Restriction map for
pF1KB8364
(Circular) (Six-base) MAPSORT of: pF1KB8364.seq Check: 7105 from: 1 to: 4562
pF1KB8364 4562 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1357 1357
Size: 4562
AatII G_ACGT'C
Cuts at: 1547 1547
Size: 4562
AccI GT'mk_AC
Cuts at: 1600 1600
Size: 4562
Acc65I G'GTAC_C
Cuts at: 1578 1578
Size: 4562
AclI AA'CG_TT
Cuts at: 4402 4402
Size: 4562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 604 1446 2387 2819 3566 3834 150
Size: 454 842 941 432 747 268 878
Fragments arranged by size:
941 878 842 747 454 432 268
AflIII A'CryG_T
Cuts at: 521 1510 1914 3018 3995 521
Size: 989 404 1104 977 1088
Fragments arranged by size:
1104 1088 989 977 404
AgeI A'CCGG_T
Cuts at: 2950 2950
Size: 4562
AleI CACnn'nnGTG
Cuts at: 835 835
Size: 4562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 508
Size: 32 4530
AlwNI CAG_nnn'CTG
Cuts at: 321 618 981 1236 1867 3434 321
Size: 297 363 255 631 1567 1449
Fragments arranged by size:
1567 1449 631 363 297 255
ApaI G_GGCC'C
Cuts at: 194 194
Size: 4562
ApaLI G'TGCA_C
Cuts at: 3332 3332
Size: 4562
ApoI r'AATT_y
Cuts at: 682 1566 3915 4171 682
Size: 884 2349 256 1073
Fragments arranged by size:
2349 1073 884 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4562
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
AvaI C'yCGr_G
Cuts at: 158 213 224 1582 158
Size: 55 11 1358 3138
Fragments arranged by size:
3138 1358 55 11
BamHI G'GATC_C
Cuts at: 1587 1587
Size: 4562
BanI G'GyrC_C
Cuts at: 1578 1992 2240 2275 1578
Size: 414 248 35 3865
Fragments arranged by size:
3865 414 248 35
BanII G_rGCy'C
Cuts at: 194 331 1381 1429 1453 1576 2606 194
Size: 137 1050 48 24 123 1030 2150
Fragments arranged by size:
2150 1050 1030 137 123 48 24
BbeI G_GCGC'C
Cuts at: 1996 2244 1996
Size: 248 4314
BbsI GAAGACnn'nnnn_
Cuts at: 502 1354 502
Size: 852 3710
BbvCI CC'TCA_GC
Cuts at: 890 935 1411 890
Size: 45 476 4041
Fragments arranged by size:
4041 476 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 898 932 898
Size: 34 4528
BciVI GTATCCnnnnn_n'
Cuts at: 2460 3221 4562 2460
Size: 761 1341 2460
Fragments arranged by size:
2460 1341 761
BclI T'GATC_A
Cuts at: 2082 2082
Size: 4562
BglI GCCn_nnn'nGGC
Cuts at: 260 1082 260
Size: 822 3740
BglII A'GATC_T
Cuts at: 821 1025 2077 4114 821
Size: 204 1052 2037 1269
Fragments arranged by size:
2037 1269 1052 204
BlpI GC'TnA_GC
Cuts at: 325 1674 325
Size: 1349 3213
Bme1580I G_kGCm'C
Cuts at: 194 2187 2280 3336 194
Size: 1993 93 1056 1420
Fragments arranged by size:
1993 1420 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1774 1855 2190 4334 1774
Size: 81 335 2144 2002
Fragments arranged by size:
2144 2002 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 4562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 463 484 580 610 757 1042 1120 1195
Size: 102 21 96 30 147 285 78 75
Cuts at: 1195 1312 1333 1413 1437 1464 361
Size: 117 21 80 24 27 3459
Fragments arranged by size:
3459 285 147 117 102 96 80 78
75 30 27 24 21 21
Bpu10I CC'TnA_GC
Cuts at: 890 935 1411 890
Size: 45 476 4041
Fragments arranged by size:
4041 476 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1738 2617 3109 3407 1738
Size: 879 492 298 2893
Fragments arranged by size:
2893 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 4266 460
Size: 3806 756
BsaAI yAC'GTr
Cuts at: 1513 1915 1513
Size: 402 4160
BsaBI GATnn'nnATC
Cuts at: 6 2076 4113 6
Size: 2070 2037 455
Fragments arranged by size:
2070 2037 455
BsaHI Gr'CG_yC
Cuts at: 1544 1993 2241 2943 4439 1544
Size: 449 248 702 1496 1667
Fragments arranged by size:
1667 1496 702 449 248
BsaWI w'CCGG_w
Cuts at: 1040 1751 2272 2950 3224 3371 3782 1040
Size: 711 521 678 274 147 411 1820
Fragments arranged by size:
1820 711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 457 487 799 829 847 877 457
Size: 30 312 30 18 30 4142
Fragments arranged by size:
4142 312 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 811 982 1315 1467 201
Size: 610 171 333 152 3296
Fragments arranged by size:
3296 610 333 171 152
BseYI C'CCAG_C
Cuts at: 985 1987 2745 3322 3818 985
Size: 1002 758 577 496 1729
Fragments arranged by size:
1729 1002 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2104 2150 3358 104
Size: 2000 46 1208 1308
Fragments arranged by size:
2000 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1381 1576 2354 2544 3336 1381
Size: 195 778 190 792 2607
Fragments arranged by size:
2607 792 778 195 190
Bsp1286I G_dGCh'C
Cuts at: 194 331 1381 1429 1453 1576 2187 2280 2354
Size: 137 1050 48 24 123 611 93 74
Cuts at: 2354 2544 2606 3336 194
Size: 190 62 730 1420
Fragments arranged by size:
1420 1050 730 611 190 137 123 93
74 62 48 24
BspEI T'CCGG_A
Cuts at: 3782 3782
Size: 4562
BspHI T'CATG_A
Cuts at: 3738 3738
Size: 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1013 1357 1612 1826 2128 2509 1013
Size: 344 255 214 302 381 3066
Fragments arranged by size:
3066 381 344 302 255 214
BsrBI CCG'CTC
Cuts at: 50 176 863 2854 2908 4392 4557 50
Size: 126 687 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 687 165 126 55 54
BsrDI GCAATG_nn'
Cuts at: 884 1109 2474 884
Size: 225 1365 2972
Fragments arranged by size:
2972 1365 225
BsrFI r'CCGG_y
Cuts at: 1052 1307 2560 2741 2950 1052
Size: 255 1253 181 209 2664
Fragments arranged by size:
2664 1253 255 209 181
BssSI C'ACGA_G
Cuts at: 183 2833 3191 183
Size: 2650 358 1554
Fragments arranged by size:
2650 1554 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1939 1939
Size: 4562
BstBI TT'CG_AA
Cuts at: 2923 2923
Size: 4562
BstEII G'GTnAC_C
Cuts at: 120 120
Size: 4562
BstXI CCAn_nnnn'nTGG
Cuts at: 285 1488 285
Size: 1203 3359
BstYI r'GATC_y
Cuts at: 1 800 821 1025 1587 2054 2077 2412 2658
Size: 799 21 204 562 467 23 335 246
Cuts at: 2658 3670 3756 3768 4114 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 799 562 467 449 346 335 246
204 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 547 778 547
Size: 231 4331
BtgI C'CryG_G
Cuts at: 848 1487 848
Size: 639 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2512 2693 2856 2512
Size: 181 163 4218
Fragments arranged by size:
4218 181 163
BtsI GCAGTG_nn'
Cuts at: 1805 1805
Size: 4562
DraI TTT'AAA
Cuts at: 1561 1561
Size: 4562
DrdI GACnn_nn'nnGTC
Cuts at: 129 2268 3126 129
Size: 2139 858 1565
Fragments arranged by size:
2139 1565 858
EaeI y'GGCC_r
Cuts at: 1139 2147 2321 2712 2739 4084 1139
Size: 1008 174 391 27 1345 1617
Fragments arranged by size:
1617 1345 1008 391 174 27
EagI C'GGCC_G
Cuts at: 2147 2147
Size: 4562
EarI CTCTTCn'nnn_
Cuts at: 788 2585 2795 788
Size: 1797 210 2555
Fragments arranged by size:
2555 1797 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 610 2770 3080 3226 4372 610
Size: 2160 310 146 1146 800
Fragments arranged by size:
2160 1146 800 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1574 1379
Size: 195 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 361 463 484 580 604 610 757 1042
Size: 211 102 21 96 24 6 147 285
Cuts at: 1042 1120 1195 1312 1333 1413 1437 1446 1464
Size: 78 75 117 21 80 24 9 18
Cuts at: 1464 2387 2819 3566 3834 150
Size: 923 432 747 268 878
Fragments arranged by size:
923 878 747 432 285 268 211 147
117 102 96 80 78 75 24 24
21 21 18 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1552 1552
Size: 4562
EcoO109I rG'GnC_Cy
Cuts at: 1074 1392 1701 1074
Size: 318 309 3935
Fragments arranged by size:
3935 318 309
EcoRI G'AATT_C
Cuts at: 1566 1566
Size: 4562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4562
FspI TGC'GCA
Cuts at: 370 2343 370
Size: 1973 2589
FspAI rTGC'GCAy
Cuts at: 370 370
Size: 4562
HaeII r_GCGC'y
Cuts at: 1996 2244 3266 4245 1996
Size: 248 1022 979 2313
Fragments arranged by size:
2313 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 724 756 799 831 2404 2434 2436 2466 724
Size: 32 43 32 1573 30 2 30 2820
Fragments arranged by size:
2820 1573 43 32 32 30 30 2
HincII GTy'rAC
Cuts at: 312 1601 312
Size: 1289 3273
KasI G'GCGC_C
Cuts at: 1992 2240 1992
Size: 248 4314
KpnI G_GTAC'C
Cuts at: 1582 1582
Size: 4562
MluI A'CGCG_T
Cuts at: 3995 3995
Size: 4562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3233 3417 3233
Size: 184 4378
MscI TGG'CCA
Cuts at: 1141 2323 1141
Size: 1182 3380
MslI CAynn'nnrTG
Cuts at: 118 835 998 2551 2678 118
Size: 717 163 1553 127 2002
Fragments arranged by size:
2002 1553 717 163 127
MspA1I CmG'CkG
Cuts at: 281 558 1191 1218 1358 1673 1987 2347 3360
Size: 277 633 27 140 315 314 360 1013
Cuts at: 3360 3605 281
Size: 245 1238
Fragments arranged by size:
1238 1013 633 360 315 314 277 245
140 27
NaeI GCC'GGC
Cuts at: 1054 1309 2743 1054
Size: 255 1434 2873
Fragments arranged by size:
2873 1434 255
NarI GG'CG_CC
Cuts at: 1993 2241 1993
Size: 248 4314
NcoI C'CATG_G
Cuts at: 848 848
Size: 4562
NgoMIV G'CCGG_C
Cuts at: 1052 1307 2741 1052
Size: 255 1434 2873
Fragments arranged by size:
2873 1434 255
NspI r_CATG'y
Cuts at: 525 612 951 1615 2115 3022 525
Size: 87 339 664 500 907 2065
Fragments arranged by size:
2065 907 664 500 339 87
PciI A'CATG_T
Cuts at: 521 3018 521
Size: 2497 2065
PfoI T'CCnGG_A
Cuts at: 338 440 803 338
Size: 102 363 4097
Fragments arranged by size:
4097 363 102
PmeI GTTT'AAAC
Cuts at: 1561 1561
Size: 4562
PmlI CAC'GTG
Cuts at: 1513 1513
Size: 4562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4530
PspOMI G'GGCC_C
Cuts at: 190 190
Size: 4562
PspXI vC'TCGA_Gb
Cuts at: 213 213
Size: 4562
PstI C_TGCA'G
Cuts at: 488 815 977 1190 1609 2294 488
Size: 327 162 213 419 685 2756
Fragments arranged by size:
2756 685 419 327 213 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4562
PvuII CAG'CTG
Cuts at: 558 1218 1987 2347 558
Size: 660 769 360 2773
Fragments arranged by size:
2773 769 660 360
RsrII CG'GwC_CG
Cuts at: 2757 2757
Size: 4562
SacI G_AGCT'C
Cuts at: 1381 1576 1381
Size: 195 4367
SalI G'TCGA_C
Cuts at: 1599 1599
Size: 4562
SapI GCTCTTCn'nnn_
Cuts at: 2585 2795 2585
Size: 210 4352
SbfI CC_TGCA'GG
Cuts at: 1609 1609
Size: 4562
ScaI AGT'ACT
Cuts at: 4079 4079
Size: 4562
SfcI C'TryA_G
Cuts at: 33 484 811 973 1186 1278 1416 1605 2290
Size: 451 327 162 213 92 138 189 685
Cuts at: 2290 3283 3474 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 451 327 213 191 189
162 138 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 1082 1082
Size: 4562
SfoI GGC'GCC
Cuts at: 1994 2242 1994
Size: 248 4314
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
SmaI CCC'GGG
Cuts at: 160 1584 160
Size: 1424 3138
SmlI C'TyrA_G
Cuts at: 213 1717 2632 3124 3386 213
Size: 1504 915 492 262 1389
Fragments arranged by size:
1504 1389 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4562
SphI G_CATG'C
Cuts at: 1615 2115 1615
Size: 500 4062
StyI C'CwwG_G
Cuts at: 848 1553 1696 2050 848
Size: 705 143 354 3360
Fragments arranged by size:
3360 705 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 388 775 2145 2278 2737 2946 388
Size: 387 1370 133 459 209 2004
Fragments arranged by size:
2004 1370 459 387 209 133
TatI w'GTAC_w
Cuts at: 4077 4077
Size: 4562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 4079 4111 457
Size: 32 3590 32 908
Fragments arranged by size:
3590 908 32 32
Tth111I GACn'n_nGTC
Cuts at: 2359 2359
Size: 4562
XbaI T'CTAG_A
Cuts at: 1593 1593
Size: 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1203 2057 1203
Size: 854 3708
XhoI C'TCGA_G
Cuts at: 213 213
Size: 4562
XmaI C'CCGG_G
Cuts at: 158 1582 158
Size: 1424 3138
ZraI GAC'GTC
Cuts at: 1545 1545
Size: 4562
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI AvrII BaeI BfrBI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PpuMI PshAI PsiI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI