Restriction map for
pF1KB8266
(Circular) (Six-base) MAPSORT of: pF1KB8266.seq Check: 6443 from: 1 to: 3683
pF1KB8266 3683 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 721 721
Size: 3683
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 988 2555 988
Size: 1567 2116
ApaI G_GGCC'C
Cuts at: 523 523
Size: 3683
ApaLI G'TGCA_C
Cuts at: 2453 2453
Size: 3683
ApoI r'AATT_y
Cuts at: 592 687 3036 3292 592
Size: 95 2349 256 983
Fragments arranged by size:
2349 983 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 261 703 261
Size: 442 3241
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 623 656 623
Size: 33 3650
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 699 1113 1361 1396 699
Size: 414 248 35 2986
Fragments arranged by size:
2986 414 248 35
BanII G_rGCy'C
Cuts at: 523 697 1727 523
Size: 174 1030 2479
Fragments arranged by size:
2479 1030 174
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BbsI GAAGACnn'nnnn_
Cuts at: 633 633
Size: 3683
BciVI GTATCCnnnnn_n'
Cuts at: 624 1581 2342 3683 624
Size: 957 761 1341 624
Fragments arranged by size:
1341 957 761 624
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BglII A'GATC_T
Cuts at: 1198 3235 1198
Size: 2037 1646
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 523 1308 1401 2457 523
Size: 785 93 1056 1749
Fragments arranged by size:
1749 1056 785 93
BmrI ACTGGGnnnn_n'
Cuts at: 450 895 976 1311 3455 450
Size: 445 81 335 2144 678
Fragments arranged by size:
2144 678 445 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1738 2230 2528 859
Size: 879 492 298 2014
Fragments arranged by size:
2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 195 370 466 3387 195
Size: 175 96 2921 491
Fragments arranged by size:
2921 491 175 96
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 1114 1362 2064 3560 1114
Size: 248 702 1496 1237
Fragments arranged by size:
1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 177 428 872 1393 2071 2345 2492 2903 177
Size: 251 444 521 678 274 147 411 957
Fragments arranged by size:
957 678 521 444 411 274 251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 331 361 331
Size: 30 3653
BseYI C'CCAG_C
Cuts at: 147 156 1108 1866 2443 2939 147
Size: 9 952 758 577 496 891
Fragments arranged by size:
952 891 758 577 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 217
Size: 3683
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 697 1475 1665 2457 697
Size: 778 190 792 1923
Fragments arranged by size:
1923 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 523 697 1308 1401 1475 1665 1727 2457 523
Size: 174 611 93 74 190 62 730 1749
Fragments arranged by size:
1749 730 611 190 174 93 74 62
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 733 947 1249 1630 173
Size: 560 214 302 381 2226
Fragments arranged by size:
2226 560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BsrGI T'GTAC_A
Cuts at: 500 500
Size: 3683
BssSI C'ACGA_G
Cuts at: 1954 2312 1954
Size: 358 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 1060
Size: 3683
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstYI r'GATC_y
Cuts at: 1 708 1175 1198 1533 1779 2791 2877 2889
Size: 707 467 23 335 246 1012 86 12
Cuts at: 2889 3235 1
Size: 346 449
Fragments arranged by size:
1012 707 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 358 358
Size: 3683
BtgI C'CryG_G
Cuts at: 107 323 401 107
Size: 216 78 3389
Fragments arranged by size:
3389 216 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1633 1814 1977 1633
Size: 181 163 3339
Fragments arranged by size:
3339 181 163
BtsI GCAGTG_nn'
Cuts at: 926 926
Size: 3683
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DraIII CAC_nnn'GTG
Cuts at: 128 128
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 542 1268 1442 1833 1860 3205 542
Size: 726 174 391 27 1345 1020
Fragments arranged by size:
1345 1020 726 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1891 2201 2347 3493 1891
Size: 310 146 1146 2081
Fragments arranged by size:
2081 1146 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 357 357
Size: 3683
EcoO109I rG'GnC_Cy
Cuts at: 407 519 520 822 407
Size: 112 1 302 3268
Fragments arranged by size:
3268 302 112 1
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1702 1702
Size: 3683
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 234 1117 1365 2387 3366 234
Size: 883 248 1022 979 551
Fragments arranged by size:
1022 979 883 551 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1525 1555 1557 1587 1525
Size: 30 2 30 3621
Fragments arranged by size:
3621 30 30 2
HincII GTy'rAC
Cuts at: 722 722
Size: 3683
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 2354 2538 281
Size: 2073 184 1426
Fragments arranged by size:
2073 1426 184
MscI TGG'CCA
Cuts at: 544 1444 544
Size: 900 2783
MslI CAynn'nnrTG
Cuts at: 1672 1799 1672
Size: 127 3556
MspA1I CmG'CkG
Cuts at: 403 794 1108 1468 2481 2726 403
Size: 391 314 360 1013 245 1360
Fragments arranged by size:
1360 1013 391 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NcoI C'CATG_G
Cuts at: 107 107
Size: 3683
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NspI r_CATG'y
Cuts at: 736 1236 2143 736
Size: 500 907 2276
Fragments arranged by size:
2276 907 500
PasI CC'CwG_GG
Cuts at: 572 615 572
Size: 43 3640
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PfoI T'CCnGG_A
Cuts at: 272 272
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PpuMI rG'GwC_Cy
Cuts at: 407 407
Size: 3683
PspOMI G'GGCC_C
Cuts at: 519 519
Size: 3683
PspXI vC'TCGA_Gb
Cuts at: 261 261
Size: 3683
PstI C_TGCA'G
Cuts at: 170 398 730 1415 170
Size: 228 332 685 2438
Fragments arranged by size:
2438 685 332 228
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 1108 1468 1108
Size: 360 3323
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SacII CC_GC'GG
Cuts at: 404 404
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SanDI GG'GwC_CC
Cuts at: 407 407
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 170 730 170
Size: 560 3123
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 166 394 420 552 726 1411 2404 2595
Size: 133 228 26 132 174 685 993 191
Cuts at: 2595 33
Size: 1121
Fragments arranged by size:
1121 993 685 228 191 174 133 132
26
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 261 838 1753 2245 2507 261
Size: 577 915 492 262 1437
Fragments arranged by size:
1437 915 577 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StyI C'CwwG_G
Cuts at: 107 817 1171 107
Size: 710 354 2619
Fragments arranged by size:
2619 710 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 490 1266 1399 1858 2067 490
Size: 776 133 459 209 2106
Fragments arranged by size:
2106 776 459 209 133
TatI w'GTAC_w
Cuts at: 500 3198 500
Size: 2698 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 3651
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 714 714
Size: 3683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1178 1178
Size: 3683
XhoI C'TCGA_G
Cuts at: 261 261
Size: 3683
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI