Restriction map for
pF1KB8236
(Circular) (Six-base) MAPSORT of: pF1KB8236.seq Check: 1500 from: 1 to: 3995
pF1KB8236 3995 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1033 1033
Size: 3995
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1820 2252 2999 3267 686
Size: 1134 432 747 268 1414
Fragments arranged by size:
1414 1134 747 432 268
AflII C'TTAA_G
Cuts at: 405 513 405
Size: 108 3887
AflIII A'CryG_T
Cuts at: 1347 2451 3428 1347
Size: 1104 977 1914
Fragments arranged by size:
1914 1104 977
AgeI A'CCGG_T
Cuts at: 2383 2383
Size: 3995
AlwNI CAG_nnn'CTG
Cuts at: 1300 2867 1300
Size: 1567 2428
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 907 999 3348 3604 907
Size: 92 2349 256 1298
Fragments arranged by size:
2349 1298 256 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 1015 1015
Size: 3995
BamHI G'GATC_C
Cuts at: 1020 1020
Size: 3995
BanI G'GyrC_C
Cuts at: 1011 1425 1673 1708 1011
Size: 414 248 35 3298
Fragments arranged by size:
3298 414 248 35
BanII G_rGCy'C
Cuts at: 139 1009 2039 139
Size: 870 1030 2095
Fragments arranged by size:
2095 1030 870
BbeI G_GCGC'C
Cuts at: 1429 1677 1429
Size: 248 3747
BbsI GAAGACnn'nnnn_
Cuts at: 848 848
Size: 3995
BciVI GTATCCnnnnn_n'
Cuts at: 1893 2654 3995 1893
Size: 761 1341 1893
Fragments arranged by size:
1893 1341 761
BclI T'GATC_A
Cuts at: 1515 1515
Size: 3995
BglII A'GATC_T
Cuts at: 963 1510 3547 963
Size: 547 2037 1411
Fragments arranged by size:
2037 1411 547
BlpI GC'TnA_GC
Cuts at: 1107 1107
Size: 3995
Bme1580I G_kGCm'C
Cuts at: 1620 1713 2769 1620
Size: 93 1056 2846
Fragments arranged by size:
2846 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1207 1288 1623 3767 1207
Size: 81 335 2144 1435
Fragments arranged by size:
2144 1435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1001 2362 1001
Size: 1361 2634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 689 563
Size: 126 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2050 2542 2840 1171
Size: 879 492 298 2326
Fragments arranged by size:
2326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3699 3699
Size: 3995
BsaAI yAC'GTr
Cuts at: 1348 1348
Size: 3995
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 1426 1674 2376 3872 1426
Size: 248 702 1496 1549
Fragments arranged by size:
1549 1496 702 248
BsaWI w'CCGG_w
Cuts at: 935 1184 1705 2383 2657 2804 3215 935
Size: 249 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 249 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 511 508
Size: 3 3992
BseYI C'CCAG_C
Cuts at: 1420 2178 2755 3251 1420
Size: 758 577 496 2164
Fragments arranged by size:
2164 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1537 1583 2791 104
Size: 1433 46 1208 1308
Fragments arranged by size:
1433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 227 1009 1787 1977 2769 227
Size: 782 778 190 792 1453
Fragments arranged by size:
1453 792 782 778 190
Bsp1286I G_dGCh'C
Cuts at: 139 227 1009 1620 1713 1787 1977 2039 2769
Size: 88 782 611 93 74 190 62 730
Cuts at: 2769 139
Size: 1365
Fragments arranged by size:
1365 782 730 611 190 93 88 74
62
BspEI T'CCGG_A
Cuts at: 935 3215 935
Size: 2280 1715
BspHI T'CATG_A
Cuts at: 3171 3171
Size: 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1045 1259 1561 1942 1045
Size: 214 302 381 3098
Fragments arranged by size:
3098 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2287 2341 3825 3990 50
Size: 2237 54 1484 165 55
Fragments arranged by size:
2237 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 305 1907 305
Size: 1602 2393
BsrFI r'CCGG_y
Cuts at: 1993 2174 2383 1993
Size: 181 209 3605
Fragments arranged by size:
3605 209 181
BsrGI T'GTAC_A
Cuts at: 354 354
Size: 3995
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 1372 1372
Size: 3995
BstBI TT'CG_AA
Cuts at: 2356 2356
Size: 3995
BstYI r'GATC_y
Cuts at: 1 963 1020 1487 1510 1845 2091 3103 3189
Size: 962 57 467 23 335 246 1012 86
Cuts at: 3189 3201 3547 1
Size: 12 346 449
Fragments arranged by size:
1012 962 467 449 346 335 246 86
57 23 12
Bsu36I CC'TnA_GG
Cuts at: 142 142
Size: 3995
BtgI C'CryG_G
Cuts at: 107 107
Size: 3995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 979 1238 979
Size: 259 3736
DraI TTT'AAA
Cuts at: 994 994
Size: 3995
DrdI GACnn_nn'nnGTC
Cuts at: 1701 2559 1701
Size: 858 3137
EaeI y'GGCC_r
Cuts at: 1580 1754 2145 2172 3517 1580
Size: 174 391 27 1345 2058
Fragments arranged by size:
2058 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1580 1580
Size: 3995
EarI CTCTTCn'nnn_
Cuts at: 485 608 893 2018 2228 485
Size: 123 285 1125 210 2252
Fragments arranged by size:
2252 1125 285 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2203 2513 2659 3805 2203
Size: 310 146 1146 2393
Fragments arranged by size:
2393 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1007 1007
Size: 3995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 686 689 1820 2252 2999 3267 563
Size: 123 3 1131 432 747 268 1291
Fragments arranged by size:
1291 1131 747 432 268 123 3
EcoO109I rG'GnC_Cy
Cuts at: 146 826 1134 146
Size: 680 308 3007
Fragments arranged by size:
3007 680 308
EcoRI G'AATT_C
Cuts at: 999 999
Size: 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 595 948 2014 595
Size: 353 1066 2576
Fragments arranged by size:
2576 1066 353
FspI TGC'GCA
Cuts at: 1776 1776
Size: 3995
HaeII r_GCGC'y
Cuts at: 679 1429 1677 2699 3678 679
Size: 750 248 1022 979 996
Fragments arranged by size:
1022 996 979 750 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 463 495 1837 1867 1869 1899 463
Size: 32 1342 30 2 30 2559
Fragments arranged by size:
2559 1342 32 30 30 2
HincII GTy'rAC
Cuts at: 1034 1034
Size: 3995
KasI G'GCGC_C
Cuts at: 1425 1673 1425
Size: 248 3747
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2666 2850 2666
Size: 184 3811
MscI TGG'CCA
Cuts at: 1756 1756
Size: 3995
MslI CAynn'nnrTG
Cuts at: 130 440 1984 2111 130
Size: 310 1544 127 2014
Fragments arranged by size:
2014 1544 310 127
MspA1I CmG'CkG
Cuts at: 1106 1420 1780 2793 3038 1106
Size: 314 360 1013 245 2063
Fragments arranged by size:
2063 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 3995
NarI GG'CG_CC
Cuts at: 1426 1674 1426
Size: 248 3747
NcoI C'CATG_G
Cuts at: 107 107
Size: 3995
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 3995
NspI r_CATG'y
Cuts at: 1048 1548 2455 1048
Size: 500 907 2588
Fragments arranged by size:
2588 907 500
PciI A'CATG_T
Cuts at: 2451 2451
Size: 3995
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PpuMI rG'GwC_Cy
Cuts at: 826 826
Size: 3995
PstI C_TGCA'G
Cuts at: 1042 1727 1042
Size: 685 3310
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 1420 1780 1420
Size: 360 3635
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 1009 1009
Size: 3995
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
SbfI CC_TGCA'GG
Cuts at: 1042 1042
Size: 3995
ScaI AGT'ACT
Cuts at: 3512 3512
Size: 3995
SfcI C'TryA_G
Cuts at: 33 836 1038 1723 2716 2907 33
Size: 803 202 685 993 191 1121
Fragments arranged by size:
1121 993 803 685 202 191
SfoI GGC'GCC
Cuts at: 1427 1675 1427
Size: 248 3747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 1017 1017
Size: 3995
SmlI C'TyrA_G
Cuts at: 405 513 1150 2065 2557 2819 405
Size: 108 637 915 492 262 1581
Fragments arranged by size:
1581 915 637 492 262 108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 1048 1548 1048
Size: 500 3495
StyI C'CwwG_G
Cuts at: 107 372 1129 1483 107
Size: 265 757 354 2619
Fragments arranged by size:
2619 757 354 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1578 1711 2170 2379 1578
Size: 133 459 209 3194
Fragments arranged by size:
3194 459 209 133
TatI w'GTAC_w
Cuts at: 354 3510 354
Size: 3156 839
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 3963
Tth111I GACn'n_nGTC
Cuts at: 1792 1792
Size: 3995
XbaI T'CTAG_A
Cuts at: 1026 1026
Size: 3995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1490 1490
Size: 3995
XmaI C'CCGG_G
Cuts at: 1015 1015
Size: 3995
XmnI GAAnn'nnTTC
Cuts at: 931 931
Size: 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI