Restriction map for
pF1KB8281
(Circular) (Six-base) MAPSORT of: pF1KB8281.seq Check: 7953 from: 1 to: 4028
pF1KB8281 4028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1853 2285 3032 3300 787
Size: 1066 432 747 268 1515
Fragments arranged by size:
1515 1066 747 432 268
AfeI AGC'GCT
Cuts at: 309 309
Size: 4028
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AleI CACnn'nnGTG
Cuts at: 592 817 592
Size: 225 3803
AlwNI CAG_nnn'CTG
Cuts at: 1333 2900 1333
Size: 1567 2461
ApaLI G'TGCA_C
Cuts at: 595 2798 595
Size: 2203 1825
ApoI r'AATT_y
Cuts at: 1032 3381 3637 1032
Size: 2349 256 1423
Fragments arranged by size:
2349 1423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 936 975 1019 1048 936
Size: 39 44 29 3916
Fragments arranged by size:
3916 44 39 29
AvrII C'CTAG_G
Cuts at: 835 835
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 183 574 1042 2072 183
Size: 391 468 1030 2139
Fragments arranged by size:
2139 1030 468 391
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbvCI CC'TCA_GC
Cuts at: 408 408
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BfrBI ATG'CAT
Cuts at: 699 699
Size: 4028
BglI GCCn_nnn'nGGC
Cuts at: 760 823 894 760
Size: 63 71 3894
Fragments arranged by size:
3894 71 63
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 599 1653 1746 2802 599
Size: 1054 93 1056 1825
Fragments arranged by size:
1825 1056 1054 93
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 593 2395 593
Size: 1802 2226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 381 417 435 987 351
Size: 30 36 18 552 3392
Fragments arranged by size:
3392 552 36 30 18
Bpu10I CC'TnA_GC
Cuts at: 360 408 423 581 360
Size: 48 15 158 3807
Fragments arranged by size:
3807 158 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 1204 2083 2575 2873 369
Size: 835 879 492 298 1524
Fragments arranged by size:
1524 879 835 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 341 908 3732 341
Size: 567 2824 637
Fragments arranged by size:
2824 637 567
BsaAI yAC'GTr
Cuts at: 729 1381 729
Size: 652 3376
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 165 195 165
Size: 30 3998
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 933 1005 933
Size: 72 3956
BseYI C'CCAG_C
Cuts at: 1453 2211 2788 3284 1453
Size: 758 577 496 2197
Fragments arranged by size:
2197 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 282 1570 1616 2824 104
Size: 178 1288 46 1208 1308
Fragments arranged by size:
1308 1288 1208 178 46
BsiHKAI G_wGCw'C
Cuts at: 599 1042 1820 2010 2802 599
Size: 443 778 190 792 1825
Fragments arranged by size:
1825 792 778 443 190
BsiWI C'GTAC_G
Cuts at: 725 725
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 183 574 599 1042 1653 1746 1820 2010 2072
Size: 391 25 443 611 93 74 190 62
Cuts at: 2072 2802 183
Size: 730 1409
Fragments arranged by size:
1409 730 611 443 391 190 93 74
62 25
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 890 935 1078 1292 1594 1975 890
Size: 45 143 214 302 381 2943
Fragments arranged by size:
2943 381 302 214 143 45
BsrBI CCG'CTC
Cuts at: 50 209 422 521 932 1001 2320 2374 3858
Size: 159 213 99 411 69 1319 54 1484
Cuts at: 3858 4023 50
Size: 165 55
Fragments arranged by size:
1484 1319 411 213 165 159 99 69
55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstYI r'GATC_y
Cuts at: 1 1053 1520 1543 1878 2124 3136 3222 3234
Size: 1052 467 23 335 246 1012 86 12
Cuts at: 3234 3580 1
Size: 346 449
Fragments arranged by size:
1052 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 686 910 107
Size: 579 224 3225
Fragments arranged by size:
3225 579 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 396 806 1271 396
Size: 410 465 3153
Fragments arranged by size:
3153 465 410
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 279 795 1613 1787 2178 2205 3550 279
Size: 516 818 174 391 27 1345 757
Fragments arranged by size:
1345 818 757 516 391 174 27
EagI C'GGCC_G
Cuts at: 279 1613 279
Size: 1334 2694
EarI CTCTTCn'nnn_
Cuts at: 127 408 471 2051 2261 127
Size: 281 63 1580 210 1894
Fragments arranged by size:
1894 1580 281 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 582 2236 2546 2692 3838 582
Size: 1654 310 146 1146 772
Fragments arranged by size:
1654 1146 772 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 381 417 435 787 987 1853 2285 3032
Size: 30 36 18 352 200 866 432 747
Cuts at: 3032 3300 351
Size: 268 1079
Fragments arranged by size:
1079 866 747 432 352 268 200 36
30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 834 834
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 926 947 1167 926
Size: 21 220 3787
Fragments arranged by size:
3787 220 21
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 153 2047 153
Size: 1894 2134
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 311 516 766 1462 1710 2732 3711 311
Size: 205 250 696 248 1022 979 628
Fragments arranged by size:
1022 979 696 628 250 248 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 615 647 1870 1900 1902 1932 205
Size: 32 378 32 1223 30 2 30 2301
Fragments arranged by size:
2301 1223 378 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1034 2699 2883 1034
Size: 1665 184 2179
Fragments arranged by size:
2179 1665 184
MscI TGG'CCA
Cuts at: 797 1789 797
Size: 992 3036
MslI CAynn'nnrTG
Cuts at: 592 685 776 817 2017 2144 592
Size: 93 91 41 1200 127 2476
Fragments arranged by size:
2476 1200 127 93 91 41
MspA1I CmG'CkG
Cuts at: 1139 1453 1813 2826 3071 1139
Size: 314 360 1013 245 2096
Fragments arranged by size:
2096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 107
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NsiI A_TGCA'T
Cuts at: 701 701
Size: 4028
NspI r_CATG'y
Cuts at: 684 1081 1581 2488 684
Size: 397 500 907 2224
Fragments arranged by size:
2224 907 500 397
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PpuMI rG'GwC_Cy
Cuts at: 926 947 926
Size: 21 4007
PstI C_TGCA'G
Cuts at: 1075 1760 1075
Size: 685 3343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 127 408 471 2051 2261 127
Size: 281 63 1580 210 1894
Fragments arranged by size:
1894 1580 281 210 63
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SexAI A'CCwGG_T
Cuts at: 331 331
Size: 4028
SfcI C'TryA_G
Cuts at: 33 1071 1756 2749 2940 33
Size: 1038 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 191
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 977 1050 977
Size: 73 3955
SmlI C'TyrA_G
Cuts at: 384 1183 2098 2590 2852 384
Size: 799 915 492 262 1560
Fragments arranged by size:
1560 915 799 492 262
SnaBI TAC'GTA
Cuts at: 729 729
Size: 4028
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 107 224 254 835 1162 1516 107
Size: 117 30 581 327 354 2619
Fragments arranged by size:
2619 581 354 327 117 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 329 1611 1744 2203 2412 329
Size: 1282 133 459 209 1945
Fragments arranged by size:
1945 1282 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 3545 3577 165
Size: 32 3348 32 616
Fragments arranged by size:
3348 616 32 32
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 819 890 1523 819
Size: 71 633 3324
Fragments arranged by size:
3324 633 71
XmaI C'CCGG_G
Cuts at: 975 1048 975
Size: 73 3955
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BcgI BmgBI BmtI BsgI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI