Restriction map for
pF1KB8282
(Circular) (Six-base) MAPSORT of: pF1KB8282.seq Check: 2789 from: 1 to: 4037
pF1KB8282 4037 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 140 140
Size: 4037
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 647 1862 2294 3041 3309 496
Size: 151 1215 432 747 268 1224
Fragments arranged by size:
1224 1215 747 432 268 151
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 1342 2909 1342
Size: 1567 2470
ApaI G_GGCC'C
Cuts at: 941 941
Size: 4037
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 526 1057 526
Size: 531 3506
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 823 1053 1467 1715 1750 823
Size: 230 414 248 35 3110
Fragments arranged by size:
3110 414 248 230 35
BanII G_rGCy'C
Cuts at: 941 1051 2081 941
Size: 110 1030 2897
Fragments arranged by size:
2897 1030 110
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BbsI GAAGACnn'nnnn_
Cuts at: 653 781 653
Size: 128 3909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4003
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 505 941 1662 1755 2811 505
Size: 436 721 93 1056 1731
Fragments arranged by size:
1731 1056 721 436 93
BmgBI CAC'GTC
Cuts at: 210 269 210
Size: 59 3978
BmrI ACTGGGnnnn_n'
Cuts at: 1249 1330 1665 3809 1249
Size: 81 335 2144 1477
Fragments arranged by size:
2144 1477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1213 2092 2584 2882 802
Size: 411 879 492 298 1957
Fragments arranged by size:
1957 879 492 411 298
BsaI GGTCTCn'nnnn_
Cuts at: 993 3741 993
Size: 2748 1289
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 137 1468 1716 2418 3914 137
Size: 1331 248 702 1496 260
Fragments arranged by size:
1496 1331 702 260 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 113 143 113
Size: 30 4007
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 186 940 183
Size: 3 754 3280
Fragments arranged by size:
3280 754 3
BseYI C'CCAG_C
Cuts at: 1462 2220 2797 3293 1462
Size: 758 577 496 2206
Fragments arranged by size:
2206 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 638 1051 1829 2019 2811 638
Size: 413 778 190 792 1864
Fragments arranged by size:
1864 792 778 413 190
BsmI GAATG_Cn'
Cuts at: 733 733
Size: 4037
BsmBI CGTCTCn'nnnn_
Cuts at: 847 847
Size: 4037
Bsp1286I G_dGCh'C
Cuts at: 505 638 941 1051 1662 1755 1829 2019 2081
Size: 133 303 110 611 93 74 190 62
Cuts at: 2081 2811 505
Size: 730 1731
Fragments arranged by size:
1731 730 611 303 190 133 110 93
74 62
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 263 3213 263
Size: 2950 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 897 2329 2383 3867 4032 50
Size: 847 1432 54 1484 165 55
Fragments arranged by size:
1484 1432 847 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BssHII G'CGCG_C
Cuts at: 531 531
Size: 4037
BssSI C'ACGA_G
Cuts at: 996 2308 2666 996
Size: 1312 358 2367
Fragments arranged by size:
2367 1312 358
BstAPI GCAn_nnn'nTGC
Cuts at: 551 1414 551
Size: 863 3174
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstYI r'GATC_y
Cuts at: 1 1062 1529 1552 1887 2133 3145 3231 3243
Size: 1061 467 23 335 246 1012 86 12
Cuts at: 3243 3589 1
Size: 346 449
Fragments arranged by size:
1061 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 589 589
Size: 4037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 921 1987 2168 2331 921
Size: 1066 181 163 2627
Fragments arranged by size:
2627 1066 181 163
BtsI GCAGTG_nn'
Cuts at: 540 582 724 738 806 1280 540
Size: 42 142 14 68 474 3297
Fragments arranged by size:
3297 474 142 68 42 14
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 374 1622 1796 2187 2214 3559 374
Size: 1248 174 391 27 1345 852
Fragments arranged by size:
1345 1248 852 391 174 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 186 643 2060 2270 186
Size: 457 1417 210 1953
Fragments arranged by size:
1953 1417 457 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 647 1862 2294 3041 3309 496
Size: 151 1215 432 747 268 1224
Fragments arranged by size:
1224 1215 747 432 268 151
EcoO109I rG'GnC_Cy
Cuts at: 937 938 1176 937
Size: 1 238 3798
Fragments arranged by size:
3798 238 1
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 945 2056 945
Size: 1111 2926
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 1471 1719 2741 3720 1471
Size: 248 1022 979 1788
Fragments arranged by size:
1788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 169 201 1879 1909 1911 1941 113
Size: 32 24 32 1678 30 2 30 2209
Fragments arranged by size:
2209 1678 32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 351 1076 351
Size: 725 3312
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MfeI C'AATT_G
Cuts at: 600 600
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2708 2892 2708
Size: 184 3853
MscI TGG'CCA
Cuts at: 1798 1798
Size: 4037
MslI CAynn'nnrTG
Cuts at: 2026 2153 2026
Size: 127 3910
MspA1I CmG'CkG
Cuts at: 474 1148 1462 1822 2835 3080 474
Size: 674 314 360 1013 245 1431
Fragments arranged by size:
1431 1013 674 360 314 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NdeI CA'TA_TG
Cuts at: 841 841
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 1090 1590 2497 1090
Size: 500 907 2630
Fragments arranged by size:
2630 907 500
PasI CC'CwG_GG
Cuts at: 421 421
Size: 4037
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PfoI T'CCnGG_A
Cuts at: 131 131
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PshAI GACnn'nnGTC
Cuts at: 307 307
Size: 4037
PspOMI G'GGCC_C
Cuts at: 937 937
Size: 4037
PstI C_TGCA'G
Cuts at: 547 813 1084 1769 547
Size: 266 271 685 2815
Fragments arranged by size:
2815 685 271 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 474 1462 1822 474
Size: 988 360 2689
Fragments arranged by size:
2689 988 360
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 643 2060 2270 643
Size: 1417 210 2410
Fragments arranged by size:
2410 1417 210
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 543 809 1080 1765 2758 2949 33
Size: 510 266 271 685 993 191 1121
Fragments arranged by size:
1121 993 685 510 271 266 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 781 1192 2107 2599 2861 781
Size: 411 915 492 262 1957
Fragments arranged by size:
1957 915 492 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StyI C'CwwG_G
Cuts at: 1171 1525 1171
Size: 354 3683
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 582 841 1620 1753 2212 2421 582
Size: 259 779 133 459 209 2198
Fragments arranged by size:
2198 779 459 259 209 133
TatI w'GTAC_w
Cuts at: 3552 3552
Size: 4037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 220 1834 220
Size: 1614 2423
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
ZraI GAC'GTC
Cuts at: 138 138
Size: 4037
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I
BsgI BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI