Restriction map for
pF1KB8462
(Circular) (Six-base) MAPSORT of: pF1KB8462.seq Check: 1057 from: 1 to: 4241
pF1KB8462 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 960 1257 960
Size: 297 3944
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 2851 3513 2066
Size: 432 353 662 2794
Fragments arranged by size:
2794 662 432 353
AflIII A'CryG_T
Cuts at: 669 1593 3393 3674 669
Size: 924 1800 281 1236
Fragments arranged by size:
1800 1236 924 281
AgeI A'CCGG_T
Cuts at: 268 268
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1099 1546 2984 1099
Size: 447 1438 2356
Fragments arranged by size:
2356 1438 447
ApaLI G'TGCA_C
Cuts at: 3079 3079
Size: 4241
ApoI r'AATT_y
Cuts at: 218 414 1245 3594 3850 218
Size: 196 831 2349 256 609
Fragments arranged by size:
2349 831 609 256 196
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
AvrII C'CTAG_G
Cuts at: 792 792
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 237 622 960 1257 1671 1919 1954 237
Size: 385 338 297 414 248 35 2524
Fragments arranged by size:
2524 414 385 338 297 248 35
BanII G_rGCy'C
Cuts at: 915 1255 2285 915
Size: 340 1030 2871
Fragments arranged by size:
2871 1030 340
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 426 460 568 602 426
Size: 34 108 34 4065
Fragments arranged by size:
4065 108 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2139 3195 4241 2139
Size: 1056 1046 2139
Fragments arranged by size:
2139 1056 1046
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglI GCCn_nnn'nGGC
Cuts at: 892 892
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 815 1866 1959 3083 815
Size: 1051 93 1124 1973
Fragments arranged by size:
1973 1124 1051 93
BmrI ACTGGGnnnn_n'
Cuts at: 721 1453 1534 1869 4013 721
Size: 732 81 335 2144 949
Fragments arranged by size:
2144 949 732 335 81
BmtI G_CTAG'C
Cuts at: 1175 1175
Size: 4241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 1417 2296 3010 3308 233
Size: 1184 879 714 298 1166
Fragments arranged by size:
1184 1166 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 670 1594 670
Size: 924 3317
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 268 750 1430 1951 2629 3040 3187 3461 268
Size: 482 680 521 678 411 147 274 1048
Fragments arranged by size:
1048 680 678 521 482 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 141 171 141
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 923 923
Size: 4241
BseYI C'CCAG_C
Cuts at: 334 1666 2424 3089 3497 334
Size: 1332 758 665 408 1078
Fragments arranged by size:
1332 1078 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 968
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3059 104
Size: 1679 46 1230 1286
Fragments arranged by size:
1679 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 375 1255 2033 2223 3083 375
Size: 880 778 190 860 1533
Fragments arranged by size:
1533 880 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 375 815 915 1255 1866 1959 2033 2223 2285
Size: 440 100 340 611 93 74 190 62
Cuts at: 2285 3083 375
Size: 798 1533
Fragments arranged by size:
1533 798 611 440 340 190 100 93
74 62
BspEI T'CCGG_A
Cuts at: 750 750
Size: 4241
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 958 2533 2587 4071 4236 50
Size: 908 1575 54 1484 165 55
Fragments arranged by size:
1575 1484 908 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 804 2153 173
Size: 631 1349 2261
Fragments arranged by size:
2261 1349 631
BsrFI r'CCGG_y
Cuts at: 259 268 2239 2420 259
Size: 9 1971 181 2080
Fragments arranged by size:
2080 1971 181 9
BsrGI T'GTAC_A
Cuts at: 1133 1133
Size: 4241
BssSI C'ACGA_G
Cuts at: 2512 3220 2512
Size: 708 3533
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1099 1618 1063
Size: 36 519 3686
Fragments arranged by size:
3686 519 36
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstEII G'GTnAC_C
Cuts at: 136 136
Size: 4241
BstYI r'GATC_y
Cuts at: 1 349 1266 1733 1756 2091 2337 2643 2655
Size: 348 917 467 23 335 246 306 12
Cuts at: 2655 2741 3793 1
Size: 86 1052 449
Fragments arranged by size:
1052 917 467 449 348 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 309 626 890 107
Size: 202 317 264 3458
Fragments arranged by size:
3458 317 264 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 815 1484 815
Size: 669 3572
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 3291 1947
Size: 1344 2897
EaeI y'GGCC_r
Cuts at: 261 384 1826 2000 2391 2418 3763 261
Size: 123 1442 174 391 27 1345 739
Fragments arranged by size:
1442 1345 739 391 174 123 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 819 2264 2474 819
Size: 1445 210 2586
Fragments arranged by size:
2586 1445 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 948 2449 3191 3337 4051 948
Size: 1501 742 146 714 1138
Fragments arranged by size:
1501 1138 742 714 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 2851 3513 2066
Size: 432 353 662 2794
Fragments arranged by size:
2794 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 477 1380 477
Size: 903 3338
EcoRI G'AATT_C
Cuts at: 218 1245 218
Size: 1027 3214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1190 1675 1923 3153 3924 1190
Size: 485 248 1230 771 1507
Fragments arranged by size:
1507 1230 771 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 141 173 412 444 818 850 2083 2113 2115
Size: 32 239 32 374 32 1233 30 2
Cuts at: 2115 2145 141
Size: 30 2237
Fragments arranged by size:
2237 1233 374 239 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 964 1261 964
Size: 297 3944
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 694 714 991 3000 3184 694
Size: 20 277 2009 184 1751
Fragments arranged by size:
2009 1751 277 184 20
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 2230 2357 2230
Size: 127 4114
MspA1I CmG'CkG
Cuts at: 1087 1099 1352 1666 2026 2810 3055 1087
Size: 12 253 314 360 784 245 2273
Fragments arranged by size:
2273 784 360 314 253 245 12
NaeI GCC'GGC
Cuts at: 261 2422 261
Size: 2161 2080
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 107 107
Size: 4241
NdeI CA'TA_TG
Cuts at: 702 702
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 259 2420 259
Size: 2161 2080
NheI G'CTAG_C
Cuts at: 1171 1171
Size: 4241
NspI r_CATG'y
Cuts at: 451 1294 1794 3397 451
Size: 843 500 1603 1295
Fragments arranged by size:
1603 1295 843 500
PciI A'CATG_T
Cuts at: 3393 3393
Size: 4241
PflMI CCAn_nnn'nTGG
Cuts at: 733 733
Size: 4241
PfoI T'CCnGG_A
Cuts at: 473 473
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PmlI CAC'GTG
Cuts at: 670 670
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 477 477
Size: 4241
PstI C_TGCA'G
Cuts at: 885 1095 1104 1288 1973 885
Size: 210 9 184 685 3153
Fragments arranged by size:
3153 685 210 184 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 1666 2026 1666
Size: 360 3881
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SanDI GG'GwC_CC
Cuts at: 477 477
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 662 881 1047 1091 1100 1215 1284 1969
Size: 629 219 166 44 9 115 69 685
Cuts at: 1969 2937 3128 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 629 219 191 166 115
69 44 9
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 248 1396 2311 3025 3287 248
Size: 1148 915 714 262 1202
Fragments arranged by size:
1202 1148 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
SspI AAT'ATT
Cuts at: 459 459
Size: 4241
StyI C'CwwG_G
Cuts at: 107 792 1375 1729 107
Size: 685 583 354 2619
Fragments arranged by size:
2619 685 583 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 1824 1957 2416 2625 348
Size: 1476 133 459 209 1964
Fragments arranged by size:
1964 1476 459 209 133
TatI w'GTAC_w
Cuts at: 1133 3756 1133
Size: 2623 1618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 831 863 3758 3790 831
Size: 32 2895 32 1282
Fragments arranged by size:
2895 1282 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 687 1736 687
Size: 1049 3192
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BfrBI BmgBI BpmI
Bpu10I BsiWI BsmI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI