Restriction map for
pF1KB8238
(Circular) (Six-base) MAPSORT of: pF1KB8238.seq Check: 9138 from: 1 to: 4004
pF1KB8238 4004 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 678 678
Size: 4004
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 607 697 1829 2261 3008 3276 430
Size: 177 90 1132 432 747 268 1158
Fragments arranged by size:
1158 1132 747 432 268 177 90
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AleI CACnn'nnGTG
Cuts at: 688 688
Size: 4004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 553 553
Size: 4004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 439 471 211
Size: 32 196 32 3744
Fragments arranged by size:
3744 196 32 32
AlwNI CAG_nnn'CTG
Cuts at: 354 363 636 1309 2876 354
Size: 9 273 673 1567 1482
Fragments arranged by size:
1567 1482 673 273 9
ApaLI G'TGCA_C
Cuts at: 187 2774 187
Size: 2587 1417
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 192 331 1024 192
Size: 139 693 3172
Fragments arranged by size:
3172 693 139
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 1020 1434 1682 1717 1020
Size: 414 248 35 3307
Fragments arranged by size:
3307 414 248 35
BanII G_rGCy'C
Cuts at: 217 425 518 850 1018 2048 217
Size: 208 93 332 168 1030 2173
Fragments arranged by size:
2173 1030 332 208 168 93
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 676 710 90
Size: 34 552 34 3384
Fragments arranged by size:
3384 552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 818 1524 818
Size: 706 3298
BglII A'GATC_T
Cuts at: 713 1519 3556 713
Size: 806 2037 1161
Fragments arranged by size:
2037 1161 806
BlpI GC'TnA_GC
Cuts at: 849 1116 849
Size: 267 3737
Bme1580I G_kGCm'C
Cuts at: 191 199 1629 1722 2778 191
Size: 8 1430 93 1056 1417
Fragments arranged by size:
1430 1417 1056 93 8
BmgBI CAC'GTC
Cuts at: 882 882
Size: 4004
BmrI ACTGGGnnnn_n'
Cuts at: 280 1216 1297 1632 3776 280
Size: 936 81 335 2144 508
Fragments arranged by size:
2144 936 508 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 916
Size: 4004
Bpu10I CC'TnA_GC
Cuts at: 844 844
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 861 1357 861
Size: 496 3508
BsaBI GATnn'nnATC
Cuts at: 6 937 1518 3555 6
Size: 931 581 2037 455
Fragments arranged by size:
2037 931 581 455
BsaHI Gr'CG_yC
Cuts at: 983 1435 1683 2385 3881 983
Size: 452 248 702 1496 1106
Fragments arranged by size:
1496 1106 702 452 248
BsaWI w'CCGG_w
Cuts at: 242 1193 1714 2392 2666 2813 3224 242
Size: 951 521 678 274 147 411 1022
Fragments arranged by size:
1022 951 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 439 469 439
Size: 30 3974
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 349 634 238
Size: 111 285 3608
Fragments arranged by size:
3608 285 111
BseYI C'CCAG_C
Cuts at: 207 384 482 500 601 1429 2187 2764 3260
Size: 177 98 18 101 828 758 577 496
Cuts at: 3260 207
Size: 951
Fragments arranged by size:
951 828 758 577 496 177 101 98
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 751
Size: 4004
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 518 850 881 1018 1796 1986 2778 191
Size: 327 332 31 137 778 190 792 1417
Fragments arranged by size:
1417 792 778 332 327 190 137 31
Bsp1286I G_dGCh'C
Cuts at: 191 199 217 425 518 850 881 1018 1629
Size: 8 18 208 93 332 31 137 611
Cuts at: 1629 1722 1796 1986 2048 2778 191
Size: 93 74 190 62 730 1417
Fragments arranged by size:
1417 730 611 332 208 190 137 93
93 74 62 31 18 8
BspEI T'CCGG_A
Cuts at: 242 3224 242
Size: 2982 1022
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 294 371 678 1054 1268 1570 1951 294
Size: 77 307 376 214 302 381 2347
Fragments arranged by size:
2347 381 376 307 302 214 77
BsrBI CCG'CTC
Cuts at: 50 171 570 2296 2350 3834 3999 50
Size: 121 399 1726 54 1484 165 55
Fragments arranged by size:
1726 1484 399 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssSI C'ACGA_G
Cuts at: 124 2275 2633 124
Size: 2151 358 1495
Fragments arranged by size:
2151 1495 358
BstAPI GCAn_nnn'nTGC
Cuts at: 354 363 1381 354
Size: 9 1018 2977
Fragments arranged by size:
2977 1018 9
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstEII G'GTnAC_C
Cuts at: 306 306
Size: 4004
BstYI r'GATC_y
Cuts at: 1 395 713 734 1029 1496 1519 1854 2100
Size: 394 318 21 295 467 23 335 246
Cuts at: 2100 3112 3198 3210 3556 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 394 346 335 318 295
246 86 23 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 383 1954 2135 2298 383
Size: 1571 181 163 2089
Fragments arranged by size:
2089 1571 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1247
Size: 4004
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 317 1589 1763 2154 2181 3526 317
Size: 1272 174 391 27 1345 795
Fragments arranged by size:
1345 1272 795 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 152 2027 2237 152
Size: 1875 210 1919
Fragments arranged by size:
1919 1875 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 388 2212 2522 2668 3814 388
Size: 1824 310 146 1146 578
Fragments arranged by size:
1824 1146 578 310 146
EcoICRI GAG'CTC
Cuts at: 516 848 1016 516
Size: 332 168 3504
Fragments arranged by size:
3504 332 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 607 697 916 1829 2261 3008 3276 430
Size: 177 90 219 913 432 747 268 1158
Fragments arranged by size:
1158 913 747 432 268 219 177 90
EcoNI CCTnn'n_nnAGG
Cuts at: 331 331
Size: 4004
EcoO109I rG'GnC_Cy
Cuts at: 119 492 1143 119
Size: 373 651 2980
Fragments arranged by size:
2980 651 373
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 945 2023 945
Size: 1078 2926
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 807 839 1846 1876 1878 1908 133
Size: 32 642 32 1007 30 2 30 2229
Fragments arranged by size:
2229 1007 642 32 32 30 30 2
HincII GTy'rAC
Cuts at: 462 1043 462
Size: 581 3423
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 272 605 975 2675 2859 272
Size: 333 370 1700 184 1417
Fragments arranged by size:
1700 1417 370 333 184
MscI TGG'CCA
Cuts at: 319 1765 319
Size: 1446 2558
MslI CAynn'nnrTG
Cuts at: 469 688 943 1993 2120 469
Size: 219 255 1050 127 2353
Fragments arranged by size:
2353 1050 255 219 127
MspA1I CmG'CkG
Cuts at: 384 408 585 853 1115 1429 1789 2802 3047
Size: 24 177 268 262 314 360 1013 245
Cuts at: 3047 384
Size: 1341
Fragments arranged by size:
1341 1013 360 314 268 262 245 177
24
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 1057 1557 2464 1057
Size: 500 907 2597
Fragments arranged by size:
2597 907 500
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PflMI CCAn_nnn'nTGG
Cuts at: 291 291
Size: 4004
PfoI T'CCnGG_A
Cuts at: 284 284
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 439 471 3143 3175 439
Size: 32 2672 32 1268
Fragments arranged by size:
2672 1268 32 32
PpuMI rG'GwC_Cy
Cuts at: 119 492 119
Size: 373 3631
PshAI GACnn'nnGTC
Cuts at: 262 262
Size: 4004
PstI C_TGCA'G
Cuts at: 359 407 1051 1736 359
Size: 48 644 685 2627
Fragments arranged by size:
2627 685 644 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 384 408 585 1429 1789 384
Size: 24 177 844 360 2599
Fragments arranged by size:
2599 844 360 177 24
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 518 850 1018 518
Size: 332 168 3504
Fragments arranged by size:
3504 332 168
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 355 403 1047 1732 2725 2916 33
Size: 322 48 644 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 322 191 48
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 194 1026 194
Size: 832 3172
SmlI C'TyrA_G
Cuts at: 1159 2074 2566 2828 1159
Size: 915 492 262 2335
Fragments arranged by size:
2335 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StuI AGG'CCT
Cuts at: 703 703
Size: 4004
StyI C'CwwG_G
Cuts at: 1138 1492 1138
Size: 354 3650
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 312 344 3521 3553 312
Size: 32 3177 32 763
Fragments arranged by size:
3177 763 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 603 1499 603
Size: 896 3108
XmaI C'CCGG_G
Cuts at: 192 1024 192
Size: 832 3172
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI