Restriction map for
pF1KB8283
(Circular) (Six-base) MAPSORT of: pF1KB8283.seq Check: 261 from: 1 to: 3989
pF1KB8283 3989 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 501 1814 2246 2993 3261 165
Size: 336 1313 432 747 268 893
Fragments arranged by size:
1313 893 747 432 336 268
AflIII A'CryG_T
Cuts at: 711 1341 2445 3422 711
Size: 630 1104 977 1278
Fragments arranged by size:
1278 1104 977 630
AgeI A'CCGG_T
Cuts at: 2377 2377
Size: 3989
AlwNI CAG_nnn'CTG
Cuts at: 1294 2861 1294
Size: 1567 2422
ApaLI G'TGCA_C
Cuts at: 2759 2759
Size: 3989
ApoI r'AATT_y
Cuts at: 131 383 971 993 3342 3598 131
Size: 252 588 22 2349 256 522
Fragments arranged by size:
2349 588 522 256 252 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 1009 1009
Size: 3989
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 1005 1419 1667 1702 1005
Size: 414 248 35 3292
Fragments arranged by size:
3292 414 248 35
BanII G_rGCy'C
Cuts at: 781 1003 2033 781
Size: 222 1030 2737
Fragments arranged by size:
2737 1030 222
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BbsI GAAGACnn'nnnn_
Cuts at: 171 171
Size: 3989
BbvCI CC'TCA_GC
Cuts at: 118 118
Size: 3989
BciVI GTATCCnnnnn_n'
Cuts at: 1887 2648 3989 1887
Size: 761 1341 1887
Fragments arranged by size:
1887 1341 761
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglII A'GATC_T
Cuts at: 1504 3541 1504
Size: 2037 1952
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 1614 1707 2763 1614
Size: 93 1056 2840
Fragments arranged by size:
2840 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1201 1282 1617 3761 1201
Size: 81 335 2144 1429
Fragments arranged by size:
2144 1429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 800 2356 800
Size: 1556 2433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 665 413
Size: 252 3737
Bpu10I CC'TnA_GC
Cuts at: 118 118
Size: 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 2044 2536 2834 1165
Size: 879 492 298 2320
Fragments arranged by size:
2320 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3693 3693
Size: 3989
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 1503 3540 6
Size: 1497 2037 455
Fragments arranged by size:
2037 1497 455
BsaHI Gr'CG_yC
Cuts at: 1420 1668 2370 3866 1420
Size: 248 702 1496 1543
Fragments arranged by size:
1543 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1178 1699 2377 2651 2798 3209 1178
Size: 521 678 274 147 411 1958
Fragments arranged by size:
1958 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1414 2172 2749 3245 1414
Size: 758 577 496 2158
Fragments arranged by size:
2158 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 936 936
Size: 3989
BsiEI CG_ry'CG
Cuts at: 104 1531 1577 2785 104
Size: 1427 46 1208 1308
Fragments arranged by size:
1427 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 781 1003 1781 1971 2763 781
Size: 222 778 190 792 2007
Fragments arranged by size:
2007 792 778 222 190
BsmI GAATG_Cn'
Cuts at: 751 751
Size: 3989
Bsp1286I G_dGCh'C
Cuts at: 781 1003 1614 1707 1781 1971 2033 2763 781
Size: 222 611 93 74 190 62 730 2007
Fragments arranged by size:
2007 730 611 222 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3209 3209
Size: 3989
BspHI T'CATG_A
Cuts at: 3165 3165
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1039 1253 1555 1936 1039
Size: 214 302 381 3092
Fragments arranged by size:
3092 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2281 2335 3819 3984 50
Size: 2231 54 1484 165 55
Fragments arranged by size:
2231 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 756 1901 756
Size: 1145 2844
BsrFI r'CCGG_y
Cuts at: 1987 2168 2377 1987
Size: 181 209 3599
Fragments arranged by size:
3599 209 181
BsrGI T'GTAC_A
Cuts at: 708 708
Size: 3989
BssSI C'ACGA_G
Cuts at: 222 2260 2618 222
Size: 2038 358 1593
Fragments arranged by size:
2038 1593 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1366 1366
Size: 3989
BstBI TT'CG_AA
Cuts at: 323 2350 323
Size: 2027 1962
BstYI r'GATC_y
Cuts at: 1 1014 1481 1504 1839 2085 3097 3183 3195
Size: 1013 467 23 335 246 1012 86 12
Cuts at: 3195 3541 1
Size: 346 449
Fragments arranged by size:
1013 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 924 1232 924
Size: 308 3681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 738 773 738
Size: 35 3954
DraI TTT'AAA
Cuts at: 576 988 576
Size: 412 3577
DrdI GACnn_nn'nnGTC
Cuts at: 1695 2553 1695
Size: 858 3131
EaeI y'GGCC_r
Cuts at: 603 1574 1748 2139 2166 3511 603
Size: 971 174 391 27 1345 1081
Fragments arranged by size:
1345 1081 971 391 174 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2197 2507 2653 3799 2197
Size: 310 146 1146 2387
Fragments arranged by size:
2387 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 1001 779
Size: 222 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 413 501 665 1814 2246 2993 3261 165
Size: 248 88 164 1149 432 747 268 893
Fragments arranged by size:
1149 893 747 432 268 248 164 88
EcoO109I rG'GnC_Cy
Cuts at: 1128 1128
Size: 3989
EcoRI G'AATT_C
Cuts at: 993 993
Size: 3989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 1423 1671 2693 3672 1423
Size: 248 1022 979 1740
Fragments arranged by size:
1740 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1831 1861 1863 1893 1831
Size: 30 2 30 3927
Fragments arranged by size:
3927 30 30 2
HincII GTy'rAC
Cuts at: 1028 1028
Size: 3989
HindIII A'AGCT_T
Cuts at: 316 428 848 932 316
Size: 112 420 84 3373
Fragments arranged by size:
3373 420 112 84
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 2660 2844 554
Size: 2106 184 1699
Fragments arranged by size:
2106 1699 184
MscI TGG'CCA
Cuts at: 605 1750 605
Size: 1145 2844
MslI CAynn'nnrTG
Cuts at: 728 1978 2105 728
Size: 1250 127 2612
Fragments arranged by size:
2612 1250 127
MspA1I CmG'CkG
Cuts at: 1100 1414 1774 2787 3032 1100
Size: 314 360 1013 245 2057
Fragments arranged by size:
2057 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 3989
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 3989
NspI r_CATG'y
Cuts at: 715 799 1042 1542 2449 715
Size: 84 243 500 907 2255
Fragments arranged by size:
2255 907 500 243 84
PciI A'CATG_T
Cuts at: 711 2445 711
Size: 1734 2255
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3957
PstI C_TGCA'G
Cuts at: 1036 1721 1036
Size: 685 3304
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 1414 1774 1414
Size: 360 3629
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 781 1003 781
Size: 222 3767
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 1036 1036
Size: 3989
ScaI AGT'ACT
Cuts at: 3506 3506
Size: 3989
SfcI C'TryA_G
Cuts at: 33 1032 1717 2710 2901 33
Size: 999 685 993 191 1121
Fragments arranged by size:
1121 999 993 685 191
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 1011 1011
Size: 3989
SmlI C'TyrA_G
Cuts at: 1144 2059 2551 2813 1144
Size: 915 492 262 2320
Fragments arranged by size:
2320 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 1042 1542 1042
Size: 500 3489
StyI C'CwwG_G
Cuts at: 1123 1477 1123
Size: 354 3635
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 1572 1705 2164 2373 316
Size: 1256 133 459 209 1932
Fragments arranged by size:
1932 1256 459 209 133
TatI w'GTAC_w
Cuts at: 708 3504 708
Size: 2796 1193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 3957
Tth111I GACn'n_nGTC
Cuts at: 1786 1786
Size: 3989
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1484 1484
Size: 3989
XmaI C'CCGG_G
Cuts at: 1009 1009
Size: 3989
XmnI GAAnn'nnTTC
Cuts at: 319 430 850 934 319
Size: 111 420 84 3374
Fragments arranged by size:
3374 420 111 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI