Restriction map for
pF1KB8255
(Circular) (Six-base) MAPSORT of: pF1KB8255.seq Check: 1670 from: 1 to: 4064
pF1KB8255 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1029 1029
Size: 4064
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 813 937 1889 2321 3068 3336 679
Size: 134 124 952 432 747 268 1407
Fragments arranged by size:
1407 952 747 432 268 134 124
AflIII A'CryG_T
Cuts at: 1056 1416 2520 3497 1056
Size: 360 1104 977 1623
Fragments arranged by size:
1623 1104 977 360
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AleI CACnn'nnGTG
Cuts at: 960 960
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 999 1369 2936 999
Size: 370 1567 2127
Fragments arranged by size:
2127 1567 370
ApaLI G'TGCA_C
Cuts at: 963 2834 963
Size: 1871 2193
ApoI r'AATT_y
Cuts at: 266 1068 3417 3673 266
Size: 802 2349 256 657
Fragments arranged by size:
2349 802 657 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 212 479 1084 212
Size: 267 605 3192
Fragments arranged by size:
3192 605 267
BamHI G'GATC_C
Cuts at: 718 1089 718
Size: 371 3693
BanI G'GyrC_C
Cuts at: 133 391 1080 1494 1742 1777 133
Size: 258 689 414 248 35 2420
Fragments arranged by size:
2420 689 414 258 248 35
BanII G_rGCy'C
Cuts at: 1078 2108 1078
Size: 1030 3034
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbsI GAAGACnn'nnnn_
Cuts at: 667 667
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglI GCCn_nnn'nGGC
Cuts at: 152 683 152
Size: 531 3533
BglII A'GATC_T
Cuts at: 878 1579 3616 878
Size: 701 2037 1326
Fragments arranged by size:
2037 1326 701
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 394 967 1689 1782 2838 394
Size: 573 722 93 1056 1620
Fragments arranged by size:
1620 1056 722 573 93
BmrI ACTGGGnnnn_n'
Cuts at: 954 1276 1357 1692 3836 954
Size: 322 81 335 2144 1182
Fragments arranged by size:
2144 1182 335 322 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 564 2431 564
Size: 1867 2197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 755 695
Size: 60 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1240 2119 2611 2909 124
Size: 1116 879 492 298 1279
Fragments arranged by size:
1279 1116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 3768 247
Size: 3521 543
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 693 723 693
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 312 438 312
Size: 126 3938
BseYI C'CCAG_C
Cuts at: 155 614 868 1489 2247 2824 3320 155
Size: 459 254 621 758 577 496 899
Fragments arranged by size:
899 758 621 577 496 459 254
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 282 967 1078 1856 2046 2838 282
Size: 685 111 778 190 792 1508
Fragments arranged by size:
1508 792 778 685 190 111
BsmBI CGTCTCn'nnnn_
Cuts at: 609 609
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 282 394 967 1078 1689 1782 1856 2046 2108
Size: 112 573 111 611 93 74 190 62
Cuts at: 2108 2838 282
Size: 730 1508
Fragments arranged by size:
1508 730 611 573 190 112 111 93
74 62
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1029 1114 1328 1630 2011 1029
Size: 85 214 302 381 3082
Fragments arranged by size:
3082 381 302 214 85
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 458 2062 2243 2452 458
Size: 1604 181 209 2070
Fragments arranged by size:
2070 1604 209 181
BssSI C'ACGA_G
Cuts at: 399 2335 2693 399
Size: 1936 358 1770
Fragments arranged by size:
1936 1770 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstEII G'GTnAC_C
Cuts at: 159 1041 159
Size: 882 3182
BstYI r'GATC_y
Cuts at: 1 378 475 718 765 878 1089 1556 1579
Size: 377 97 243 47 113 211 467 23
Cuts at: 1579 1914 2160 3172 3258 3270 3616 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 377 346 335 246 243
211 113 97 86 47 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1307
Size: 4064
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DraIII CAC_nnn'GTG
Cuts at: 277 277
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 625 1770 2628 625
Size: 1145 858 2061
Fragments arranged by size:
2061 1145 858
EaeI y'GGCC_r
Cuts at: 330 675 747 990 1649 1823 2214 2241 3586
Size: 345 72 243 659 174 391 27 1345
Cuts at: 3586 330
Size: 808
Fragments arranged by size:
1345 808 659 391 345 243 174 72
27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 195 2272 2582 2728 3874 195
Size: 2077 310 146 1146 385
Fragments arranged by size:
2077 1146 385 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 695 755 813 937 1889 2321 3068 3336
Size: 16 60 58 124 952 432 747 268
Cuts at: 3336 679
Size: 1407
Fragments arranged by size:
1407 952 747 432 268 124 60 58
16
EcoO109I rG'GnC_Cy
Cuts at: 865 1203 865
Size: 338 3726
EcoRI G'AATT_C
Cuts at: 266 1068 266
Size: 802 3262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 693 725 1906 1936 1938 1968 544
Size: 32 117 32 1181 30 2 30 2640
Fragments arranged by size:
2640 1181 117 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 1056 3497 1056
Size: 2441 1623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 332 677 749 992 1825 332
Size: 345 72 243 833 2571
Fragments arranged by size:
2571 833 345 243 72
MslI CAynn'nnrTG
Cuts at: 229 960 2053 2180 229
Size: 731 1093 127 2113
Fragments arranged by size:
2113 1093 731 127
MspA1I CmG'CkG
Cuts at: 1175 1489 1849 2862 3107 1175
Size: 314 360 1013 245 2132
Fragments arranged by size:
2132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 1117 1617 2524 1117
Size: 500 907 2657
Fragments arranged by size:
2657 907 500
PasI CC'CwG_GG
Cuts at: 510 510
Size: 4064
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 761 761
Size: 4064
PfoI T'CCnGG_A
Cuts at: 188 533 188
Size: 345 3719
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PstI C_TGCA'G
Cuts at: 1111 1796 1111
Size: 685 3379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SexAI A'CCwGG_T
Cuts at: 1022 1022
Size: 4064
SfcI C'TryA_G
Cuts at: 33 1107 1792 2785 2976 33
Size: 1074 685 993 191 1121
Fragments arranged by size:
1121 1074 993 685 191
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 139 212 479 1219 2134 2626 2888 139
Size: 73 267 740 915 492 262 1315
Fragments arranged by size:
1315 915 740 492 267 262 73
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StyI C'CwwG_G
Cuts at: 422 1198 1552 422
Size: 776 354 2934
Fragments arranged by size:
2934 776 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 502 546 1647 1780 2239 2448 502
Size: 44 1101 133 459 209 2118
Fragments arranged by size:
2118 1101 459 209 133 44
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XhoI C'TCGA_G
Cuts at: 212 479 212
Size: 267 3797
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI