Restriction map for
pF1KB8225
(Circular) (Six-base) MAPSORT of: pF1KB8225.seq Check: 7939 from: 1 to: 3539
pF1KB8225 3539 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 577 577
Size: 3539
Acc65I G'GTAC_C
Cuts at: 555 555
Size: 3539
AclI AA'CG_TT
Cuts at: 3379 3379
Size: 3539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1364 1796 2543 2811 227
Size: 1137 432 747 268 955
Fragments arranged by size:
1137 955 747 432 268
AflIII A'CryG_T
Cuts at: 236 891 1995 2972 236
Size: 655 1104 977 803
Fragments arranged by size:
1104 977 803 655
AgeI A'CCGG_T
Cuts at: 1927 1927
Size: 3539
AlwNI CAG_nnn'CTG
Cuts at: 844 2411 844
Size: 1567 1972
ApaLI G'TGCA_C
Cuts at: 2309 2309
Size: 3539
ApoI r'AATT_y
Cuts at: 543 2892 3148 543
Size: 2349 256 934
Fragments arranged by size:
2349 934 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
AvaI C'yCGr_G
Cuts at: 158 559 158
Size: 401 3138
BamHI G'GATC_C
Cuts at: 564 564
Size: 3539
BanI G'GyrC_C
Cuts at: 555 969 1217 1252 555
Size: 414 248 35 2842
Fragments arranged by size:
2842 414 248 35
BanII G_rGCy'C
Cuts at: 155 227 553 1583 155
Size: 72 326 1030 2111
Fragments arranged by size:
2111 1030 326 72
BbeI G_GCGC'C
Cuts at: 973 1221 973
Size: 248 3291
BbsI GAAGACnn'nnnn_
Cuts at: 296 296
Size: 3539
BbvCI CC'TCA_GC
Cuts at: 185 185
Size: 3539
BciVI GTATCCnnnnn_n'
Cuts at: 1437 2198 3539 1437
Size: 761 1341 1437
Fragments arranged by size:
1437 1341 761
BclI T'GATC_A
Cuts at: 1059 1059
Size: 3539
BfrBI ATG'CAT
Cuts at: 487 487
Size: 3539
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3539
BglII A'GATC_T
Cuts at: 1054 3091 1054
Size: 2037 1502
BlpI GC'TnA_GC
Cuts at: 651 651
Size: 3539
Bme1580I G_kGCm'C
Cuts at: 1164 1257 2313 1164
Size: 93 1056 2390
Fragments arranged by size:
2390 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 275 751 832 1167 3311 275
Size: 476 81 335 2144 503
Fragments arranged by size:
2144 503 476 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 3539
Bpu10I CC'TnA_GC
Cuts at: 185 185
Size: 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 715 1594 2086 2384 481
Size: 234 879 492 298 1636
Fragments arranged by size:
1636 879 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 3539
BsaAI yAC'GTr
Cuts at: 892 892
Size: 3539
BsaBI GATnn'nnATC
Cuts at: 6 1053 3090 6
Size: 1047 2037 455
Fragments arranged by size:
2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 970 1218 1920 3416 970
Size: 248 702 1496 1093
Fragments arranged by size:
1496 1093 702 248
BsaWI w'CCGG_w
Cuts at: 728 1249 1927 2201 2348 2759 728
Size: 521 678 274 147 411 1508
Fragments arranged by size:
1508 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 226
Size: 30 3509
BseYI C'CCAG_C
Cuts at: 964 1722 2299 2795 964
Size: 758 577 496 1708
Fragments arranged by size:
1708 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1081 1127 2335 104
Size: 977 46 1208 1308
Fragments arranged by size:
1308 1208 977 46
BsiHKAI G_wGCw'C
Cuts at: 227 553 1331 1521 2313 227
Size: 326 778 190 792 1453
Fragments arranged by size:
1453 792 778 326 190
BsmBI CGTCTCn'nnnn_
Cuts at: 155 155
Size: 3539
Bsp1286I G_dGCh'C
Cuts at: 155 227 553 1164 1257 1331 1521 1583 2313
Size: 72 326 611 93 74 190 62 730
Cuts at: 2313 155
Size: 1381
Fragments arranged by size:
1381 730 611 326 190 93 74 72
62
BspEI T'CCGG_A
Cuts at: 2759 2759
Size: 3539
BspHI T'CATG_A
Cuts at: 2715 2715
Size: 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 438 589 803 1105 1486 438
Size: 151 214 302 381 2491
Fragments arranged by size:
2491 381 302 214 151
BsrBI CCG'CTC
Cuts at: 50 1831 1885 3369 3534 50
Size: 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1451 1451
Size: 3539
BsrFI r'CCGG_y
Cuts at: 1537 1718 1927 1537
Size: 181 209 3149
Fragments arranged by size:
3149 209 181
BssSI C'ACGA_G
Cuts at: 1810 2168 1810
Size: 358 3181
BstAPI GCAn_nnn'nTGC
Cuts at: 916 916
Size: 3539
BstBI TT'CG_AA
Cuts at: 1900 1900
Size: 3539
BstYI r'GATC_y
Cuts at: 1 564 1031 1054 1389 1635 2647 2733 2745
Size: 563 467 23 335 246 1012 86 12
Cuts at: 2745 3091 1
Size: 346 449
Fragments arranged by size:
1012 563 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 173 107
Size: 66 3473
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1489 1670 1833 1489
Size: 181 163 3195
Fragments arranged by size:
3195 181 163
BtsI GCAGTG_nn'
Cuts at: 782 782
Size: 3539
DraI TTT'AAA
Cuts at: 538 538
Size: 3539
DrdI GACnn_nn'nnGTC
Cuts at: 1245 2103 1245
Size: 858 2681
EaeI y'GGCC_r
Cuts at: 251 278 1124 1298 1689 1716 3061 251
Size: 27 846 174 391 27 1345 729
Fragments arranged by size:
1345 846 729 391 174 27 27
EagI C'GGCC_G
Cuts at: 1124 1124
Size: 3539
EarI CTCTTCn'nnn_
Cuts at: 437 1562 1772 437
Size: 1125 210 2204
Fragments arranged by size:
2204 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1747 2057 2203 3349 1747
Size: 310 146 1146 1937
Fragments arranged by size:
1937 1146 310 146
EcoICRI GAG'CTC
Cuts at: 225 551 225
Size: 326 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 544 1364 1796 2543 2811 227
Size: 317 820 432 747 268 955
Fragments arranged by size:
955 820 747 432 317 268
EcoO109I rG'GnC_Cy
Cuts at: 678 678
Size: 3539
EcoRI G'AATT_C
Cuts at: 543 543
Size: 3539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1558 1558
Size: 3539
FspI TGC'GCA
Cuts at: 1320 1320
Size: 3539
HaeII r_GCGC'y
Cuts at: 973 1221 2243 3222 973
Size: 248 1022 979 1290
Fragments arranged by size:
1290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 1381 1411 1413 1443 226
Size: 32 1123 30 2 30 2322
Fragments arranged by size:
2322 1123 32 30 30 2
HincII GTy'rAC
Cuts at: 578 578
Size: 3539
KasI G'GCGC_C
Cuts at: 969 1217 969
Size: 248 3291
KpnI G_GTAC'C
Cuts at: 559 559
Size: 3539
MluI A'CGCG_T
Cuts at: 2972 2972
Size: 3539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 2210 2394 461
Size: 1749 184 1606
Fragments arranged by size:
1749 1606 184
MscI TGG'CCA
Cuts at: 253 1300 253
Size: 1047 2492
MslI CAynn'nnrTG
Cuts at: 1528 1655 1528
Size: 127 3412
MspA1I CmG'CkG
Cuts at: 184 650 964 1324 2337 2582 184
Size: 466 314 360 1013 245 1141
Fragments arranged by size:
1141 1013 466 360 314 245
NaeI GCC'GGC
Cuts at: 1720 1720
Size: 3539
NarI GG'CG_CC
Cuts at: 970 1218 970
Size: 248 3291
NcoI C'CATG_G
Cuts at: 107 107
Size: 3539
NgoMIV G'CCGG_C
Cuts at: 1718 1718
Size: 3539
NsiI A_TGCA'T
Cuts at: 489 489
Size: 3539
NspI r_CATG'y
Cuts at: 240 592 1092 1999 240
Size: 352 500 907 1780
Fragments arranged by size:
1780 907 500 352
PciI A'CATG_T
Cuts at: 236 1995 236
Size: 1759 1780
PfoI T'CCnGG_A
Cuts at: 302 302
Size: 3539
PmeI GTTT'AAAC
Cuts at: 538 538
Size: 3539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 3507
PstI C_TGCA'G
Cuts at: 413 586 1271 413
Size: 173 685 2681
Fragments arranged by size:
2681 685 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3539
PvuII CAG'CTG
Cuts at: 184 964 1324 184
Size: 780 360 2399
Fragments arranged by size:
2399 780 360
RsrII CG'GwC_CG
Cuts at: 1734 1734
Size: 3539
SacI G_AGCT'C
Cuts at: 227 553 227
Size: 326 3213
SalI G'TCGA_C
Cuts at: 576 576
Size: 3539
SapI GCTCTTCn'nnn_
Cuts at: 437 1562 1772 437
Size: 1125 210 2204
Fragments arranged by size:
2204 1125 210
SbfI CC_TGCA'GG
Cuts at: 586 586
Size: 3539
ScaI AGT'ACT
Cuts at: 3056 3056
Size: 3539
SfcI C'TryA_G
Cuts at: 33 409 582 1267 2260 2451 33
Size: 376 173 685 993 191 1121
Fragments arranged by size:
1121 993 685 376 191 173
SfoI GGC'GCC
Cuts at: 971 1219 971
Size: 248 3291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
SmaI CCC'GGG
Cuts at: 561 561
Size: 3539
SmlI C'TyrA_G
Cuts at: 460 694 1609 2101 2363 460
Size: 234 915 492 262 1636
Fragments arranged by size:
1636 915 492 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3539
SphI G_CATG'C
Cuts at: 592 1092 592
Size: 500 3039
StyI C'CwwG_G
Cuts at: 107 673 1027 107
Size: 566 354 2619
Fragments arranged by size:
2619 566 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1255 1714 1923 1122
Size: 133 459 209 2738
Fragments arranged by size:
2738 459 209 133
TatI w'GTAC_w
Cuts at: 512 3054 512
Size: 2542 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3507
Tth111I GACn'n_nGTC
Cuts at: 1336 1336
Size: 3539
XbaI T'CTAG_A
Cuts at: 570 570
Size: 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 400 1034 400
Size: 634 2905
XmaI C'CCGG_G
Cuts at: 559 559
Size: 3539
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI