Restriction map for
pF1KB8250
(Circular) (Six-base) MAPSORT of: pF1KB8250.seq Check: 8699 from: 1 to: 3479
pF1KB8250 3479 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 517 517
Size: 3479
Acc65I G'GTAC_C
Cuts at: 495 495
Size: 3479
AclI AA'CG_TT
Cuts at: 3319 3319
Size: 3479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 301 1304 1736 2483 2751 154
Size: 147 1003 432 747 268 882
Fragments arranged by size:
1003 882 747 432 268 147
AflIII A'CryG_T
Cuts at: 284 831 1935 2912 284
Size: 547 1104 977 851
Fragments arranged by size:
1104 977 851 547
AgeI A'CCGG_T
Cuts at: 1867 1867
Size: 3479
AlwNI CAG_nnn'CTG
Cuts at: 784 2351 784
Size: 1567 1912
ApaI G_GGCC'C
Cuts at: 471 471
Size: 3479
ApaLI G'TGCA_C
Cuts at: 2249 2249
Size: 3479
ApoI r'AATT_y
Cuts at: 483 2832 3088 483
Size: 2349 256 874
Fragments arranged by size:
2349 874 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3479
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3479
AvaI C'yCGr_G
Cuts at: 499 499
Size: 3479
BamHI G'GATC_C
Cuts at: 504 504
Size: 3479
BanI G'GyrC_C
Cuts at: 495 909 1157 1192 495
Size: 414 248 35 2782
Fragments arranged by size:
2782 414 248 35
BanII G_rGCy'C
Cuts at: 471 493 1523 471
Size: 22 1030 2427
Fragments arranged by size:
2427 1030 22
BbeI G_GCGC'C
Cuts at: 913 1161 913
Size: 248 3231
BbvCI CC'TCA_GC
Cuts at: 431 431
Size: 3479
BciVI GTATCCnnnnn_n'
Cuts at: 1377 2138 3479 1377
Size: 761 1341 1377
Fragments arranged by size:
1377 1341 761
BclI T'GATC_A
Cuts at: 999 999
Size: 3479
BglII A'GATC_T
Cuts at: 994 3031 994
Size: 2037 1442
BlpI GC'TnA_GC
Cuts at: 591 591
Size: 3479
Bme1580I G_kGCm'C
Cuts at: 471 1104 1197 2253 471
Size: 633 93 1056 1697
Fragments arranged by size:
1697 1056 633 93
BmrI ACTGGGnnnn_n'
Cuts at: 691 772 1107 3251 691
Size: 81 335 2144 919
Fragments arranged by size:
2144 919 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 430
Size: 3479
Bpu10I CC'TnA_GC
Cuts at: 431 431
Size: 3479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1534 2026 2324 655
Size: 879 492 298 1810
Fragments arranged by size:
1810 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3183 3183
Size: 3479
BsaAI yAC'GTr
Cuts at: 832 832
Size: 3479
BsaBI GATnn'nnATC
Cuts at: 6 993 3030 6
Size: 987 2037 455
Fragments arranged by size:
2037 987 455
BsaHI Gr'CG_yC
Cuts at: 910 1158 1860 3356 910
Size: 248 702 1496 1033
Fragments arranged by size:
1496 1033 702 248
BsaWI w'CCGG_w
Cuts at: 668 1189 1867 2141 2288 2699 668
Size: 521 678 274 147 411 1448
Fragments arranged by size:
1448 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 904 1662 2239 2735 904
Size: 758 577 496 1648
Fragments arranged by size:
1648 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1021 1067 2275 104
Size: 917 46 1208 1308
Fragments arranged by size:
1308 1208 917 46
BsiHKAI G_wGCw'C
Cuts at: 493 1271 1461 2253 493
Size: 778 190 792 1719
Fragments arranged by size:
1719 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 471 493 1104 1197 1271 1461 1523 2253 471
Size: 22 611 93 74 190 62 730 1697
Fragments arranged by size:
1697 730 611 190 93 74 62 22
BspEI T'CCGG_A
Cuts at: 2699 2699
Size: 3479
BspHI T'CATG_A
Cuts at: 260 2655 260
Size: 2395 1084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 529 743 1045 1426 529
Size: 214 302 381 2582
Fragments arranged by size:
2582 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1771 1825 3309 3474 50
Size: 1721 54 1484 165 55
Fragments arranged by size:
1721 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1391 1391
Size: 3479
BsrFI r'CCGG_y
Cuts at: 1477 1658 1867 1477
Size: 181 209 3089
Fragments arranged by size:
3089 209 181
BssSI C'ACGA_G
Cuts at: 1750 2108 1750
Size: 358 3121
BstAPI GCAn_nnn'nTGC
Cuts at: 856 856
Size: 3479
BstBI TT'CG_AA
Cuts at: 1840 1840
Size: 3479
BstYI r'GATC_y
Cuts at: 1 123 504 971 994 1329 1575 2587 2673
Size: 122 381 467 23 335 246 1012 86
Cuts at: 2673 2685 3031 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 381 346 335 246 122
86 23 12
BtgI C'CryG_G
Cuts at: 107 119 107
Size: 12 3467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1429 1610 1773 1429
Size: 181 163 3135
Fragments arranged by size:
3135 181 163
BtsI GCAGTG_nn'
Cuts at: 722 722
Size: 3479
DraI TTT'AAA
Cuts at: 478 478
Size: 3479
DrdI GACnn_nn'nnGTC
Cuts at: 1185 2043 1185
Size: 858 2621
EaeI y'GGCC_r
Cuts at: 110 1064 1238 1629 1656 3001 110
Size: 954 174 391 27 1345 588
Fragments arranged by size:
1345 954 588 391 174 27
EagI C'GGCC_G
Cuts at: 1064 1064
Size: 3479
EarI CTCTTCn'nnn_
Cuts at: 1502 1712 1502
Size: 210 3269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1687 1997 2143 3289 1687
Size: 310 146 1146 1877
Fragments arranged by size:
1877 1146 310 146
EcoICRI GAG'CTC
Cuts at: 491 491
Size: 3479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 301 430 1304 1736 2483 2751 154
Size: 147 129 874 432 747 268 882
Fragments arranged by size:
882 874 747 432 268 147 129
EcoO109I rG'GnC_Cy
Cuts at: 467 618 467
Size: 151 3328
EcoRI G'AATT_C
Cuts at: 483 483
Size: 3479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 1498 159
Size: 1339 2140
FspI TGC'GCA
Cuts at: 1260 1260
Size: 3479
HaeII r_GCGC'y
Cuts at: 913 1161 2183 3162 913
Size: 248 1022 979 1230
Fragments arranged by size:
1230 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1321 1351 1353 1383 1321
Size: 30 2 30 3417
Fragments arranged by size:
3417 30 30 2
HincII GTy'rAC
Cuts at: 518 518
Size: 3479
KasI G'GCGC_C
Cuts at: 909 1157 909
Size: 248 3231
KpnI G_GTAC'C
Cuts at: 499 499
Size: 3479
MluI A'CGCG_T
Cuts at: 2912 2912
Size: 3479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2150 2334 2150
Size: 184 3295
MscI TGG'CCA
Cuts at: 1240 1240
Size: 3479
MslI CAynn'nnrTG
Cuts at: 1468 1595 1468
Size: 127 3352
MspA1I CmG'CkG
Cuts at: 345 590 904 1264 2277 2522 345
Size: 245 314 360 1013 245 1302
Fragments arranged by size:
1302 1013 360 314 245 245
NaeI GCC'GGC
Cuts at: 1660 1660
Size: 3479
NarI GG'CG_CC
Cuts at: 910 1158 910
Size: 248 3231
NcoI C'CATG_G
Cuts at: 107 107
Size: 3479
NgoMIV G'CCGG_C
Cuts at: 1658 1658
Size: 3479
NspI r_CATG'y
Cuts at: 238 288 532 1032 1939 238
Size: 50 244 500 907 1778
Fragments arranged by size:
1778 907 500 244 50
PciI A'CATG_T
Cuts at: 284 1935 284
Size: 1651 1828
PfoI T'CCnGG_A
Cuts at: 443 443
Size: 3479
PmeI GTTT'AAAC
Cuts at: 478 478
Size: 3479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2650 2618
Size: 32 3447
PspOMI G'GGCC_C
Cuts at: 467 467
Size: 3479
PstI C_TGCA'G
Cuts at: 526 1211 526
Size: 685 2794
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3479
PvuII CAG'CTG
Cuts at: 345 904 1264 345
Size: 559 360 2560
Fragments arranged by size:
2560 559 360
RsrII CG'GwC_CG
Cuts at: 1674 1674
Size: 3479
SacI G_AGCT'C
Cuts at: 493 493
Size: 3479
SalI G'TCGA_C
Cuts at: 516 516
Size: 3479
SapI GCTCTTCn'nnn_
Cuts at: 1502 1712 1502
Size: 210 3269
SbfI CC_TGCA'GG
Cuts at: 526 526
Size: 3479
ScaI AGT'ACT
Cuts at: 2996 2996
Size: 3479
SfcI C'TryA_G
Cuts at: 33 522 1207 2200 2391 33
Size: 489 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 191
SfoI GGC'GCC
Cuts at: 911 1159 911
Size: 248 3231
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3479
SmaI CCC'GGG
Cuts at: 501 501
Size: 3479
SmlI C'TyrA_G
Cuts at: 634 1549 2041 2303 634
Size: 915 492 262 1810
Fragments arranged by size:
1810 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3479
SphI G_CATG'C
Cuts at: 238 532 1032 238
Size: 294 500 2685
Fragments arranged by size:
2685 500 294
StuI AGG'CCT
Cuts at: 142 142
Size: 3479
StyI C'CwwG_G
Cuts at: 107 323 613 967 107
Size: 216 290 354 2619
Fragments arranged by size:
2619 354 290 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1062 1195 1654 1863 1062
Size: 133 459 209 2678
Fragments arranged by size:
2678 459 209 133
TatI w'GTAC_w
Cuts at: 2994 2994
Size: 3479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3447
Tth111I GACn'n_nGTC
Cuts at: 1276 1276
Size: 3479
XbaI T'CTAG_A
Cuts at: 126 510 126
Size: 384 3095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 974 974
Size: 3479
XmaI C'CCGG_G
Cuts at: 499 499
Size: 3479
XmnI GAAnn'nnTTC
Cuts at: 142 332 142
Size: 190 3289
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI