Restriction map for
pF1KB8234
(Circular) (Six-base) MAPSORT of: pF1KB8234.seq Check: 2534 from: 1 to: 3887
pF1KB8234 3887 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 704 903 704
Size: 199 3688
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 1712 2144 2891 3159 621
Size: 1091 432 747 268 1349
Fragments arranged by size:
1349 1091 747 432 268
AflIII A'CryG_T
Cuts at: 385 1239 2343 3320 385
Size: 854 1104 977 952
Fragments arranged by size:
1104 977 952 854
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AleI CACnn'nnGTG
Cuts at: 353 353
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 1192 2759 1192
Size: 1567 2320
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 907 907
Size: 3887
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 486 519 624 657 486
Size: 33 105 33 3716
Fragments arranged by size:
3716 105 33 33
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 704 903 1317 1565 1600 704
Size: 199 414 248 35 2991
Fragments arranged by size:
2991 414 248 199 35
BanII G_rGCy'C
Cuts at: 272 901 1931 272
Size: 629 1030 2228
Fragments arranged by size:
2228 1030 629
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BbsI GAAGACnn'nnnn_
Cuts at: 388 388
Size: 3887
BbvCI CC'TCA_GC
Cuts at: 136 136
Size: 3887
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 1512 1605 2661 1512
Size: 93 1056 2738
Fragments arranged by size:
2738 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 863 1099 1180 1515 3659 863
Size: 236 81 335 2144 1091
Fragments arranged by size:
2144 1091 335 236 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 683 2254 683
Size: 1571 2316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 178
Size: 3887
Bpu10I CC'TnA_GC
Cuts at: 136 136
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 595 693 3591 595
Size: 98 2898 891
Fragments arranged by size:
2898 891 98
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 115 1318 1566 2268 3764 115
Size: 1203 248 702 1496 238
Fragments arranged by size:
1496 1203 702 248 238
BsaWI w'CCGG_w
Cuts at: 732 1076 1597 2275 2549 2696 3107 732
Size: 344 521 678 274 147 411 1512
Fragments arranged by size:
1512 678 521 411 344 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 521 551 198
Size: 30 293 30 3534
Fragments arranged by size:
3534 293 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 837 583
Size: 254 3633
BseYI C'CCAG_C
Cuts at: 1312 2070 2647 3143 1312
Size: 758 577 496 2056
Fragments arranged by size:
2056 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 879 901 1679 1869 2661 879
Size: 22 778 190 792 2105
Fragments arranged by size:
2105 792 778 190 22
Bsp1286I G_dGCh'C
Cuts at: 272 879 901 1512 1605 1679 1869 1931 2661
Size: 607 22 611 93 74 190 62 730
Cuts at: 2661 272
Size: 1498
Fragments arranged by size:
1498 730 611 607 190 93 74 62
22
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 937 1151 1453 1834 937
Size: 214 302 381 2990
Fragments arranged by size:
2990 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 758 1885 2066 2275 758
Size: 1127 181 209 2370
Fragments arranged by size:
2370 1127 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstEII G'GTnAC_C
Cuts at: 694 694
Size: 3887
BstXI CCAn_nnnn'nTGG
Cuts at: 355 355
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 622 709 107
Size: 515 87 3285
Fragments arranged by size:
3285 515 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 1472 1646 2037 2064 3409 1472
Size: 174 391 27 1345 1950
Fragments arranged by size:
1950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 166 779 1910 2120 166
Size: 613 1131 210 1933
Fragments arranged by size:
1933 1131 613 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 659 2095 2405 2551 3697 659
Size: 1436 310 146 1146 849
Fragments arranged by size:
1436 1146 849 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 621 1712 2144 2891 3159 178
Size: 443 1091 432 747 268 906
Fragments arranged by size:
1091 906 747 443 432 268
EcoO109I rG'GnC_Cy
Cuts at: 627 865 1026 627
Size: 238 161 3488
Fragments arranged by size:
3488 238 161
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 1321 1569 2591 3570 1321
Size: 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 852 884 1729 1759 1761 1791 220
Size: 32 600 32 845 30 2 30 2316
Fragments arranged by size:
2316 845 600 32 32 30 30 2
HincII GTy'rAC
Cuts at: 453 557 926 453
Size: 104 369 3414
Fragments arranged by size:
3414 369 104
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 708 907 708
Size: 199 3688
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 376 2558 2742 376
Size: 2182 184 1521
Fragments arranged by size:
2182 1521 184
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 353 1876 2003 353
Size: 1523 127 2237
Fragments arranged by size:
2237 1523 127
MspA1I CmG'CkG
Cuts at: 372 838 998 1312 1672 2685 2930 372
Size: 466 160 314 360 1013 245 1329
Fragments arranged by size:
1329 1013 466 360 314 245 160
NaeI GCC'GGC
Cuts at: 760 2068 760
Size: 1308 2579
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NcoI C'CATG_G
Cuts at: 107 709 107
Size: 602 3285
NgoMIV G'CCGG_C
Cuts at: 758 2066 758
Size: 1308 2579
NspI r_CATG'y
Cuts at: 389 451 940 1440 2347 389
Size: 62 489 500 907 1929
Fragments arranged by size:
1929 907 500 489 62
PciI A'CATG_T
Cuts at: 385 2343 385
Size: 1958 1929
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 170 202 3026 3058 170
Size: 32 2824 32 999
Fragments arranged by size:
2824 999 32 32
PpuMI rG'GwC_Cy
Cuts at: 627 627
Size: 3887
PsiI TTA'TAA
Cuts at: 260 260
Size: 3887
PstI C_TGCA'G
Cuts at: 934 1619 934
Size: 685 3202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 1312 1672 1312
Size: 360 3527
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 930 1615 2608 2799 33
Size: 897 685 993 191 1121
Fragments arranged by size:
1121 993 897 685 191
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 107 709 1021 1375 107
Size: 602 312 354 2619
Fragments arranged by size:
2619 602 354 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1470 1603 2062 2271 1470
Size: 133 459 209 3086
Fragments arranged by size:
3086 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 521 553 3404 3436 198
Size: 32 291 32 2851 32 649
Fragments arranged by size:
2851 649 291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 307 716 787 1382 307
Size: 409 71 595 2812
Fragments arranged by size:
2812 595 409 71
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI