Restriction map for
pF1KB8248
(Circular) (Six-base) MAPSORT of: pF1KB8248.seq Check: 739 from: 1 to: 3818
pF1KB8248 3818 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 529 529
Size: 3818
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 834 834
Size: 3818
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1643 2075 2822 3090 1643
Size: 432 747 268 2371
Fragments arranged by size:
2371 747 432 268
AflIII A'CryG_T
Cuts at: 407 762 1170 2274 3251 407
Size: 355 408 1104 977 974
Fragments arranged by size:
1104 977 974 408 355
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 3818
AlwNI CAG_nnn'CTG
Cuts at: 216 718 735 1123 2690 216
Size: 502 17 388 1567 1344
Fragments arranged by size:
1567 1344 502 388 17
ApaLI G'TGCA_C
Cuts at: 2588 2588
Size: 3818
ApoI r'AATT_y
Cuts at: 822 3171 3427 822
Size: 2349 256 1213
Fragments arranged by size:
2349 1213 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 170 638 838 170
Size: 468 200 3150
Fragments arranged by size:
3150 468 200
BamHI G'GATC_C
Cuts at: 609 843 609
Size: 234 3584
BanI G'GyrC_C
Cuts at: 834 1248 1496 1531 834
Size: 414 248 35 3121
Fragments arranged by size:
3121 414 248 35
BanII G_rGCy'C
Cuts at: 558 707 725 832 1862 558
Size: 149 18 107 1030 2514
Fragments arranged by size:
2514 1030 149 107 18
BbeI G_GCGC'C
Cuts at: 1252 1500 1252
Size: 248 3570
BbsI GAAGACnn'nnnn_
Cuts at: 759 759
Size: 3818
BciVI GTATCCnnnnn_n'
Cuts at: 1716 2477 3818 1716
Size: 761 1341 1716
Fragments arranged by size:
1716 1341 761
BclI T'GATC_A
Cuts at: 398 1338 398
Size: 940 2878
BglI GCCn_nnn'nGGC
Cuts at: 523 523
Size: 3818
BglII A'GATC_T
Cuts at: 1333 3370 1333
Size: 2037 1781
BlpI GC'TnA_GC
Cuts at: 930 930
Size: 3818
Bme1580I G_kGCm'C
Cuts at: 433 548 1443 1536 2592 433
Size: 115 895 93 1056 1659
Fragments arranged by size:
1659 1056 895 115 93
BmgBI CAC'GTC
Cuts at: 765 765
Size: 3818
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 3818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 709
Size: 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3522 3522
Size: 3818
BsaAI yAC'GTr
Cuts at: 410 1171 410
Size: 761 3057
BsaBI GATnn'nnATC
Cuts at: 6 1332 3369 6
Size: 1326 2037 455
Fragments arranged by size:
2037 1326 455
BsaHI Gr'CG_yC
Cuts at: 131 1249 1497 2199 3695 131
Size: 1118 248 702 1496 254
Fragments arranged by size:
1496 1118 702 254 248
BsaWI w'CCGG_w
Cuts at: 1007 1528 2206 2480 2627 3038 1007
Size: 521 678 274 147 411 1787
Fragments arranged by size:
1787 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 130 127
Size: 3 3815
BseYI C'CCAG_C
Cuts at: 426 1243 2001 2578 3074 426
Size: 817 758 577 496 1170
Fragments arranged by size:
1170 817 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 293 1360 1406 2614 104
Size: 189 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 189 46
BsiHKAI G_wGCw'C
Cuts at: 725 832 1610 1800 2592 725
Size: 107 778 190 792 1951
Fragments arranged by size:
1951 792 778 190 107
BsmI GAATG_Cn'
Cuts at: 516 516
Size: 3818
BsmBI CGTCTCn'nnnn_
Cuts at: 112 112
Size: 3818
Bsp1286I G_dGCh'C
Cuts at: 433 548 558 707 725 832 1443 1536 1610
Size: 115 10 149 18 107 611 93 74
Cuts at: 1610 1800 1862 2592 433
Size: 190 62 730 1659
Fragments arranged by size:
1659 730 611 190 149 115 107 93
74 62 18 10
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 2994 2994
Size: 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 459 529 868 1082 1384 1765 224
Size: 235 70 339 214 302 381 2277
Fragments arranged by size:
2277 381 339 302 235 214 70
BsrBI CCG'CTC
Cuts at: 50 393 2110 2164 3648 3813 50
Size: 343 1717 54 1484 165 55
Fragments arranged by size:
1717 1484 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 1730
Size: 3818
BsrFI r'CCGG_y
Cuts at: 288 484 1816 1997 2206 288
Size: 196 1332 181 209 1900
Fragments arranged by size:
1900 1332 209 196 181
BssSI C'ACGA_G
Cuts at: 601 2089 2447 601
Size: 1488 358 1972
Fragments arranged by size:
1972 1488 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1195 1195
Size: 3818
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 3818
BstYI r'GATC_y
Cuts at: 1 609 843 1310 1333 1668 1914 2926 3012
Size: 608 234 467 23 335 246 1012 86
Cuts at: 3012 3024 3370 1
Size: 12 346 449
Fragments arranged by size:
1012 608 467 449 346 335 246 234
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 346 346
Size: 3818
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 3467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 494 1768 1949 2112 494
Size: 1274 181 163 2200
Fragments arranged by size:
2200 1274 181 163
BtsI GCAGTG_nn'
Cuts at: 736 1061 736
Size: 325 3493
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 265 1524 2382 265
Size: 1259 858 1701
Fragments arranged by size:
1701 1259 858
EaeI y'GGCC_r
Cuts at: 226 290 1403 1577 1968 1995 3340 226
Size: 64 1113 174 391 27 1345 704
Fragments arranged by size:
1345 1113 704 391 174 64 27
EagI C'GGCC_G
Cuts at: 290 1403 290
Size: 1113 2705
EarI CTCTTCn'nnn_
Cuts at: 376 565 1841 2051 376
Size: 189 1276 210 2143
Fragments arranged by size:
2143 1276 210 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2026 2336 2482 3628 2026
Size: 310 146 1146 2216
Fragments arranged by size:
2216 1146 310 146
EcoICRI GAG'CTC
Cuts at: 723 830 723
Size: 107 3711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1643 2075 2822 3090 709
Size: 934 432 747 268 1437
Fragments arranged by size:
1437 934 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 175 596 957 175
Size: 421 361 3036
Fragments arranged by size:
3036 421 361
EcoRI G'AATT_C
Cuts at: 822 822
Size: 3818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FseI GG_CCGG'CC
Cuts at: 292 292
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 1252 1500 2522 3501 1252
Size: 248 1022 979 1569
Fragments arranged by size:
1569 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 601 633 1660 1690 1692 1722 472
Size: 32 97 32 1027 30 2 30 2568
Fragments arranged by size:
2568 1027 97 32 32 30 30 2
HincII GTy'rAC
Cuts at: 857 857
Size: 3818
KasI G'GCGC_C
Cuts at: 1248 1496 1248
Size: 248 3570
KpnI G_GTAC'C
Cuts at: 838 838
Size: 3818
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2489 2673 2489
Size: 184 3634
MscI TGG'CCA
Cuts at: 1579 1579
Size: 3818
MslI CAynn'nnrTG
Cuts at: 457 460 1807 1934 457
Size: 3 1347 127 2341
Fragments arranged by size:
2341 1347 127 3
MspA1I CmG'CkG
Cuts at: 929 1243 1603 2616 2861 929
Size: 314 360 1013 245 1886
Fragments arranged by size:
1886 1013 360 314 245
NaeI GCC'GGC
Cuts at: 290 486 1999 290
Size: 196 1513 2109
Fragments arranged by size:
2109 1513 196
NarI GG'CG_CC
Cuts at: 1249 1497 1249
Size: 248 3570
NcoI C'CATG_G
Cuts at: 107 107
Size: 3818
NgoMIV G'CCGG_C
Cuts at: 288 484 1997 288
Size: 196 1513 2109
Fragments arranged by size:
2109 1513 196
NspI r_CATG'y
Cuts at: 871 1371 2278 871
Size: 500 907 2411
Fragments arranged by size:
2411 907 500
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PmlI CAC'GTG
Cuts at: 410 410
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3786
PpuMI rG'GwC_Cy
Cuts at: 175 596 175
Size: 421 3397
PstI C_TGCA'G
Cuts at: 187 470 794 865 1550 187
Size: 283 324 71 685 2455
Fragments arranged by size:
2455 685 324 283 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 1243 1603 1243
Size: 360 3458
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 3818
SacI G_AGCT'C
Cuts at: 725 832 725
Size: 107 3711
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SanDI GG'GwC_CC
Cuts at: 596 596
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 376 1841 2051 376
Size: 1465 210 2143
Fragments arranged by size:
2143 1465 210
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 183 466 790 861 1546 2539 2730 33
Size: 150 283 324 71 685 993 191 1121
Fragments arranged by size:
1121 993 685 324 283 191 150 71
SfoI GGC'GCC
Cuts at: 1250 1498 1250
Size: 248 3570
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SmaI CCC'GGG
Cuts at: 640 840 640
Size: 200 3618
SmlI C'TyrA_G
Cuts at: 973 1888 2380 2642 973
Size: 915 492 262 2149
Fragments arranged by size:
2149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
SrfI GCCC'GGGC
Cuts at: 640 640
Size: 3818
StyI C'CwwG_G
Cuts at: 107 698 952 1306 107
Size: 591 254 354 2619
Fragments arranged by size:
2619 591 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1401 1534 1993 2202 1401
Size: 133 459 209 3017
Fragments arranged by size:
3017 459 209 133
TatI w'GTAC_w
Cuts at: 3333 3333
Size: 3818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 3786
Tth111I GACn'n_nGTC
Cuts at: 1615 1615
Size: 3818
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XmaI C'CCGG_G
Cuts at: 638 838 638
Size: 200 3618
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsgI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI