Restriction map for
pF1KB8256
(Circular) (Six-base) MAPSORT of: pF1KB8256.seq Check: 1958 from: 1 to: 4178
pF1KB8256 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 617 617
Size: 4178
AccI GT'mk_AC
Cuts at: 155 940 1216 155
Size: 785 276 3117
Fragments arranged by size:
3117 785 276
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 2003 2435 3182 3450 730
Size: 1273 432 747 268 1458
Fragments arranged by size:
1458 1273 747 432 268
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 1103 2566 1103
Size: 1463 2715
AhdI GACnn_n'nnGTC
Cuts at: 270 270
Size: 4178
AleI CACnn'nnGTG
Cuts at: 631 631
Size: 4178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 334 334
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 183 873 1483 3050 183
Size: 690 610 1567 1311
Fragments arranged by size:
1567 1311 690 610
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 385 1182 3531 3787 385
Size: 797 2349 256 776
Fragments arranged by size:
2349 797 776 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 743 1198 743
Size: 455 3723
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 605 747 1194 1608 1856 1891 605
Size: 142 447 414 248 35 2892
Fragments arranged by size:
2892 447 414 248 142 35
BanII G_rGCy'C
Cuts at: 961 1041 1192 2222 961
Size: 80 151 1030 2917
Fragments arranged by size:
2917 1030 151 80
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 1134 1155 1134
Size: 21 4157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 201 235 201
Size: 34 4144
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglI GCCn_nnn'nGGC
Cuts at: 599 599
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 560 1290 560
Size: 730 3448
Bme1580I G_kGCm'C
Cuts at: 750 1803 1896 2952 750
Size: 1053 93 1056 1976
Fragments arranged by size:
1976 1056 1053 93
BmrI ACTGGGnnnn_n'
Cuts at: 232 1390 1471 1806 3950 232
Size: 1158 81 335 2144 460
Fragments arranged by size:
2144 1158 460 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 756 975 2545 756
Size: 219 1570 2389
Fragments arranged by size:
2389 1570 219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 550 463
Size: 87 4091
Bpu10I CC'TnA_GC
Cuts at: 421 421
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1354 2233 2725 3023 1060
Size: 294 879 492 298 2215
Fragments arranged by size:
2215 879 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 169 642 3882 169
Size: 473 3240 465
Fragments arranged by size:
3240 473 465
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1103 1367 1888 2566 2840 2987 3398 1103
Size: 264 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 264 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 286
Size: 30 4148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 735 735
Size: 4178
BseYI C'CCAG_C
Cuts at: 1040 1603 2361 2938 3434 1040
Size: 563 758 577 496 1784
Fragments arranged by size:
1784 758 577 563 496
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1192 1970 2160 2952 1192
Size: 778 190 792 2418
Fragments arranged by size:
2418 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 750 961 1041 1192 1803 1896 1970 2160 2222
Size: 211 80 151 611 93 74 190 62
Cuts at: 2222 2952 750
Size: 730 1976
Fragments arranged by size:
1976 730 611 211 190 151 93 80
74 62
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 617 1228 1442 1744 2125 617
Size: 611 214 302 381 2670
Fragments arranged by size:
2670 611 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 602 1103 2176 2357 2566 602
Size: 501 1073 181 209 2214
Fragments arranged by size:
2214 1073 501 209 181
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 1070 1203 1670 1693 2028 2274 3286 3372
Size: 1069 133 467 23 335 246 1012 86
Cuts at: 3372 3384 3730 1
Size: 12 346 449
Fragments arranged by size:
1069 1012 467 449 346 335 246 133
86 23 12
BtgI C'CryG_G
Cuts at: 115 115
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 204 2128 2309 2472 204
Size: 1924 181 163 1910
Fragments arranged by size:
1924 1910 181 163
BtsI GCAGTG_nn'
Cuts at: 387 500 1421 387
Size: 113 921 3144
Fragments arranged by size:
3144 921 113
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 398 600 1763 1937 2328 2355 3700 398
Size: 202 1163 174 391 27 1345 876
Fragments arranged by size:
1345 1163 876 391 202 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 500 2201 2411 500
Size: 1701 210 2267
Fragments arranged by size:
2267 1701 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 550 730 2003 2435 3182 3450 463
Size: 87 180 1273 432 747 268 1191
Fragments arranged by size:
1273 1191 747 432 268 180 87
EcoO109I rG'GnC_Cy
Cuts at: 180 1317 180
Size: 1137 3041
EcoRI G'AATT_C
Cuts at: 385 1182 385
Size: 797 3381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 495 2197 495
Size: 1702 2476
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 286 288 318 664 696 840 872 2020
Size: 30 2 30 346 32 144 32 1148
Cuts at: 2020 2050 2052 2082 256
Size: 30 2 30 2352
Fragments arranged by size:
2352 1148 346 144 32 32 30 30
30 30 2 2
HincII GTy'rAC
Cuts at: 941 1217 941
Size: 276 3902
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MfeI C'AATT_G
Cuts at: 944 944
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 396 647 1124 2849 3033 396
Size: 251 477 1725 184 1541
Fragments arranged by size:
1725 1541 477 251 184
MscI TGG'CCA
Cuts at: 400 1939 400
Size: 1539 2639
MslI CAynn'nnrTG
Cuts at: 394 631 844 1156 2167 2294 394
Size: 237 213 312 1011 127 2278
Fragments arranged by size:
2278 1011 312 237 213 127
MspA1I CmG'CkG
Cuts at: 117 873 1044 1289 1603 1963 2976 3221 117
Size: 756 171 245 314 360 1013 245 1074
Fragments arranged by size:
1074 1013 756 360 314 245 245 171
NaeI GCC'GGC
Cuts at: 604 2359 604
Size: 1755 2423
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NgoMIV G'CCGG_C
Cuts at: 602 2357 602
Size: 1755 2423
NspI r_CATG'y
Cuts at: 1231 1731 2638 1231
Size: 500 907 2771
Fragments arranged by size:
2771 907 500
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PflMI CCAn_nnn'nTGG
Cuts at: 1106 1106
Size: 4178
PfoI T'CCnGG_A
Cuts at: 566 566
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 180 180
Size: 4178
PshAI GACnn'nnGTC
Cuts at: 178 247 178
Size: 69 4109
PstI C_TGCA'G
Cuts at: 323 614 878 1225 1910 323
Size: 291 264 347 685 2591
Fragments arranged by size:
2591 685 347 291 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 873 1044 1603 1963 873
Size: 171 559 360 3088
Fragments arranged by size:
3088 559 360 171
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SacII CC_GC'GG
Cuts at: 118 118
Size: 4178
SalI G'TCGA_C
Cuts at: 939 1215 939
Size: 276 3902
SapI GCTCTTCn'nnn_
Cuts at: 500 2201 2411 500
Size: 1701 210 2267
Fragments arranged by size:
2267 1701 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 1081 3695 1081
Size: 2614 1564
SfcI C'TryA_G
Cuts at: 33 319 610 874 1221 1906 2899 3090 33
Size: 286 291 264 347 685 993 191 1121
Fragments arranged by size:
1121 993 685 347 291 286 264 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 745 1200 745
Size: 455 3723
SmlI C'TyrA_G
Cuts at: 1075 1333 2248 2740 3002 1075
Size: 258 915 492 262 2251
Fragments arranged by size:
2251 915 492 262 258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 584 827 1312 1666 584
Size: 243 485 354 3096
Fragments arranged by size:
3096 485 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1761 1894 2353 2562 1761
Size: 133 459 209 3377
Fragments arranged by size:
3377 459 209 133
TatI w'GTAC_w
Cuts at: 1079 3693 1079
Size: 2614 1564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 787 846 1673 630
Size: 157 59 827 3135
Fragments arranged by size:
3135 827 157 59
XmaI C'CCGG_G
Cuts at: 743 1198 743
Size: 455 3723
XmnI GAAnn'nnTTC
Cuts at: 1007 1007
Size: 4178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI