Restriction map for
pF1KB8235
(Circular) (Six-base) MAPSORT of: pF1KB8235.seq Check: 9504 from: 1 to: 3773
pF1KB8235 3773 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 729 729
Size: 3773
AatII G_ACGT'C
Cuts at: 217 217
Size: 3773
AccI GT'mk_AC
Cuts at: 811 811
Size: 3773
Acc65I G'GTAC_C
Cuts at: 356 789 356
Size: 433 3340
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1598 2030 2777 3045 1598
Size: 432 747 268 2326
Fragments arranged by size:
2326 747 432 268
AflIII A'CryG_T
Cuts at: 170 1125 2229 3206 170
Size: 955 1104 977 737
Fragments arranged by size:
1104 977 955 737
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 498 1078 2645 498
Size: 580 1567 1626
Fragments arranged by size:
1626 1567 580
ApaI G_GGCC'C
Cuts at: 721 721
Size: 3773
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 777 3126 3382 777
Size: 2349 256 1168
Fragments arranged by size:
2349 1168 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 793 793
Size: 3773
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 356 706 741 789 1203 1451 1486 356
Size: 350 35 48 414 248 35 2643
Fragments arranged by size:
2643 414 350 248 48 35 35
BanII G_rGCy'C
Cuts at: 721 787 1817 721
Size: 66 1030 2677
Fragments arranged by size:
2677 1030 66
BbeI G_GCGC'C
Cuts at: 710 1207 1455 710
Size: 497 248 3028
Fragments arranged by size:
3028 497 248
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglII A'GATC_T
Cuts at: 329 1288 3325 329
Size: 959 2037 777
Fragments arranged by size:
2037 959 777
BlpI GC'TnA_GC
Cuts at: 885 885
Size: 3773
Bme1580I G_kGCm'C
Cuts at: 721 1398 1491 2547 721
Size: 677 93 1056 1947
Fragments arranged by size:
1947 1056 677 93
BmrI ACTGGGnnnn_n'
Cuts at: 985 1066 1401 3545 985
Size: 81 335 2144 1213
Fragments arranged by size:
2144 1213 335 81
BmtI G_CTAG'C
Cuts at: 568 568
Size: 3773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1828 2320 2618 949
Size: 879 492 298 2104
Fragments arranged by size:
2104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3477 3477
Size: 3773
BsaAI yAC'GTr
Cuts at: 1126 1126
Size: 3773
BsaBI GATnn'nnATC
Cuts at: 6 184 1287 3324 6
Size: 178 1103 2037 455
Fragments arranged by size:
2037 1103 455 178
BsaHI Gr'CG_yC
Cuts at: 214 707 1204 1452 2154 3650 214
Size: 493 497 248 702 1496 337
Fragments arranged by size:
1496 702 497 493 337 248
BsaWI w'CCGG_w
Cuts at: 359 962 1483 2161 2435 2582 2993 359
Size: 603 521 678 274 147 411 1139
Fragments arranged by size:
1139 678 603 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1198 1956 2533 3029 1198
Size: 758 577 496 1942
Fragments arranged by size:
1942 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 466
Size: 3773
BsiEI CG_ry'CG
Cuts at: 104 1315 1361 2569 104
Size: 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46
BsiHKAI G_wGCw'C
Cuts at: 787 1565 1755 2547 787
Size: 778 190 792 2013
Fragments arranged by size:
2013 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 721 787 1398 1491 1565 1755 1817 2547 721
Size: 66 611 93 74 190 62 730 1947
Fragments arranged by size:
1947 730 611 190 93 74 66 62
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 729 823 1037 1339 1720 729
Size: 94 214 302 381 2782
Fragments arranged by size:
2782 381 302 214 94
BsrBI CCG'CTC
Cuts at: 50 354 534 2065 2119 3603 3768 50
Size: 304 180 1531 54 1484 165 55
Fragments arranged by size:
1531 1484 304 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1685 1685
Size: 3773
BsrFI r'CCGG_y
Cuts at: 557 1771 1952 2161 557
Size: 1214 181 209 2169
Fragments arranged by size:
2169 1214 209 181
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 2134 2134
Size: 3773
BstYI r'GATC_y
Cuts at: 1 329 798 1265 1288 1623 1869 2881 2967
Size: 328 469 467 23 335 246 1012 86
Cuts at: 2967 2979 3325 1
Size: 12 346 449
Fragments arranged by size:
1012 469 467 449 346 335 328 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 269 107
Size: 162 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 449 1016 449
Size: 567 3206
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 286 697 1479 2337 286
Size: 411 782 858 1722
Fragments arranged by size:
1722 858 782 411
EaeI y'GGCC_r
Cuts at: 555 1358 1532 1923 1950 3295 555
Size: 803 174 391 27 1345 1033
Fragments arranged by size:
1345 1033 803 391 174 27
EagI C'GGCC_G
Cuts at: 1358 1358
Size: 3773
EarI CTCTTCn'nnn_
Cuts at: 229 1796 2006 229
Size: 1567 210 1996
Fragments arranged by size:
1996 1567 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 105 703 1981 2291 2437 3583 105
Size: 598 1278 310 146 1146 295
Fragments arranged by size:
1278 1146 598 310 295 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1598 2030 2777 3045 1598
Size: 432 747 268 2326
Fragments arranged by size:
2326 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 717 912 717
Size: 195 3578
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 234 648 1792 234
Size: 414 1144 2215
Fragments arranged by size:
2215 1144 414
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 392 605 642 710 1207 1455 2477 3456 392
Size: 213 37 68 497 248 1022 979 709
Fragments arranged by size:
1022 979 709 497 248 213 68 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 652 684 1615 1645 1647 1677 285
Size: 32 335 32 931 30 2 30 2381
Fragments arranged by size:
2381 931 335 32 32 30 30 2
HincII GTy'rAC
Cuts at: 812 812
Size: 3773
KasI G'GCGC_C
Cuts at: 706 1203 1451 706
Size: 497 248 3028
Fragments arranged by size:
3028 497 248
KpnI G_GTAC'C
Cuts at: 360 793 360
Size: 433 3340
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2444 2628 659
Size: 1785 184 1804
Fragments arranged by size:
1804 1785 184
MscI TGG'CCA
Cuts at: 1534 1534
Size: 3773
MslI CAynn'nnrTG
Cuts at: 406 1762 1889 406
Size: 1356 127 2290
Fragments arranged by size:
2290 1356 127
MspA1I CmG'CkG
Cuts at: 884 1198 1558 2571 2816 884
Size: 314 360 1013 245 1841
Fragments arranged by size:
1841 1013 360 314 245
NaeI GCC'GGC
Cuts at: 559 1954 559
Size: 1395 2378
NarI GG'CG_CC
Cuts at: 707 1204 1452 707
Size: 497 248 3028
Fragments arranged by size:
3028 497 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 557 1952 557
Size: 1395 2378
NheI G'CTAG_C
Cuts at: 564 564
Size: 3773
NspI r_CATG'y
Cuts at: 174 826 1326 2233 174
Size: 652 500 907 1714
Fragments arranged by size:
1714 907 652 500
PciI A'CATG_T
Cuts at: 170 2229 170
Size: 2059 1714
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PspOMI G'GGCC_C
Cuts at: 717 717
Size: 3773
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 151 183 151
Size: 32 3741
PstI C_TGCA'G
Cuts at: 329 820 1505 329
Size: 491 685 2597
Fragments arranged by size:
2597 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SalI G'TCGA_C
Cuts at: 810 810
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 325 816 1501 2494 2685 33
Size: 292 491 685 993 191 1121
Fragments arranged by size:
1121 993 685 491 292 191
SfoI GGC'GCC
Cuts at: 708 1205 1453 708
Size: 497 248 3028
Fragments arranged by size:
3028 497 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 795 795
Size: 3773
SmlI C'TyrA_G
Cuts at: 928 1843 2335 2597 928
Size: 915 492 262 2104
Fragments arranged by size:
2104 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
StyI C'CwwG_G
Cuts at: 107 576 605 907 1261 107
Size: 469 29 302 354 2619
Fragments arranged by size:
2619 469 354 302 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 475 1356 1489 1948 2157 475
Size: 881 133 459 209 2091
Fragments arranged by size:
2091 881 459 209 133
TatI w'GTAC_w
Cuts at: 3288 3288
Size: 3773
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1268 1268
Size: 3773
XmaI C'CCGG_G
Cuts at: 793 793
Size: 3773
ZraI GAC'GTC
Cuts at: 215 215
Size: 3773
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI