Restriction map for
pF1KB8449
(Circular) (Six-base) MAPSORT of: pF1KB8449.seq Check: 9323 from: 1 to: 3518
pF1KB8449 3518 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 556 556
Size: 3518
Acc65I G'GTAC_C
Cuts at: 534 534
Size: 3518
AclI AA'CG_TT
Cuts at: 3358 3358
Size: 3518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1343 1775 2128 2790 1343
Size: 432 353 662 2071
Fragments arranged by size:
2071 662 432 353
AflIII A'CryG_T
Cuts at: 870 2670 2951 870
Size: 1800 281 1437
Fragments arranged by size:
1800 1437 281
AleI CACnn'nnGTG
Cuts at: 283 283
Size: 3518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 387 387
Size: 3518
AlwNI CAG_nnn'CTG
Cuts at: 823 2261 823
Size: 1438 2080
ApaLI G'TGCA_C
Cuts at: 2356 2356
Size: 3518
ApoI r'AATT_y
Cuts at: 424 522 2871 3127 424
Size: 98 2349 256 815
Fragments arranged by size:
2349 815 256 98
AseI AT'TA_AT
Cuts at: 20 20
Size: 3518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
AvaI C'yCGr_G
Cuts at: 538 538
Size: 3518
BamHI G'GATC_C
Cuts at: 543 543
Size: 3518
BanI G'GyrC_C
Cuts at: 534 948 1196 1231 534
Size: 414 248 35 2821
Fragments arranged by size:
2821 414 248 35
BanII G_rGCy'C
Cuts at: 532 1562 532
Size: 1030 2488
BbeI G_GCGC'C
Cuts at: 952 1200 952
Size: 248 3270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 363 397 363
Size: 34 3484
BciVI GTATCCnnnnn_n'
Cuts at: 412 1416 2472 3518 412
Size: 1004 1056 1046 412
Fragments arranged by size:
1056 1046 1004 412
BclI T'GATC_A
Cuts at: 1038 1038
Size: 3518
BglII A'GATC_T
Cuts at: 1033 3070 1033
Size: 2037 1481
BlpI GC'TnA_GC
Cuts at: 630 630
Size: 3518
Bme1580I G_kGCm'C
Cuts at: 1143 1236 2360 1143
Size: 93 1124 2301
Fragments arranged by size:
2301 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 730 811 1146 3290 730
Size: 81 335 2144 958
Fragments arranged by size:
2144 958 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1885 1885
Size: 3518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 472 170
Size: 302 3216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1573 2287 2585 694
Size: 879 714 298 1627
Fragments arranged by size:
1627 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3222 3222
Size: 3518
BsaAI yAC'GTr
Cuts at: 871 871
Size: 3518
BsaBI GATnn'nnATC
Cuts at: 6 1032 3069 6
Size: 1026 2037 455
Fragments arranged by size:
2037 1026 455
BsaHI Gr'CG_yC
Cuts at: 272 949 1197 1899 3395 272
Size: 677 248 702 1496 395
Fragments arranged by size:
1496 702 677 395 248
BsaWI w'CCGG_w
Cuts at: 707 1228 1906 2317 2464 2738 707
Size: 521 678 411 147 274 1487
Fragments arranged by size:
1487 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 943 1701 2366 2774 943
Size: 758 665 408 1687
Fragments arranged by size:
1687 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 223
Size: 3518
BsiEI CG_ry'CG
Cuts at: 104 1060 1106 2336 104
Size: 956 46 1230 1286
Fragments arranged by size:
1286 1230 956 46
BsiHKAI G_wGCw'C
Cuts at: 532 1310 1500 2360 532
Size: 778 190 860 1690
Fragments arranged by size:
1690 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 532 1143 1236 1310 1500 1562 2360 532
Size: 611 93 74 190 62 798 1690
Fragments arranged by size:
1690 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1950 1950
Size: 3518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 568 782 1084 1465 568
Size: 214 302 381 2621
Fragments arranged by size:
2621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1810 1864 3348 3513 50
Size: 1760 54 1484 165 55
Fragments arranged by size:
1760 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1430 1430
Size: 3518
BsrFI r'CCGG_y
Cuts at: 1516 1697 1516
Size: 181 3337
BssSI C'ACGA_G
Cuts at: 1789 2497 1789
Size: 708 2810
BstAPI GCAn_nnn'nTGC
Cuts at: 895 895
Size: 3518
BstBI TT'CG_AA
Cuts at: 1879 1879
Size: 3518
BstYI r'GATC_y
Cuts at: 1 543 1010 1033 1368 1614 1920 1932 2018
Size: 542 467 23 335 246 306 12 86
Cuts at: 2018 3070 1
Size: 1052 449
Fragments arranged by size:
1052 542 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 264 107
Size: 157 3361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1468 1649 1812 1468
Size: 181 163 3174
Fragments arranged by size:
3174 181 163
BtsI GCAGTG_nn'
Cuts at: 761 761
Size: 3518
DraI TTT'AAA
Cuts at: 517 517
Size: 3518
DrdI GACnn_nn'nnGTC
Cuts at: 1224 2568 1224
Size: 1344 2174
EaeI y'GGCC_r
Cuts at: 1103 1277 1668 1695 3040 1103
Size: 174 391 27 1345 1581
Fragments arranged by size:
1581 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1103 1103
Size: 3518
EarI CTCTTCn'nnn_
Cuts at: 1541 1751 1541
Size: 210 3308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1726 2468 2614 3328 1726
Size: 742 146 714 1916
Fragments arranged by size:
1916 742 714 146
EcoICRI GAG'CTC
Cuts at: 530 530
Size: 3518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 472 1343 1775 2128 2790 170
Size: 302 871 432 353 662 898
Fragments arranged by size:
898 871 662 432 353 302
EcoO109I rG'GnC_Cy
Cuts at: 308 657 308
Size: 349 3169
EcoRI G'AATT_C
Cuts at: 424 522 424
Size: 98 3420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1537 1537
Size: 3518
FspI TGC'GCA
Cuts at: 1299 1299
Size: 3518
HaeII r_GCGC'y
Cuts at: 952 1200 2430 3201 952
Size: 248 1230 771 1269
Fragments arranged by size:
1269 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1360 1390 1392 1422 1360
Size: 30 2 30 3456
Fragments arranged by size:
3456 30 30 2
HincII GTy'rAC
Cuts at: 557 557
Size: 3518
KasI G'GCGC_C
Cuts at: 948 1196 948
Size: 248 3270
KpnI G_GTAC'C
Cuts at: 538 538
Size: 3518
MluI A'CGCG_T
Cuts at: 2951 2951
Size: 3518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2277 2461 2277
Size: 184 3334
MscI TGG'CCA
Cuts at: 1279 1279
Size: 3518
MslI CAynn'nnrTG
Cuts at: 283 1507 1634 283
Size: 1224 127 2167
Fragments arranged by size:
2167 1224 127
MspA1I CmG'CkG
Cuts at: 327 475 629 943 1303 2087 2332 327
Size: 148 154 314 360 784 245 1513
Fragments arranged by size:
1513 784 360 314 245 154 148
NaeI GCC'GGC
Cuts at: 1699 1699
Size: 3518
NarI GG'CG_CC
Cuts at: 949 1197 949
Size: 248 3270
NcoI C'CATG_G
Cuts at: 107 107
Size: 3518
NgoMIV G'CCGG_C
Cuts at: 1697 1697
Size: 3518
NspI r_CATG'y
Cuts at: 571 1071 2674 571
Size: 500 1603 1415
Fragments arranged by size:
1603 1415 500
PciI A'CATG_T
Cuts at: 2670 2670
Size: 3518
PmeI GTTT'AAAC
Cuts at: 517 517
Size: 3518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1964 1996 1964
Size: 32 3486
PstI C_TGCA'G
Cuts at: 565 1250 565
Size: 685 2833
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3518
PvuII CAG'CTG
Cuts at: 475 943 1303 475
Size: 468 360 2690
Fragments arranged by size:
2690 468 360
RsrII CG'GwC_CG
Cuts at: 1713 1713
Size: 3518
SacI G_AGCT'C
Cuts at: 532 532
Size: 3518
SalI G'TCGA_C
Cuts at: 555 555
Size: 3518
SapI GCTCTTCn'nnn_
Cuts at: 1541 1751 1541
Size: 210 3308
SbfI CC_TGCA'GG
Cuts at: 565 565
Size: 3518
ScaI AGT'ACT
Cuts at: 3035 3035
Size: 3518
SfcI C'TryA_G
Cuts at: 33 561 1246 2214 2405 33
Size: 528 685 968 191 1146
Fragments arranged by size:
1146 968 685 528 191
SfoI GGC'GCC
Cuts at: 950 1198 950
Size: 248 3270
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
SmaI CCC'GGG
Cuts at: 540 540
Size: 3518
SmlI C'TyrA_G
Cuts at: 673 1588 2302 2564 673
Size: 915 714 262 1627
Fragments arranged by size:
1627 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3518
SphI G_CATG'C
Cuts at: 571 1071 571
Size: 500 3018
StyI C'CwwG_G
Cuts at: 107 652 1006 107
Size: 545 354 2619
Fragments arranged by size:
2619 545 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 1101 1234 1693 1902 334
Size: 767 133 459 209 1950
Fragments arranged by size:
1950 767 459 209 133
TatI w'GTAC_w
Cuts at: 3033 3033
Size: 3518
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3486
Tth111I GACn'n_nGTC
Cuts at: 1315 1315
Size: 3518
XbaI T'CTAG_A
Cuts at: 549 549
Size: 3518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 1013 465
Size: 548 2970
XmaI C'CCGG_G
Cuts at: 538 538
Size: 3518
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI