Restriction map for
pF1KB8218
(Circular) (Six-base) MAPSORT of: pF1KB8218.seq Check: 4563 from: 1 to: 3668
pF1KB8218 3668 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 400 400
Size: 3668
AccI GT'mk_AC
Cuts at: 413 706 413
Size: 293 3375
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1493 1925 2672 2940 662
Size: 831 432 747 268 1390
Fragments arranged by size:
1390 831 747 432 268
AflIII A'CryG_T
Cuts at: 1020 2124 3101 1020
Size: 1104 977 1587
Fragments arranged by size:
1587 1104 977
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 156 973 2540 156
Size: 817 1567 1284
Fragments arranged by size:
1567 1284 817
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 532 672 3021 3277 532
Size: 140 2349 256 923
Fragments arranged by size:
2349 923 256 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 524 688 524
Size: 164 3504
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 142 684 1098 1346 1381 142
Size: 542 414 248 35 2429
Fragments arranged by size:
2429 542 414 248 35
BanII G_rGCy'C
Cuts at: 682 1712 682
Size: 1030 2638
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BbsI GAAGACnn'nnnn_
Cuts at: 393 393
Size: 3668
BciVI GTATCCnnnnn_n'
Cuts at: 1566 2327 3668 1566
Size: 761 1341 1566
Fragments arranged by size:
1566 1341 761
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 780 780
Size: 3668
Bme1580I G_kGCm'C
Cuts at: 145 525 1293 1386 2442 145
Size: 380 768 93 1056 1371
Fragments arranged by size:
1371 1056 768 380 93
BmgBI CAC'GTC
Cuts at: 292 292
Size: 3668
BmrI ACTGGGnnnn_n'
Cuts at: 465 880 961 1296 3440 465
Size: 415 81 335 2144 693
Fragments arranged by size:
2144 693 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 531 2035 531
Size: 1504 2164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 501 181
Size: 320 3348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 844 1723 2215 2513 445
Size: 399 879 492 298 1600
Fragments arranged by size:
1600 879 492 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 360 3372 360
Size: 3012 656
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 206 1182 3219 6
Size: 200 976 2037 455
Fragments arranged by size:
2037 976 455 200
BsaHI Gr'CG_yC
Cuts at: 193 1099 1347 2049 3545 193
Size: 906 248 702 1496 316
Fragments arranged by size:
1496 906 702 316 248
BsaWI w'CCGG_w
Cuts at: 857 1378 2056 2330 2477 2888 857
Size: 521 678 274 147 411 1637
Fragments arranged by size:
1637 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1093 1851 2428 2924 1093
Size: 758 577 496 1837
Fragments arranged by size:
1837 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 682 1460 1650 2442 682
Size: 778 190 792 1908
Fragments arranged by size:
1908 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 145 525 682 1293 1386 1460 1650 1712 2442
Size: 380 157 611 93 74 190 62 730
Cuts at: 2442 145
Size: 1371
Fragments arranged by size:
1371 730 611 380 190 157 93 74
62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 400 718 932 1234 1615 400
Size: 318 214 302 381 2453
Fragments arranged by size:
2453 381 318 302 214
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BsrGI T'GTAC_A
Cuts at: 422 422
Size: 3668
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 156 1045 156
Size: 889 2779
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstYI r'GATC_y
Cuts at: 1 693 1160 1183 1518 1764 2776 2862 2874
Size: 692 467 23 335 246 1012 86 12
Cuts at: 2874 3220 1
Size: 346 449
Fragments arranged by size:
1012 692 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 911 911
Size: 3668
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DraIII CAC_nnn'GTG
Cuts at: 473 473
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 563 1253 1427 1818 1845 3190 563
Size: 690 174 391 27 1345 1041
Fragments arranged by size:
1345 1041 690 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 376 611 1691 1901 376
Size: 235 1080 210 2143
Fragments arranged by size:
2143 1080 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 501 662 1493 1925 2672 2940 181
Size: 320 161 831 432 747 268 909
Fragments arranged by size:
909 831 747 432 320 268 161
EcoO109I rG'GnC_Cy
Cuts at: 807 807
Size: 3668
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1687 1687
Size: 3668
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 1102 1350 2372 3351 1102
Size: 248 1022 979 1419
Fragments arranged by size:
1419 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 1510 1540 1542 1572 610
Size: 32 868 30 2 30 2706
Fragments arranged by size:
2706 868 32 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2523 2339
Size: 184 3484
MscI TGG'CCA
Cuts at: 565 1429 565
Size: 864 2804
MslI CAynn'nnrTG
Cuts at: 1657 1784 1657
Size: 127 3541
MspA1I CmG'CkG
Cuts at: 156 213 661 779 1093 1453 2466 2711 156
Size: 57 448 118 314 360 1013 245 1113
Fragments arranged by size:
1113 1013 448 360 314 245 118 57
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NcoI C'CATG_G
Cuts at: 107 107
Size: 3668
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NspI r_CATG'y
Cuts at: 721 1221 2128 721
Size: 500 907 2261
Fragments arranged by size:
2261 907 500
PciI A'CATG_T
Cuts at: 2124 2124
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PstI C_TGCA'G
Cuts at: 218 715 1400 218
Size: 497 685 2486
Fragments arranged by size:
2486 685 497
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 213 661 1093 1453 213
Size: 448 432 360 2428
Fragments arranged by size:
2428 448 432 360
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 214 711 1396 2389 2580 33
Size: 181 497 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 191 181
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 526 690 526
Size: 164 3504
SmlI C'TyrA_G
Cuts at: 460 823 1738 2230 2492 460
Size: 363 915 492 262 1636
Fragments arranged by size:
1636 915 492 363 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StyI C'CwwG_G
Cuts at: 107 802 1156 107
Size: 695 354 2619
Fragments arranged by size:
2619 695 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 484 1251 1384 1843 2052 271
Size: 213 767 133 459 209 1887
Fragments arranged by size:
1887 767 459 213 209 133
TatI w'GTAC_w
Cuts at: 422 3183 422
Size: 2761 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 3636
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 699 699
Size: 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1163 114
Size: 1049 2619
XmaI C'CCGG_G
Cuts at: 524 688 524
Size: 164 3504
XmnI GAAnn'nnTTC
Cuts at: 127 127
Size: 3668
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI