Restriction map for
pF1KB8474
(Circular) (Six-base) MAPSORT of: pF1KB8474.seq Check: 3386 from: 1 to: 5636
pF1KB8474 5636 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1305 1473 2674 1305
Size: 168 1201 4267
Fragments arranged by size:
4267 1201 168
Acc65I G'GTAC_C
Cuts at: 2652 2652
Size: 5636
AclI AA'CG_TT
Cuts at: 5476 5476
Size: 5636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 1801 1876 1894 2100 3461 3893 4640 4908
Size: 1323 75 18 206 1361 432 747 268
Cuts at: 4908 478
Size: 1206
Fragments arranged by size:
1361 1323 1206 747 432 268 206 75
18
AflIII A'CryG_T
Cuts at: 2988 4092 5069 2988
Size: 1104 977 3555
Fragments arranged by size:
3555 1104 977
AgeI A'CCGG_T
Cuts at: 4024 4024
Size: 5636
AhdI GACnn_n'nnGTC
Cuts at: 238 238
Size: 5636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1787 1787
Size: 5636
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 244
Size: 32 5604
AlwNI CAG_nnn'CTG
Cuts at: 159 456 948 2250 2941 4508 159
Size: 297 492 1302 691 1567 1287
Fragments arranged by size:
1567 1302 1287 691 492 297
ApaI G_GGCC'C
Cuts at: 500 643 1044 500
Size: 143 401 5092
Fragments arranged by size:
5092 401 143
ApaLI G'TGCA_C
Cuts at: 4406 4406
Size: 5636
ApoI r'AATT_y
Cuts at: 2640 4989 5245 2640
Size: 2349 256 3031
Fragments arranged by size:
3031 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5636
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
AvaI C'yCGr_G
Cuts at: 1822 2656 1822
Size: 834 4802
BamHI G'GATC_C
Cuts at: 627 1910 2661 627
Size: 1283 751 3602
Fragments arranged by size:
3602 1283 751
BanI G'GyrC_C
Cuts at: 2652 3066 3314 3349 2652
Size: 414 248 35 4939
Fragments arranged by size:
4939 414 248 35
BanII G_rGCy'C
Cuts at: 500 643 754 1044 1526 1568 2152 2240 2650
Size: 143 111 290 482 42 584 88 410
Cuts at: 2650 3680 500
Size: 1030 2456
Fragments arranged by size:
2456 1030 584 482 410 290 143 111
88 42
BbeI G_GCGC'C
Cuts at: 3070 3318 3070
Size: 248 5388
BbsI GAAGACnn'nnnn_
Cuts at: 220 1547 1864 220
Size: 1327 317 3992
Fragments arranged by size:
3992 1327 317
BbvCI CC'TCA_GC
Cuts at: 520 520
Size: 5636
BciVI GTATCCnnnnn_n'
Cuts at: 1068 3534 4295 5636 1068
Size: 2466 761 1341 1068
Fragments arranged by size:
2466 1341 1068 761
BclI T'GATC_A
Cuts at: 915 3156 915
Size: 2241 3395
BglI GCCn_nnn'nGGC
Cuts at: 931 2036 2202 931
Size: 1105 166 4365
Fragments arranged by size:
4365 1105 166
BglII A'GATC_T
Cuts at: 2129 3151 5188 2129
Size: 1022 2037 2577
Fragments arranged by size:
2577 2037 1022
BlpI GC'TnA_GC
Cuts at: 152 794 2234 2575 2748 152
Size: 642 1440 341 173 3040
Fragments arranged by size:
3040 1440 642 341 173
Bme1580I G_kGCm'C
Cuts at: 500 643 1044 1509 3261 3354 4410 500
Size: 143 401 465 1752 93 1056 1726
Fragments arranged by size:
1752 1726 1056 465 401 143 93
BmrI ACTGGGnnnn_n'
Cuts at: 921 1208 2848 2929 3264 5408 921
Size: 287 1640 81 335 2144 1149
Fragments arranged by size:
2144 1640 1149 335 287 81
BmtI G_CTAG'C
Cuts at: 1417 1417
Size: 5636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2085 4003 2085
Size: 1918 3718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1162 1264 1603 1630 1936 2302 2524 457
Size: 705 102 339 27 306 366 222 3569
Fragments arranged by size:
3569 705 366 339 306 222 102 27
Bpu10I CC'TnA_GC
Cuts at: 302 520 1646 2034 302
Size: 218 1126 388 3904
Fragments arranged by size:
3904 1126 388 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1140 1732 1924 1933 2062 2171 2812 3691
Size: 158 592 192 9 129 109 641 879
Cuts at: 3691 4183 4481 982
Size: 492 298 2137
Fragments arranged by size:
2137 879 641 592 492 298 192 158
129 109 9
BsaI GGTCTCn'nnnn_
Cuts at: 2353 2435 5340 2353
Size: 82 2905 2649
Fragments arranged by size:
2905 2649 82
BsaAI yAC'GTr
Cuts at: 2989 2989
Size: 5636
BsaBI GATnn'nnATC
Cuts at: 6 3150 5187 6
Size: 3144 2037 455
Fragments arranged by size:
3144 2037 455
BsaHI Gr'CG_yC
Cuts at: 3067 3315 4017 5513 3067
Size: 248 702 1496 3190
Fragments arranged by size:
3190 1496 702 248
BsaWI w'CCGG_w
Cuts at: 167 2825 3346 4024 4298 4445 4856 167
Size: 2658 521 678 274 147 411 947
Fragments arranged by size:
2658 947 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 467 497 847 877 1255 1285 467
Size: 30 350 30 378 30 4818
Fragments arranged by size:
4818 378 350 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 145 379 724 1573 1576 1600 1807 1810
Size: 3 234 345 849 3 24 207 3
Cuts at: 1810 1852 2059 2100 2299 2521 142
Size: 42 207 41 199 222 3257
Fragments arranged by size:
3257 849 345 234 222 207 207 199
42 41 24 3 3 3
BseYI C'CCAG_C
Cuts at: 493 1274 1442 1458 1494 2192 2424 3061 3819
Size: 781 168 16 36 698 232 637 758
Cuts at: 3819 4396 4892 493
Size: 577 496 1237
Fragments arranged by size:
1237 781 758 698 637 577 496 232
168 36 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1091 337
Size: 754 4882
BsiEI CG_ry'CG
Cuts at: 104 3178 3224 4432 104
Size: 3074 46 1208 1308
Fragments arranged by size:
3074 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 754 1526 1568 2152 2240 2650 3428 3618 4410
Size: 772 42 584 88 410 778 190 792
Cuts at: 4410 754
Size: 1980
Fragments arranged by size:
1980 792 778 772 584 410 190 88
42
Bsp1286I G_dGCh'C
Cuts at: 500 643 754 1044 1509 1526 1568 2152 2240
Size: 143 111 290 465 17 42 584 88
Cuts at: 2240 2650 3261 3354 3428 3618 3680 4410 500
Size: 410 611 93 74 190 62 730 1726
Fragments arranged by size:
1726 730 611 584 465 410 290 190
143 111 93 88 74 62 42 17
BspEI T'CCGG_A
Cuts at: 167 4856 167
Size: 4689 947
BspHI T'CATG_A
Cuts at: 4812 4812
Size: 5636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2686 2900 3202 3583 2686
Size: 214 302 381 4739
Fragments arranged by size:
4739 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1842 1971 2033 3928 3982 5466 5631 50
Size: 1792 129 62 1895 54 1484 165 55
Fragments arranged by size:
1895 1792 1484 165 129 62 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 3548 1424
Size: 2124 3512
BsrFI r'CCGG_y
Cuts at: 3634 3815 4024 3634
Size: 181 209 5246
Fragments arranged by size:
5246 209 181
BssSI C'ACGA_G
Cuts at: 502 3907 4265 502
Size: 3405 358 1873
Fragments arranged by size:
3405 1873 358
BstAPI GCAn_nnn'nTGC
Cuts at: 416 484 2499 2585 3013 416
Size: 68 2015 86 428 3039
Fragments arranged by size:
3039 2015 428 86 68
BstBI TT'CG_AA
Cuts at: 3997 3997
Size: 5636
BstXI CCAn_nnnn'nTGG
Cuts at: 657 672 1203 657
Size: 15 531 5090
Fragments arranged by size:
5090 531 15
BstYI r'GATC_y
Cuts at: 1 627 1148 1577 1910 2129 2661 3128 3151
Size: 626 521 429 333 219 532 467 23
Cuts at: 3151 3486 3732 4744 4830 4842 5188 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 626 532 521 467 449 429 346
335 333 246 219 86 23 12
BtgI C'CryG_G
Cuts at: 107 1569 1658 2321 107
Size: 1462 89 663 3422
Fragments arranged by size:
3422 1462 663 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3586 3767 3930 3586
Size: 181 163 5292
Fragments arranged by size:
5292 181 163
BtsI GCAGTG_nn'
Cuts at: 1759 1935 2319 2879 1759
Size: 176 384 560 4516
Fragments arranged by size:
4516 560 384 176
ClaI AT'CG_AT
Cuts at: 2384 2384
Size: 5636
DraI TTT'AAA
Cuts at: 2635 2635
Size: 5636
DrdI GACnn_nn'nnGTC
Cuts at: 3342 4200 3342
Size: 858 4778
EaeI y'GGCC_r
Cuts at: 398 2203 3221 3395 3786 3813 5158 398
Size: 1805 1018 174 391 27 1345 876
Fragments arranged by size:
1805 1345 1018 876 391 174 27
EagI C'GGCC_G
Cuts at: 3221 3221
Size: 5636
EarI CTCTTCn'nnn_
Cuts at: 709 1372 1448 2338 3659 3869 709
Size: 663 76 890 1321 210 2476
Fragments arranged by size:
2476 1321 890 663 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3844 4154 4300 5446 3844
Size: 310 146 1146 4034
Fragments arranged by size:
4034 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 1524 1566 2150 2238 2648 752
Size: 772 42 584 88 410 3740
Fragments arranged by size:
3740 772 584 410 88 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 478 1162 1264 1603 1630 1801 1876 1894
Size: 21 684 102 339 27 171 75 18
Cuts at: 1894 1936 2100 2302 2524 3461 3893 4640 4908
Size: 42 164 202 222 937 432 747 268
Cuts at: 4908 457
Size: 1185
Fragments arranged by size:
1185 937 747 684 432 339 268 222
202 171 164 102 75 42 27 21
18
EcoNI CCTnn'n_nnAGG
Cuts at: 1876 1876
Size: 5636
EcoO109I rG'GnC_Cy
Cuts at: 497 639 1041 1242 1944 2168 2331 2593 2775
Size: 142 402 201 702 224 163 262 182
Cuts at: 2775 497
Size: 3358
Fragments arranged by size:
3358 702 402 262 224 201 182 163
142
EcoRI G'AATT_C
Cuts at: 2640 2640
Size: 5636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5636
FspI TGC'GCA
Cuts at: 3417 3417
Size: 5636
HaeII r_GCGC'y
Cuts at: 1017 1692 2351 3070 3318 4340 5319 1017
Size: 675 659 719 248 1022 979 1334
Fragments arranged by size:
1334 1022 979 719 675 659 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 224 256 1042 1074 1255 1287 3478
Size: 32 57 32 786 32 181 32 2191
Cuts at: 3478 3508 3510 3540 135
Size: 30 2 30 2231
Fragments arranged by size:
2231 2191 786 181 57 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1306 1352 2675 1306
Size: 46 1323 4267
Fragments arranged by size:
4267 1323 46
KasI G'GCGC_C
Cuts at: 3066 3314 3066
Size: 248 5388
KpnI G_GTAC'C
Cuts at: 2656 2656
Size: 5636
MluI A'CGCG_T
Cuts at: 5069 5069
Size: 5636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4307 4491 4307
Size: 184 5452
MscI TGG'CCA
Cuts at: 3397 3397
Size: 5636
MslI CAynn'nnrTG
Cuts at: 394 3625 3752 394
Size: 3231 127 2278
Fragments arranged by size:
3231 2278 127
MspA1I CmG'CkG
Cuts at: 141 435 607 735 1002 1210 1959 2247 2420
Size: 294 172 128 267 208 749 288 173
Cuts at: 2420 2747 3061 3421 4434 4679 141
Size: 327 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 749 360 327 314 294 288
267 245 208 173 172 128
NaeI GCC'GGC
Cuts at: 3817 3817
Size: 5636
NarI GG'CG_CC
Cuts at: 3067 3315 3067
Size: 248 5388
NcoI C'CATG_G
Cuts at: 107 1658 2321 107
Size: 1551 663 3422
Fragments arranged by size:
3422 1551 663
NgoMIV G'CCGG_C
Cuts at: 3815 3815
Size: 5636
NheI G'CTAG_C
Cuts at: 1413 1413
Size: 5636
NspI r_CATG'y
Cuts at: 1027 2502 2689 3189 4096 1027
Size: 1475 187 500 907 2567
Fragments arranged by size:
2567 1475 907 500 187
PasI CC'CwG_GG
Cuts at: 634 634
Size: 5636
PciI A'CATG_T
Cuts at: 4092 4092
Size: 5636
PflMI CCAn_nnn'nTGG
Cuts at: 1676 1676
Size: 5636
PfoI T'CCnGG_A
Cuts at: 158 1184 1280 1580 1913 158
Size: 1026 96 300 333 3881
Fragments arranged by size:
3881 1026 333 300 96
PmeI GTTT'AAAC
Cuts at: 2635 2635
Size: 5636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4775 4807 4775
Size: 32 5604
PpuMI rG'GwC_Cy
Cuts at: 1242 1944 2168 2331 1242
Size: 702 224 163 4547
Fragments arranged by size:
4547 702 224 163
PshAI GACnn'nnGTC
Cuts at: 2365 2365
Size: 5636
PspOMI G'GGCC_C
Cuts at: 496 639 1040 496
Size: 143 401 5092
Fragments arranged by size:
5092 401 143
PspXI vC'TCGA_Gb
Cuts at: 1822 1822
Size: 5636
PstI C_TGCA'G
Cuts at: 344 421 474 572 782 845 953 1718 1994
Size: 77 53 98 210 63 108 765 276
Cuts at: 1994 2006 2495 2683 3368 344
Size: 12 489 188 685 2612
Fragments arranged by size:
2612 765 685 489 276 210 188 108
98 77 63 53 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5636
PvuII CAG'CTG
Cuts at: 435 607 735 1210 1959 3061 3421 435
Size: 172 128 475 749 1102 360 2650
Fragments arranged by size:
2650 1102 749 475 360 172 128
RsrII CG'GwC_CG
Cuts at: 3831 3831
Size: 5636
SacI G_AGCT'C
Cuts at: 754 1526 1568 2152 2240 2650 754
Size: 772 42 584 88 410 3740
Fragments arranged by size:
3740 772 584 410 88 42
SalI G'TCGA_C
Cuts at: 1304 2673 1304
Size: 1369 4267
SapI GCTCTTCn'nnn_
Cuts at: 1448 2338 3659 3869 1448
Size: 890 1321 210 3215
Fragments arranged by size:
3215 1321 890 210
SbfI CC_TGCA'GG
Cuts at: 572 2683 572
Size: 2111 3525
ScaI AGT'ACT
Cuts at: 5153 5153
Size: 5636
SfcI C'TryA_G
Cuts at: 33 340 417 470 526 568 778 841 949
Size: 307 77 53 56 42 210 63 108
Cuts at: 949 1714 1801 1990 2002 2491 2679 3364 4357
Size: 765 87 189 12 489 188 685 993
Cuts at: 4357 4548 33
Size: 191 1121
Fragments arranged by size:
1121 993 765 685 489 307 210 191
189 188 108 87 77 63 56 53
42 12
SfoI GGC'GCC
Cuts at: 3068 3316 3068
Size: 248 5388
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
SmaI CCC'GGG
Cuts at: 2658 2658
Size: 5636
SmlI C'TyrA_G
Cuts at: 961 1119 1747 1822 1939 1948 2041 2186 2791
Size: 158 628 75 117 9 93 145 605
Cuts at: 2791 3706 4198 4460 961
Size: 915 492 262 2137
Fragments arranged by size:
2137 915 628 605 492 262 158 145
117 93 75 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5636
SphI G_CATG'C
Cuts at: 2689 3189 2689
Size: 500 5136
StuI AGG'CCT
Cuts at: 2039 2039
Size: 5636
StyI C'CwwG_G
Cuts at: 107 1658 1670 2321 2770 3124 107
Size: 1551 12 651 449 354 2619
Fragments arranged by size:
2619 1551 651 449 354 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3219 3352 3811 4020 3219
Size: 133 459 209 4835
Fragments arranged by size:
4835 459 209 133
TatI w'GTAC_w
Cuts at: 5151 5151
Size: 5636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5153 5185 5153
Size: 32 5604
Tth111I GACn'n_nGTC
Cuts at: 3433 3433
Size: 5636
XbaI T'CTAG_A
Cuts at: 2667 2667
Size: 5636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 978 3131 978
Size: 2153 3483
XhoI C'TCGA_G
Cuts at: 1822 1822
Size: 5636
XmaI C'CCGG_G
Cuts at: 2656 2656
Size: 5636
XmnI GAAnn'nnTTC
Cuts at: 1319 1637 1319
Size: 318 5318
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BaeI
BcgI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI