Restriction map for
pF1KB8261
(Circular) (Six-base) MAPSORT of: pF1KB8261.seq Check: 8594 from: 1 to: 3578
pF1KB8261 3578 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 616 616
Size: 3578
Acc65I G'GTAC_C
Cuts at: 220 594 220
Size: 374 3204
AclI AA'CG_TT
Cuts at: 3418 3418
Size: 3578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1403 1835 2582 2850 165
Size: 1238 432 747 268 893
Fragments arranged by size:
1238 893 747 432 268
AflIII A'CryG_T
Cuts at: 930 2034 3011 930
Size: 1104 977 1497
Fragments arranged by size:
1497 1104 977
AgeI A'CCGG_T
Cuts at: 1966 1966
Size: 3578
AlwNI CAG_nnn'CTG
Cuts at: 883 2450 883
Size: 1567 2011
ApaLI G'TGCA_C
Cuts at: 2348 2348
Size: 3578
ApoI r'AATT_y
Cuts at: 582 2931 3187 582
Size: 2349 256 973
Fragments arranged by size:
2349 973 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3578
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
AvaI C'yCGr_G
Cuts at: 130 598 130
Size: 468 3110
BamHI G'GATC_C
Cuts at: 603 603
Size: 3578
BanI G'GyrC_C
Cuts at: 160 220 388 466 594 1008 1256 1291 160
Size: 60 168 78 128 414 248 35 2447
Fragments arranged by size:
2447 414 248 168 128 78 60 35
BanII G_rGCy'C
Cuts at: 592 1622 592
Size: 1030 2548
BbeI G_GCGC'C
Cuts at: 164 392 1012 1260 164
Size: 228 620 248 2482
Fragments arranged by size:
2482 620 248 228
BbsI GAAGACnn'nnnn_
Cuts at: 171 171
Size: 3578
BciVI GTATCCnnnnn_n'
Cuts at: 1476 2237 3578 1476
Size: 761 1341 1476
Fragments arranged by size:
1476 1341 761
BclI T'GATC_A
Cuts at: 1098 1098
Size: 3578
BglI GCCn_nnn'nGGC
Cuts at: 413 413
Size: 3578
BglII A'GATC_T
Cuts at: 1093 3130 1093
Size: 2037 1541
BlpI GC'TnA_GC
Cuts at: 690 690
Size: 3578
Bme1580I G_kGCm'C
Cuts at: 469 1203 1296 2352 469
Size: 734 93 1056 1695
Fragments arranged by size:
1695 1056 734 93
BmrI ACTGGGnnnn_n'
Cuts at: 274 790 871 1206 3350 274
Size: 516 81 335 2144 502
Fragments arranged by size:
2144 516 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 364
Size: 3578
Bpu10I CC'TnA_GC
Cuts at: 397 409 397
Size: 12 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1633 2125 2423 754
Size: 879 492 298 1909
Fragments arranged by size:
1909 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 547 3282 547
Size: 2735 843
BsaAI yAC'GTr
Cuts at: 931 931
Size: 3578
BsaBI GATnn'nnATC
Cuts at: 6 511 1092 3129 6
Size: 505 581 2037 455
Fragments arranged by size:
2037 581 505 455
BsaHI Gr'CG_yC
Cuts at: 161 389 1009 1257 1959 3455 161
Size: 228 620 248 702 1496 284
Fragments arranged by size:
1496 702 620 284 248 228
BsaWI w'CCGG_w
Cuts at: 767 1288 1966 2240 2387 2798 767
Size: 521 678 274 147 411 1547
Fragments arranged by size:
1547 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 384 473 1003 1761 2338 2834 384
Size: 89 530 758 577 496 1128
Fragments arranged by size:
1128 758 577 530 496 89
BsiEI CG_ry'CG
Cuts at: 104 170 253 1120 1166 2374 104
Size: 66 83 867 46 1208 1308
Fragments arranged by size:
1308 1208 867 83 66 46
BsiHKAI G_wGCw'C
Cuts at: 530 592 1370 1560 2352 530
Size: 62 778 190 792 1756
Fragments arranged by size:
1756 792 778 190 62
Bsp1286I G_dGCh'C
Cuts at: 469 530 592 1203 1296 1370 1560 1622 2352
Size: 61 62 611 93 74 190 62 730
Cuts at: 2352 469
Size: 1695
Fragments arranged by size:
1695 730 611 190 93 74 62 62
61
BspEI T'CCGG_A
Cuts at: 2798 2798
Size: 3578
BspHI T'CATG_A
Cuts at: 263 2754 263
Size: 2491 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 842 1144 1525 628
Size: 214 302 381 2681
Fragments arranged by size:
2681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 125 218 413 1870 1924 3408 3573 50
Size: 75 93 195 1457 54 1484 165 55
Fragments arranged by size:
1484 1457 195 165 93 75 55 54
BsrDI GCAATG_nn'
Cuts at: 227 1490 227
Size: 1263 2315
BsrFI r'CCGG_y
Cuts at: 223 391 424 1576 1757 1966 223
Size: 168 33 1152 181 209 1835
Fragments arranged by size:
1835 1152 209 181 168 33
BssSI C'ACGA_G
Cuts at: 1849 2207 1849
Size: 358 3220
BstAPI GCAn_nnn'nTGC
Cuts at: 955 955
Size: 3578
BstBI TT'CG_AA
Cuts at: 1939 1939
Size: 3578
BstYI r'GATC_y
Cuts at: 1 603 1070 1093 1428 1674 2686 2772 2784
Size: 602 467 23 335 246 1012 86 12
Cuts at: 2784 3130 1
Size: 346 449
Fragments arranged by size:
1012 602 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 173 173
Size: 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 285 318 1528 1709 1872 285
Size: 33 1210 181 163 1991
Fragments arranged by size:
1991 1210 181 163 33
BtsI GCAGTG_nn'
Cuts at: 821 821
Size: 3578
DraI TTT'AAA
Cuts at: 577 577
Size: 3578
DrdI GACnn_nn'nnGTC
Cuts at: 1284 2142 1284
Size: 858 2720
EaeI y'GGCC_r
Cuts at: 167 233 250 1163 1337 1728 1755 3100 167
Size: 66 17 913 174 391 27 1345 645
Fragments arranged by size:
1345 913 645 391 174 66 27 17
EagI C'GGCC_G
Cuts at: 167 250 1163 167
Size: 83 913 2582
Fragments arranged by size:
2582 913 83
EarI CTCTTCn'nnn_
Cuts at: 1601 1811 1601
Size: 210 3368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1786 2096 2242 3388 1786
Size: 310 146 1146 1976
Fragments arranged by size:
1976 1146 310 146
EcoICRI GAG'CTC
Cuts at: 590 590
Size: 3578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 364 1403 1835 2582 2850 165
Size: 199 1039 432 747 268 893
Fragments arranged by size:
1039 893 747 432 268 199
EcoO109I rG'GnC_Cy
Cuts at: 717 717
Size: 3578
EcoRI G'AATT_C
Cuts at: 582 582
Size: 3578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1597 1597
Size: 3578
FspI TGC'GCA
Cuts at: 1359 1359
Size: 3578
HaeII r_GCGC'y
Cuts at: 164 188 197 392 1012 1260 2282 3261 164
Size: 24 9 195 620 248 1022 979 481
Fragments arranged by size:
1022 979 620 481 248 195 24 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1420 1450 1452 1482 1420
Size: 30 2 30 3516
Fragments arranged by size:
3516 30 30 2
HincII GTy'rAC
Cuts at: 617 617
Size: 3578
KasI G'GCGC_C
Cuts at: 160 388 1008 1256 160
Size: 228 620 248 2482
Fragments arranged by size:
2482 620 248 228
KpnI G_GTAC'C
Cuts at: 224 598 224
Size: 374 3204
MluI A'CGCG_T
Cuts at: 3011 3011
Size: 3578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2433 2249
Size: 184 3394
MscI TGG'CCA
Cuts at: 1339 1339
Size: 3578
MslI CAynn'nnrTG
Cuts at: 280 1567 1694 280
Size: 1287 127 2164
Fragments arranged by size:
2164 1287 127
MspA1I CmG'CkG
Cuts at: 384 689 1003 1363 2376 2621 384
Size: 305 314 360 1013 245 1341
Fragments arranged by size:
1341 1013 360 314 305 245
NaeI GCC'GGC
Cuts at: 393 426 1759 393
Size: 33 1333 2212
Fragments arranged by size:
2212 1333 33
NarI GG'CG_CC
Cuts at: 161 389 1009 1257 161
Size: 228 620 248 2482
Fragments arranged by size:
2482 620 248 228
NcoI C'CATG_G
Cuts at: 173 173
Size: 3578
NgoMIV G'CCGG_C
Cuts at: 391 424 1757 391
Size: 33 1333 2212
Fragments arranged by size:
2212 1333 33
NspI r_CATG'y
Cuts at: 631 1131 2038 631
Size: 500 907 2171
Fragments arranged by size:
2171 907 500
PasI CC'CwG_GG
Cuts at: 189 189
Size: 3578
PciI A'CATG_T
Cuts at: 2034 2034
Size: 3578
PmeI GTTT'AAAC
Cuts at: 577 577
Size: 3578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 2717
Size: 32 3546
PstI C_TGCA'G
Cuts at: 625 1310 625
Size: 685 2893
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3578
PvuII CAG'CTG
Cuts at: 384 1003 1363 384
Size: 619 360 2599
Fragments arranged by size:
2599 619 360
RsrII CG'GwC_CG
Cuts at: 1773 1773
Size: 3578
SacI G_AGCT'C
Cuts at: 592 592
Size: 3578
SalI G'TCGA_C
Cuts at: 615 615
Size: 3578
SapI GCTCTTCn'nnn_
Cuts at: 1601 1811 1601
Size: 210 3368
SbfI CC_TGCA'GG
Cuts at: 625 625
Size: 3578
ScaI AGT'ACT
Cuts at: 3095 3095
Size: 3578
SfcI C'TryA_G
Cuts at: 33 621 1306 2299 2490 33
Size: 588 685 993 191 1121
Fragments arranged by size:
1121 993 685 588 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 413 413
Size: 3578
SfoI GGC'GCC
Cuts at: 162 390 1010 1258 162
Size: 228 620 248 2482
Fragments arranged by size:
2482 620 248 228
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
SmaI CCC'GGG
Cuts at: 600 600
Size: 3578
SmlI C'TyrA_G
Cuts at: 733 1648 2140 2402 733
Size: 915 492 262 1909
Fragments arranged by size:
1909 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3578
SphI G_CATG'C
Cuts at: 631 1131 631
Size: 500 3078
StyI C'CwwG_G
Cuts at: 173 712 1066 173
Size: 539 354 2685
Fragments arranged by size:
2685 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1161 1294 1753 1962 1161
Size: 133 459 209 2777
Fragments arranged by size:
2777 459 209 133
TatI w'GTAC_w
Cuts at: 3093 3093
Size: 3578
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3546
Tth111I GACn'n_nGTC
Cuts at: 1375 1375
Size: 3578
XbaI T'CTAG_A
Cuts at: 609 609
Size: 3578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 312 441 1073 279
Size: 33 129 632 2784
Fragments arranged by size:
2784 632 129 33
XmaI C'CCGG_G
Cuts at: 598 598
Size: 3578
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI