Restriction map for
pF1KB8425
(Circular) (Six-base) MAPSORT of: pF1KB8425.seq Check: 6043 from: 1 to: 5177
pF1KB8425 5177 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 2215 2215
Size: 5177
Acc65I G'GTAC_C
Cuts at: 2193 2193
Size: 5177
AclI AA'CG_TT
Cuts at: 5017 5017
Size: 5177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 641 825 1246 1793 2144 3002 3434 3787
Size: 438 184 421 547 351 858 432 353
Cuts at: 3787 4449 203
Size: 662 931
Fragments arranged by size:
931 858 662 547 438 432 421 353
351 184
AfeI AGC'GCT
Cuts at: 812 812
Size: 5177
AflII C'TTAA_G
Cuts at: 1885 1885
Size: 5177
AflIII A'CryG_T
Cuts at: 2529 4329 4610 2529
Size: 1800 281 3096
Fragments arranged by size:
3096 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 287 287
Size: 5177
AlwNI CAG_nnn'CTG
Cuts at: 369 1021 2482 3920 369
Size: 652 1461 1438 1626
Fragments arranged by size:
1626 1461 1438 652
ApaLI G'TGCA_C
Cuts at: 1623 4015 1623
Size: 2392 2785
ApoI r'AATT_y
Cuts at: 211 637 867 913 922 1444 1918 2089 2181
Size: 426 230 46 9 522 474 171 92
Cuts at: 2181 4530 4786 211
Size: 2349 256 602
Fragments arranged by size:
2349 602 522 474 426 256 230 171
92 46 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 5177
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5177
AvaI C'yCGr_G
Cuts at: 671 1957 2197 671
Size: 1286 240 3651
Fragments arranged by size:
3651 1286 240
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1673 1706 1673
Size: 33 5144
BamHI G'GATC_C
Cuts at: 2202 2202
Size: 5177
BanI G'GyrC_C
Cuts at: 978 2193 2607 2855 2890 978
Size: 1215 414 248 35 3265
Fragments arranged by size:
3265 1215 414 248 35
BanII G_rGCy'C
Cuts at: 1670 2191 3221 1670
Size: 521 1030 3626
Fragments arranged by size:
3626 1030 521
BbeI G_GCGC'C
Cuts at: 2611 2859 2611
Size: 248 4929
BbsI GAAGACnn'nnnn_
Cuts at: 254 286 386 254
Size: 32 100 5045
Fragments arranged by size:
5045 100 32
BbvCI CC'TCA_GC
Cuts at: 1731 1731
Size: 5177
BciVI GTATCCnnnnn_n'
Cuts at: 3075 4131 5177 3075
Size: 1056 1046 3075
Fragments arranged by size:
3075 1056 1046
BclI T'GATC_A
Cuts at: 2697 2697
Size: 5177
BglII A'GATC_T
Cuts at: 1364 2692 4729 1364
Size: 1328 2037 1812
Fragments arranged by size:
2037 1812 1328
BlpI GC'TnA_GC
Cuts at: 2289 2289
Size: 5177
Bme1580I G_kGCm'C
Cuts at: 1627 2802 2895 4019 1627
Size: 1175 93 1124 2785
Fragments arranged by size:
2785 1175 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1614 2062 2163 2178 2389 2470 2805 4949 1614
Size: 448 101 15 211 81 335 2144 1842
Fragments arranged by size:
2144 1842 448 335 211 101 81 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3544 3544
Size: 5177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 735 1120 1560 617
Size: 118 385 440 4234
Fragments arranged by size:
4234 440 385 118
Bpu10I CC'TnA_GC
Cuts at: 1731 1731
Size: 5177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2353 3232 3946 4244 2353
Size: 879 714 298 3286
Fragments arranged by size:
3286 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 593 1323 4881 593
Size: 730 3558 889
Fragments arranged by size:
3558 889 730
BsaAI yAC'GTr
Cuts at: 1200 2530 1200
Size: 1330 3847
BsaBI GATnn'nnATC
Cuts at: 6 312 2691 4728 6
Size: 306 2379 2037 455
Fragments arranged by size:
2379 2037 455 306
BsaHI Gr'CG_yC
Cuts at: 2608 2856 3558 5054 2608
Size: 248 702 1496 2731
Fragments arranged by size:
2731 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2366 2887 3565 3976 4123 4397 2366
Size: 521 678 411 147 274 3146
Fragments arranged by size:
3146 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 594 916 1364 1540 1567 594
Size: 322 448 176 27 4204
Fragments arranged by size:
4204 448 322 176 27
BseYI C'CCAG_C
Cuts at: 412 603 1254 1669 2129 2602 3360 4025 4433
Size: 191 651 415 460 473 758 665 408
Cuts at: 4433 412
Size: 1156
Fragments arranged by size:
1156 758 665 651 473 460 415 408
191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 801
Size: 5177
BsiEI CG_ry'CG
Cuts at: 104 2719 2765 3995 104
Size: 2615 46 1230 1286
Fragments arranged by size:
2615 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1208 1627 2191 2969 3159 4019 1208
Size: 419 564 778 190 860 2366
Fragments arranged by size:
2366 860 778 564 419 190
BsmI GAATG_Cn'
Cuts at: 338 338
Size: 5177
Bsp1286I G_dGCh'C
Cuts at: 1208 1627 1670 2191 2802 2895 2969 3159 3221
Size: 419 43 521 611 93 74 190 62
Cuts at: 3221 4019 1208
Size: 798 2366
Fragments arranged by size:
2366 798 611 521 419 190 93 74
62 43
BspHI T'CATG_A
Cuts at: 450 831 1152 3609 450
Size: 381 321 2457 2018
Fragments arranged by size:
2457 2018 381 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2227 2441 2743 3124 2227
Size: 214 302 381 4280
Fragments arranged by size:
4280 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3469 3523 5007 5172 50
Size: 3419 54 1484 165 55
Fragments arranged by size:
3419 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3089 3089
Size: 5177
BsrFI r'CCGG_y
Cuts at: 3175 3356 3175
Size: 181 4996
BsrGI T'GTAC_A
Cuts at: 1821 1821
Size: 5177
BssSI C'ACGA_G
Cuts at: 3448 4156 3448
Size: 708 4469
BstAPI GCAn_nnn'nTGC
Cuts at: 2554 2554
Size: 5177
BstBI TT'CG_AA
Cuts at: 3538 3538
Size: 5177
BstYI r'GATC_y
Cuts at: 1 416 1364 1953 2202 2669 2692 3027 3273
Size: 415 948 589 249 467 23 335 246
Cuts at: 3273 3579 3591 3677 4729 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 948 589 467 449 415 335 306
249 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1550 1550
Size: 5177
BtgI C'CryG_G
Cuts at: 107 107
Size: 5177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3127 3308 3471 3127
Size: 181 163 4833
Fragments arranged by size:
4833 181 163
BtsI GCAGTG_nn'
Cuts at: 1316 1376 1709 2420 1316
Size: 60 333 711 4073
Fragments arranged by size:
4073 711 333 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1232 1267 1232
Size: 35 5142
DraI TTT'AAA
Cuts at: 131 921 2176 131
Size: 790 1255 3132
Fragments arranged by size:
3132 1255 790
DrdI GACnn_nn'nnGTC
Cuts at: 2883 4227 2883
Size: 1344 3833
EaeI y'GGCC_r
Cuts at: 805 2762 2936 3327 3354 4699 805
Size: 1957 174 391 27 1345 1283
Fragments arranged by size:
1957 1345 1283 391 174 27
EagI C'GGCC_G
Cuts at: 2762 2762
Size: 5177
EarI CTCTTCn'nnn_
Cuts at: 617 886 893 989 1151 1637 1769 3200 3410
Size: 269 7 96 162 486 132 1431 210
Cuts at: 3410 617
Size: 2384
Fragments arranged by size:
2384 1431 486 269 210 162 132 96
7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3385 4127 4273 4987 3385
Size: 742 146 714 3575
Fragments arranged by size:
3575 742 714 146
EcoICRI GAG'CTC
Cuts at: 2189 2189
Size: 5177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 617 641 735 825 1120 1246 1560 1793
Size: 414 24 94 90 295 126 314 233
Cuts at: 1793 2144 3002 3434 3787 4449 203
Size: 351 858 432 353 662 931
Fragments arranged by size:
931 858 662 432 414 353 351 314
295 233 126 94 90 24
EcoO109I rG'GnC_Cy
Cuts at: 1880 2316 1880
Size: 436 4741
EcoRI G'AATT_C
Cuts at: 637 913 1918 2181 637
Size: 276 1005 263 3633
Fragments arranged by size:
3633 1005 276 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 5177
FspI TGC'GCA
Cuts at: 1070 2958 1070
Size: 1888 3289
HaeII r_GCGC'y
Cuts at: 814 2611 2859 4089 4860 814
Size: 1797 248 1230 771 1131
Fragments arranged by size:
1797 1230 1131 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 273 305 592 624 733 765 1600 1632 1691
Size: 32 287 32 109 32 835 32 59
Cuts at: 1691 1723 2047 2079 3019 3049 3051 3081 273
Size: 32 324 32 940 30 2 30 2369
Fragments arranged by size:
2369 940 835 324 287 109 59 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1575 2216 1575
Size: 641 4536
HindIII A'AGCT_T
Cuts at: 1797 1797
Size: 5177
KasI G'GCGC_C
Cuts at: 2607 2855 2607
Size: 248 4929
KpnI G_GTAC'C
Cuts at: 2197 2197
Size: 5177
MluI A'CGCG_T
Cuts at: 4610 4610
Size: 5177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3936 4120 3936
Size: 184 4993
MscI TGG'CCA
Cuts at: 2938 2938
Size: 5177
MslI CAynn'nnrTG
Cuts at: 476 665 3166 3293 476
Size: 189 2501 127 2360
Fragments arranged by size:
2501 2360 189 127
MspA1I CmG'CkG
Cuts at: 193 739 944 1224 1735 2288 2602 2962 3746
Size: 546 205 280 511 553 314 360 784
Cuts at: 3746 3991 193
Size: 245 1379
Fragments arranged by size:
1379 784 553 546 511 360 314 280
245 205
NaeI GCC'GGC
Cuts at: 3358 3358
Size: 5177
NarI GG'CG_CC
Cuts at: 2608 2856 2608
Size: 248 4929
NcoI C'CATG_G
Cuts at: 107 107
Size: 5177
NgoMIV G'CCGG_C
Cuts at: 3356 3356
Size: 5177
NspI r_CATG'y
Cuts at: 670 1165 2230 2730 4333 670
Size: 495 1065 500 1603 1514
Fragments arranged by size:
1603 1514 1065 500 495
PciI A'CATG_T
Cuts at: 4329 4329
Size: 5177
PflMI CCAn_nnn'nTGG
Cuts at: 1702 1702
Size: 5177
PmeI GTTT'AAAC
Cuts at: 2176 2176
Size: 5177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 5145
PstI C_TGCA'G
Cuts at: 402 2100 2224 2909 402
Size: 1698 124 685 2670
Fragments arranged by size:
2670 1698 685 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5177
PvuII CAG'CTG
Cuts at: 944 1224 2602 2962 944
Size: 280 1378 360 3159
Fragments arranged by size:
3159 1378 360 280
RsrII CG'GwC_CG
Cuts at: 3372 3372
Size: 5177
SacI G_AGCT'C
Cuts at: 2191 2191
Size: 5177
SalI G'TCGA_C
Cuts at: 2214 2214
Size: 5177
SapI GCTCTTCn'nnn_
Cuts at: 1151 3200 3410 1151
Size: 2049 210 2918
Fragments arranged by size:
2918 2049 210
SbfI CC_TGCA'GG
Cuts at: 2224 2224
Size: 5177
ScaI AGT'ACT
Cuts at: 4694 4694
Size: 5177
SfcI C'TryA_G
Cuts at: 33 398 2096 2220 2905 3873 4064 33
Size: 365 1698 124 685 968 191 1146
Fragments arranged by size:
1698 1146 968 685 365 191 124
SfoI GGC'GCC
Cuts at: 2609 2857 2609
Size: 248 4929
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5177
SmaI CCC'GGG
Cuts at: 673 2199 673
Size: 1526 3651
SmlI C'TyrA_G
Cuts at: 1885 2332 3247 3961 4223 1885
Size: 447 915 714 262 2839
Fragments arranged by size:
2839 915 714 447 262
SpeI A'CTAG_T
Cuts at: 67 1270 67
Size: 1203 3974
SphI G_CATG'C
Cuts at: 2230 2730 2230
Size: 500 4677
StuI AGG'CCT
Cuts at: 1195 1195
Size: 5177
StyI C'CwwG_G
Cuts at: 107 1631 1786 2311 2665 107
Size: 1524 155 525 354 2619
Fragments arranged by size:
2619 1524 525 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2760 2893 3352 3561 2760
Size: 133 459 209 4376
Fragments arranged by size:
4376 459 209 133
TatI w'GTAC_w
Cuts at: 195 319 360 1821 4692 195
Size: 124 41 1461 2871 680
Fragments arranged by size:
2871 1461 680 124 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 1106 1138 1322 1354 4694 4726 683
Size: 32 391 32 184 32 3340 32 1134
Fragments arranged by size:
3340 1134 391 184 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 937 2974 937
Size: 2037 3140
XbaI T'CTAG_A
Cuts at: 1413 2208 1413
Size: 795 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2672 2672
Size: 5177
XmaI C'CCGG_G
Cuts at: 671 2197 671
Size: 1526 3651
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AgeI AleI AlfI AloI ApaI AscI
AvrII BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BspEI BssHII BstEII BstXI BstZ17I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI