Restriction map for
pF1KB8274
(Circular) (Six-base) MAPSORT of: pF1KB8274.seq Check: 4297 from: 1 to: 3419
pF1KB8274 3419 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 457 457
Size: 3419
Acc65I G'GTAC_C
Cuts at: 435 435
Size: 3419
AclI AA'CG_TT
Cuts at: 3259 3259
Size: 3419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 364 1244 1676 2423 2691 178
Size: 186 880 432 747 268 906
Fragments arranged by size:
906 880 747 432 268 186
AflIII A'CryG_T
Cuts at: 771 1875 2852 771
Size: 1104 977 1338
Fragments arranged by size:
1338 1104 977
AgeI A'CCGG_T
Cuts at: 1807 1807
Size: 3419
AlwNI CAG_nnn'CTG
Cuts at: 724 2291 724
Size: 1567 1852
ApaI G_GGCC'C
Cuts at: 338 338
Size: 3419
ApaLI G'TGCA_C
Cuts at: 2189 2189
Size: 3419
ApoI r'AATT_y
Cuts at: 423 2772 3028 423
Size: 2349 256 814
Fragments arranged by size:
2349 814 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3419
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3419
AvaI C'yCGr_G
Cuts at: 439 439
Size: 3419
BamHI G'GATC_C
Cuts at: 375 444 375
Size: 69 3350
BanI G'GyrC_C
Cuts at: 435 849 1097 1132 435
Size: 414 248 35 2722
Fragments arranged by size:
2722 414 248 35
BanII G_rGCy'C
Cuts at: 338 433 1463 338
Size: 95 1030 2294
Fragments arranged by size:
2294 1030 95
BbeI G_GCGC'C
Cuts at: 853 1101 853
Size: 248 3171
BbsI GAAGACnn'nnnn_
Cuts at: 397 397
Size: 3419
BbvCI CC'TCA_GC
Cuts at: 242 242
Size: 3419
BciVI GTATCCnnnnn_n'
Cuts at: 1317 2078 3419 1317
Size: 761 1341 1317
Fragments arranged by size:
1341 1317 761
BclI T'GATC_A
Cuts at: 939 939
Size: 3419
BglII A'GATC_T
Cuts at: 934 2971 934
Size: 2037 1382
BlpI GC'TnA_GC
Cuts at: 531 531
Size: 3419
Bme1580I G_kGCm'C
Cuts at: 338 1044 1137 2193 338
Size: 706 93 1056 1564
Fragments arranged by size:
1564 1056 706 93
BmrI ACTGGGnnnn_n'
Cuts at: 631 712 1047 3191 631
Size: 81 335 2144 859
Fragments arranged by size:
2144 859 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3419
Bpu10I CC'TnA_GC
Cuts at: 242 242
Size: 3419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1474 1966 2264 595
Size: 879 492 298 1750
Fragments arranged by size:
1750 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3123 3123
Size: 3419
BsaAI yAC'GTr
Cuts at: 772 772
Size: 3419
BsaBI GATnn'nnATC
Cuts at: 6 933 2970 6
Size: 927 2037 455
Fragments arranged by size:
2037 927 455
BsaHI Gr'CG_yC
Cuts at: 850 1098 1800 3296 850
Size: 248 702 1496 973
Fragments arranged by size:
1496 973 702 248
BsaWI w'CCGG_w
Cuts at: 608 1129 1807 2081 2228 2639 608
Size: 521 678 274 147 411 1388
Fragments arranged by size:
1388 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 238 241 244 259 292 376 196
Size: 42 3 3 15 33 84 3239
Fragments arranged by size:
3239 84 42 33 15 3 3
BseYI C'CCAG_C
Cuts at: 844 1602 2179 2675 844
Size: 758 577 496 1588
Fragments arranged by size:
1588 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 137 961 1007 2215 104
Size: 33 824 46 1208 1308
Fragments arranged by size:
1308 1208 824 46 33
BsiHKAI G_wGCw'C
Cuts at: 433 1211 1401 2193 433
Size: 778 190 792 1659
Fragments arranged by size:
1659 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 338 433 1044 1137 1211 1401 1463 2193 338
Size: 95 611 93 74 190 62 730 1564
Fragments arranged by size:
1564 730 611 190 95 93 74 62
BspEI T'CCGG_A
Cuts at: 2639 2639
Size: 3419
BspHI T'CATG_A
Cuts at: 2595 2595
Size: 3419
BspMI ACCTGCnnnn'nnnn_
Cuts at: 469 683 985 1366 469
Size: 214 302 381 2522
Fragments arranged by size:
2522 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1711 1765 3249 3414 50
Size: 1661 54 1484 165 55
Fragments arranged by size:
1661 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1331 1331
Size: 3419
BsrFI r'CCGG_y
Cuts at: 1417 1598 1807 1417
Size: 181 209 3029
Fragments arranged by size:
3029 209 181
BssSI C'ACGA_G
Cuts at: 1690 2048 1690
Size: 358 3061
BstAPI GCAn_nnn'nTGC
Cuts at: 796 796
Size: 3419
BstBI TT'CG_AA
Cuts at: 1780 1780
Size: 3419
BstYI r'GATC_y
Cuts at: 1 375 444 911 934 1269 1515 2527 2613
Size: 374 69 467 23 335 246 1012 86
Cuts at: 2613 2625 2971 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 374 346 335 246 86
69 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1369 1550 1713 1369
Size: 181 163 3075
Fragments arranged by size:
3075 181 163
BtsI GCAGTG_nn'
Cuts at: 662 662
Size: 3419
DraI TTT'AAA
Cuts at: 418 418
Size: 3419
DrdI GACnn_nn'nnGTC
Cuts at: 1125 1983 1125
Size: 858 2561
EaeI y'GGCC_r
Cuts at: 134 1004 1178 1569 1596 2941 134
Size: 870 174 391 27 1345 612
Fragments arranged by size:
1345 870 612 391 174 27
EagI C'GGCC_G
Cuts at: 134 1004 134
Size: 870 2549
EarI CTCTTCn'nnn_
Cuts at: 314 340 353 1442 1652 314
Size: 26 13 1089 210 2081
Fragments arranged by size:
2081 1089 210 26 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1627 1937 2083 3229 1627
Size: 310 146 1146 1817
Fragments arranged by size:
1817 1146 310 146
EcoICRI GAG'CTC
Cuts at: 431 431
Size: 3419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 364 1244 1676 2423 2691 178
Size: 186 880 432 747 268 906
Fragments arranged by size:
906 880 747 432 268 186
EcoO109I rG'GnC_Cy
Cuts at: 153 334 558 153
Size: 181 224 3014
Fragments arranged by size:
3014 224 181
EcoRI G'AATT_C
Cuts at: 423 423
Size: 3419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1438 1438
Size: 3419
FspI TGC'GCA
Cuts at: 1200 1200
Size: 3419
HaeII r_GCGC'y
Cuts at: 149 853 1101 2123 3102 149
Size: 704 248 1022 979 466
Fragments arranged by size:
1022 979 704 466 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1261 1291 1293 1323 1261
Size: 30 2 30 3357
Fragments arranged by size:
3357 30 30 2
HincII GTy'rAC
Cuts at: 458 458
Size: 3419
KasI G'GCGC_C
Cuts at: 849 1097 849
Size: 248 3171
KpnI G_GTAC'C
Cuts at: 439 439
Size: 3419
MluI A'CGCG_T
Cuts at: 2852 2852
Size: 3419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 2090 2274 92
Size: 1998 184 1237
Fragments arranged by size:
1998 1237 184
MscI TGG'CCA
Cuts at: 1180 1180
Size: 3419
MslI CAynn'nnrTG
Cuts at: 1408 1535 1408
Size: 127 3292
MspA1I CmG'CkG
Cuts at: 133 530 844 1204 2217 2462 133
Size: 397 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 397 360 314 245
NaeI GCC'GGC
Cuts at: 1600 1600
Size: 3419
NarI GG'CG_CC
Cuts at: 850 1098 850
Size: 248 3171
NgoMIV G'CCGG_C
Cuts at: 1598 1598
Size: 3419
NspI r_CATG'y
Cuts at: 472 972 1879 472
Size: 500 907 2012
Fragments arranged by size:
2012 907 500
PciI A'CATG_T
Cuts at: 1875 1875
Size: 3419
PmeI GTTT'AAAC
Cuts at: 418 418
Size: 3419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 3387
PpuMI rG'GwC_Cy
Cuts at: 153 153
Size: 3419
PspOMI G'GGCC_C
Cuts at: 334 334
Size: 3419
PstI C_TGCA'G
Cuts at: 466 1151 466
Size: 685 2734
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3419
PvuII CAG'CTG
Cuts at: 844 1204 844
Size: 360 3059
RsrII CG'GwC_CG
Cuts at: 1614 1614
Size: 3419
SacI G_AGCT'C
Cuts at: 433 433
Size: 3419
SalI G'TCGA_C
Cuts at: 456 456
Size: 3419
SapI GCTCTTCn'nnn_
Cuts at: 1442 1652 1442
Size: 210 3209
SbfI CC_TGCA'GG
Cuts at: 466 466
Size: 3419
ScaI AGT'ACT
Cuts at: 2936 2936
Size: 3419
SfcI C'TryA_G
Cuts at: 33 462 1147 2140 2331 33
Size: 429 685 993 191 1121
Fragments arranged by size:
1121 993 685 429 191
SfoI GGC'GCC
Cuts at: 851 1099 851
Size: 248 3171
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3419
SmaI CCC'GGG
Cuts at: 441 441
Size: 3419
SmlI C'TyrA_G
Cuts at: 574 1489 1981 2243 574
Size: 915 492 262 1750
Fragments arranged by size:
1750 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3419
SphI G_CATG'C
Cuts at: 472 972 472
Size: 500 2919
StyI C'CwwG_G
Cuts at: 149 553 907 149
Size: 404 354 2661
Fragments arranged by size:
2661 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1002 1135 1594 1803 1002
Size: 133 459 209 2618
Fragments arranged by size:
2618 459 209 133
TatI w'GTAC_w
Cuts at: 2934 2934
Size: 3419
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3387
Tth111I GACn'n_nGTC
Cuts at: 1216 1216
Size: 3419
XbaI T'CTAG_A
Cuts at: 450 450
Size: 3419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 914 914
Size: 3419
XmaI C'CCGG_G
Cuts at: 439 439
Size: 3419
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI