Restriction map for
pF1KB8289
(Circular) (Six-base) MAPSORT of: pF1KB8289.seq Check: 7209 from: 1 to: 4019
pF1KB8289 4019 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1057 1057
Size: 4019
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 1844 2276 3023 3291 752
Size: 1092 432 747 268 1480
Fragments arranged by size:
1480 1092 747 432 268
AflIII A'CryG_T
Cuts at: 113 1371 2475 3452 113
Size: 1258 1104 977 680
Fragments arranged by size:
1258 1104 977 680
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AleI CACnn'nnGTG
Cuts at: 839 929 839
Size: 90 3929
AlwNI CAG_nnn'CTG
Cuts at: 1324 2891 1324
Size: 1567 2452
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 1023 3372 3628 1023
Size: 2349 256 1414
Fragments arranged by size:
2349 1414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 407 1039 407
Size: 632 3387
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 782 1035 1449 1697 1732 782
Size: 253 414 248 35 3069
Fragments arranged by size:
3069 414 253 248 35
BanII G_rGCy'C
Cuts at: 248 385 539 905 1033 2063 248
Size: 137 154 366 128 1030 2204
Fragments arranged by size:
2204 1030 366 154 137 128
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BbvCI CC'TCA_GC
Cuts at: 436 436
Size: 4019
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglI GCCn_nnn'nGGC
Cuts at: 616 673 616
Size: 57 3962
BglII A'GATC_T
Cuts at: 1534 3571 1534
Size: 2037 1982
BlpI GC'TnA_GC
Cuts at: 538 1131 538
Size: 593 3426
Bme1580I G_kGCm'C
Cuts at: 1644 1737 2793 1644
Size: 93 1056 2870
Fragments arranged by size:
2870 1056 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 4019
BmrI ACTGGGnnnn_n'
Cuts at: 1231 1312 1647 3791 1231
Size: 81 335 2144 1459
Fragments arranged by size:
2144 1459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 399 2386 399
Size: 1987 2032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 427 211
Size: 216 3803
Bpu10I CC'TnA_GC
Cuts at: 436 533 436
Size: 97 3922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 190 256 421 3723 190
Size: 66 165 3302 486
Fragments arranged by size:
3302 486 165 66
BsaAI yAC'GTr
Cuts at: 1372 1372
Size: 4019
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 208
Size: 4019
BseYI C'CCAG_C
Cuts at: 491 1444 2202 2779 3275 491
Size: 953 758 577 496 1235
Fragments arranged by size:
1235 953 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1561 1607 2815 104
Size: 1457 46 1208 1308
Fragments arranged by size:
1457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 385 539 862 905 1033 1811 2001 2793 385
Size: 154 323 43 128 778 190 792 1611
Fragments arranged by size:
1611 792 778 323 190 154 128 43
Bsp1286I G_dGCh'C
Cuts at: 248 385 539 862 905 1033 1644 1737 1811
Size: 137 154 323 43 128 611 93 74
Cuts at: 1811 2001 2063 2793 248
Size: 190 62 730 1474
Fragments arranged by size:
1474 730 611 323 190 154 137 128
93 74 62 43
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 439 1069 1283 1585 1966 439
Size: 630 214 302 381 2492
Fragments arranged by size:
2492 630 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 163 447 2017 2198 2407 163
Size: 284 1570 181 209 1775
Fragments arranged by size:
1775 1570 284 209 181
BssSI C'ACGA_G
Cuts at: 906 2290 2648 906
Size: 1384 358 2277
Fragments arranged by size:
2277 1384 358
BstAPI GCAn_nnn'nTGC
Cuts at: 658 956 1396 658
Size: 298 440 3281
Fragments arranged by size:
3281 440 298
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstYI r'GATC_y
Cuts at: 1 254 1044 1511 1534 1869 2115 3127 3213
Size: 253 790 467 23 335 246 1012 86
Cuts at: 3213 3225 3571 1
Size: 12 346 449
Fragments arranged by size:
1012 790 467 449 346 335 253 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 356 578 107
Size: 249 222 3548
Fragments arranged by size:
3548 249 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 911 1969 2150 2313 911
Size: 1058 181 163 2617
Fragments arranged by size:
2617 1058 181 163
BtsI GCAGTG_nn'
Cuts at: 395 509 954 1262 395
Size: 114 445 308 3152
Fragments arranged by size:
3152 445 308 114
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 365 449 608 1604 1778 2169 2196 3541 365
Size: 84 159 996 174 391 27 1345 843
Fragments arranged by size:
1345 996 843 391 174 159 84 27
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 373 462 748 997 2042 2252 373
Size: 89 286 249 1045 210 2140
Fragments arranged by size:
2140 1045 286 249 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 383 537 903 1031 383
Size: 154 366 128 3371
Fragments arranged by size:
3371 366 154 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 427 752 1844 2276 3023 3291 211
Size: 216 325 1092 432 747 268 939
Fragments arranged by size:
1092 939 747 432 325 268 216
EcoO109I rG'GnC_Cy
Cuts at: 1158 1158
Size: 4019
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 1453 1701 2723 3702 1453
Size: 248 1022 979 1770
Fragments arranged by size:
1770 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1861 1891 1893 1923 1861
Size: 30 2 30 3957
Fragments arranged by size:
3957 30 30 2
HincII GTy'rAC
Cuts at: 483 1058 483
Size: 575 3444
HindIII A'AGCT_T
Cuts at: 324 324
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2690 2874 2690
Size: 184 3835
MscI TGG'CCA
Cuts at: 610 1780 610
Size: 1170 2849
MslI CAynn'nnrTG
Cuts at: 112 839 929 2008 2135 112
Size: 727 90 1079 127 1996
Fragments arranged by size:
1996 1079 727 127 90
MspA1I CmG'CkG
Cuts at: 658 767 1130 1444 1804 2817 3062 658
Size: 109 363 314 360 1013 245 1615
Fragments arranged by size:
1615 1013 363 360 314 245 109
NaeI GCC'GGC
Cuts at: 449 2200 449
Size: 1751 2268
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NcoI C'CATG_G
Cuts at: 107 107
Size: 4019
NgoMIV G'CCGG_C
Cuts at: 447 2198 447
Size: 1751 2268
NspI r_CATG'y
Cuts at: 215 740 1072 1572 2479 215
Size: 525 332 500 907 1755
Fragments arranged by size:
1755 907 525 500 332
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PfoI T'CCnGG_A
Cuts at: 137 137
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PsiI TTA'TAA
Cuts at: 854 854
Size: 4019
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 218 250 218
Size: 32 3987
PstI C_TGCA'G
Cuts at: 663 1066 1751 663
Size: 403 685 2931
Fragments arranged by size:
2931 685 403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 658 1444 1804 658
Size: 786 360 2873
Fragments arranged by size:
2873 786 360
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 385 539 905 1033 385
Size: 154 366 128 3371
Fragments arranged by size:
3371 366 154 128
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 373 462 2042 2252 373
Size: 89 1580 210 2140
Fragments arranged by size:
2140 1580 210 89
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 235 659 915 1062 1747 2740 2931 33
Size: 202 424 256 147 685 993 191 1121
Fragments arranged by size:
1121 993 685 424 256 202 191 147
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 1174 2089 2581 2843 1174
Size: 915 492 262 2350
Fragments arranged by size:
2350 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
StyI C'CwwG_G
Cuts at: 107 714 1153 1507 107
Size: 607 439 354 2619
Fragments arranged by size:
2619 607 439 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1602 1735 2194 2403 1602
Size: 133 459 209 3218
Fragments arranged by size:
3218 459 209 133
TatI w'GTAC_w
Cuts at: 3534 3534
Size: 4019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 1816 1816
Size: 4019
XbaI T'CTAG_A
Cuts at: 462 1050 462
Size: 588 3431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1514 114
Size: 1400 2619
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI