Restriction map for
pF1KB8452
(Circular) (Six-base) MAPSORT of: pF1KB8452.seq Check: 2807 from: 1 to: 4184
pF1KB8452 4184 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 647 1200 647
Size: 553 3631
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 559 598 802 2009 2441 3188 3456 541
Size: 18 39 204 1207 432 747 268 1269
Fragments arranged by size:
1269 1207 747 432 268 204 39 18
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AhdI GACnn_n'nnGTC
Cuts at: 705 705
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 519 834 1489 3056 519
Size: 315 655 1567 1647
Fragments arranged by size:
1647 1567 655 315
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 1188 3537 3793 1188
Size: 2349 256 1579
Fragments arranged by size:
2349 1579 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 210 658 1204 210
Size: 448 546 3190
Fragments arranged by size:
3190 546 448
BamHI G'GATC_C
Cuts at: 494 1209 494
Size: 715 3469
BanI G'GyrC_C
Cuts at: 214 445 647 1200 1614 1862 1897 214
Size: 231 202 553 414 248 35 2501
Fragments arranged by size:
2501 553 414 248 231 202 35
BanII G_rGCy'C
Cuts at: 115 211 657 1040 1198 2228 115
Size: 96 446 383 158 1030 2071
Fragments arranged by size:
2071 1030 446 383 158 96
BbeI G_GCGC'C
Cuts at: 218 1618 1866 218
Size: 1400 248 2536
Fragments arranged by size:
2536 1400 248
BbsI GAAGACnn'nnnn_
Cuts at: 572 572
Size: 4184
BbvCI CC'TCA_GC
Cuts at: 529 529
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 734 1704 734
Size: 970 3214
BglI GCCn_nnn'nGGC
Cuts at: 254 607 1140 254
Size: 353 533 3298
Fragments arranged by size:
3298 533 353
BglII A'GATC_T
Cuts at: 1699 3736 1699
Size: 2037 2147
BlpI GC'TnA_GC
Cuts at: 1123 1296 1123
Size: 173 4011
Bme1580I G_kGCm'C
Cuts at: 448 1809 1902 2958 448
Size: 1361 93 1056 1674
Fragments arranged by size:
1674 1361 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 635 1396 1477 1812 3956 635
Size: 761 81 335 2144 863
Fragments arranged by size:
2144 863 761 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 961
Size: 4184
Bpu10I CC'TnA_GC
Cuts at: 260 529 260
Size: 269 3915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1360 2239 2731 3029 325
Size: 1035 879 492 298 1480
Fragments arranged by size:
1480 1035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 391 1164 3888 391
Size: 773 2724 687
Fragments arranged by size:
2724 773 687
BsaAI yAC'GTr
Cuts at: 633 1537 633
Size: 904 3280
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 215 808 1171 1615 1863 2565 4061 215
Size: 593 363 444 248 702 1496 338
Fragments arranged by size:
1496 702 593 444 363 338 248
BsaWI w'CCGG_w
Cuts at: 491 1373 1894 2572 2846 2993 3404 491
Size: 882 521 678 274 147 411 1271
Fragments arranged by size:
1271 882 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1146 1609 2367 2944 3440 1146
Size: 463 758 577 496 1890
Fragments arranged by size:
1890 758 577 496 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1003 847
Size: 156 4028
BsiEI CG_ry'CG
Cuts at: 104 239 249 1726 1772 2980 104
Size: 135 10 1477 46 1208 1308
Fragments arranged by size:
1477 1308 1208 135 46 10
BsiHKAI G_wGCw'C
Cuts at: 115 235 1124 1198 1976 2166 2958 115
Size: 120 889 74 778 190 792 1341
Fragments arranged by size:
1341 889 792 778 190 120 74
BsmBI CGTCTCn'nnnn_
Cuts at: 1162 1162
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 115 211 235 448 657 1040 1124 1198 1809
Size: 96 24 213 209 383 84 74 611
Cuts at: 1809 1902 1976 2166 2228 2958 115
Size: 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 383 213 209 190 96
93 84 74 74 62 24
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 464 791 3360 464
Size: 327 2569 1288
Fragments arranged by size:
2569 1288 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 203 1234 1448 1750 2131 203
Size: 1031 214 302 381 2256
Fragments arranged by size:
2256 1031 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 253 2182 2363 2572 253
Size: 1929 181 209 1865
Fragments arranged by size:
1929 1865 209 181
BssHII G'CGCG_C
Cuts at: 177 315 177
Size: 138 4046
BssSI C'ACGA_G
Cuts at: 355 505 2455 2813 355
Size: 150 1950 358 1726
Fragments arranged by size:
1950 1726 358 150
BstAPI GCAn_nnn'nTGC
Cuts at: 834 1561 834
Size: 727 3457
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstEII G'GTnAC_C
Cuts at: 153 636 153
Size: 483 3701
BstYI r'GATC_y
Cuts at: 1 494 581 1209 1676 1699 2034 2280 3292
Size: 493 87 628 467 23 335 246 1012
Cuts at: 3292 3378 3390 3736 1
Size: 86 12 346 449
Fragments arranged by size:
1012 628 493 467 449 346 335 246
87 86 23 12
BtgI C'CryG_G
Cuts at: 203 239 710 203
Size: 36 471 3677
Fragments arranged by size:
3677 471 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 857 2134 2315 2478 857
Size: 1277 181 163 2563
Fragments arranged by size:
2563 1277 181 163
BtsI GCAGTG_nn'
Cuts at: 138 1427 138
Size: 1289 2895
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 236 246 266 599 1769 1943 2334 2361 3706
Size: 10 20 333 1170 174 391 27 1345
Cuts at: 3706 236
Size: 714
Fragments arranged by size:
1345 1170 714 391 333 174 27 20
10
EagI C'GGCC_G
Cuts at: 236 246 1769 236
Size: 10 1523 2651
Fragments arranged by size:
2651 1523 10
EarI CTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 107 742 2392 2702 2848 3994 107
Size: 635 1650 310 146 1146 297
Fragments arranged by size:
1650 1146 635 310 297 146
EcoICRI GAG'CTC
Cuts at: 113 1196 113
Size: 1083 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 559 598 802 961 2009 2441 3188 3456
Size: 18 39 204 159 1048 432 747 268
Cuts at: 3456 541
Size: 1269
Fragments arranged by size:
1269 1048 747 432 268 204 159 39
18
EcoO109I rG'GnC_Cy
Cuts at: 192 534 867 1323 192
Size: 342 333 456 3053
Fragments arranged by size:
3053 456 342 333
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 218 260 994 1618 1866 2888 3867 218
Size: 42 734 624 248 1022 979 535
Fragments arranged by size:
1022 979 734 624 535 248 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 691 723 883 915 2026 2056 2058 2088 691
Size: 32 160 32 1111 30 2 30 2787
Fragments arranged by size:
2787 1111 160 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1077 1223 1077
Size: 146 4038
KasI G'GCGC_C
Cuts at: 214 1614 1862 214
Size: 1400 248 2536
Fragments arranged by size:
2536 1400 248
KpnI G_GTAC'C
Cuts at: 651 1204 651
Size: 553 3631
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3039 2855
Size: 184 4000
MscI TGG'CCA
Cuts at: 268 601 1945 268
Size: 333 1344 2507
Fragments arranged by size:
2507 1344 333
MslI CAynn'nnrTG
Cuts at: 796 2173 2300 796
Size: 1377 127 2680
Fragments arranged by size:
2680 1377 127
MspA1I CmG'CkG
Cuts at: 780 831 972 1146 1295 1609 1969 2982 3227
Size: 51 141 174 149 314 360 1013 245
Cuts at: 3227 780
Size: 1737
Fragments arranged by size:
1737 1013 360 314 245 174 149 141
51
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 215 1615 1863 215
Size: 1400 248 2536
Fragments arranged by size:
2536 1400 248
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 351 1237 1737 2644 351
Size: 886 500 907 1891
Fragments arranged by size:
1891 907 886 500
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PfoI T'CCnGG_A
Cuts at: 584 938 584
Size: 354 3830
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 192 534 192
Size: 342 3842
PstI C_TGCA'G
Cuts at: 200 839 1231 1916 200
Size: 639 392 685 2468
Fragments arranged by size:
2468 685 639 392
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 780 1146 1609 1969 780
Size: 366 463 360 2995
Fragments arranged by size:
2995 463 366 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 115 1198 115
Size: 1083 3101
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SexAI A'CCwGG_T
Cuts at: 425 425
Size: 4184
SfcI C'TryA_G
Cuts at: 33 196 835 1227 1912 2905 3096 33
Size: 163 639 392 685 993 191 1121
Fragments arranged by size:
1121 993 685 639 392 191 163
SfoI GGC'GCC
Cuts at: 216 1616 1864 216
Size: 1400 248 2536
Fragments arranged by size:
2536 1400 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SgrAI Cr'CCGG_yG
Cuts at: 253 253
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 340 1339 2254 2746 3008 340
Size: 999 915 492 262 1516
Fragments arranged by size:
1516 999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 1163 1318 1672 1163
Size: 155 354 3675
Fragments arranged by size:
3675 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1767 1900 2359 2568 1767
Size: 133 459 209 3383
Fragments arranged by size:
3383 459 209 133
TatI w'GTAC_w
Cuts at: 3699 3699
Size: 4184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4152
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1679 1679
Size: 4184
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BsrGI BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI