Restriction map for
pF1KB8459
(Circular) (Six-base) MAPSORT of: pF1KB8459.seq Check: 326 from: 1 to: 4517
pF1KB8459 4517 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1555 1555
Size: 4517
Acc65I G'GTAC_C
Cuts at: 303 1533 303
Size: 1230 3287
AclI AA'CG_TT
Cuts at: 811 1328 1376 4357 811
Size: 517 48 2981 971
Fragments arranged by size:
2981 971 517 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 857 2342 2774 3127 3789 191
Size: 666 1485 432 353 662 919
Fragments arranged by size:
1485 919 666 662 432 353
AflIII A'CryG_T
Cuts at: 1869 3669 3950 1869
Size: 1800 281 2436
Fragments arranged by size:
2436 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1336 1336
Size: 4517
AlwNI CAG_nnn'CTG
Cuts at: 1822 3260 1822
Size: 1438 3079
ApaLI G'TGCA_C
Cuts at: 3355 3355
Size: 4517
ApoI r'AATT_y
Cuts at: 726 1487 1521 3870 4126 726
Size: 761 34 2349 256 1117
Fragments arranged by size:
2349 1117 761 256 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4517
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 1537 1537
Size: 4517
BamHI G'GATC_C
Cuts at: 1542 1542
Size: 4517
BanI G'GyrC_C
Cuts at: 303 1533 1947 2195 2230 303
Size: 1230 414 248 35 2590
Fragments arranged by size:
2590 1230 414 248 35
BanII G_rGCy'C
Cuts at: 958 1531 2561 958
Size: 573 1030 2914
Fragments arranged by size:
2914 1030 573
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 1282 1282
Size: 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1402 1436 1402
Size: 34 4483
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3471 4517 2415
Size: 1056 1046 2415
Fragments arranged by size:
2415 1056 1046
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BglII A'GATC_T
Cuts at: 2032 4069 2032
Size: 2037 2480
BlpI GC'TnA_GC
Cuts at: 1629 1629
Size: 4517
Bme1580I G_kGCm'C
Cuts at: 399 2142 2235 3359 399
Size: 1743 93 1124 1557
Fragments arranged by size:
1743 1557 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1729 1810 2145 4289 1729
Size: 81 335 2144 1957
Fragments arranged by size:
2144 1957 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 332 368 990 1516 290
Size: 42 36 622 526 3291
Fragments arranged by size:
3291 622 526 42 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2572 3286 3584 1693
Size: 879 714 298 2626
Fragments arranged by size:
2626 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4221 4221
Size: 4517
BsaAI yAC'GTr
Cuts at: 630 1870 630
Size: 1240 3277
BsaBI GATnn'nnATC
Cuts at: 6 258 2031 4068 6
Size: 252 1773 2037 455
Fragments arranged by size:
2037 1773 455 252
BsaHI Gr'CG_yC
Cuts at: 1948 2196 2898 4394 1948
Size: 248 702 1496 2071
Fragments arranged by size:
2071 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1706 2227 2905 3316 3463 3737 1706
Size: 521 678 411 147 274 2486
Fragments arranged by size:
2486 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 753 783 753
Size: 30 4487
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 287 329 519 522 287
Size: 42 190 3 4282
Fragments arranged by size:
4282 190 42 3
BseYI C'CCAG_C
Cuts at: 1942 2700 3365 3773 1942
Size: 758 665 408 2686
Fragments arranged by size:
2686 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2059 2105 3335 104
Size: 1955 46 1230 1286
Fragments arranged by size:
1955 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1531 2309 2499 3359 1531
Size: 778 190 860 2689
Fragments arranged by size:
2689 860 778 190
BsmI GAATG_Cn'
Cuts at: 1108 1425 1108
Size: 317 4200
Bsp1286I G_dGCh'C
Cuts at: 399 958 1531 2142 2235 2309 2499 2561 3359
Size: 559 573 611 93 74 190 62 798
Cuts at: 3359 399
Size: 1557
Fragments arranged by size:
1557 798 611 573 559 190 93 74
62
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1567 1781 2083 2464 1567
Size: 214 302 381 3620
Fragments arranged by size:
3620 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2809 2863 4347 4512 50
Size: 2759 54 1484 165 55
Fragments arranged by size:
2759 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2429 2429
Size: 4517
BsrFI r'CCGG_y
Cuts at: 2515 2696 2515
Size: 181 4336
BssSI C'ACGA_G
Cuts at: 2788 3496 2788
Size: 708 3809
BstAPI GCAn_nnn'nTGC
Cuts at: 1894 1894
Size: 4517
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstYI r'GATC_y
Cuts at: 1 429 1542 2009 2032 2367 2613 2919 2931
Size: 428 1113 467 23 335 246 306 12
Cuts at: 2931 3017 4069 1
Size: 86 1052 449
Fragments arranged by size:
1113 1052 467 449 428 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 191 107
Size: 84 4433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2467 2648 2811 2467
Size: 181 163 4173
Fragments arranged by size:
4173 181 163
BtsI GCAGTG_nn'
Cuts at: 1760 1760
Size: 4517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 462 497 462
Size: 35 4482
DraI TTT'AAA
Cuts at: 282 291 669 1227 1256 1516 282
Size: 9 378 558 29 260 3283
Fragments arranged by size:
3283 558 378 260 29 9
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3567 2223
Size: 1344 3173
EaeI y'GGCC_r
Cuts at: 110 194 587 2102 2276 2667 2694 4039 110
Size: 84 393 1515 174 391 27 1345 588
Fragments arranged by size:
1515 1345 588 393 391 174 84 27
EagI C'GGCC_G
Cuts at: 2102 2102
Size: 4517
EarI CTCTTCn'nnn_
Cuts at: 167 1053 1118 2540 2750 167
Size: 886 65 1422 210 1934
Fragments arranged by size:
1934 1422 886 210 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2725 3467 3613 4327 2725
Size: 742 146 714 2915
Fragments arranged by size:
2915 742 714 146
EcoICRI GAG'CTC
Cuts at: 1529 1529
Size: 4517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 290 332 368 857 990 1516 2342 2774
Size: 99 42 36 489 133 526 826 432
Cuts at: 2774 3127 3789 191
Size: 353 662 919
Fragments arranged by size:
919 826 662 526 489 432 353 133
99 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 311 362 311
Size: 51 4466
EcoO109I rG'GnC_Cy
Cuts at: 1656 1656
Size: 4517
EcoRI G'AATT_C
Cuts at: 726 1521 726
Size: 795 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 459 2536 459
Size: 2077 2440
FspI TGC'GCA
Cuts at: 2298 2298
Size: 4517
HaeII r_GCGC'y
Cuts at: 347 1951 2199 3429 4200 347
Size: 1604 248 1230 771 664
Fragments arranged by size:
1604 1230 771 664 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 877 885 909 2359 2389 2391 2421 853
Size: 24 8 24 1450 30 2 30 2949
Fragments arranged by size:
2949 1450 30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 809 1556 809
Size: 747 3770
HindIII A'AGCT_T
Cuts at: 554 554
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 307 1537 307
Size: 1230 3287
MfeI C'AATT_G
Cuts at: 1406 1406
Size: 4517
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 231 543 3276 3460 231
Size: 312 2733 184 1288
Fragments arranged by size:
2733 1288 312 184
MscI TGG'CCA
Cuts at: 112 196 2278 112
Size: 84 2082 2351
Fragments arranged by size:
2351 2082 84
MslI CAynn'nnrTG
Cuts at: 2506 2633 2506
Size: 127 4390
MspA1I CmG'CkG
Cuts at: 424 757 1628 1942 2302 3086 3331 424
Size: 333 871 314 360 784 245 1610
Fragments arranged by size:
1610 871 784 360 333 314 245
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 4517
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 107 191 107
Size: 84 4433
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 4517
NspI r_CATG'y
Cuts at: 1570 2070 3673 1570
Size: 500 1603 2414
Fragments arranged by size:
2414 1603 500
PciI A'CATG_T
Cuts at: 3669 3669
Size: 4517
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PmlI CAC'GTG
Cuts at: 630 630
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 4485
PstI C_TGCA'G
Cuts at: 1564 2249 1564
Size: 685 3832
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 757 1942 2302 757
Size: 1185 360 2972
Fragments arranged by size:
2972 1185 360
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 1531 1531
Size: 4517
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SfcI C'TryA_G
Cuts at: 33 1262 1560 2245 3213 3404 33
Size: 1229 298 685 968 191 1146
Fragments arranged by size:
1229 1146 968 685 298 191
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 1539 1539
Size: 4517
SmlI C'TyrA_G
Cuts at: 1672 2587 3301 3563 1672
Size: 915 714 262 2626
Fragments arranged by size:
2626 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1570 2070 1570
Size: 500 4017
SspI AAT'ATT
Cuts at: 685 685
Size: 4517
StyI C'CwwG_G
Cuts at: 107 191 434 476 1651 2005 107
Size: 84 243 42 1175 354 2619
Fragments arranged by size:
2619 1175 354 243 84 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1356 2100 2233 2692 2901 1155
Size: 201 744 133 459 209 2771
Fragments arranged by size:
2771 744 459 209 201 133
TatI w'GTAC_w
Cuts at: 4032 4032
Size: 4517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4034 4066 4034
Size: 32 4485
Tth111I GACn'n_nGTC
Cuts at: 2314 2314
Size: 4517
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1068 2012 1068
Size: 944 3573
XmaI C'CCGG_G
Cuts at: 1537 1537
Size: 4517
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI