Restriction map for
pF1KB8455
(Circular) (Six-base) MAPSORT of: pF1KB8455.seq Check: 6023 from: 1 to: 3458
pF1KB8455 3458 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 392 496 392
Size: 104 3354
Acc65I G'GTAC_C
Cuts at: 474 474
Size: 3458
AclI AA'CG_TT
Cuts at: 3298 3298
Size: 3458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 1715 2068 2730 1283
Size: 432 353 662 2011
Fragments arranged by size:
2011 662 432 353
AflIII A'CryG_T
Cuts at: 810 2610 2891 810
Size: 1800 281 1377
Fragments arranged by size:
1800 1377 281
AleI CACnn'nnGTG
Cuts at: 133 133
Size: 3458
AlwNI CAG_nnn'CTG
Cuts at: 186 763 2201 186
Size: 577 1438 1443
Fragments arranged by size:
1443 1438 577
ApaLI G'TGCA_C
Cuts at: 226 2296 226
Size: 2070 1388
ApoI r'AATT_y
Cuts at: 462 2811 3067 462
Size: 2349 256 853
Fragments arranged by size:
2349 853 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3458
AvaI C'yCGr_G
Cuts at: 478 478
Size: 3458
BamHI G'GATC_C
Cuts at: 483 483
Size: 3458
BanI G'GyrC_C
Cuts at: 474 888 1136 1171 474
Size: 414 248 35 2761
Fragments arranged by size:
2761 414 248 35
BanII G_rGCy'C
Cuts at: 176 472 1502 176
Size: 296 1030 2132
Fragments arranged by size:
2132 1030 296
BbeI G_GCGC'C
Cuts at: 892 1140 892
Size: 248 3210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3424
BciVI GTATCCnnnnn_n'
Cuts at: 1356 2412 3458 1356
Size: 1056 1046 1356
Fragments arranged by size:
1356 1056 1046
BclI T'GATC_A
Cuts at: 978 978
Size: 3458
BglII A'GATC_T
Cuts at: 973 3010 973
Size: 2037 1421
BlpI GC'TnA_GC
Cuts at: 570 570
Size: 3458
Bme1580I G_kGCm'C
Cuts at: 140 230 1083 1176 2300 140
Size: 90 853 93 1124 1298
Fragments arranged by size:
1298 1124 853 93 90
BmrI ACTGGGnnnn_n'
Cuts at: 207 670 751 1086 3230 207
Size: 463 81 335 2144 435
Fragments arranged by size:
2144 463 435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 249
Size: 3458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1513 2227 2525 634
Size: 879 714 298 1567
Fragments arranged by size:
1567 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3162 3162
Size: 3458
BsaAI yAC'GTr
Cuts at: 231 811 231
Size: 580 2878
BsaBI GATnn'nnATC
Cuts at: 6 118 972 3009 6
Size: 112 854 2037 455
Fragments arranged by size:
2037 854 455 112
BsaHI Gr'CG_yC
Cuts at: 889 1137 1839 3335 889
Size: 248 702 1496 1012
Fragments arranged by size:
1496 1012 702 248
BsaWI w'CCGG_w
Cuts at: 647 1168 1846 2257 2404 2678 647
Size: 521 678 411 147 274 1427
Fragments arranged by size:
1427 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 179 883 1641 2306 2714 179
Size: 704 758 665 408 923
Fragments arranged by size:
923 758 704 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 277
Size: 3458
BsiEI CG_ry'CG
Cuts at: 104 1000 1046 2276 104
Size: 896 46 1230 1286
Fragments arranged by size:
1286 1230 896 46
BsiHKAI G_wGCw'C
Cuts at: 176 230 472 1250 1440 2300 176
Size: 54 242 778 190 860 1334
Fragments arranged by size:
1334 860 778 242 190 54
Bsp1286I G_dGCh'C
Cuts at: 140 176 230 472 1083 1176 1250 1440 1502
Size: 36 54 242 611 93 74 190 62
Cuts at: 1502 2300 140
Size: 798 1298
Fragments arranged by size:
1298 798 611 242 190 93 74 62
54 36
BspHI T'CATG_A
Cuts at: 1890 1890
Size: 3458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 508 722 1024 1405 508
Size: 214 302 381 2561
Fragments arranged by size:
2561 381 302 214
BsrBI CCG'CTC
Cuts at: 50 329 1750 1804 3288 3453 50
Size: 279 1421 54 1484 165 55
Fragments arranged by size:
1484 1421 279 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1370 1370
Size: 3458
BsrFI r'CCGG_y
Cuts at: 199 1456 1637 199
Size: 1257 181 2020
Fragments arranged by size:
2020 1257 181
BssHII G'CGCG_C
Cuts at: 278 317 278
Size: 39 3419
BssSI C'ACGA_G
Cuts at: 1729 2437 1729
Size: 708 2750
BstAPI GCAn_nnn'nTGC
Cuts at: 835 835
Size: 3458
BstBI TT'CG_AA
Cuts at: 1819 1819
Size: 3458
BstYI r'GATC_y
Cuts at: 1 483 950 973 1308 1554 1860 1872 1958
Size: 482 467 23 335 246 306 12 86
Cuts at: 1958 3010 1
Size: 1052 449
Fragments arranged by size:
1052 482 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 204 1408 1589 1752 204
Size: 1204 181 163 1910
Fragments arranged by size:
1910 1204 181 163
BtsI GCAGTG_nn'
Cuts at: 701 701
Size: 3458
DraI TTT'AAA
Cuts at: 457 457
Size: 3458
DrdI GACnn_nn'nnGTC
Cuts at: 1164 2508 1164
Size: 1344 2114
EaeI y'GGCC_r
Cuts at: 359 419 1043 1217 1608 1635 2980 359
Size: 60 624 174 391 27 1345 837
Fragments arranged by size:
1345 837 624 391 174 60 27
EagI C'GGCC_G
Cuts at: 1043 1043
Size: 3458
EarI CTCTTCn'nnn_
Cuts at: 1481 1691 1481
Size: 210 3248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1666 2408 2554 3268 1666
Size: 742 146 714 1856
Fragments arranged by size:
1856 742 714 146
EcoICRI GAG'CTC
Cuts at: 174 470 174
Size: 296 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 1283 1715 2068 2730 249
Size: 1034 432 353 662 977
Fragments arranged by size:
1034 977 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 597 597
Size: 3458
EcoRI G'AATT_C
Cuts at: 462 462
Size: 3458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1477 1477
Size: 3458
FspI TGC'GCA
Cuts at: 373 1239 373
Size: 866 2592
HaeII r_GCGC'y
Cuts at: 369 892 1140 2370 3141 369
Size: 523 248 1230 771 686
Fragments arranged by size:
1230 771 686 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1300 1330 1332 1362 1300
Size: 30 2 30 3396
Fragments arranged by size:
3396 30 30 2
HincII GTy'rAC
Cuts at: 497 497
Size: 3458
KasI G'GCGC_C
Cuts at: 888 1136 888
Size: 248 3210
KpnI G_GTAC'C
Cuts at: 478 478
Size: 3458
MluI A'CGCG_T
Cuts at: 2891 2891
Size: 3458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2217 2401 2217
Size: 184 3274
MscI TGG'CCA
Cuts at: 1219 1219
Size: 3458
MslI CAynn'nnrTG
Cuts at: 112 133 223 1447 1574 112
Size: 21 90 1224 127 1996
Fragments arranged by size:
1996 1224 127 90 21
MspA1I CmG'CkG
Cuts at: 186 569 883 1243 2027 2272 186
Size: 383 314 360 784 245 1372
Fragments arranged by size:
1372 784 383 360 314 245
NaeI GCC'GGC
Cuts at: 1639 1639
Size: 3458
NarI GG'CG_CC
Cuts at: 889 1137 889
Size: 248 3210
NgoMIV G'CCGG_C
Cuts at: 1637 1637
Size: 3458
NspI r_CATG'y
Cuts at: 511 1011 2614 511
Size: 500 1603 1355
Fragments arranged by size:
1603 1355 500
PciI A'CATG_T
Cuts at: 2610 2610
Size: 3458
PflMI CCAn_nnn'nTGG
Cuts at: 186 249 186
Size: 63 3395
PfoI T'CCnGG_A
Cuts at: 236 236
Size: 3458
PmeI GTTT'AAAC
Cuts at: 457 457
Size: 3458
PmlI CAC'GTG
Cuts at: 231 231
Size: 3458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1904 1936 1904
Size: 32 3426
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 316 348 316
Size: 32 3426
PstI C_TGCA'G
Cuts at: 289 505 1190 289
Size: 216 685 2557
Fragments arranged by size:
2557 685 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3458
PvuII CAG'CTG
Cuts at: 186 883 1243 186
Size: 697 360 2401
Fragments arranged by size:
2401 697 360
RsrII CG'GwC_CG
Cuts at: 1653 1653
Size: 3458
SacI G_AGCT'C
Cuts at: 176 472 176
Size: 296 3162
SalI G'TCGA_C
Cuts at: 495 495
Size: 3458
SapI GCTCTTCn'nnn_
Cuts at: 1481 1691 1481
Size: 210 3248
SbfI CC_TGCA'GG
Cuts at: 505 505
Size: 3458
ScaI AGT'ACT
Cuts at: 2975 2975
Size: 3458
SfcI C'TryA_G
Cuts at: 33 285 333 501 1186 2154 2345 33
Size: 252 48 168 685 968 191 1146
Fragments arranged by size:
1146 968 685 252 191 168 48
SfoI GGC'GCC
Cuts at: 890 1138 890
Size: 248 3210
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3458
SmaI CCC'GGG
Cuts at: 480 480
Size: 3458
SmlI C'TyrA_G
Cuts at: 613 1528 2242 2504 613
Size: 915 714 262 1567
Fragments arranged by size:
1567 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3458
SphI G_CATG'C
Cuts at: 511 1011 511
Size: 500 2958
StyI C'CwwG_G
Cuts at: 592 946 592
Size: 354 3104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 1041 1174 1633 1842 193
Size: 848 133 459 209 1809
Fragments arranged by size:
1809 848 459 209 133
TatI w'GTAC_w
Cuts at: 215 2973 215
Size: 2758 700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3426
Tth111I GACn'n_nGTC
Cuts at: 388 1255 388
Size: 867 2591
XbaI T'CTAG_A
Cuts at: 489 489
Size: 3458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 953 953
Size: 3458
XmaI C'CCGG_G
Cuts at: 478 478
Size: 3458
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BspEI BsrGI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI