Restriction map for
pF1KB8228
(Circular) (Six-base) MAPSORT of: pF1KB8228.seq Check: 7064 from: 1 to: 4340
pF1KB8228 4340 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1378 1378
Size: 4340
Acc65I G'GTAC_C
Cuts at: 1356 1356
Size: 4340
AclI AA'CG_TT
Cuts at: 4180 4180
Size: 4340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2597 3344 3612 2165
Size: 432 747 268 2893
Fragments arranged by size:
2893 747 432 268
AflIII A'CryG_T
Cuts at: 1692 2796 3773 1692
Size: 1104 977 2259
Fragments arranged by size:
2259 1104 977
AgeI A'CCGG_T
Cuts at: 2728 2728
Size: 4340
AlwNI CAG_nnn'CTG
Cuts at: 1038 1092 1161 1645 3212 1038
Size: 54 69 484 1567 2166
Fragments arranged by size:
2166 1567 484 69 54
ApaLI G'TGCA_C
Cuts at: 3110 3110
Size: 4340
ApoI r'AATT_y
Cuts at: 196 1344 3693 3949 196
Size: 1148 2349 256 587
Fragments arranged by size:
2349 1148 587 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4340
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
AvaI C'yCGr_G
Cuts at: 172 503 569 1011 1360 172
Size: 331 66 442 349 3152
Fragments arranged by size:
3152 442 349 331 66
BamHI G'GATC_C
Cuts at: 1028 1365 1028
Size: 337 4003
BanI G'GyrC_C
Cuts at: 426 876 1356 1770 2018 2053 426
Size: 450 480 414 248 35 2713
Fragments arranged by size:
2713 480 450 414 248 35
BanII G_rGCy'C
Cuts at: 350 1354 2384 350
Size: 1004 1030 2306
Fragments arranged by size:
2306 1030 1004
BbeI G_GCGC'C
Cuts at: 1774 2022 1774
Size: 248 4092
BbsI GAAGACnn'nnnn_
Cuts at: 692 692
Size: 4340
BbvCI CC'TCA_GC
Cuts at: 980 980
Size: 4340
BciVI GTATCCnnnnn_n'
Cuts at: 2238 2999 4340 2238
Size: 761 1341 2238
Fragments arranged by size:
2238 1341 761
BclI T'GATC_A
Cuts at: 1860 1860
Size: 4340
BglI GCCn_nnn'nGGC
Cuts at: 760 760
Size: 4340
BglII A'GATC_T
Cuts at: 1855 3892 1855
Size: 2037 2303
BlpI GC'TnA_GC
Cuts at: 1452 1452
Size: 4340
Bme1580I G_kGCm'C
Cuts at: 1965 2058 3114 1965
Size: 93 1056 3191
Fragments arranged by size:
3191 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1102 1552 1633 1968 4112 1102
Size: 450 81 335 2144 1330
Fragments arranged by size:
2144 1330 450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 952 1075 1186 943
Size: 9 123 111 4097
Fragments arranged by size:
4097 123 111 9
Bpu10I CC'TnA_GC
Cuts at: 980 980
Size: 4340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1516 2395 2887 3185 1516
Size: 879 492 298 2671
Fragments arranged by size:
2671 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1136 1241 4044 1136
Size: 105 2803 1432
Fragments arranged by size:
2803 1432 105
BsaAI yAC'GTr
Cuts at: 1693 1693
Size: 4340
BsaBI GATnn'nnATC
Cuts at: 6 1854 3891 6
Size: 1848 2037 455
Fragments arranged by size:
2037 1848 455
BsaHI Gr'CG_yC
Cuts at: 892 1771 2019 2721 4217 892
Size: 879 248 702 1496 1015
Fragments arranged by size:
1496 1015 879 702 248
BsaWI w'CCGG_w
Cuts at: 191 350 1319 1529 2050 2728 3002 3149 3560
Size: 159 969 210 521 678 274 147 411
Cuts at: 3560 191
Size: 971
Fragments arranged by size:
971 969 678 521 411 274 210 159
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 556 940 1072 556
Size: 384 132 3824
Fragments arranged by size:
3824 384 132
BseYI C'CCAG_C
Cuts at: 185 755 1182 1765 2523 3100 3596 185
Size: 570 427 583 758 577 496 929
Fragments arranged by size:
929 758 583 577 570 496 427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 149
Size: 4340
BsiEI CG_ry'CG
Cuts at: 104 1882 1928 3136 104
Size: 1778 46 1208 1308
Fragments arranged by size:
1778 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 1354 2132 2322 3114 350
Size: 1004 778 190 792 1576
Fragments arranged by size:
1576 1004 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1162 1162
Size: 4340
Bsp1286I G_dGCh'C
Cuts at: 350 1354 1965 2058 2132 2322 2384 3114 350
Size: 1004 611 93 74 190 62 730 1576
Fragments arranged by size:
1576 1004 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 191 350 3560 191
Size: 159 3210 971
Fragments arranged by size:
3210 971 159
BspHI T'CATG_A
Cuts at: 3516 3516
Size: 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1277 1390 1604 1906 2287 1277
Size: 113 214 302 381 3330
Fragments arranged by size:
3330 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 781 2632 2686 4170 4335 50
Size: 731 1851 54 1484 165 55
Fragments arranged by size:
1851 1484 731 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2252 2252
Size: 4340
BsrFI r'CCGG_y
Cuts at: 770 2338 2519 2728 770
Size: 1568 181 209 2382
Fragments arranged by size:
2382 1568 209 181
BsrGI T'GTAC_A
Cuts at: 1253 1253
Size: 4340
BssSI C'ACGA_G
Cuts at: 2611 2969 2611
Size: 358 3982
BstAPI GCAn_nnn'nTGC
Cuts at: 1161 1717 1161
Size: 556 3784
BstBI TT'CG_AA
Cuts at: 2701 2701
Size: 4340
BstEII G'GTnAC_C
Cuts at: 531 531
Size: 4340
BstYI r'GATC_y
Cuts at: 1 929 1028 1365 1832 1855 2190 2436 3448
Size: 928 99 337 467 23 335 246 1012
Cuts at: 3448 3534 3546 3892 1
Size: 86 12 346 449
Fragments arranged by size:
1012 928 467 449 346 337 335 246
99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 143 698 143
Size: 555 3785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2290 2471 2634 2290
Size: 181 163 3996
Fragments arranged by size:
3996 181 163
BtsI GCAGTG_nn'
Cuts at: 455 1583 455
Size: 1128 3212
DraI TTT'AAA
Cuts at: 1339 1339
Size: 4340
DrdI GACnn_nn'nnGTC
Cuts at: 2046 2904 2046
Size: 858 3482
EaeI y'GGCC_r
Cuts at: 1925 2099 2490 2517 3862 1925
Size: 174 391 27 1345 2403
Fragments arranged by size:
2403 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1925 1925
Size: 4340
EarI CTCTTCn'nnn_
Cuts at: 338 455 1318 2363 2573 338
Size: 117 863 1045 210 2105
Fragments arranged by size:
2105 1045 863 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 117 789 1124 2548 2858 3004 4150 117
Size: 672 335 1424 310 146 1146 307
Fragments arranged by size:
1424 1146 672 335 310 307 146
EcoICRI GAG'CTC
Cuts at: 348 1352 348
Size: 1004 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 952 1075 1186 2165 2597 3344 3612 943
Size: 9 123 111 979 432 747 268 1671
Fragments arranged by size:
1671 979 747 432 268 123 111 9
EcoO109I rG'GnC_Cy
Cuts at: 139 1035 1119 1479 139
Size: 896 84 360 3000
Fragments arranged by size:
3000 896 360 84
EcoRI G'AATT_C
Cuts at: 1344 1344
Size: 4340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 4340
FspI TGC'GCA
Cuts at: 2121 2121
Size: 4340
HaeII r_GCGC'y
Cuts at: 1774 2022 3044 4023 1774
Size: 248 1022 979 2091
Fragments arranged by size:
2091 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 919 921 937 951 953 969 1132
Size: 32 433 2 16 14 2 16 163
Cuts at: 1132 1164 1180 1212 2182 2212 2214 2244 454
Size: 32 16 32 970 30 2 30 2550
Fragments arranged by size:
2550 970 433 163 32 32 32 30
30 16 16 16 14 2 2 2
HincII GTy'rAC
Cuts at: 735 890 1379 735
Size: 155 489 3696
Fragments arranged by size:
3696 489 155
KasI G'GCGC_C
Cuts at: 1770 2018 1770
Size: 248 4092
KpnI G_GTAC'C
Cuts at: 1360 1360
Size: 4340
MluI A'CGCG_T
Cuts at: 3773 3773
Size: 4340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3195 3011
Size: 184 4156
MscI TGG'CCA
Cuts at: 2101 2101
Size: 4340
MslI CAynn'nnrTG
Cuts at: 2329 2456 2329
Size: 127 4213
MspA1I CmG'CkG
Cuts at: 759 911 942 972 1020 1158 1451 1765 2125
Size: 152 31 30 48 138 293 314 360
Cuts at: 2125 3138 3383 759
Size: 1013 245 1716
Fragments arranged by size:
1716 1013 360 314 293 245 152 138
48 31 30
NaeI GCC'GGC
Cuts at: 772 2521 772
Size: 1749 2591
NarI GG'CG_CC
Cuts at: 1771 2019 1771
Size: 248 4092
NgoMIV G'CCGG_C
Cuts at: 770 2519 770
Size: 1749 2591
NspI r_CATG'y
Cuts at: 1393 1893 2800 1393
Size: 500 907 2933
Fragments arranged by size:
2933 907 500
PciI A'CATG_T
Cuts at: 2796 2796
Size: 4340
PflMI CCAn_nnn'nTGG
Cuts at: 1038 1038
Size: 4340
PfoI T'CCnGG_A
Cuts at: 1024 1031 1024
Size: 7 4333
PmeI GTTT'AAAC
Cuts at: 1339 1339
Size: 4340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 4308
PpuMI rG'GwC_Cy
Cuts at: 139 1035 1119 139
Size: 896 84 3360
Fragments arranged by size:
3360 896 84
PstI C_TGCA'G
Cuts at: 1229 1274 1387 2072 1229
Size: 45 113 685 3497
Fragments arranged by size:
3497 685 113 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4340
PvuII CAG'CTG
Cuts at: 759 972 1020 1158 1765 2125 759
Size: 213 48 138 607 360 2974
Fragments arranged by size:
2974 607 360 213 138 48
RsrII CG'GwC_CG
Cuts at: 2535 2535
Size: 4340
SacI G_AGCT'C
Cuts at: 350 1354 350
Size: 1004 3336
SalI G'TCGA_C
Cuts at: 1377 1377
Size: 4340
SapI GCTCTTCn'nnn_
Cuts at: 338 1318 2363 2573 338
Size: 980 1045 210 2105
Fragments arranged by size:
2105 1045 980 210
SbfI CC_TGCA'GG
Cuts at: 1387 1387
Size: 4340
ScaI AGT'ACT
Cuts at: 3857 3857
Size: 4340
SexAI A'CCwGG_T
Cuts at: 1037 1037
Size: 4340
SfcI C'TryA_G
Cuts at: 33 1225 1270 1383 2068 3061 3252 33
Size: 1192 45 113 685 993 191 1121
Fragments arranged by size:
1192 1121 993 685 191 113 45
SfoI GGC'GCC
Cuts at: 1772 2020 1772
Size: 248 4092
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
SmaI CCC'GGG
Cuts at: 1013 1362 1013
Size: 349 3991
SmlI C'TyrA_G
Cuts at: 569 1495 2410 2902 3164 569
Size: 926 915 492 262 1745
Fragments arranged by size:
1745 926 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4340
SphI G_CATG'C
Cuts at: 1393 1893 1393
Size: 500 3840
SspI AAT'ATT
Cuts at: 303 303
Size: 4340
StyI C'CwwG_G
Cuts at: 665 835 1115 1474 1828 665
Size: 170 280 359 354 3177
Fragments arranged by size:
3177 359 354 280 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 894 1118 1923 2056 2515 2724 444
Size: 450 224 805 133 459 209 2060
Fragments arranged by size:
2060 805 459 450 224 209 133
TatI w'GTAC_w
Cuts at: 1253 3855 1253
Size: 2602 1738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 4308
Tth111I GACn'n_nGTC
Cuts at: 2137 2137
Size: 4340
XbaI T'CTAG_A
Cuts at: 1371 1371
Size: 4340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1835 1835
Size: 4340
XhoI C'TCGA_G
Cuts at: 569 569
Size: 4340
XmaI C'CCGG_G
Cuts at: 1011 1360 1011
Size: 349 3991
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BssHII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI
ZraI