Restriction map for
pF1KB8417
(Circular) (Six-base) MAPSORT of: pF1KB8417.seq Check: 7986 from: 1 to: 5468
pF1KB8417 5468 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 2506 2506
Size: 5468
Acc65I G'GTAC_C
Cuts at: 2484 2484
Size: 5468
AclI AA'CG_TT
Cuts at: 5308 5308
Size: 5468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 1190 1691 3293 3725 4078 4740 831
Size: 359 501 1602 432 353 662 1559
Fragments arranged by size:
1602 1559 662 501 432 359 353
AflII C'TTAA_G
Cuts at: 1975 1975
Size: 5468
AflIII A'CryG_T
Cuts at: 354 1535 2820 4620 4901 354
Size: 1181 1285 1800 281 921
Fragments arranged by size:
1800 1285 1181 921 281
AlwNI CAG_nnn'CTG
Cuts at: 1306 2773 4211 1306
Size: 1467 1438 2563
Fragments arranged by size:
2563 1467 1438
ApaLI G'TGCA_C
Cuts at: 4306 4306
Size: 5468
ApoI r'AATT_y
Cuts at: 900 2472 4821 5077 900
Size: 1572 2349 256 1291
Fragments arranged by size:
2349 1572 1291 256
AseI AT'TA_AT
Cuts at: 20 1400 20
Size: 1380 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
AvaI C'yCGr_G
Cuts at: 2488 2488
Size: 5468
BamHI G'GATC_C
Cuts at: 2493 2493
Size: 5468
BanI G'GyrC_C
Cuts at: 111 2484 2898 3146 3181 111
Size: 2373 414 248 35 2398
Fragments arranged by size:
2398 2373 414 248 35
BanII G_rGCy'C
Cuts at: 239 1899 2482 3512 239
Size: 1660 583 1030 2195
Fragments arranged by size:
2195 1660 1030 583
BbeI G_GCGC'C
Cuts at: 115 2902 3150 115
Size: 2787 248 2433
Fragments arranged by size:
2787 2433 248
BbsI GAAGACnn'nnnn_
Cuts at: 837 964 1337 837
Size: 127 373 4968
Fragments arranged by size:
4968 373 127
BbvCI CC'TCA_GC
Cuts at: 1991 1991
Size: 5468
BciVI GTATCCnnnnn_n'
Cuts at: 951 1129 1594 3366 4422 5468 951
Size: 178 465 1772 1056 1046 951
Fragments arranged by size:
1772 1056 1046 951 465 178
BclI T'GATC_A
Cuts at: 267 2988 267
Size: 2721 2747
BglI GCCn_nnn'nGGC
Cuts at: 627 627
Size: 5468
BglII A'GATC_T
Cuts at: 852 2084 2983 5020 852
Size: 1232 899 2037 1300
Fragments arranged by size:
2037 1300 1232 899
BlpI GC'TnA_GC
Cuts at: 2580 2580
Size: 5468
Bme1580I G_kGCm'C
Cuts at: 2064 3093 3186 4310 2064
Size: 1029 93 1124 3222
Fragments arranged by size:
3222 1124 1029 93
BmrI ACTGGGnnnn_n'
Cuts at: 331 1645 2680 2761 3096 5240 331
Size: 1314 1035 81 335 2144 559
Fragments arranged by size:
2144 1314 1035 559 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 177 3835 177
Size: 3658 1810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1095
Size: 5468
Bpu10I CC'TnA_GC
Cuts at: 328 1991 328
Size: 1663 3805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1652 2644 3523 4237 4535 208
Size: 1444 992 879 714 298 1141
Fragments arranged by size:
1444 1141 992 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5172 5172
Size: 5468
BsaAI yAC'GTr
Cuts at: 2821 2821
Size: 5468
BsaBI GATnn'nnATC
Cuts at: 6 380 2982 5019 6
Size: 374 2602 2037 455
Fragments arranged by size:
2602 2037 455 374
BsaHI Gr'CG_yC
Cuts at: 112 1229 2899 3147 3849 5345 112
Size: 1117 1670 248 702 1496 235
Fragments arranged by size:
1670 1496 1117 702 248 235
BsaWI w'CCGG_w
Cuts at: 485 1922 2657 3178 3856 4267 4414 4688 485
Size: 1437 735 521 678 411 147 274 1265
Fragments arranged by size:
1437 1265 735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2132 2162 2132
Size: 30 5438
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 680 680
Size: 5468
BseYI C'CCAG_C
Cuts at: 2063 2893 3651 4316 4724 2063
Size: 830 758 665 408 2807
Fragments arranged by size:
2807 830 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1367 1367
Size: 5468
BsiEI CG_ry'CG
Cuts at: 104 3010 3056 4286 104
Size: 2906 46 1230 1286
Fragments arranged by size:
2906 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2482 3260 3450 4310 2482
Size: 778 190 860 3640
Fragments arranged by size:
3640 860 778 190
BsmI GAATG_Cn'
Cuts at: 1357 1357
Size: 5468
Bsp1286I G_dGCh'C
Cuts at: 239 1899 2064 2482 3093 3186 3260 3450 3512
Size: 1660 165 418 611 93 74 190 62
Cuts at: 3512 4310 239
Size: 798 1397
Fragments arranged by size:
1660 1397 798 611 418 190 165 93
74 62
BspHI T'CATG_A
Cuts at: 3900 3900
Size: 5468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2518 2732 3034 3415 2518
Size: 214 302 381 4571
Fragments arranged by size:
4571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3760 3814 5298 5463 50
Size: 3710 54 1484 165 55
Fragments arranged by size:
3710 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 654 3380 654
Size: 2726 2742
BsrFI r'CCGG_y
Cuts at: 3466 3647 3466
Size: 181 5287
BsrGI T'GTAC_A
Cuts at: 2076 2076
Size: 5468
BssSI C'ACGA_G
Cuts at: 2080 3739 4447 2080
Size: 1659 708 3101
Fragments arranged by size:
3101 1659 708
BstAPI GCAn_nnn'nTGC
Cuts at: 198 1702 2845 198
Size: 1504 1143 2821
Fragments arranged by size:
2821 1504 1143
BstBI TT'CG_AA
Cuts at: 3829 3829
Size: 5468
BstXI CCAn_nnnn'nTGG
Cuts at: 1612 1612
Size: 5468
BstYI r'GATC_y
Cuts at: 1 852 2084 2493 2960 2983 3318 3564 3870
Size: 851 1232 409 467 23 335 246 306
Cuts at: 3870 3882 3968 5020 1
Size: 12 86 1052 449
Fragments arranged by size:
1232 1052 851 467 449 409 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 810 107
Size: 703 4765
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3418 3599 3762 3418
Size: 181 163 5124
Fragments arranged by size:
5124 181 163
BtsI GCAGTG_nn'
Cuts at: 2711 2711
Size: 5468
DraI TTT'AAA
Cuts at: 2294 2441 2467 2294
Size: 147 26 5295
Fragments arranged by size:
5295 147 26
DrdI GACnn_nn'nnGTC
Cuts at: 3174 4518 3174
Size: 1344 4124
EaeI y'GGCC_r
Cuts at: 167 3053 3227 3618 3645 4990 167
Size: 2886 174 391 27 1345 645
Fragments arranged by size:
2886 1345 645 391 174 27
EagI C'GGCC_G
Cuts at: 3053 3053
Size: 5468
EarI CTCTTCn'nnn_
Cuts at: 244 1019 3491 3701 244
Size: 775 2472 210 2011
Fragments arranged by size:
2472 2011 775 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3676 4418 4564 5278 3676
Size: 742 146 714 3866
Fragments arranged by size:
3866 742 714 146
EcoICRI GAG'CTC
Cuts at: 2480 2480
Size: 5468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 1095 1190 1691 3293 3725 4078 4740 831
Size: 264 95 501 1602 432 353 662 1559
Fragments arranged by size:
1602 1559 662 501 432 353 264 95
EcoNI CCTnn'n_nnAGG
Cuts at: 1130 1130
Size: 5468
EcoO109I rG'GnC_Cy
Cuts at: 1123 1987 2607 1123
Size: 864 620 3984
Fragments arranged by size:
3984 864 620
EcoRI G'AATT_C
Cuts at: 900 2472 900
Size: 1572 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 534 1195 3487 534
Size: 661 2292 2515
Fragments arranged by size:
2515 2292 661
FspI TGC'GCA
Cuts at: 3249 3249
Size: 5468
HaeII r_GCGC'y
Cuts at: 115 2902 3150 4380 5151 115
Size: 2787 248 1230 771 432
Fragments arranged by size:
2787 1230 771 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1044 1076 1093 1125 3310 3340 3342 3372 1044
Size: 32 17 32 2185 30 2 30 3140
Fragments arranged by size:
3140 2185 32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 2138 2507 2138
Size: 369 5099
HpaI GTT'AAC
Cuts at: 2138 2138
Size: 5468
KasI G'GCGC_C
Cuts at: 111 2898 3146 111
Size: 2787 248 2433
Fragments arranged by size:
2787 2433 248
KpnI G_GTAC'C
Cuts at: 2488 2488
Size: 5468
MfeI C'AATT_G
Cuts at: 905 2106 905
Size: 1201 4267
MluI A'CGCG_T
Cuts at: 4901 4901
Size: 5468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1683 2303 4227 4411 1683
Size: 620 1924 184 2740
Fragments arranged by size:
2740 1924 620 184
MscI TGG'CCA
Cuts at: 169 3229 169
Size: 3060 2408
MslI CAynn'nnrTG
Cuts at: 3457 3584 3457
Size: 127 5341
MspA1I CmG'CkG
Cuts at: 2364 2401 2579 2893 3253 4037 4282 2364
Size: 37 178 314 360 784 245 3550
Fragments arranged by size:
3550 784 360 314 245 178 37
NaeI GCC'GGC
Cuts at: 3649 3649
Size: 5468
NarI GG'CG_CC
Cuts at: 112 2899 3147 112
Size: 2787 248 2433
Fragments arranged by size:
2787 2433 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5468
NdeI CA'TA_TG
Cuts at: 264 264
Size: 5468
NgoMIV G'CCGG_C
Cuts at: 3647 3647
Size: 5468
NspI r_CATG'y
Cuts at: 358 865 1539 2521 3021 4624 358
Size: 507 674 982 500 1603 1202
Fragments arranged by size:
1603 1202 982 674 507 500
PciI A'CATG_T
Cuts at: 354 1535 4620 354
Size: 1181 3085 1202
Fragments arranged by size:
3085 1202 1181
PmeI GTTT'AAAC
Cuts at: 2467 2467
Size: 5468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 5436
PpuMI rG'GwC_Cy
Cuts at: 1123 1987 1123
Size: 864 4604
PsiI TTA'TAA
Cuts at: 1205 1768 1841 2372 1205
Size: 563 73 531 4301
Fragments arranged by size:
4301 563 531 73
PstI C_TGCA'G
Cuts at: 2515 3200 2515
Size: 685 4783
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5468
PvuII CAG'CTG
Cuts at: 2401 2893 3253 2401
Size: 492 360 4616
Fragments arranged by size:
4616 492 360
RsrII CG'GwC_CG
Cuts at: 3663 3663
Size: 5468
SacI G_AGCT'C
Cuts at: 2482 2482
Size: 5468
SalI G'TCGA_C
Cuts at: 2505 2505
Size: 5468
SapI GCTCTTCn'nnn_
Cuts at: 244 3491 3701 244
Size: 3247 210 2011
Fragments arranged by size:
3247 2011 210
SbfI CC_TGCA'GG
Cuts at: 2515 2515
Size: 5468
ScaI AGT'ACT
Cuts at: 197 4985 197
Size: 4788 680
SfcI C'TryA_G
Cuts at: 33 131 1499 1673 2511 3196 4164 4355 33
Size: 98 1368 174 838 685 968 191 1146
Fragments arranged by size:
1368 1146 968 838 685 191 174 98
SfoI GGC'GCC
Cuts at: 113 2900 3148 113
Size: 2787 248 2433
Fragments arranged by size:
2787 2433 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
SmaI CCC'GGG
Cuts at: 2490 2490
Size: 5468
SmlI C'TyrA_G
Cuts at: 223 1667 1975 2623 3538 4252 4514 223
Size: 1444 308 648 915 714 262 1177
Fragments arranged by size:
1444 1177 915 714 648 308 262
SpeI A'CTAG_T
Cuts at: 67 1246 2277 67
Size: 1179 1031 3258
Fragments arranged by size:
3258 1179 1031
SphI G_CATG'C
Cuts at: 865 2521 3021 865
Size: 1656 500 3312
Fragments arranged by size:
3312 1656 500
SspI AAT'ATT
Cuts at: 2207 2207
Size: 5468
StyI C'CwwG_G
Cuts at: 107 231 427 2166 2602 2956 107
Size: 124 196 1739 436 354 2619
Fragments arranged by size:
2619 1739 436 354 196 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 696 1490 2395 3051 3184 3643 3852 696
Size: 794 905 656 133 459 209 2312
Fragments arranged by size:
2312 905 794 656 459 209 133
TatI w'GTAC_w
Cuts at: 195 697 747 1542 1935 2076 4983 195
Size: 502 50 795 393 141 2907 680
Fragments arranged by size:
2907 795 680 502 393 141 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 4985 5017 2132
Size: 32 2821 32 2583
Fragments arranged by size:
2821 2583 32 32
Tth111I GACn'n_nGTC
Cuts at: 3265 3265
Size: 5468
XbaI T'CTAG_A
Cuts at: 2499 2499
Size: 5468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2963 2963
Size: 5468
XmaI C'CCGG_G
Cuts at: 2488 2488
Size: 5468
XmnI GAAnn'nnTTC
Cuts at: 1013 1175 1013
Size: 162 5306
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI