 3873 3873
Size: 5678
SacI G_AGCT'C
Cuts at: 1559 2692 1559
Size: 1133 4545
SacII CC_GC'GG
Cuts at: 658 771 658
Size: 113 5565
SalI G'TCGA_C
Cuts at: 2715 2715
Size: 5678
SanDI GG'GwC_CC
Cuts at: 2527 2527
Size: 5678
SapI GCTCTTCn'nnn_
Cuts at: 3701 3911 3701
Size: 210 5468
SbfI CC_TGCA'GG
Cuts at: 2725 2725
Size: 5678
ScaI AGT'ACT
Cuts at: 5195 5195
Size: 5678
SexAI A'CCwGG_T
Cuts at: 599 929 599
Size: 330 5348
SfcI C'TryA_G
Cuts at: 33 747 1563 1884 2721 3406 4399 4590 33
Size: 714 816 321 837 685 993 191 1121
Fragments arranged by size:
1121 993 837 816 714 685 321 191
SfoI GGC'GCC
Cuts at: 3110 3358 3110
Size: 248 5430
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5678
SmaI CCC'GGG
Cuts at: 263 1469 2700 263
Size: 1206 1231 3241
Fragments arranged by size:
3241 1231 1206
SmlI C'TyrA_G
Cuts at: 166 1845 2188 2833 3748 4240 4502 166
Size: 1679 343 645 915 492 262 1342
Fragments arranged by size:
1679 1342 915 645 492 343 262
SnaBI TAC'GTA
Cuts at: 1141 1141
Size: 5678
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5678
SphI G_CATG'C
Cuts at: 2008 2731 3231 2008
Size: 723 500 4455
Fragments arranged by size:
4455 723 500
StuI AGG'CCT
Cuts at: 1777 1777
Size: 5678
StyI C'CwwG_G
Cuts at: 107 959 1111 1301 1787 2282 2812 3166 107
Size: 852 152 190 486 495 530 354 2619
Fragments arranged by size:
2619 852 530 495 486 354 190 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 1206 2380 3261 3394 3853 4062 261
Size: 945 1174 881 133 459 209 1877
Fragments arranged by size:
1877 1174 945 881 459 209 133
TatI w'GTAC_w
Cuts at: 170 488 723 941 2273 2621 5193 170
Size: 318 235 218 1332 348 2572 655
Fragments arranged by size:
2572 1332 655 348 318 235 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 984 1016 5195 5227 984
Size: 32 4179 32 1435
Fragments arranged by size:
4179 1435 32 32
Tth111I GACn'n_nGTC
Cuts at: 3475 3475
Size: 5678
XbaI T'CTAG_A
Cuts at: 2709 2709
Size: 5678
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1308 1738 3173 1308
Size: 430 1435 3813
Fragments arranged by size:
3813 1435 430
XmaI C'CCGG_G
Cuts at: 261 1467 2698 261
Size: 1206 1231 3241
Fragments arranged by size:
3241 1231 1206
XmnI GAAnn'nnTTC
Cuts at: 151 373 1603 151
Size: 222 1230 4226
Fragments arranged by size:
4226 1230 222
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AscI BaeI BfrBI BmtI
BsiWI BsmI BstZ17I Bsu36I EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PsiI PspXI PsrI SfiI SgrAI SrfI SspI
SwaI XhoI ZraI