Restriction map for
pF1KB8416
(Circular) (Six-base) MAPSORT of: pF1KB8416.seq Check: 6066 from: 1 to: 6269
pF1KB8416 6269 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 243 1838 3307 243
Size: 1595 1469 3205
Fragments arranged by size:
3205 1595 1469
Acc65I G'GTAC_C
Cuts at: 3285 3285
Size: 6269
AclI AA'CG_TT
Cuts at: 6109 6109
Size: 6269
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1928 2239 2365 2617 2945 3231 4094 4526
Size: 1443 311 126 252 328 286 863 432
Cuts at: 4526 4879 5541 485
Size: 353 662 1213
Fragments arranged by size:
1443 1213 863 662 432 353 328 311
286 252 126
AflII C'TTAA_G
Cuts at: 303 1443 303
Size: 1140 5129
AflIII A'CryG_T
Cuts at: 3621 5421 5702 3621
Size: 1800 281 4188
Fragments arranged by size:
4188 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 214 1479 1600 2812 3574 5012 214
Size: 1265 121 1212 762 1438 1471
Fragments arranged by size:
1471 1438 1265 1212 762 121
ApaLI G'TGCA_C
Cuts at: 5107 5107
Size: 6269
ApoI r'AATT_y
Cuts at: 189 677 712 752 1333 1411 1485 1539 1704
Size: 488 35 40 581 78 74 54 165
Cuts at: 1704 2080 2344 2488 3273 5622 5878 189
Size: 376 264 144 785 2349 256 580
Fragments arranged by size:
2349 785 581 580 488 376 264 256
165 144 78 74 54 40 35
AseI AT'TA_AT
Cuts at: 20 857 20
Size: 837 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
AvaI C'yCGr_G
Cuts at: 3289 3289
Size: 6269
BamHI G'GATC_C
Cuts at: 115 1953 3294 115
Size: 1838 1341 3090
Fragments arranged by size:
3090 1838 1341
BanI G'GyrC_C
Cuts at: 1068 3285 3699 3947 3982 1068
Size: 2217 414 248 35 3355
Fragments arranged by size:
3355 2217 414 248 35
BanII G_rGCy'C
Cuts at: 3283 4313 3283
Size: 1030 5239
BbeI G_GCGC'C
Cuts at: 3703 3951 3703
Size: 248 6021
BbsI GAAGACnn'nnnn_
Cuts at: 2187 2187
Size: 6269
BciVI GTATCCnnnnn_n'
Cuts at: 4167 5223 6269 4167
Size: 1056 1046 4167
Fragments arranged by size:
4167 1056 1046
BclI T'GATC_A
Cuts at: 2667 3789 2667
Size: 1122 5147
BfrBI ATG'CAT
Cuts at: 232 3135 232
Size: 2903 3366
BglII A'GATC_T
Cuts at: 546 3784 5821 546
Size: 3238 2037 994
Fragments arranged by size:
3238 2037 994
BlpI GC'TnA_GC
Cuts at: 1851 3381 1851
Size: 1530 4739
Bme1580I G_kGCm'C
Cuts at: 643 1894 3894 3987 5111 643
Size: 1251 2000 93 1124 1801
Fragments arranged by size:
2000 1801 1251 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2193 3481 3562 3897 6041 2193
Size: 1288 81 335 2144 2421
Fragments arranged by size:
2421 2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4636 4636
Size: 6269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1033 3179 3215 844
Size: 189 2146 36 3898
Fragments arranged by size:
3898 2146 189 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3445 4324 5038 5336 3445
Size: 879 714 298 4378
Fragments arranged by size:
4378 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 532 1750 5973 532
Size: 1218 4223 828
Fragments arranged by size:
4223 1218 828
BsaAI yAC'GTr
Cuts at: 1836 3622 1836
Size: 1786 4483
BsaBI GATnn'nnATC
Cuts at: 6 2666 3783 5820 6
Size: 2660 1117 2037 455
Fragments arranged by size:
2660 2037 1117 455
BsaHI Gr'CG_yC
Cuts at: 151 3700 3948 4650 6146 151
Size: 3549 248 702 1496 274
Fragments arranged by size:
3549 1496 702 274 248
BsaWI w'CCGG_w
Cuts at: 3458 3979 4657 5068 5215 5489 3458
Size: 521 678 411 147 274 4238
Fragments arranged by size:
4238 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1006 1036 1006
Size: 30 6239
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1439 1439
Size: 6269
BseYI C'CCAG_C
Cuts at: 3694 4452 5117 5525 3694
Size: 758 665 408 4438
Fragments arranged by size:
4438 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 683
Size: 6269
BsiEI CG_ry'CG
Cuts at: 104 3811 3857 5087 104
Size: 3707 46 1230 1286
Fragments arranged by size:
3707 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3283 4061 4251 5111 3283
Size: 778 190 860 4441
Fragments arranged by size:
4441 860 778 190
BsmI GAATG_Cn'
Cuts at: 2391 2580 2391
Size: 189 6080
Bsp1286I G_dGCh'C
Cuts at: 643 1894 3283 3894 3987 4061 4251 4313 5111
Size: 1251 1389 611 93 74 190 62 798
Cuts at: 5111 643
Size: 1801
Fragments arranged by size:
1801 1389 1251 798 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 378 3252 4701 378
Size: 2874 1449 1946
Fragments arranged by size:
2874 1946 1449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2790 3319 3533 3835 4216 2790
Size: 529 214 302 381 4843
Fragments arranged by size:
4843 529 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4561 4615 6099 6264 50
Size: 4511 54 1484 165 55
Fragments arranged by size:
4511 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 278 601 4181 278
Size: 323 3580 2366
Fragments arranged by size:
3580 2366 323
BsrFI r'CCGG_y
Cuts at: 4267 4448 4267
Size: 181 6088
BsrGI T'GTAC_A
Cuts at: 669 2723 669
Size: 2054 4215
BssSI C'ACGA_G
Cuts at: 1421 4540 5248 1421
Size: 3119 708 2442
Fragments arranged by size:
3119 2442 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3646 3646
Size: 6269
BstBI TT'CG_AA
Cuts at: 4630 4630
Size: 6269
BstXI CCAn_nnnn'nTGG
Cuts at: 942 942
Size: 6269
BstYI r'GATC_y
Cuts at: 1 115 405 546 880 1596 1953 3174 3294
Size: 114 290 141 334 716 357 1221 120
Cuts at: 3294 3761 3784 4119 4365 4671 4683 4769 5821
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5821 1
Size: 449
Fragments arranged by size:
1221 1052 716 467 449 357 335 334
306 290 246 141 120 114 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1064 1064
Size: 6269
BtgI C'CryG_G
Cuts at: 107 107
Size: 6269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4219 4400 4563 4219
Size: 181 163 5925
Fragments arranged by size:
5925 181 163
BtsI GCAGTG_nn'
Cuts at: 1676 2565 2648 2798 3512 1676
Size: 889 83 150 714 4433
Fragments arranged by size:
4433 889 714 150 83
ClaI AT'CG_AT
Cuts at: 224 2976 224
Size: 2752 3517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3044 3079 3044
Size: 35 6234
DraI TTT'AAA
Cuts at: 875 1490 2354 3268 875
Size: 615 864 914 3876
Fragments arranged by size:
3876 914 864 615
DraIII CAC_nnn'GTG
Cuts at: 1469 1469
Size: 6269
DrdI GACnn_nn'nnGTC
Cuts at: 3975 5319 3975
Size: 1344 4925
EaeI y'GGCC_r
Cuts at: 128 284 3854 4028 4419 4446 5791 128
Size: 156 3570 174 391 27 1345 606
Fragments arranged by size:
3570 1345 606 391 174 156 27
EagI C'GGCC_G
Cuts at: 3854 3854
Size: 6269
EarI CTCTTCn'nnn_
Cuts at: 140 1199 1958 2258 2426 4292 4502 140
Size: 1059 759 300 168 1866 210 1907
Fragments arranged by size:
1907 1866 1059 759 300 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4477 5219 5365 6079 4477
Size: 742 146 714 4667
Fragments arranged by size:
4667 742 714 146
EcoICRI GAG'CTC
Cuts at: 3281 3281
Size: 6269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 844 1033 1928 2239 2365 2617 2945 3179
Size: 359 189 895 311 126 252 328 234
Cuts at: 3179 3215 3231 4094 4526 4879 5541 485
Size: 36 16 863 432 353 662 1213
Fragments arranged by size:
1213 895 863 662 432 359 353 328
311 252 234 189 126 36 16
EcoO109I rG'GnC_Cy
Cuts at: 3226 3408 3226
Size: 182 6087
EcoRI G'AATT_C
Cuts at: 2488 3273 2488
Size: 785 5484
EcoRV GAT'ATC
Cuts at: 905 905
Size: 6269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 490 4288 490
Size: 3798 2471
FspI TGC'GCA
Cuts at: 4050 4050
Size: 6269
HaeII r_GCGC'y
Cuts at: 3703 3951 5181 5952 3703
Size: 248 1230 771 4020
Fragments arranged by size:
4020 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 303 335 531 563 2425 2457 4111 4141 4143
Size: 32 196 32 1862 32 1654 30 2
Cuts at: 4143 4173 303
Size: 30 2399
Fragments arranged by size:
2399 1862 1654 196 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 206 244 3128 3308 206
Size: 38 2884 180 3167
Fragments arranged by size:
3167 2884 180 38
HindIII A'AGCT_T
Cuts at: 2864 2864
Size: 6269
HpaI GTT'AAC
Cuts at: 3128 3128
Size: 6269
KasI G'GCGC_C
Cuts at: 3699 3947 3699
Size: 248 6021
KpnI G_GTAC'C
Cuts at: 3289 3289
Size: 6269
MfeI C'AATT_G
Cuts at: 2900 2900
Size: 6269
MluI A'CGCG_T
Cuts at: 5702 5702
Size: 6269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1122 1643 1771 2610 5028 5212 1122
Size: 521 128 839 2418 184 2179
Fragments arranged by size:
2418 2179 839 521 184 128
MscI TGG'CCA
Cuts at: 286 4030 286
Size: 3744 2525
MslI CAynn'nnrTG
Cuts at: 271 1090 4258 4385 271
Size: 819 3168 127 2155
Fragments arranged by size:
3168 2155 819 127
MspA1I CmG'CkG
Cuts at: 2002 2698 2740 3380 3694 4054 4838 5083 2002
Size: 696 42 640 314 360 784 245 3188
Fragments arranged by size:
3188 784 696 640 360 314 245 42
NaeI GCC'GGC
Cuts at: 4450 4450
Size: 6269
NarI GG'CG_CC
Cuts at: 3700 3948 3700
Size: 248 6021
NcoI C'CATG_G
Cuts at: 107 107
Size: 6269
NgoMIV G'CCGG_C
Cuts at: 4448 4448
Size: 6269
NsiI A_TGCA'T
Cuts at: 234 3137 234
Size: 2903 3366
NspI r_CATG'y
Cuts at: 3322 3822 5425 3322
Size: 500 1603 4166
Fragments arranged by size:
4166 1603 500
PciI A'CATG_T
Cuts at: 5421 5421
Size: 6269
PflMI CCAn_nnn'nTGG
Cuts at: 1141 2024 1141
Size: 883 5386
PmeI GTTT'AAAC
Cuts at: 3268 3268
Size: 6269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 4715
Size: 32 6237
PpuMI rG'GwC_Cy
Cuts at: 3226 3226
Size: 6269
PsiI TTA'TAA
Cuts at: 2164 2164
Size: 6269
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 938 970 2003 2035 938
Size: 32 1033 32 5172
Fragments arranged by size:
5172 1033 32 32
PstI C_TGCA'G
Cuts at: 1284 2106 3316 4001 1284
Size: 822 1210 685 3552
Fragments arranged by size:
3552 1210 822 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6269
PvuII CAG'CTG
Cuts at: 2002 2698 2740 3694 4054 2002
Size: 696 42 954 360 4217
Fragments arranged by size:
4217 954 696 360 42
RsrII CG'GwC_CG
Cuts at: 4464 4464
Size: 6269
SacI G_AGCT'C
Cuts at: 3283 3283
Size: 6269
SalI G'TCGA_C
Cuts at: 242 3306 242
Size: 3064 3205
SapI GCTCTTCn'nnn_
Cuts at: 2258 4292 4502 2258
Size: 2034 210 4025
Fragments arranged by size:
4025 2034 210
SbfI CC_TGCA'GG
Cuts at: 3316 3316
Size: 6269
ScaI AGT'ACT
Cuts at: 5786 5786
Size: 6269
SfcI C'TryA_G
Cuts at: 33 1280 2102 2405 2786 2804 3312 3997 4965
Size: 1247 822 303 381 18 508 685 968
Cuts at: 4965 5156 33
Size: 191 1146
Fragments arranged by size:
1247 1146 968 822 685 508 381 303
191 18
SfoI GGC'GCC
Cuts at: 3701 3949 3701
Size: 248 6021
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
SmaI CCC'GGG
Cuts at: 3291 3291
Size: 6269
SmlI C'TyrA_G
Cuts at: 303 1443 3424 4339 5053 5315 303
Size: 1140 1981 915 714 262 1257
Fragments arranged by size:
1981 1257 1140 915 714 262
SnaBI TAC'GTA
Cuts at: 1836 1836
Size: 6269
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6269
SphI G_CATG'C
Cuts at: 3322 3822 3322
Size: 500 5769
SspI AAT'ATT
Cuts at: 2236 2463 2236
Size: 227 6042
StuI AGG'CCT
Cuts at: 1866 1866
Size: 6269
StyI C'CwwG_G
Cuts at: 107 1100 1199 1257 2825 3403 3757 107
Size: 993 99 58 1568 578 354 2619
Fragments arranged by size:
2619 1568 993 578 354 99 58
SwaI ATTT'AAAT
Cuts at: 2354 2354
Size: 6269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 3852 3985 4444 4653 304
Size: 3548 133 459 209 1920
Fragments arranged by size:
3548 1920 459 209 133
TatI w'GTAC_w
Cuts at: 471 669 1981 2723 5784 471
Size: 198 1312 742 3061 956
Fragments arranged by size:
3061 1312 956 742 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5786 5818 5786
Size: 32 6237
Tth111I GACn'n_nGTC
Cuts at: 1932 4066 1932
Size: 2134 4135
XbaI T'CTAG_A
Cuts at: 3300 3300
Size: 6269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3764 3764
Size: 6269
XmaI C'CCGG_G
Cuts at: 3289 3289
Size: 6269
XmnI GAAnn'nnTTC
Cuts at: 1335 1412 1335
Size: 77 6192
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BspEI BssHII BstEII BstZ17I
EcoNI FseI FspAI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI XhoI ZraI