Restriction map for
pF1KB8336
(Circular) (Six-base) MAPSORT of: pF1KB8336.seq Check: 8826 from: 1 to: 4586
pF1KB8336 4586 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 1624 1624
Size: 4586
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 821 1005 1089 1173 1257 1341 1509 2411
Size: 108 184 84 84 84 84 168 902
Cuts at: 2411 2843 3590 3858 713
Size: 432 747 268 1441
Fragments arranged by size:
1441 902 747 432 268 184 168 108
84 84 84 84
AflIII A'CryG_T
Cuts at: 1256 1340 1938 3042 4019 1256
Size: 84 598 1104 977 1823
Fragments arranged by size:
1823 1104 977 598 84
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 339 372 399 498 1364 1511 1891 3458 339
Size: 33 27 99 866 147 380 1567 1467
Fragments arranged by size:
1567 1467 866 380 147 99 33 27
ApaLI G'TGCA_C
Cuts at: 1225 3356 1225
Size: 2131 2455
ApoI r'AATT_y
Cuts at: 847 1590 3939 4195 847
Size: 743 2349 256 1238
Fragments arranged by size:
2349 1238 743 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 346 783 955 1606 346
Size: 437 172 651 3326
Fragments arranged by size:
3326 651 437 172
BamHI G'GATC_C
Cuts at: 516 1475 1559 1611 516
Size: 959 84 52 3491
Fragments arranged by size:
3491 959 84 52
BanI G'GyrC_C
Cuts at: 778 1602 2016 2264 2299 778
Size: 824 414 248 35 3065
Fragments arranged by size:
3065 824 414 248 35
BanII G_rGCy'C
Cuts at: 136 441 533 1453 1600 2630 136
Size: 305 92 920 147 1030 2092
Fragments arranged by size:
2092 1030 920 305 147 92
BbeI G_GCGC'C
Cuts at: 2020 2268 2020
Size: 248 4338
BbsI GAAGACnn'nnnn_
Cuts at: 848 976 991 848
Size: 128 15 4443
Fragments arranged by size:
4443 128 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 308 342 1020 1054 308
Size: 34 678 34 3840
Fragments arranged by size:
3840 678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 2106 2106
Size: 4586
BglII A'GATC_T
Cuts at: 683 2101 4138 683
Size: 1418 2037 1131
Fragments arranged by size:
2037 1418 1131
BlpI GC'TnA_GC
Cuts at: 1368 1536 1698 1368
Size: 168 162 4256
Fragments arranged by size:
4256 168 162
Bme1580I G_kGCm'C
Cuts at: 1229 2211 2304 3360 1229
Size: 982 93 1056 2455
Fragments arranged by size:
2455 1056 982 93
BmrI ACTGGGnnnn_n'
Cuts at: 130 1798 1879 2214 4358 130
Size: 1668 81 335 2144 358
Fragments arranged by size:
2144 1668 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 372 672 2953 372
Size: 300 2281 2005
Fragments arranged by size:
2281 2005 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 409 1169 1421 394
Size: 15 760 252 3559
Fragments arranged by size:
3559 760 252 15
Bpu10I CC'TnA_GC
Cuts at: 130 130
Size: 4586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2641 3133 3431 1762
Size: 879 492 298 2917
Fragments arranged by size:
2917 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 586 4290 586
Size: 3704 882
BsaAI yAC'GTr
Cuts at: 1939 1939
Size: 4586
BsaBI GATnn'nnATC
Cuts at: 6 1564 2100 4137 6
Size: 1558 536 2037 455
Fragments arranged by size:
2037 1558 536 455
BsaHI Gr'CG_yC
Cuts at: 2017 2265 2967 4463 2017
Size: 248 702 1496 2140
Fragments arranged by size:
2140 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1775 2296 2974 3248 3395 3806 1775
Size: 521 678 274 147 411 2555
Fragments arranged by size:
2555 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 864 894 802
Size: 30 32 30 4494
Fragments arranged by size:
4494 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 295 1443 193
Size: 102 1148 3336
Fragments arranged by size:
3336 1148 102
BseYI C'CCAG_C
Cuts at: 1043 1211 2011 2769 3346 3842 1043
Size: 168 800 758 577 496 1787
Fragments arranged by size:
1787 800 758 577 496 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 1524 233
Size: 1291 3295
BsiEI CG_ry'CG
Cuts at: 104 2128 2174 3382 104
Size: 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1229 1453 1469 1600 2378 2568 3360 1033
Size: 196 224 16 131 778 190 792 2259
Fragments arranged by size:
2259 792 778 224 196 190 131 16
Bsp1286I G_dGCh'C
Cuts at: 136 441 533 1033 1229 1453 1469 1600 2211
Size: 305 92 500 196 224 16 131 611
Cuts at: 2211 2304 2378 2568 2630 3360 136
Size: 93 74 190 62 730 1362
Fragments arranged by size:
1362 730 611 500 305 224 196 190
131 93 92 74 62 16
BspEI T'CCGG_A
Cuts at: 3806 3806
Size: 4586
BspHI T'CATG_A
Cuts at: 3762 3762
Size: 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1636 1850 2152 2533 1636
Size: 214 302 381 3689
Fragments arranged by size:
3689 381 302 214
BsrBI CCG'CTC
Cuts at: 50 455 1239 2878 2932 4416 4581 50
Size: 405 784 1639 54 1484 165 55
Fragments arranged by size:
1639 1484 784 405 165 55 54
BsrDI GCAATG_nn'
Cuts at: 230 2498 230
Size: 2268 2318
BsrFI r'CCGG_y
Cuts at: 2584 2765 2974 2584
Size: 181 209 4196
Fragments arranged by size:
4196 209 181
BssSI C'ACGA_G
Cuts at: 298 2857 3215 298
Size: 2559 358 1669
Fragments arranged by size:
2559 1669 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1963 1963
Size: 4586
BstBI TT'CG_AA
Cuts at: 2947 2947
Size: 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 143 143
Size: 4586
BstYI r'GATC_y
Cuts at: 1 419 483 516 683 710 1475 1559 1611
Size: 418 64 33 167 27 765 84 52
Cuts at: 1611 2078 2101 2436 2682 3694 3780 3792 4138
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4138 1
Size: 449
Fragments arranged by size:
1012 765 467 449 418 346 335 246
167 86 84 64 52 33 27 23
12
Bsu36I CC'TnA_GG
Cuts at: 647 647
Size: 4586
BtgI C'CryG_G
Cuts at: 107 142 107
Size: 35 4551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2536 2717 2880 2536
Size: 181 163 4242
Fragments arranged by size:
4242 181 163
BtsI GCAGTG_nn'
Cuts at: 1829 1829
Size: 4586
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 2292 3150 2292
Size: 858 3728
EaeI y'GGCC_r
Cuts at: 110 765 805 1347 2171 2345 2736 2763 4108
Size: 655 40 542 824 174 391 27 1345
Cuts at: 4108 110
Size: 588
Fragments arranged by size:
1345 824 655 588 542 391 174 40
27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 143 179 895 2609 2819 143
Size: 36 716 1714 210 1910
Fragments arranged by size:
1910 1714 716 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 224 2794 3104 3250 4396 224
Size: 2570 310 146 1146 414
Fragments arranged by size:
2570 1146 414 310 146
EcoICRI GAG'CTC
Cuts at: 1451 1598 1451
Size: 147 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 409 713 821 1005 1089 1169 1173 1257
Size: 15 304 108 184 84 80 4 84
Cuts at: 1257 1341 1421 1509 2411 2843 3590 3858 394
Size: 84 80 88 902 432 747 268 1122
Fragments arranged by size:
1122 902 747 432 304 268 184 108
88 84 84 84 80 80 15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 202 202
Size: 4586
EcoO109I rG'GnC_Cy
Cuts at: 303 733 905 1725 303
Size: 430 172 820 3164
Fragments arranged by size:
3164 820 430 172
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4586
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 955 2020 2268 3290 4269 955
Size: 1065 248 1022 979 1272
Fragments arranged by size:
1272 1065 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 864 896 963 995 1368 1400 2428
Size: 32 561 32 67 32 373 32 1028
Cuts at: 2428 2458 2460 2490 271
Size: 30 2 30 2367
Fragments arranged by size:
2367 1028 561 373 67 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1625 1625
Size: 4586
KasI G'GCGC_C
Cuts at: 2016 2264 2016
Size: 248 4338
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MluI A'CGCG_T
Cuts at: 1256 1340 4019 1256
Size: 84 2679 1823
Fragments arranged by size:
2679 1823 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1404 3257 3441 1404
Size: 1853 184 2549
Fragments arranged by size:
2549 1853 184
MscI TGG'CCA
Cuts at: 112 807 2347 112
Size: 695 1540 2351
Fragments arranged by size:
2351 1540 695
MslI CAynn'nnrTG
Cuts at: 1416 1570 2575 2702 1416
Size: 154 1005 127 3300
Fragments arranged by size:
3300 1005 154 127
MspA1I CmG'CkG
Cuts at: 469 1221 1697 2011 2371 3384 3629 469
Size: 752 476 314 360 1013 245 1426
Fragments arranged by size:
1426 1013 752 476 360 314 245
NaeI GCC'GGC
Cuts at: 2767 2767
Size: 4586
NarI GG'CG_CC
Cuts at: 2017 2265 2017
Size: 248 4338
NcoI C'CATG_G
Cuts at: 107 107
Size: 4586
NdeI CA'TA_TG
Cuts at: 993 993
Size: 4586
NgoMIV G'CCGG_C
Cuts at: 2765 2765
Size: 4586
NruI TCG'CGA
Cuts at: 920 920
Size: 4586
NspI r_CATG'y
Cuts at: 641 1639 2139 3046 641
Size: 998 500 907 2181
Fragments arranged by size:
2181 998 907 500
PasI CC'CwG_GG
Cuts at: 204 204
Size: 4586
PciI A'CATG_T
Cuts at: 3042 3042
Size: 4586
PfoI T'CCnGG_A
Cuts at: 519 519
Size: 4586
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 809 3725 3757 777
Size: 32 2916 32 1606
Fragments arranged by size:
2916 1606 32 32
PpuMI rG'GwC_Cy
Cuts at: 905 905
Size: 4586
PsiI TTA'TAA
Cuts at: 1499 1499
Size: 4586
PspXI vC'TCGA_Gb
Cuts at: 783 955 783
Size: 172 4414
PstI C_TGCA'G
Cuts at: 344 1003 1633 2318 344
Size: 659 630 685 2612
Fragments arranged by size:
2612 685 659 630
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 2011 2371 2011
Size: 360 4226
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 1453 1600 1453
Size: 147 4439
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 4103 4103
Size: 4586
SexAI A'CCwGG_T
Cuts at: 1049 1049
Size: 4586
SfcI C'TryA_G
Cuts at: 33 340 999 1629 2314 3307 3498 33
Size: 307 659 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 659 630 307 191
SfoI GGC'GCC
Cuts at: 2018 2266 2018
Size: 248 4338
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 1608 1608
Size: 4586
SmlI C'TyrA_G
Cuts at: 783 955 1741 2656 3148 3410 783
Size: 172 786 915 492 262 1959
Fragments arranged by size:
1959 915 786 492 262 172
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
StuI AGG'CCT
Cuts at: 1102 1438 1522 1102
Size: 336 84 4166
Fragments arranged by size:
4166 336 84
StyI C'CwwG_G
Cuts at: 107 359 615 1720 2074 107
Size: 252 256 1105 354 2619
Fragments arranged by size:
2619 1105 354 256 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 391 420 718 1132 1225 2169 2302 2761 2970
Size: 29 298 414 93 944 133 459 209
Cuts at: 2970 391
Size: 2007
Fragments arranged by size:
2007 944 459 414 298 209 133 93
29
TatI w'GTAC_w
Cuts at: 4101 4101
Size: 4586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4103 4135 4103
Size: 32 4554
Tth111I GACn'n_nGTC
Cuts at: 693 2383 693
Size: 1690 2896
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2081 2081
Size: 4586
XhoI C'TCGA_G
Cuts at: 783 955 783
Size: 172 4414
XmaI C'CCGG_G
Cuts at: 1606 1606
Size: 4586
XmnI GAAnn'nnTTC
Cuts at: 934 1018 1102 1270 1522 934
Size: 84 84 168 252 3998
Fragments arranged by size:
3998 252 168 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PasI PciI PfoI
PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NsiI PacI PflMI PmlI PshAI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI