Restriction map for
pF1KB8468
(Circular) (Six-base) MAPSORT of: pF1KB8468.seq Check: 6397 from: 1 to: 4004
pF1KB8468 4004 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 171 1042 171
Size: 871 3133
Acc65I G'GTAC_C
Cuts at: 145 1020 145
Size: 875 3129
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 388 732 1829 2261 3008 3276 289
Size: 99 344 1097 432 747 268 1017
Fragments arranged by size:
1097 1017 747 432 344 268 99
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 1309 2876 1309
Size: 1567 2437
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 260 932 1008 3357 3613 260
Size: 672 76 2349 256 651
Fragments arranged by size:
2349 672 651 256 76
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 1024 1024
Size: 4004
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 145 679 1020 1434 1682 1717 145
Size: 534 341 414 248 35 2432
Fragments arranged by size:
2432 534 414 341 248 35
BanII G_rGCy'C
Cuts at: 1018 2048 1018
Size: 1030 2974
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BbsI GAAGACnn'nnnn_
Cuts at: 295 295
Size: 4004
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 738 1524 738
Size: 786 3218
BglII A'GATC_T
Cuts at: 616 1519 3556 616
Size: 903 2037 1064
Fragments arranged by size:
2037 1064 903
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 772 1629 1722 2778 772
Size: 857 93 1056 1998
Fragments arranged by size:
1998 1056 857 93
BmrI ACTGGGnnnn_n'
Cuts at: 1216 1297 1632 3776 1216
Size: 81 335 2144 1444
Fragments arranged by size:
2144 1444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 641 605
Size: 36 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 633 1429 2187 2764 3260 633
Size: 796 758 577 496 1377
Fragments arranged by size:
1377 796 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1796 1986 2778 1018
Size: 778 190 792 2244
Fragments arranged by size:
2244 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 772 1018 1629 1722 1796 1986 2048 2778 772
Size: 246 611 93 74 190 62 730 1998
Fragments arranged by size:
1998 730 611 246 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1054 1268 1570 1951 1054
Size: 214 302 381 3107
Fragments arranged by size:
3107 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstYI r'GATC_y
Cuts at: 1 363 616 1029 1496 1519 1854 2100 3112
Size: 362 253 413 467 23 335 246 1012
Cuts at: 3112 3198 3210 3556 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 413 362 346 335 253
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 359 720 107
Size: 252 361 3391
Fragments arranged by size:
3391 361 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1247
Size: 4004
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 790 1589 1763 2154 2181 3526 790
Size: 799 174 391 27 1345 1268
Fragments arranged by size:
1345 1268 799 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 221 468 751 2027 2237 221
Size: 247 283 1276 210 1988
Fragments arranged by size:
1988 1276 283 247 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 388 605 641 732 1829 2261 3008 3276
Size: 99 217 36 91 1097 432 747 268
Cuts at: 3276 289
Size: 1017
Fragments arranged by size:
1097 1017 747 432 268 217 99 91
36
EcoO109I rG'GnC_Cy
Cuts at: 1143 1143
Size: 4004
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 393 2023 393
Size: 1630 2374
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 883 915 1846 1876 1878 1908 883
Size: 32 931 30 2 30 2979
Fragments arranged by size:
2979 931 32 30 30 2
HincII GTy'rAC
Cuts at: 663 911 1043 663
Size: 248 132 3624
Fragments arranged by size:
3624 248 132
HpaI GTT'AAC
Cuts at: 911 911
Size: 4004
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 149 1024 149
Size: 875 3129
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 792 1765 792
Size: 973 3031
MslI CAynn'nnrTG
Cuts at: 1993 2120 1993
Size: 127 3877
MspA1I CmG'CkG
Cuts at: 1115 1429 1789 2802 3047 1115
Size: 314 360 1013 245 2072
Fragments arranged by size:
2072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 107 359 720 107
Size: 252 361 3391
Fragments arranged by size:
3391 361 252
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 1057 1557 2464 1057
Size: 500 907 2597
Fragments arranged by size:
2597 907 500
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 1429 1789 1429
Size: 360 3644
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 246 3521 246
Size: 3275 729
SfcI C'TryA_G
Cuts at: 33 182 445 1047 1732 2725 2916 33
Size: 149 263 602 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 263 191 149
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 1159 2074 2566 2828 1159
Size: 915 492 262 2335
Fragments arranged by size:
2335 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StyI C'CwwG_G
Cuts at: 107 359 720 1138 1492 107
Size: 252 361 418 354 2619
Fragments arranged by size:
2619 418 361 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 244 401 3519 244
Size: 157 3118 729
Fragments arranged by size:
3118 729 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 3972
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 469 1035 469
Size: 566 3438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 665 1499 665
Size: 834 3170
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
XmnI GAAnn'nnTTC
Cuts at: 236 236
Size: 4004
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI