Restriction map for
pF1KB8279
(Circular) (Six-base) MAPSORT of: pF1KB8279.seq Check: 4735 from: 1 to: 4331
pF1KB8279 4331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 651 1369 651
Size: 718 3613
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 562 565 918 1002 2156 2588 3335 3603
Size: 26 3 353 84 1154 432 747 268
Cuts at: 3603 536
Size: 1264
Fragments arranged by size:
1264 1154 747 432 353 268 84 26
3
AfeI AGC'GCT
Cuts at: 1156 1156
Size: 4331
AflII C'TTAA_G
Cuts at: 136 1288 136
Size: 1152 3179
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AhdI GACnn_n'nnGTC
Cuts at: 260 260
Size: 4331
AleI CACnn'nnGTG
Cuts at: 1229 1229
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 1636 3203 1636
Size: 1567 2764
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 266 1351 266
Size: 1085 3246
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 1347 1761 2009 2044 1347
Size: 414 248 35 3634
Fragments arranged by size:
3634 414 248 35
BanII G_rGCy'C
Cuts at: 392 1345 2375 392
Size: 953 1030 2348
Fragments arranged by size:
2348 1030 953
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 164 164
Size: 4331
BbvCI CC'TCA_GC
Cuts at: 1108 1108
Size: 4331
BciVI GTATCCnnnnn_n'
Cuts at: 1130 2229 2990 4331 1130
Size: 1099 761 1341 1130
Fragments arranged by size:
1341 1130 1099 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglII A'GATC_T
Cuts at: 453 1846 3883 453
Size: 1393 2037 901
Fragments arranged by size:
2037 1393 901
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 1956 2049 3105 1956
Size: 93 1056 3182
Fragments arranged by size:
3182 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 902 1543 1624 1959 4103 902
Size: 641 81 335 2144 1130
Fragments arranged by size:
2144 1130 641 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 379 476 830 998 1166 1334 376
Size: 3 97 354 168 168 168 3373
Fragments arranged by size:
3373 354 168 168 168 97 3
Bpu10I CC'TnA_GC
Cuts at: 310 1108 310
Size: 798 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1314 4035 1314
Size: 2721 1610
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 606 1845 3882 6
Size: 600 1239 2037 455
Fragments arranged by size:
2037 1239 600 455
BsaHI Gr'CG_yC
Cuts at: 819 1762 2010 2712 4208 819
Size: 943 248 702 1496 942
Fragments arranged by size:
1496 943 942 702 248
BsaWI w'CCGG_w
Cuts at: 417 1520 2041 2719 2993 3140 3551 417
Size: 1103 521 678 274 147 411 1197
Fragments arranged by size:
1197 1103 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 445 1096 400
Size: 45 651 3635
Fragments arranged by size:
3635 651 45
BseYI C'CCAG_C
Cuts at: 1756 2514 3091 3587 1756
Size: 758 577 496 2500
Fragments arranged by size:
2500 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1269 1278 1269
Size: 9 4322
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 392 413 524 878 1345 2123 2313 3105 392
Size: 21 111 354 467 778 190 792 1618
Fragments arranged by size:
1618 792 778 467 354 190 111 21
BsmI GAATG_Cn'
Cuts at: 1084 1200 1084
Size: 116 4215
BsmBI CGTCTCn'nnnn_
Cuts at: 810 810
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 392 413 524 878 1345 1956 2049 2123 2313
Size: 21 111 354 467 611 93 74 190
Cuts at: 2313 2375 3105 392
Size: 62 730 1618
Fragments arranged by size:
1618 730 611 467 354 190 111 93
74 62 21
BspEI T'CCGG_A
Cuts at: 417 3551 417
Size: 3134 1197
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 1595 1897 2278 1381
Size: 214 302 381 3434
Fragments arranged by size:
3434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 837 1035 2243 837
Size: 198 1208 2925
Fragments arranged by size:
2925 1208 198
BsrFI r'CCGG_y
Cuts at: 281 2329 2510 2719 281
Size: 2048 181 209 1893
Fragments arranged by size:
2048 1893 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1256 1708 1256
Size: 452 3879
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 505 505
Size: 4331
BstYI r'GATC_y
Cuts at: 1 453 1356 1823 1846 2181 2427 3439 3525
Size: 452 903 467 23 335 246 1012 86
Cuts at: 3525 3537 3883 1
Size: 12 346 449
Fragments arranged by size:
1012 903 467 452 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 652 652
Size: 4331
Bsu36I CC'TnA_GG
Cuts at: 383 383
Size: 4331
BtgI C'CryG_G
Cuts at: 107 107
Size: 4331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1574 1574
Size: 4331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 819 854 1071 1106 819
Size: 35 217 35 4044
Fragments arranged by size:
4044 217 35 35
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 207 1916 2090 2481 2508 3853 207
Size: 1709 174 391 27 1345 685
Fragments arranged by size:
1709 1345 685 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 981 2539 2849 2995 4141 981
Size: 1558 310 146 1146 1171
Fragments arranged by size:
1558 1171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 390 1343 390
Size: 953 3378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 379 476 536 562 565 830 918 998
Size: 3 97 60 26 3 265 88 80
Cuts at: 998 1002 1166 1334 2156 2588 3335 3603 376
Size: 4 164 168 822 432 747 268 1104
Fragments arranged by size:
1104 822 747 432 268 265 168 164
97 88 80 60 26 4 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 382 382
Size: 4331
EcoO109I rG'GnC_Cy
Cuts at: 1470 1470
Size: 4331
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1158 1765 2013 3035 4014 1158
Size: 607 248 1022 979 1475
Fragments arranged by size:
1475 1022 979 607 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 278 658 690 2173 2203 2205 2235 246
Size: 32 380 32 1483 30 2 30 2342
Fragments arranged by size:
2342 1483 380 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
HindIII A'AGCT_T
Cuts at: 846 846
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 209 2092 209
Size: 1883 2448
MslI CAynn'nnrTG
Cuts at: 503 909 1077 1229 2320 2447 503
Size: 406 168 152 1091 127 2387
Fragments arranged by size:
2387 1091 406 168 152 127
MspA1I CmG'CkG
Cuts at: 1442 1756 2116 3129 3374 1442
Size: 314 360 1013 245 2399
Fragments arranged by size:
2399 1013 360 314 245
NaeI GCC'GGC
Cuts at: 283 2512 283
Size: 2229 2102
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 107
Size: 4331
NdeI CA'TA_TG
Cuts at: 906 1074 906
Size: 168 4163
NgoMIV G'CCGG_C
Cuts at: 281 2510 281
Size: 2229 2102
NspI r_CATG'y
Cuts at: 1384 1884 2791 1384
Size: 500 907 2924
Fragments arranged by size:
2924 907 500
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 669 669
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 392 1345 392
Size: 953 3378
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 866 3848 866
Size: 2982 1349
SfcI C'TryA_G
Cuts at: 33 443 1374 2059 3052 3243 33
Size: 410 931 685 993 191 1121
Fragments arranged by size:
1121 993 931 685 410 191
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 136 1288 1486 2401 2893 3155 136
Size: 1152 198 915 492 262 1312
Fragments arranged by size:
1312 1152 915 492 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
StuI AGG'CCT
Cuts at: 397 931 397
Size: 534 3797
StyI C'CwwG_G
Cuts at: 107 1465 1819 107
Size: 1358 354 2619
Fragments arranged by size:
2619 1358 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 1914 2047 2506 2715 390
Size: 1524 133 459 209 2006
Fragments arranged by size:
2006 1524 459 209 133
TatI w'GTAC_w
Cuts at: 752 864 3846 752
Size: 112 2982 1237
Fragments arranged by size:
2982 1237 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 256 421 1826 256
Size: 165 1405 2761
Fragments arranged by size:
2761 1405 165
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
XmnI GAAnn'nnTTC
Cuts at: 847 931 1015 1099 847
Size: 84 84 84 4079
Fragments arranged by size:
4079 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AlfI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsrGI
BssHII BstEII ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI