Restriction map for
pF1KB8327
(Circular) (Six-base) MAPSORT of: pF1KB8327.seq Check: 6833 from: 1 to: 3875
pF1KB8327 3875 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2879 3147 1700
Size: 432 747 268 2428
Fragments arranged by size:
2428 747 432 268
AflIII A'CryG_T
Cuts at: 1227 2331 3308 1227
Size: 1104 977 1794
Fragments arranged by size:
1794 1104 977
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AhdI GACnn_n'nnGTC
Cuts at: 237 237
Size: 3875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 632 632
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 1180 2747 1180
Size: 1567 2308
ApaI G_GGCC'C
Cuts at: 432 432
Size: 3875
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 879 3228 3484 879
Size: 2349 256 1270
Fragments arranged by size:
2349 1270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 242 284 369 895 242
Size: 42 85 526 3222
Fragments arranged by size:
3222 526 85 42
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 171 432 889 1919 171
Size: 261 457 1030 2127
Fragments arranged by size:
2127 1030 457 261
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BbvCI CC'TCA_GC
Cuts at: 757 809 757
Size: 52 3823
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2534 3875 1773
Size: 761 1341 1773
Fragments arranged by size:
1773 1341 761
BclI T'GATC_A
Cuts at: 299 1395 299
Size: 1096 2779
BglI GCCn_nnn'nGGC
Cuts at: 155 155
Size: 3875
BglII A'GATC_T
Cuts at: 650 1390 3427 650
Size: 740 2037 1098
Fragments arranged by size:
2037 1098 740
BlpI GC'TnA_GC
Cuts at: 145 987 145
Size: 842 3033
Bme1580I G_kGCm'C
Cuts at: 432 1500 1593 2649 432
Size: 1068 93 1056 1658
Fragments arranged by size:
1658 1068 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 259 1087 1168 1503 3647 259
Size: 828 81 335 2144 487
Fragments arranged by size:
2144 828 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 584 185
Size: 399 3476
Bpu10I CC'TnA_GC
Cuts at: 757 809 757
Size: 52 3823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2422 2720 1051
Size: 879 492 298 2206
Fragments arranged by size:
2206 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 484 3579 484
Size: 3095 780
BsaAI yAC'GTr
Cuts at: 218 1228 218
Size: 1010 2865
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 739 742 781 379
Size: 360 3 39 3473
Fragments arranged by size:
3473 360 39 3
BseYI C'CCAG_C
Cuts at: 225 293 309 1300 2058 2635 3131 225
Size: 68 16 991 758 577 496 969
Fragments arranged by size:
991 969 758 577 496 68 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 874 192
Size: 682 3193
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 889 1667 1857 2649 889
Size: 778 190 792 2115
Fragments arranged by size:
2115 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 171 432 889 1500 1593 1667 1857 1919 2649
Size: 261 457 611 93 74 190 62 730
Cuts at: 2649 171
Size: 1397
Fragments arranged by size:
1397 730 611 457 261 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 925 1139 1441 1822 230
Size: 695 214 302 381 2283
Fragments arranged by size:
2283 695 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1787 1787
Size: 3875
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 650 900 1367 1390 1725 1971 2983 3069
Size: 649 250 467 23 335 246 1012 86
Cuts at: 3069 3081 3427 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 250 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 582 699 719 107
Size: 475 117 20 3263
Fragments arranged by size:
3263 475 117 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 342 863 1118 342
Size: 521 255 3099
Fragments arranged by size:
3099 521 255
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 507 1460 1634 2025 2052 3397 507
Size: 953 174 391 27 1345 985
Fragments arranged by size:
1345 985 953 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 609 2083 2393 2539 3685 127
Size: 482 1474 310 146 1146 317
Fragments arranged by size:
1474 1146 482 317 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 584 1700 2132 2879 3147 185
Size: 399 1116 432 747 268 913
Fragments arranged by size:
1116 913 747 432 399 268
EcoO109I rG'GnC_Cy
Cuts at: 428 1014 428
Size: 586 3289
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 283 480 1309 1557 2579 3558 283
Size: 197 829 248 1022 979 600
Fragments arranged by size:
1022 979 829 600 248 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 158 190 223 255 445 449 477 481 1717
Size: 32 33 32 190 4 28 4 1236
Cuts at: 1717 1747 1749 1779 158
Size: 30 2 30 2254
Fragments arranged by size:
2254 1236 190 33 32 32 30 30
28 4 4 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 467 2546 2730 467
Size: 2079 184 1612
Fragments arranged by size:
2079 1612 184
MscI TGG'CCA
Cuts at: 509 1636 509
Size: 1127 2748
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 144 297 761 986 1300 1660 2673 2918 144
Size: 153 464 225 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 464 360 314 245 225 153
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 699 107
Size: 592 3283
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 843 928 1428 2335 843
Size: 85 500 907 2383
Fragments arranged by size:
2383 907 500 85
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PflMI CCAn_nnn'nTGG
Cuts at: 552 552
Size: 3875
PfoI T'CCnGG_A
Cuts at: 269 663 269
Size: 394 3481
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PspOMI G'GGCC_C
Cuts at: 428 428
Size: 3875
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 144 297 761 1300 1660 144
Size: 153 464 539 360 2359
Fragments arranged by size:
2359 539 464 360 153
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 918 1603 2596 2787 33
Size: 885 685 993 191 1121
Fragments arranged by size:
1121 993 885 685 191
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 244 897 244
Size: 653 3222
SmlI C'TyrA_G
Cuts at: 1030 1945 2437 2699 1030
Size: 915 492 262 2206
Fragments arranged by size:
2206 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 699 1009 1363 107
Size: 592 310 354 2619
Fragments arranged by size:
2619 592 354 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 575 627 876 1458 1591 2050 2259 217
Size: 358 52 249 582 133 459 209 1833
Fragments arranged by size:
1833 582 459 358 249 209 133 52
TatI w'GTAC_w
Cuts at: 120 3390 120
Size: 3270 605
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 265 1672 265
Size: 1407 2468
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 242 895 242
Size: 653 3222
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI