Restriction map for
pF1KB8285
(Circular) (Six-base) MAPSORT of: pF1KB8285.seq Check: 7961 from: 1 to: 4295
pF1KB8285 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 888 2120 2552 3299 3567 578
Size: 310 1232 432 747 268 1306
Fragments arranged by size:
1306 1232 747 432 310 268
AflIII A'CryG_T
Cuts at: 1647 2751 3728 1647
Size: 1104 977 2214
Fragments arranged by size:
2214 1104 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AleI CACnn'nnGTG
Cuts at: 481 551 601 481
Size: 70 50 4175
Fragments arranged by size:
4175 70 50
AlwNI CAG_nnn'CTG
Cuts at: 1600 3167 1600
Size: 1567 2728
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 757 1048 1066 1299 3648 3904 757
Size: 291 18 233 2349 256 1148
Fragments arranged by size:
2349 1148 291 256 233 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 190 223 190
Size: 33 4262
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 1311 1725 1973 2008 1311
Size: 414 248 35 3598
Fragments arranged by size:
3598 414 248 35
BanII G_rGCy'C
Cuts at: 1309 2339 1309
Size: 1030 3265
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbvCI CC'TCA_GC
Cuts at: 146 146
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3069 1920
Size: 93 1056 3146
Fragments arranged by size:
3146 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1053 1507 1588 1923 4067 1053
Size: 454 81 335 2144 1281
Fragments arranged by size:
2144 1281 454 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1165
Size: 4295
Bpu10I CC'TnA_GC
Cuts at: 146 228 497 801 146
Size: 82 269 304 3640
Fragments arranged by size:
3640 304 269 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1471 2350 2842 3140 391
Size: 1080 879 492 298 1546
Fragments arranged by size:
1546 1080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 848 3999 848
Size: 3151 1144
BsaAI yAC'GTr
Cuts at: 895 1648 895
Size: 753 3542
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1168 1168
Size: 4295
BseYI C'CCAG_C
Cuts at: 1282 1720 2478 3055 3551 1282
Size: 438 758 577 496 2026
Fragments arranged by size:
2026 758 577 496 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 627
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3069 1309
Size: 778 190 792 2535
Fragments arranged by size:
2535 792 778 190
BsmI GAATG_Cn'
Cuts at: 1251 1251
Size: 4295
Bsp1286I G_dGCh'C
Cuts at: 1309 1920 2013 2087 2277 2339 3069 1309
Size: 611 93 74 190 62 730 2535
Fragments arranged by size:
2535 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1559 1861 2242 1345
Size: 214 302 381 3398
Fragments arranged by size:
3398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 2293 2474 2683 2293
Size: 181 209 3905
Fragments arranged by size:
3905 209 181
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1672 1246
Size: 426 3869
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 276 451 276
Size: 175 4120
BstYI r'GATC_y
Cuts at: 1 1320 1787 1810 2145 2391 3403 3489 3501
Size: 1319 467 23 335 246 1012 86 12
Cuts at: 3501 3847 1
Size: 346 449
Fragments arranged by size:
1319 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 171 444 2245 2426 2589 171
Size: 273 1801 181 163 1877
Fragments arranged by size:
1877 1801 273 181 163
BtsI GCAGTG_nn'
Cuts at: 1538 1538
Size: 4295
ClaI AT'CG_AT
Cuts at: 692 692
Size: 4295
DraI TTT'AAA
Cuts at: 1065 1294 1065
Size: 229 4066
DrdI GACnn_nn'nnGTC
Cuts at: 439 2001 2859 439
Size: 1562 858 1875
Fragments arranged by size:
1875 1562 858
EaeI y'GGCC_r
Cuts at: 1880 2054 2445 2472 3817 1880
Size: 174 391 27 1345 2358
Fragments arranged by size:
2358 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 379 2318 2528 379
Size: 1939 210 2146
Fragments arranged by size:
2146 1939 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 2813 2959 4105 2503
Size: 310 146 1146 2693
Fragments arranged by size:
2693 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 888 1165 2120 2552 3299 3567 578
Size: 310 277 955 432 747 268 1306
Fragments arranged by size:
1306 955 747 432 310 277 268
EcoO109I rG'GnC_Cy
Cuts at: 150 503 1173 1434 150
Size: 353 670 261 3011
Fragments arranged by size:
3011 670 353 261
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 1729 1977 2999 3978 1729
Size: 248 1022 979 2046
Fragments arranged by size:
2046 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2137 2167 2169 2199 2137
Size: 30 2 30 4233
Fragments arranged by size:
4233 30 30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 2056 2056
Size: 4295
MslI CAynn'nnrTG
Cuts at: 274 449 481 551 601 2284 2411 274
Size: 175 32 70 50 1683 127 2158
Fragments arranged by size:
2158 1683 175 127 70 50 32
MspA1I CmG'CkG
Cuts at: 1406 1720 2080 3093 3338 1406
Size: 314 360 1013 245 2363
Fragments arranged by size:
2363 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 688 1348 1848 2755 688
Size: 660 500 907 2228
Fragments arranged by size:
2228 907 660 500
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PfoI T'CCnGG_A
Cuts at: 1154 1154
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PmlI CAC'GTG
Cuts at: 895 895
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 150 503 150
Size: 353 3942
PstI C_TGCA'G
Cuts at: 1242 1342 2027 1242
Size: 100 685 3510
Fragments arranged by size:
3510 685 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1720 2080 1720
Size: 360 3935
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SanDI GG'GwC_CC
Cuts at: 503 503
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 379 2318 2528 379
Size: 1939 210 2146
Fragments arranged by size:
2146 1939 210
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SexAI A'CCwGG_T
Cuts at: 152 152
Size: 4295
SfcI C'TryA_G
Cuts at: 33 1238 1338 2023 3016 3207 33
Size: 1205 100 685 993 191 1121
Fragments arranged by size:
1205 1121 993 685 191 100
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 406 1450 2365 2857 3119 406
Size: 1044 915 492 262 1582
Fragments arranged by size:
1582 1044 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 1228 1228
Size: 4295
StyI C'CwwG_G
Cuts at: 209 435 818 1429 1783 209
Size: 226 383 611 354 2721
Fragments arranged by size:
2721 611 383 354 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 970 1878 2011 2470 2679 194
Size: 776 908 133 459 209 1810
Fragments arranged by size:
1810 908 776 459 209 133
TatI w'GTAC_w
Cuts at: 3810 3810
Size: 4295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1095 1127 3812 3844 1095
Size: 32 2685 32 1546
Fragments arranged by size:
2685 1546 32 32
Tth111I GACn'n_nGTC
Cuts at: 154 2092 154
Size: 1938 2357
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 1790 171
Size: 1619 2676
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 295 295
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI