Restriction map for
pF1KB8260
(Circular) (Six-base) MAPSORT of: pF1KB8260.seq Check: 6253 from: 1 to: 3710
pF1KB8260 3710 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 748 748
Size: 3710
Acc65I G'GTAC_C
Cuts at: 726 726
Size: 3710
AclI AA'CG_TT
Cuts at: 3550 3550
Size: 3710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 500 1535 1967 2714 2982 376
Size: 124 1035 432 747 268 1104
Fragments arranged by size:
1104 1035 747 432 268 124
AflIII A'CryG_T
Cuts at: 1062 2166 3143 1062
Size: 1104 977 1629
Fragments arranged by size:
1629 1104 977
AgeI A'CCGG_T
Cuts at: 2098 2098
Size: 3710
AleI CACnn'nnGTG
Cuts at: 574 574
Size: 3710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 592 643 592
Size: 51 3659
AlwNI CAG_nnn'CTG
Cuts at: 689 1015 2582 689
Size: 326 1567 1817
Fragments arranged by size:
1817 1567 326
ApaI G_GGCC'C
Cuts at: 178 178
Size: 3710
ApaLI G'TGCA_C
Cuts at: 400 2480 400
Size: 2080 1630
ApoI r'AATT_y
Cuts at: 714 3063 3319 714
Size: 2349 256 1105
Fragments arranged by size:
2349 1105 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3710
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
AvaI C'yCGr_G
Cuts at: 730 730
Size: 3710
BamHI G'GATC_C
Cuts at: 256 735 256
Size: 479 3231
BanI G'GyrC_C
Cuts at: 429 468 568 611 655 726 1140 1388 1423
Size: 39 100 43 44 71 414 248 35
Cuts at: 1423 429
Size: 2716
Fragments arranged by size:
2716 414 248 100 71 44 43 39
35
BanII G_rGCy'C
Cuts at: 178 724 1754 178
Size: 546 1030 2134
Fragments arranged by size:
2134 1030 546
BbeI G_GCGC'C
Cuts at: 659 1144 1392 659
Size: 485 248 2977
Fragments arranged by size:
2977 485 248
BbvCI CC'TCA_GC
Cuts at: 460 460
Size: 3710
BciVI GTATCCnnnnn_n'
Cuts at: 235 1608 2369 3710 235
Size: 1373 761 1341 235
Fragments arranged by size:
1373 1341 761 235
BclI T'GATC_A
Cuts at: 1230 1230
Size: 3710
BglI GCCn_nnn'nGGC
Cuts at: 140 140
Size: 3710
BglII A'GATC_T
Cuts at: 1225 3262 1225
Size: 2037 1673
BlpI GC'TnA_GC
Cuts at: 409 822 409
Size: 413 3297
Bme1580I G_kGCm'C
Cuts at: 178 404 473 1335 1428 2484 178
Size: 226 69 862 93 1056 1404
Fragments arranged by size:
1404 1056 862 226 93 69
BmgBI CAC'GTC
Cuts at: 270 270
Size: 3710
BmrI ACTGGGnnnn_n'
Cuts at: 391 922 1003 1338 3482 391
Size: 531 81 335 2144 619
Fragments arranged by size:
2144 619 531 335 81
BmtI G_CTAG'C
Cuts at: 550 550
Size: 3710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 3710
Bpu10I CC'TnA_GC
Cuts at: 460 460
Size: 3710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 886 1765 2257 2555 436
Size: 450 879 492 298 1591
Fragments arranged by size:
1591 879 492 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 3414 3414
Size: 3710
BsaAI yAC'GTr
Cuts at: 1063 1063
Size: 3710
BsaBI GATnn'nnATC
Cuts at: 6 1224 3261 6
Size: 1218 2037 455
Fragments arranged by size:
2037 1218 455
BsaHI Gr'CG_yC
Cuts at: 524 656 1141 1389 2091 3587 524
Size: 132 485 248 702 1496 647
Fragments arranged by size:
1496 702 647 485 248 132
BsaWI w'CCGG_w
Cuts at: 168 218 899 1420 2098 2372 2519 2930 168
Size: 50 681 521 678 274 147 411 948
Fragments arranged by size:
948 681 678 521 411 274 147 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 550 448
Size: 102 3608
BseYI C'CCAG_C
Cuts at: 439 1135 1893 2470 2966 439
Size: 696 758 577 496 1183
Fragments arranged by size:
1183 758 696 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 430 610 349
Size: 81 180 3449
Fragments arranged by size:
3449 180 81
BsiEI CG_ry'CG
Cuts at: 104 1252 1298 2506 104
Size: 1148 46 1208 1308
Fragments arranged by size:
1308 1208 1148 46
BsiHKAI G_wGCw'C
Cuts at: 404 683 724 1502 1692 2484 404
Size: 279 41 778 190 792 1630
Fragments arranged by size:
1630 792 778 279 190 41
Bsp1286I G_dGCh'C
Cuts at: 178 404 473 683 724 1335 1428 1502 1692
Size: 226 69 210 41 611 93 74 190
Cuts at: 1692 1754 2484 178
Size: 62 730 1404
Fragments arranged by size:
1404 730 611 226 210 190 93 74
69 62 41
BspEI T'CCGG_A
Cuts at: 218 2930 218
Size: 2712 998
BspHI T'CATG_A
Cuts at: 2886 2886
Size: 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 974 1276 1657 760
Size: 214 302 381 2813
Fragments arranged by size:
2813 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2002 2056 3540 3705 50
Size: 1952 54 1484 165 55
Fragments arranged by size:
1952 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 191 1622 191
Size: 1431 2279
BsrFI r'CCGG_y
Cuts at: 608 1708 1889 2098 608
Size: 1100 181 209 2220
Fragments arranged by size:
2220 1100 209 181
BssSI C'ACGA_G
Cuts at: 1981 2339 1981
Size: 358 3352
BstAPI GCAn_nnn'nTGC
Cuts at: 1087 1087
Size: 3710
BstBI TT'CG_AA
Cuts at: 2071 2071
Size: 3710
BstEII G'GTnAC_C
Cuts at: 309 309
Size: 3710
BstXI CCAn_nnnn'nTGG
Cuts at: 633 633
Size: 3710
BstYI r'GATC_y
Cuts at: 1 256 359 735 1202 1225 1560 1806 2818
Size: 255 103 376 467 23 335 246 1012
Cuts at: 2818 2904 2916 3262 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 376 346 335 255 246
103 86 23 12
BtgI C'CryG_G
Cuts at: 107 615 107
Size: 508 3202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1660 1841 2004 1660
Size: 181 163 3366
Fragments arranged by size:
3366 181 163
BtsI GCAGTG_nn'
Cuts at: 953 953
Size: 3710
DraI TTT'AAA
Cuts at: 709 709
Size: 3710
DrdI GACnn_nn'nnGTC
Cuts at: 301 1416 2274 301
Size: 1115 858 1737
Fragments arranged by size:
1737 1115 858
EaeI y'GGCC_r
Cuts at: 1295 1469 1860 1887 3232 1295
Size: 174 391 27 1345 1773
Fragments arranged by size:
1773 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1295 1295
Size: 3710
EarI CTCTTCn'nnn_
Cuts at: 170 1733 1943 170
Size: 1563 210 1937
Fragments arranged by size:
1937 1563 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1918 2228 2374 3520 1918
Size: 310 146 1146 2108
Fragments arranged by size:
2108 1146 310 146
EcoICRI GAG'CTC
Cuts at: 722 722
Size: 3710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 500 1535 1967 2714 2982 376
Size: 124 1035 432 747 268 1104
Fragments arranged by size:
1104 1035 747 432 268 124
EcoNI CCTnn'n_nnAGG
Cuts at: 532 532
Size: 3710
EcoO109I rG'GnC_Cy
Cuts at: 175 849 175
Size: 674 3036
EcoRI G'AATT_C
Cuts at: 714 714
Size: 3710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 366 1729 366
Size: 1363 2347
FspI TGC'GCA
Cuts at: 1491 1491
Size: 3710
HaeII r_GCGC'y
Cuts at: 659 1144 1392 2414 3393 659
Size: 485 248 1022 979 976
Fragments arranged by size:
1022 979 976 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1552 1582 1584 1614 1552
Size: 30 2 30 3648
Fragments arranged by size:
3648 30 30 2
HincII GTy'rAC
Cuts at: 749 749
Size: 3710
KasI G'GCGC_C
Cuts at: 655 1140 1388 655
Size: 485 248 2977
Fragments arranged by size:
2977 485 248
KpnI G_GTAC'C
Cuts at: 730 730
Size: 3710
MluI A'CGCG_T
Cuts at: 3143 3143
Size: 3710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2565 2381
Size: 184 3526
MscI TGG'CCA
Cuts at: 1471 1471
Size: 3710
MslI CAynn'nnrTG
Cuts at: 278 371 574 625 1699 1826 278
Size: 93 203 51 1074 127 2162
Fragments arranged by size:
2162 1074 203 127 93 51
MspA1I CmG'CkG
Cuts at: 821 1135 1495 2508 2753 821
Size: 314 360 1013 245 1778
Fragments arranged by size:
1778 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1891 1891
Size: 3710
NarI GG'CG_CC
Cuts at: 656 1141 1389 656
Size: 485 248 2977
Fragments arranged by size:
2977 485 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3710
NgoMIV G'CCGG_C
Cuts at: 1889 1889
Size: 3710
NheI G'CTAG_C
Cuts at: 546 546
Size: 3710
NspI r_CATG'y
Cuts at: 763 1263 2170 763
Size: 500 907 2303
Fragments arranged by size:
2303 907 500
PciI A'CATG_T
Cuts at: 2166 2166
Size: 3710
PmeI GTTT'AAAC
Cuts at: 709 709
Size: 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 3678
PspOMI G'GGCC_C
Cuts at: 174 174
Size: 3710
PstI C_TGCA'G
Cuts at: 117 201 757 1442 117
Size: 84 556 685 2385
Fragments arranged by size:
2385 685 556 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3710
PvuII CAG'CTG
Cuts at: 1135 1495 1135
Size: 360 3350
RsrII CG'GwC_CG
Cuts at: 1905 1905
Size: 3710
SacI G_AGCT'C
Cuts at: 724 724
Size: 3710
SalI G'TCGA_C
Cuts at: 747 747
Size: 3710
SapI GCTCTTCn'nnn_
Cuts at: 170 1733 1943 170
Size: 1563 210 1937
Fragments arranged by size:
1937 1563 210
SbfI CC_TGCA'GG
Cuts at: 757 757
Size: 3710
ScaI AGT'ACT
Cuts at: 505 3227 505
Size: 2722 988
SfcI C'TryA_G
Cuts at: 33 113 197 753 1438 2431 2622 33
Size: 80 84 556 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 191 84 80
SfoI GGC'GCC
Cuts at: 657 1142 1390 657
Size: 485 248 2977
Fragments arranged by size:
2977 485 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
SmaI CCC'GGG
Cuts at: 732 732
Size: 3710
SmlI C'TyrA_G
Cuts at: 451 865 1780 2272 2534 451
Size: 414 915 492 262 1627
Fragments arranged by size:
1627 915 492 414 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3710
SphI G_CATG'C
Cuts at: 763 1263 763
Size: 500 3210
StyI C'CwwG_G
Cuts at: 107 844 1198 107
Size: 737 354 2619
Fragments arranged by size:
2619 737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 328 1293 1426 1885 2094 328
Size: 965 133 459 209 1944
Fragments arranged by size:
1944 965 459 209 133
TatI w'GTAC_w
Cuts at: 503 3225 503
Size: 2722 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 3678
Tth111I GACn'n_nGTC
Cuts at: 1507 1507
Size: 3710
XbaI T'CTAG_A
Cuts at: 741 741
Size: 3710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1205 1205
Size: 3710
XmaI C'CCGG_G
Cuts at: 730 730
Size: 3710
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BpmI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI