Restriction map for
pF1KB8242
(Circular) (Six-base) MAPSORT of: pF1KB8242.seq Check: 1687 from: 1 to: 3707
pF1KB8242 3707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 467 745 467
Size: 278 3429
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 388 531 660 711 3060 3316 388
Size: 143 129 51 2349 256 779
Fragments arranged by size:
2349 779 256 143 129 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 117 727 117
Size: 610 3097
BamHI G'GATC_C
Cuts at: 204 732 204
Size: 528 3179
BanI G'GyrC_C
Cuts at: 130 723 1137 1385 1420 130
Size: 593 414 248 35 2417
Fragments arranged by size:
2417 593 414 248 35
BanII G_rGCy'C
Cuts at: 171 612 721 1751 171
Size: 441 109 1030 2127
Fragments arranged by size:
2127 1030 441 109
BbeI G_GCGC'C
Cuts at: 134 1141 1389 134
Size: 1007 248 2452
Fragments arranged by size:
2452 1007 248
BbsI GAAGACnn'nnnn_
Cuts at: 636 636
Size: 3707
BciVI GTATCCnnnnn_n'
Cuts at: 298 630 1605 2366 3707 298
Size: 332 975 761 1341 298
Fragments arranged by size:
1341 975 761 332 298
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 644 819 644
Size: 175 3532
Bme1580I G_kGCm'C
Cuts at: 1332 1425 2481 1332
Size: 93 1056 2558
Fragments arranged by size:
2558 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 343 441 320
Size: 23 98 3586
Fragments arranged by size:
3586 98 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 131 1138 1386 2088 3584 131
Size: 1007 248 702 1496 254
Fragments arranged by size:
1496 1007 702 254 248
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1132 1890 2467 2963 1132
Size: 758 577 496 1876
Fragments arranged by size:
1876 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 721 1499 1689 2481 721
Size: 778 190 792 1947
Fragments arranged by size:
1947 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 171 612 721 1332 1425 1499 1689 1751 2481
Size: 441 109 611 93 74 190 62 730
Cuts at: 2481 171
Size: 1397
Fragments arranged by size:
1397 730 611 441 190 109 93 74
62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 692 2883 692
Size: 2191 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 971 1273 1654 757
Size: 214 302 381 2810
Fragments arranged by size:
2810 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 1619 293
Size: 1326 2381
BsrFI r'CCGG_y
Cuts at: 133 332 1705 1886 2095 133
Size: 199 1373 181 209 1745
Fragments arranged by size:
1745 1373 209 199 181
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1084
Size: 3707
BstBI TT'CG_AA
Cuts at: 627 2068 627
Size: 1441 2266
BstYI r'GATC_y
Cuts at: 1 204 732 1199 1222 1557 1803 2815 2901
Size: 203 528 467 23 335 246 1012 86
Cuts at: 2901 2913 3259 1
Size: 12 346 449
Fragments arranged by size:
1012 528 467 449 346 335 246 203
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 570 1657 1838 2001 570
Size: 1087 181 163 2276
Fragments arranged by size:
2276 1087 181 163
BtsI GCAGTG_nn'
Cuts at: 950 950
Size: 3707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 461 496 461
Size: 35 3672
DraI TTT'AAA
Cuts at: 393 706 393
Size: 313 3394
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 343 441 1532 1964 2711 2979 320
Size: 23 98 1091 432 747 268 1048
Fragments arranged by size:
1091 1048 747 432 268 98 23
EcoO109I rG'GnC_Cy
Cuts at: 178 489 846 178
Size: 311 357 3039
Fragments arranged by size:
3039 357 311
EcoRI G'AATT_C
Cuts at: 531 711 531
Size: 180 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 588 1726 588
Size: 1138 2569
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 134 1141 1389 2411 3390 134
Size: 1007 248 1022 979 451
Fragments arranged by size:
1022 1007 979 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1579 1581 1611 1549
Size: 30 2 30 3645
Fragments arranged by size:
3645 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 130 1137 1385 130
Size: 1007 248 2452
Fragments arranged by size:
2452 1007 248
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MfeI C'AATT_G
Cuts at: 536 536
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2562 2378
Size: 184 3523
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 1696 1823 1696
Size: 127 3580
MspA1I CmG'CkG
Cuts at: 210 818 1132 1492 2505 2750 210
Size: 608 314 360 1013 245 1167
Fragments arranged by size:
1167 1013 608 360 314 245
NaeI GCC'GGC
Cuts at: 135 334 1888 135
Size: 199 1554 1954
Fragments arranged by size:
1954 1554 199
NarI GG'CG_CC
Cuts at: 131 1138 1386 131
Size: 1007 248 2452
Fragments arranged by size:
2452 1007 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3707
NdeI CA'TA_TG
Cuts at: 669 669
Size: 3707
NgoMIV G'CCGG_C
Cuts at: 133 332 1886 133
Size: 199 1554 1954
Fragments arranged by size:
1954 1554 199
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PfoI T'CCnGG_A
Cuts at: 171 189 171
Size: 18 3689
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PstI C_TGCA'G
Cuts at: 754 1439 754
Size: 685 3022
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 230 750 1435 2428 2619 33
Size: 197 520 685 993 191 1121
Fragments arranged by size:
1121 993 685 520 197 191
SfoI GGC'GCC
Cuts at: 132 1139 1387 132
Size: 1007 248 2452
Fragments arranged by size:
2452 1007 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SgrAI Cr'CCGG_yG
Cuts at: 133 133
Size: 3707
SmaI CCC'GGG
Cuts at: 119 729 119
Size: 610 3097
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 107 841 1195 107
Size: 734 354 2619
Fragments arranged by size:
2619 734 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 408 3222 408
Size: 2814 893
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 285 1504 285
Size: 1219 2488
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 117 727 117
Size: 610 3097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI