Restriction map for
pF1KB8358
(Circular) (Six-base) MAPSORT of: pF1KB8358.seq Check: 9017 from: 1 to: 4145
pF1KB8358 4145 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 344 1183 344
Size: 839 3306
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1970 2402 3149 3417 733
Size: 1237 432 747 268 1461
Fragments arranged by size:
1461 1237 747 432 268
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AleI CACnn'nnGTG
Cuts at: 199 199
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaI G_GGCC'C
Cuts at: 556 556
Size: 4145
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 514 1149 3498 3754 514
Size: 635 2349 256 905
Fragments arranged by size:
2349 905 635 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 1165 1165
Size: 4145
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 526 828 847 1161 1575 1823 1858 526
Size: 302 19 314 414 248 35 2813
Fragments arranged by size:
2813 414 314 302 248 35 19
BanII G_rGCy'C
Cuts at: 480 556 1159 2189 480
Size: 76 603 1030 2436
Fragments arranged by size:
2436 1030 603 76
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4111
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BfrBI ATG'CAT
Cuts at: 111 633 111
Size: 522 3623
BglI GCCn_nnn'nGGC
Cuts at: 1030 1030
Size: 4145
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 307 1257 307
Size: 950 3195
Bme1580I G_kGCm'C
Cuts at: 165 556 671 833 850 1130 1770 1863 2919
Size: 391 115 162 17 280 640 93 1056
Cuts at: 2919 165
Size: 1391
Fragments arranged by size:
1391 1056 640 391 280 162 115 93
17
BmgBI CAC'GTC
Cuts at: 279 279
Size: 4145
BmrI ACTGGGnnnn_n'
Cuts at: 962 1357 1438 1773 3917 962
Size: 395 81 335 2144 1190
Fragments arranged by size:
2144 1190 395 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 580 968 268
Size: 312 388 3445
Fragments arranged by size:
3445 388 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1321 2200 2692 2990 766
Size: 555 879 492 298 1921
Fragments arranged by size:
1921 879 555 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 244 3849 244
Size: 3605 540
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 130 1659 3696 6
Size: 124 1529 2037 455
Fragments arranged by size:
2037 1529 455 124
BsaHI Gr'CG_yC
Cuts at: 188 865 1576 1824 2526 4022 188
Size: 677 711 248 702 1496 311
Fragments arranged by size:
1496 711 702 677 311 248
BsaWI w'CCGG_w
Cuts at: 1334 1855 2533 2807 2954 3365 1334
Size: 521 678 274 147 411 2114
Fragments arranged by size:
2114 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 679 709 985 1015 679
Size: 30 276 30 3809
Fragments arranged by size:
3809 276 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 915 918 915
Size: 3 4142
BseYI C'CCAG_C
Cuts at: 612 670 898 937 942 1570 2328 2905 3401
Size: 58 228 39 5 628 758 577 496
Cuts at: 3401 612
Size: 1356
Fragments arranged by size:
1356 758 628 577 496 228 58 39
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4145
BsiEI CG_ry'CG
Cuts at: 104 301 1687 1733 2941 104
Size: 197 1386 46 1208 1308
Fragments arranged by size:
1386 1308 1208 197 46
BsiHKAI G_wGCw'C
Cuts at: 1159 1937 2127 2919 1159
Size: 778 190 792 2385
Fragments arranged by size:
2385 792 778 190
BsmI GAATG_Cn'
Cuts at: 213 213
Size: 4145
Bsp1286I G_dGCh'C
Cuts at: 165 480 556 671 833 850 1130 1159 1770
Size: 315 76 115 162 17 280 29 611
Cuts at: 1770 1863 1937 2127 2189 2919 165
Size: 93 74 190 62 730 1391
Fragments arranged by size:
1391 730 611 315 280 190 162 115
93 76 74 62 29 17
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 238 273 1195 1409 1711 2092 238
Size: 35 922 214 302 381 2291
Fragments arranged by size:
2291 922 381 302 214 35
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2057 2057
Size: 4145
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 230 1522 230
Size: 1292 2853
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstXI CCAn_nnnn'nTGG
Cuts at: 201 201
Size: 4145
BstYI r'GATC_y
Cuts at: 1 1170 1637 1660 1995 2241 3253 3339 3351
Size: 1169 467 23 335 246 1012 86 12
Cuts at: 3351 3697 1
Size: 346 449
Fragments arranged by size:
1169 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 447 824 957 975 447
Size: 377 133 18 3617
Fragments arranged by size:
3617 377 133 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 621 723 2095 2276 2439 621
Size: 102 1372 181 163 2327
Fragments arranged by size:
2327 1372 181 163 102
BtsI GCAGTG_nn'
Cuts at: 1388 1388
Size: 4145
DraI TTT'AAA
Cuts at: 369 1144 369
Size: 775 3370
DrdI GACnn_nn'nnGTC
Cuts at: 283 1851 2709 283
Size: 1568 858 1719
Fragments arranged by size:
1719 1568 858
EaeI y'GGCC_r
Cuts at: 298 1730 1904 2295 2322 3667 298
Size: 1432 174 391 27 1345 776
Fragments arranged by size:
1432 1345 776 391 174 27
EagI C'GGCC_G
Cuts at: 298 1730 298
Size: 1432 2713
EarI CTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 777 2353 2663 2809 3955 777
Size: 1576 310 146 1146 967
Fragments arranged by size:
1576 1146 967 310 146
EcoICRI GAG'CTC
Cuts at: 1157 1157
Size: 4145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 580 733 968 1970 2402 3149 3417 268
Size: 312 153 235 1002 432 747 268 996
Fragments arranged by size:
1002 996 747 432 312 268 235 153
EcoNI CCTnn'n_nnAGG
Cuts at: 418 418
Size: 4145
EcoO109I rG'GnC_Cy
Cuts at: 423 552 553 1284 423
Size: 129 1 731 3284
Fragments arranged by size:
3284 731 129 1
EcoRI G'AATT_C
Cuts at: 514 1149 514
Size: 635 3510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1121 1579 1827 2849 3828 1121
Size: 458 248 1022 979 1438
Fragments arranged by size:
1438 1022 979 458 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 1987 2017 2019 2049 862
Size: 32 1093 30 2 30 2958
Fragments arranged by size:
2958 1093 32 30 30 2
HincII GTy'rAC
Cuts at: 1113 1184 1113
Size: 71 4074
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3000 2816
Size: 184 3961
MscI TGG'CCA
Cuts at: 1906 1906
Size: 4145
MslI CAynn'nnrTG
Cuts at: 199 730 2134 2261 199
Size: 531 1404 127 2083
Fragments arranged by size:
2083 1404 531 127
MspA1I CmG'CkG
Cuts at: 303 449 860 1042 1256 1570 1930 2943 3188
Size: 146 411 182 214 314 360 1013 245
Cuts at: 3188 303
Size: 1260
Fragments arranged by size:
1260 1013 411 360 314 245 214 182
146
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 957 975 957
Size: 18 4127
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NsiI A_TGCA'T
Cuts at: 113 635 113
Size: 522 3623
NspI r_CATG'y
Cuts at: 1198 1698 2605 1198
Size: 500 907 2738
Fragments arranged by size:
2738 907 500
PasI CC'CwG_GG
Cuts at: 358 358
Size: 4145
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PflMI CCAn_nnn'nTGG
Cuts at: 958 958
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PpuMI rG'GwC_Cy
Cuts at: 423 423
Size: 4145
PshAI GACnn'nnGTC
Cuts at: 421 421
Size: 4145
PspOMI G'GGCC_C
Cuts at: 552 552
Size: 4145
PstI C_TGCA'G
Cuts at: 1073 1192 1877 1073
Size: 119 685 3341
Fragments arranged by size:
3341 685 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 1042 1570 1930 1042
Size: 528 360 3257
Fragments arranged by size:
3257 528 360
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 1159 1159
Size: 4145
SacII CC_GC'GG
Cuts at: 450 450
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 1069 1188 1873 2866 3057 33
Size: 1036 119 685 993 191 1121
Fragments arranged by size:
1121 1036 993 685 191 119
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 781 1300 2215 2707 2969 781
Size: 519 915 492 262 1957
Fragments arranged by size:
1957 915 519 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
StyI C'CwwG_G
Cuts at: 957 975 1279 1633 957
Size: 18 304 354 3469
Fragments arranged by size:
3469 354 304 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1006 1728 1861 2320 2529 1006
Size: 722 133 459 209 2622
Fragments arranged by size:
2622 722 459 209 133
TatI w'GTAC_w
Cuts at: 677 3660 677
Size: 2983 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 985 1017 3662 3694 985
Size: 32 2645 32 1436
Fragments arranged by size:
2645 1436 32 32
Tth111I GACn'n_nGTC
Cuts at: 340 1942 340
Size: 1602 2543
XbaI T'CTAG_A
Cuts at: 1176 1176
Size: 4145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 198 1640 198
Size: 1442 2703
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PfoI PmlI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI