Restriction map for
pF1KB8433
(Circular) (Six-base) MAPSORT of: pF1KB8433.seq Check: 2877 from: 1 to: 3962
pF1KB8433 3962 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 321 321
Size: 3962
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3962
Acc65I G'GTAC_C
Cuts at: 503 978 503
Size: 475 3487
AclI AA'CG_TT
Cuts at: 3802 3802
Size: 3962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 406 769 1787 2219 2572 3234 217
Size: 189 363 1018 432 353 662 945
Fragments arranged by size:
1018 945 662 432 363 353 189
AfeI AGC'GCT
Cuts at: 946 946
Size: 3962
AflIII A'CryG_T
Cuts at: 557 1314 3114 3395 557
Size: 757 1800 281 1124
Fragments arranged by size:
1800 1124 757 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 3962
AlwNI CAG_nnn'CTG
Cuts at: 264 354 657 1267 2705 264
Size: 90 303 610 1438 1521
Fragments arranged by size:
1521 1438 610 303 90
ApaLI G'TGCA_C
Cuts at: 2800 2800
Size: 3962
ApoI r'AATT_y
Cuts at: 966 3315 3571 966
Size: 2349 256 1357
Fragments arranged by size:
2349 1357 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 449 982 449
Size: 533 3429
BamHI G'GATC_C
Cuts at: 987 987
Size: 3962
BanI G'GyrC_C
Cuts at: 503 978 1392 1640 1675 503
Size: 475 414 248 35 2790
Fragments arranged by size:
2790 475 414 248 35
BanII G_rGCy'C
Cuts at: 513 838 878 976 2006 513
Size: 325 40 98 1030 2469
Fragments arranged by size:
2469 1030 325 98 40
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3714
BbsI GAAGACnn'nnnn_
Cuts at: 433 433
Size: 3962
BciVI GTATCCnnnnn_n'
Cuts at: 1860 2916 3962 1860
Size: 1056 1046 1860
Fragments arranged by size:
1860 1056 1046
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3962
BglII A'GATC_T
Cuts at: 1477 3514 1477
Size: 2037 1925
BlpI GC'TnA_GC
Cuts at: 1074 1074
Size: 3962
Bme1580I G_kGCm'C
Cuts at: 664 1587 1680 2804 664
Size: 923 93 1124 1822
Fragments arranged by size:
1822 1124 923 93
BmrI ACTGGGnnnn_n'
Cuts at: 460 662 1174 1255 1590 3734 460
Size: 202 512 81 335 2144 688
Fragments arranged by size:
2144 688 512 335 202 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 3962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 298 331 850 244
Size: 54 33 519 3356
Fragments arranged by size:
3356 519 54 33
Bpu10I CC'TnA_GC
Cuts at: 308 308
Size: 3962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2017 2731 3029 1138
Size: 879 714 298 2071
Fragments arranged by size:
2071 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3666 3666
Size: 3962
BsaAI yAC'GTr
Cuts at: 1315 1315
Size: 3962
BsaBI GATnn'nnATC
Cuts at: 6 1476 3513 6
Size: 1470 2037 455
Fragments arranged by size:
2037 1470 455
BsaHI Gr'CG_yC
Cuts at: 1393 1641 2343 3839 1393
Size: 248 702 1496 1516
Fragments arranged by size:
1516 1496 702 248
BsaWI w'CCGG_w
Cuts at: 725 1151 1672 2350 2761 2908 3182 725
Size: 426 521 678 411 147 274 1505
Fragments arranged by size:
1505 678 521 426 411 274 147
BseYI C'CCAG_C
Cuts at: 657 918 1387 2145 2810 3218 657
Size: 261 469 758 665 408 1401
Fragments arranged by size:
1401 758 665 469 408 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 670 778 340
Size: 330 108 3524
Fragments arranged by size:
3524 330 108
BsiEI CG_ry'CG
Cuts at: 104 818 1504 1550 2780 104
Size: 714 686 46 1230 1286
Fragments arranged by size:
1286 1230 714 686 46
BsiHKAI G_wGCw'C
Cuts at: 338 838 878 976 1754 1944 2804 338
Size: 500 40 98 778 190 860 1496
Fragments arranged by size:
1496 860 778 500 190 98 40
Bsp1286I G_dGCh'C
Cuts at: 338 513 664 838 878 976 1587 1680 1754
Size: 175 151 174 40 98 611 93 74
Cuts at: 1754 1944 2006 2804 338
Size: 190 62 798 1496
Fragments arranged by size:
1496 798 611 190 175 174 151 98
93 74 62 40
BspEI T'CCGG_A
Cuts at: 725 725
Size: 3962
BspHI T'CATG_A
Cuts at: 404 2394 404
Size: 1990 1972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 321 1012 1226 1528 1909 321
Size: 691 214 302 381 2374
Fragments arranged by size:
2374 691 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2254 2308 3792 3957 50
Size: 2204 54 1484 165 55
Fragments arranged by size:
2204 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 1874
Size: 3962
BsrFI r'CCGG_y
Cuts at: 1960 2141 1960
Size: 181 3781
BssSI C'ACGA_G
Cuts at: 2233 2941 2233
Size: 708 3254
BstAPI GCAn_nnn'nTGC
Cuts at: 1339 1339
Size: 3962
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstXI CCAn_nnnn'nTGG
Cuts at: 276 276
Size: 3962
BstYI r'GATC_y
Cuts at: 1 626 987 1454 1477 1812 2058 2364 2376
Size: 625 361 467 23 335 246 306 12
Cuts at: 2376 2462 3514 1
Size: 86 1052 449
Fragments arranged by size:
1052 625 467 449 361 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 763 107
Size: 656 3306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1912 2093 2256 1912
Size: 181 163 3618
Fragments arranged by size:
3618 181 163
BtsI GCAGTG_nn'
Cuts at: 1205 1205
Size: 3962
DraI TTT'AAA
Cuts at: 961 961
Size: 3962
DrdI GACnn_nn'nnGTC
Cuts at: 1668 3012 1668
Size: 1344 2618
EaeI y'GGCC_r
Cuts at: 266 581 682 711 815 1547 1721 2112 2139
Size: 315 101 29 104 732 174 391 27
Cuts at: 2139 3484 266
Size: 1345 744
Fragments arranged by size:
1345 744 732 391 315 174 104 101
29 27
EagI C'GGCC_G
Cuts at: 815 1547 815
Size: 732 3230
EarI CTCTTCn'nnn_
Cuts at: 611 1985 2195 611
Size: 1374 210 2378
Fragments arranged by size:
2378 1374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 2170 2912 3058 3772 157
Size: 2013 742 146 714 347
Fragments arranged by size:
2013 742 714 347 146
EcoICRI GAG'CTC
Cuts at: 836 876 974 836
Size: 40 98 3824
Fragments arranged by size:
3824 98 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 244 298 331 406 769 850 1787 2219
Size: 27 54 33 75 363 81 937 432
Cuts at: 2219 2572 3234 217
Size: 353 662 945
Fragments arranged by size:
945 937 662 432 363 353 81 75
54 33 27
EcoO109I rG'GnC_Cy
Cuts at: 261 342 381 1101 261
Size: 81 39 720 3122
Fragments arranged by size:
3122 720 81 39
EcoRI G'AATT_C
Cuts at: 966 966
Size: 3962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 1743 1743
Size: 3962
HaeII r_GCGC'y
Cuts at: 948 1396 1644 2874 3645 948
Size: 448 248 1230 771 1265
Fragments arranged by size:
1265 1230 771 448 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1804 1834 1836 1866 1804
Size: 30 2 30 3900
Fragments arranged by size:
3900 30 30 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3962
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3714
KpnI G_GTAC'C
Cuts at: 507 982 507
Size: 475 3487
MluI A'CGCG_T
Cuts at: 3395 3395
Size: 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 419 752 2721 2905 419
Size: 333 1969 184 1476
Fragments arranged by size:
1969 1476 333 184
MscI TGG'CCA
Cuts at: 268 1723 268
Size: 1455 2507
MslI CAynn'nnrTG
Cuts at: 556 1951 2078 556
Size: 1395 127 2440
Fragments arranged by size:
2440 1395 127
MspA1I CmG'CkG
Cuts at: 433 654 1073 1387 1747 2531 2776 433
Size: 221 419 314 360 784 245 1619
Fragments arranged by size:
1619 784 419 360 314 245 221
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3714
NcoI C'CATG_G
Cuts at: 107 763 107
Size: 656 3306
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NspI r_CATG'y
Cuts at: 561 1015 1515 3118 561
Size: 454 500 1603 1405
Fragments arranged by size:
1603 1405 500 454
PciI A'CATG_T
Cuts at: 557 3114 557
Size: 2557 1405
PfoI T'CCnGG_A
Cuts at: 275 275
Size: 3962
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2408 2440 2408
Size: 32 3930
PpuMI rG'GwC_Cy
Cuts at: 261 381 261
Size: 120 3842
PstI C_TGCA'G
Cuts at: 332 1009 1694 332
Size: 677 685 2600
Fragments arranged by size:
2600 685 677
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 654 1387 1747 654
Size: 733 360 2869
Fragments arranged by size:
2869 733 360
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 838 878 976 838
Size: 40 98 3824
Fragments arranged by size:
3824 98 40
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 3479 3479
Size: 3962
SfcI C'TryA_G
Cuts at: 33 328 1005 1690 2658 2849 33
Size: 295 677 685 968 191 1146
Fragments arranged by size:
1146 968 685 677 295 191
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3714
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SmaI CCC'GGG
Cuts at: 451 984 451
Size: 533 3429
SmlI C'TyrA_G
Cuts at: 1117 2032 2746 3008 1117
Size: 915 714 262 2071
Fragments arranged by size:
2071 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3462
SrfI GCCC'GGGC
Cuts at: 451 451
Size: 3962
StuI AGG'CCT
Cuts at: 603 603
Size: 3962
StyI C'CwwG_G
Cuts at: 107 485 763 1096 1450 107
Size: 378 278 333 354 2619
Fragments arranged by size:
2619 378 354 333 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 1545 1678 2137 2346 424
Size: 1121 133 459 209 2040
Fragments arranged by size:
2040 1121 459 209 133
TatI w'GTAC_w
Cuts at: 716 3477 716
Size: 2761 1201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 3930
Tth111I GACn'n_nGTC
Cuts at: 1759 1759
Size: 3962
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 702 1457 702
Size: 755 3207
XmaI C'CCGG_G
Cuts at: 449 982 449
Size: 533 3429
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI SwaI XhoI XmnI ZraI